FastQCFastQC Report
Wed 25 May 2016
SRR1780608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7857
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3975.052819142166221No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT3955.027364133893344RNA PCR Primer, Index 15 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC540.6872852233676976No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC370.47091765304823724No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT310.3945526282296042No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC300.3818251240931653No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT270.3436426116838488No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG220.28000509100165455No Hit
ACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCT200.2545500827287769RNA PCR Primer, Index 15 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGTCTT140.17818505791014383No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGTCTT130.16545755377370497No Hit
ATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTGCTT130.16545755377370497Illumina PCR Primer Index 8 (96% over 26bp)
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA130.16545755377370497No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC120.15273004963726614No Hit
CTGAACCCTCGTGGAGCCATTCATACAGGTCCCTATTTAAGGAACAAGTG100.12727504136438844No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA90.11454753722794961No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAAGTTAATCTCGTATGCCGT90.11454753722794961No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT90.11454753722794961No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG80.10182003309151075No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC80.10182003309151075No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT80.10182003309151075No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCC80.10182003309151075No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTAGTATGCCGT80.10182003309151075No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG150.00214327270.0000124
CCAACGT150.00214327270.0000122
CCTACGG150.00214327270.0000137
ACGTTGT150.00214327270.0000125
CAACCCT150.00214327270.0000151
ACGCAAA150.00214327270.0000111
CAACTAC150.00214327270.000016
TACGGGC150.00214327270.0000139
AACCCTT150.00214327270.0000152
GCTACTA150.00214327270.0000144
CGGGCTA150.00214327270.0000141
CCCAACG150.00214327270.0000121
TACGCAA150.00214327270.0000110
TACAACC150.00214327270.0000149
AAAGGCC150.00214327270.0000115
TGTAGGC150.00214327270.0000129
AAGGCCC150.00214327270.0000116
CAAAGGC150.00214327270.0000114
CTACGGG150.00214327270.0000138
CTACGCA150.00214327270.000019
Produced by FastQC (version 0.11.5)