##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780608_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7857 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.470026727758686 34.0 34.0 34.0 31.0 34.0 2 32.95010818378516 34.0 34.0 34.0 31.0 34.0 3 33.340715285732465 34.0 34.0 34.0 33.0 34.0 4 33.392007127402316 34.0 34.0 34.0 33.0 34.0 5 33.35166093928981 34.0 34.0 34.0 34.0 34.0 6 36.99287259768359 38.0 37.0 38.0 35.0 38.0 7 36.62873870434008 38.0 37.0 38.0 34.0 38.0 8 36.61448389970727 38.0 37.0 38.0 34.0 38.0 9 36.42713503881889 38.0 37.0 38.0 34.0 38.0 10-11 36.02087310678376 38.0 36.5 38.0 32.5 38.0 12-13 35.92140766195749 38.0 36.5 38.0 31.5 38.0 14-15 36.12033855161003 38.0 36.5 38.0 33.0 38.0 16-17 36.31799669084893 38.0 37.0 38.0 33.5 38.0 18-19 36.22731322387679 38.0 37.0 38.0 34.0 38.0 20-21 35.9345806287387 38.0 36.5 38.0 32.0 38.0 22-23 36.012727504136436 38.0 36.5 38.0 32.5 38.0 24-25 35.06981036018837 37.5 35.0 38.0 28.5 38.0 26-27 35.39722540409825 37.5 36.0 38.0 31.0 38.0 28-29 35.20930380552374 37.5 35.5 38.0 29.0 38.0 30-31 34.76326842306224 37.0 35.0 38.0 27.0 38.0 32-33 35.41345297187222 37.5 36.0 38.0 31.0 38.0 34-35 35.10780196003564 37.0 35.5 38.0 27.0 38.0 36-37 35.394298078146875 38.0 36.0 38.0 31.0 38.0 38-39 34.67118493063511 37.5 35.0 38.0 26.0 38.0 40-41 34.44730813287514 37.0 34.0 38.0 25.5 38.0 42-43 35.0993381697849 37.0 35.0 38.0 29.0 38.0 44-45 35.36177930507827 37.5 36.0 38.0 29.0 38.0 46-47 35.65349369988545 38.0 36.0 38.0 31.0 38.0 48-49 35.21655848288151 37.5 35.5 38.0 29.0 38.0 50-51 35.43489881634211 37.5 36.0 38.0 31.0 38.0 52-53 35.34548809978364 38.0 36.0 38.0 31.0 38.0 54-55 35.616774850451826 38.0 36.0 38.0 31.0 38.0 56-57 35.68613974799542 38.0 36.0 38.0 31.0 38.0 58-59 35.63662975690467 38.0 36.0 38.0 31.0 38.0 60-61 35.243477154130076 38.0 35.5 38.0 29.0 38.0 62-63 35.664630266004835 38.0 36.0 38.0 31.5 38.0 64-65 35.71598574519537 38.0 36.5 38.0 31.5 38.0 66-67 34.58699249077256 37.0 34.5 38.0 26.0 38.0 68-69 33.13249331806033 37.0 32.5 38.0 21.5 38.0 70-71 32.501463662975695 37.0 31.5 38.0 16.0 38.0 72-73 31.475372279495993 37.0 26.5 38.0 11.0 38.0 74-75 32.07903780068729 37.0 30.5 38.0 11.0 38.0 76 31.711340206185568 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4935087290833593 1101 2 0.20797094877148936 1101 3 0.4493636399828915 1101 4 0.7464673322150688 1101 5 0.48604864719308694 1101 6 -0.6333074530876388 1101 7 0.4852339875330145 1101 8 1.1258503072970285 1101 9 1.1831532381955228 1101 10-11 0.5579203912099828 1101 12-13 0.16856285975077867 1101 14-15 0.773613471390064 1101 16-17 -0.022405517186626867 1101 18-19 -0.7479938198173386 1101 20-21 -0.48297989224423077 1101 22-23 1.1304469906612482 1101 24-25 -2.339978626056393 1101 26-27 -0.893315496275207 1101 28-29 0.887083518295718 1101 30-31 0.8950418524353054 1101 32-33 0.09660591176645994 1101 34-35 1.4927171020105519 1101 36-37 0.9381635119106804 1101 38-39 -0.011361265480339 1101 40-41 1.1765627953932167 1101 42-43 0.6252346792031602 1101 44-45 0.7527059977668173 1101 46-47 0.7660748655945184 1101 48-49 -1.5829075709741929 1101 50-51 -1.853727087116546 1101 52-53 -1.1378830150880859 1101 54-55 -1.3732806906335213 1101 56-57 -1.5024167904692618 1101 58-59 -2.979935140901027 1101 60-61 -1.3738131709710686 1101 62-63 -2.229896450645086 1101 64-65 -1.1825768569611625 1101 66-67 -1.1528160802694387 1101 68-69 -0.394364964342806 1101 70-71 1.7456835777043622 1101 72-73 -2.00686759369939 1101 74-75 0.3151399161213888 1101 76 -2.1357750696581412 1102 1 -3.6176023820277514 1102 2 -0.38462164382109876 1102 3 -0.21730302668377277 1102 4 0.6723932581409926 1102 5 0.5971597583042012 1102 6 0.8481740283938421 1102 7 -1.0703215680225426 1102 8 0.7925169739636999 1102 9 1.0720421270844156 1102 10-11 0.40977224306183047 1102 12-13 1.4278221190100453 1102 14-15 0.7365764343530259 1102 16-17 0.4220389272578231 1102 18-19 -0.3035493753728957 1102 20-21 0.7022052929409597 1102 22-23 1.2230395832538363 1102 24-25 -3.9325712186489845 1102 26-27 -1.1525747555344665 1102 28-29 -0.2240275928153892 1102 30-31 1.672819630213091 1102 32-33 1.2262355413960861 1102 34-35 0.5853096946031471 1102 36-37 -2.802577228830067 1102 38-39 -0.2891390432581247 1102 40-41 1.4913776102080405 1102 42-43 1.014123568092046 1102 44-45 1.9378911829520007 1102 46-47 2.3031119026315494 1102 48-49 -4.286611274677895 1102 50-51 -0.13150486489432467 1102 52-53 1.176931799726738 1102 54-55 1.1452378278849977 1102 56-57 1.2198054317529667 1102 58-59 1.3719167109508206 1102 60-61 -5.577516874674771 1102 62-63 -1.9336001543487953 1102 64-65 0.8359416615573565 1102 66-67 1.4027394752861184 1102 68-69 -0.9869575569354012 1102 70-71 -1.9024645704437901 1102 72-73 -6.95131203814384 1102 74-75 -4.740415639434161 1102 76 -2.6542935881766567 1103 1 1.6590179883426188 1103 2 1.1512579858085275 1103 3 0.7514469733162272 1103 4 0.7835043692521069 1103 5 0.5971597583042012 1103 6 0.6016462506160636 1103 7 1.1727339875330145 1103 8 0.6096466035933261 1103 9 1.0454217567140418 1103 10-11 0.8050268726914638 1103 12-13 -0.32391399210106897 1103 14-15 0.5525486565752473 1103 16-17 1.1274787420726327 1103 18-19 1.3127700690715471 1103 20-21 0.32952010775576923 1103 22-23 0.9967664351056911 1103 24-25 -2.2716915890193548 1103 26-27 0.2461520963173882 1103 28-29 1.2302548145920156 1103 30-31 1.9193474079908626 1103 32-33 1.2007725784331242 1103 34-35 1.1038282131216661 1103 36-37 0.5498533267254899 1103 38-39 1.4284535493344706 1103 40-41 0.6401044620598881 1103 42-43 0.6044013458698245 1103 44-45 2.1253911829520007 1103 46-47 1.3690841248537708 1103 48-49 -2.304551089492712 1103 50-51 0.09013865362419438 1103 52-53 1.0698716145415474 1103 54-55 1.1238257908479596 1103 56-57 1.7933008021233334 1103 58-59 1.038583377617492 1103 60-61 -3.5809890968969924 1103 62-63 -0.7298964506450858 1103 64-65 0.4597842541499517 1103 66-67 0.39116540121204224 1103 68-69 1.3157044801016369 1103 70-71 1.540822466593248 1103 72-73 -2.8332564825882827 1103 74-75 -0.8306934172119398 1103 76 -1.6160991437322139 1104 1 -0.4615177259430965 1104 2 0.35215084295138155 1104 3 0.6398398304590813 1104 4 -0.32363848789074723 1104 5 0.1685883297327706 1104 6 0.4275391077589177 1104 7 0.9495197018187298 1104 8 0.6721466035933261 1104 9 1.2731003281426112 1104 10-11 -2.264169555879967 1104 12-13 0.28099672218464633 1104 14-15 -0.4340584862818915 1104 16-17 -0.13814625792736734 1104 18-19 1.3730379262144012 1104 20-21 -0.35797989224423077 1104 22-23 0.2891771493914064 1104 24-25 0.8934869824092146 1104 26-27 1.4135628106031035 1104 28-29 2.236951243163446 1104 30-31 1.4684545508480014 1104 32-33 0.28112972129026303 1104 34-35 0.4609710702645273 1104 36-37 1.8422640410112052 1104 38-39 2.0713106921916093 1104 40-41 2.236086604917027 1104 42-43 0.3633299172983939 1104 44-45 0.8128911829520007 1104 46-47 1.5967626962823402 1104 48-49 -0.708568946635566 1104 50-51 0.9584422250527638 1104 52-53 0.29531804311297805 1104 54-55 0.8582007908479596 1104 56-57 -0.30268134073380537 1104 58-59 -0.6399880509539386 1104 60-61 1.1109751888172923 1104 62-63 0.162960692212053 1104 64-65 1.5535342541499517 1104 66-67 0.5608082583549034 1104 68-69 2.532222337244498 1104 70-71 1.7952867523075398 1104 72-73 2.4256720888402867 1104 74-75 1.6603780113594908 1104 76 -0.10270628658935976 1105 1 1.3194346550092888 1105 2 1.1783413191418575 1105 3 0.4493636399828915 1105 4 0.6168377025854426 1105 5 0.0971597583042012 1105 6 0.3370629172827293 1105 7 1.3185673208663502 1105 8 0.3221466035933247 1105 9 1.2683384233807118 1105 10-11 0.8227352060247952 1105 12-13 0.9833776745656024 1105 14-15 0.8754653232419187 1105 16-17 0.6701870754059698 1105 18-19 1.09089506907155 1105 20-21 1.1336867744224364 1105 22-23 0.9082247684390268 1105 24-25 1.3470584109806438 1105 26-27 0.9992770963173854 1105 28-29 1.6167131479253527 1105 30-31 1.3505974079908611 1105 32-33 0.9632725784331271 1105 34-35 -0.3128384535450053 1105 36-37 0.5363116600588214 1105 38-39 0.05345354933447055 1105 40-41 0.9598961287265553 1105 42-43 0.9085680125364917 1105 44-45 1.5212245162853293 1105 46-47 1.325334124853768 1105 48-49 0.7985739105072938 1105 50-51 1.3036803202908658 1105 52-53 0.14174661454154602 1105 54-55 0.14153412418129108 1105 56-57 1.5901758021233334 1105 58-59 0.43858337761749056 1105 60-61 1.233594236436339 1105 62-63 0.3534368826882428 1105 64-65 1.8285342541499503 1105 66-67 1.711998734545375 1105 68-69 0.35007948010163403 1105 70-71 0.0845724665932508 1105 72-73 -1.2957564825882812 1105 74-75 -2.134860083878607 1105 76 -2.043182477065546 1106 1 -0.07331896817912309 1106 2 1.2015297249389647 1106 3 0.6522621907075319 1106 4 0.47915654316515344 1106 5 0.11889888873898258 1106 6 -0.32090809721002245 1106 7 -0.4278094907278529 1106 8 0.2917118209846308 1106 9 -0.2113717215468256 1106 10-11 1.2567931770392917 1106 12-13 1.034102312246759 1106 14-15 0.4587986565752473 1106 16-17 0.8455493942465395 1106 18-19 0.8582863734193751 1106 20-21 1.3648461947122854 1106 22-23 1.574891435105691 1106 24-25 0.6753192805458639 1106 26-27 2.2644944876217323 1106 28-29 1.4659885102441876 1106 30-31 1.972336538425644 1106 32-33 1.3719682306070382 1106 34-35 1.9516543000781894 1106 36-37 2.0138478919428806 1106 38-39 1.5425839841170799 1106 40-41 2.5388816359729347 1106 42-43 0.5745100415219966 1106 44-45 2.0465868351259147 1106 46-47 0.5470732552885522 1106 48-49 1.7246608670290229 1106 50-51 1.7348397405807177 1106 52-53 2.4265292232372033 1106 54-55 0.19515731258708513 1106 56-57 -0.1489546326592759 1106 58-59 1.4516268558783665 1106 60-61 1.0263478596247424 1106 62-63 0.8570600710940397 1106 64-65 0.2980994715412564 1106 66-67 1.0025784446903074 1106 68-69 2.332688175753809 1106 70-71 1.5041376839845526 1106 72-73 3.0607652565421546 1106 74-75 1.416589191483716 1106 76 2.3988465084417 1107 1 0.8861013216759517 1107 2 -0.7883253475248075 1107 3 0.6826969733162258 1107 4 0.6835043692521054 1107 5 -1.069506908362463 1107 6 0.20372958394939644 1107 7 0.2519006541996802 1107 8 -0.31118672974000816 1107 9 0.9016717567140446 1107 10-11 0.3727352060247924 1107 12-13 0.6667110078989325 1107 14-15 0.4421319899085816 1107 16-17 -0.029812924594033063 1107 18-19 0.52422840240488 1107 20-21 -0.13297989224423645 1107 22-23 -0.27510856489430324 1107 24-25 -0.31960825568602047 1107 26-27 -0.6007229036826089 1107 28-29 1.1667131479253499 1107 30-31 1.3339307413241954 1107 32-33 1.27993924509979 1107 34-35 -0.09617178687833672 1107 36-37 1.1529783267254885 1107 38-39 0.37012021600113343 1107 40-41 0.6765627953932167 1107 42-43 -0.1414319874635126 1107 44-45 -0.7121088170479979 1107 46-47 1.4420007915204351 1107 48-49 1.2319072438406238 1107 50-51 1.0870136536241972 1107 52-53 0.8750799478748803 1107 54-55 1.4915341241812925 1107 56-57 0.7901758021233363 1107 58-59 0.538583377617492 1107 60-61 1.9669275697696733 1107 62-63 1.470103549354917 1107 64-65 1.3285342541499574 1107 66-67 0.8119987345453765 1107 68-69 1.5334128134349712 1107 70-71 -0.9320942000734149 1107 72-73 0.4875768507450502 1107 74-75 -0.9015267505452726 1107 76 0.12348418960112184 1108 1 -0.7989561495884168 1108 2 0.6932838478774954 1108 3 -0.18282026806308238 1108 4 0.6800560933900357 1108 5 0.3212976893386852 1108 6 0.10487900923675397 1108 7 0.07144088408473692 1108 8 0.04283625876573893 1108 9 -0.9535006570790614 1108 10-11 0.21181566579490863 1108 12-13 1.316711007898931 1108 14-15 0.8553503807131762 1108 16-17 -0.2459048786170186 1108 18-19 0.22710196562326956 1108 20-21 0.5687442456868013 1108 22-23 -0.8561430476529281 1108 24-25 1.1625756523599549 1108 26-27 -0.43865393816537335 1108 28-29 -0.5235167371321197 1108 30-31 0.05404568385292663 1108 32-33 -0.9091412146703206 1108 34-35 0.22451786829407894 1108 36-37 0.9943576370703155 1108 38-39 0.4758673424379154 1108 40-41 2.188057048266785 1108 42-43 0.03328065621465015 1108 44-45 1.8344429070899295 1108 46-47 1.128782400715835 1108 48-49 0.7089187380934945 1108 50-51 0.6111515846586713 1108 52-53 0.7158845455760243 1108 54-55 0.12544217015830839 1108 56-57 0.6591413193647142 1108 58-59 0.1592730327899048 1108 60-61 1.1312953858616268 1108 62-63 -0.05748265754164095 1108 64-65 0.5147411507016812 1108 66-67 0.4665964356948038 1108 68-69 0.6362863766533593 1108 70-71 -0.4688758092688161 1108 72-73 0.4835538622393045 1108 74-75 0.3611169276156474 1108 76 -0.6581250058011783 1109 1 0.2912295268041589 1109 2 1.1680849088854472 1109 3 0.667312357931614 1109 4 0.39888898463672007 1109 5 0.5586982198426611 1109 6 -0.8911422109223963 1109 7 -0.4378429355439053 1109 8 0.4990696805164063 1109 9 1.2016717567140418 1109 10-11 0.616324949614544 1109 12-13 1.7397879309758508 1109 14-15 1.228029425806021 1109 16-17 1.217622972841859 1109 18-19 1.4101258383023207 1109 20-21 1.5939431846788494 1109 22-23 0.7671991274133845 1109 24-25 0.9278276417498716 1109 26-27 0.6415847886250816 1109 28-29 -2.489697108484904 1109 30-31 1.0082897156831692 1109 32-33 -0.10211203695148896 1109 34-35 -1.973094863801414 1109 36-37 1.50297832672549 1109 38-39 0.45729970318062385 1109 40-41 0.6701525389829683 1109 42-43 1.4265167304852113 1109 44-45 1.2263527214135337 1109 46-47 1.2368725863922378 1109 48-49 0.7639585258919013 1109 50-51 -0.016832500221958924 1109 52-53 -1.489022616227679 1109 54-55 -1.1417992091520404 1109 56-57 -0.08290112095358637 1109 58-59 0.7501218391559519 1109 60-61 1.3348762877183944 1109 62-63 0.8470266262779944 1109 64-65 1.3131496387653385 1109 66-67 0.8286654012120422 1109 68-69 -1.9499205198983631 1109 70-71 -2.4423506103298287 1109 72-73 -3.2419103287421294 1109 74-75 -1.8835780325965565 1109 76 -3.9021568360399073 1110 1 -1.5950580986139045 1110 2 -1.0158615794088632 1110 3 0.08704479940318066 1110 4 -0.43388693509572107 1110 5 0.03194236699984998 1110 6 -0.32090809721002245 1110 7 0.44175572666345175 1110 8 0.7699726905498494 1110 9 0.3538456697575185 1110 10-11 0.06114100312624515 1110 12-13 0.8384501383337124 1110 14-15 0.7631464826622008 1110 16-17 -0.08923321444910925 1110 18-19 0.6843733299411099 1110 20-21 0.8865853251470739 1110 22-23 -1.2946737822856136 1110 24-25 -1.7812024585845734 1110 26-27 1.4166684006652162 1110 28-29 -1.3166201854079844 1110 30-31 -1.2233156354873955 1110 32-33 0.7632725784331242 1110 34-35 0.6038282131216661 1110 36-37 1.2312391962907085 1110 38-39 0.9338883319431659 1110 40-41 -0.5045966248966351 1110 42-43 0.09624917195677796 1110 44-45 1.7639781394737355 1110 46-47 0.5688123857233336 1110 48-49 2.050747823550765 1110 50-51 0.25657887101549903 1110 52-53 -0.02999251589323393 1110 54-55 -0.04397312219551708 1110 56-57 0.6119149325581148 1110 58-59 -3.3092427093390313 1110 60-61 -0.1475651838535157 1110 62-63 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-0.865427533406752 2116 72-73 1.6542435174117145 2116 74-75 0.9818065827880602 2116 76 1.3901508562677876 2117 1 0.2527679883426188 2117 2 -0.004992014191472549 2117 3 0.03269697331622723 2117 4 -0.13316229741455743 2117 5 0.3471597583042012 2117 6 0.4037295839493993 2117 7 1.2352339875330145 2117 8 -1.4111867297400096 2117 9 -1.5483282432859582 2117 10-11 -0.6272647939752005 2117 12-13 -1.933288992101069 2117 14-15 1.167131989908576 2117 16-17 -1.221479591260696 2117 18-19 -4.942438264261789 2117 20-21 -2.1913132255775665 2117 22-23 -0.8417752315609732 2117 24-25 -3.3029415890193548 2117 26-27 -2.842389570349276 2117 28-29 -2.8582868520746487 2117 30-31 0.11726407465753397 2117 32-33 -1.9867274215668758 2117 34-35 -0.18783845354500528 2117 36-37 -1.0803550066078387 2117 38-39 -0.32154645066552945 2117 40-41 -1.0317705379401119 2117 42-43 -1.2080986541301755 2117 44-45 -2.0621088170479993 2117 46-47 -1.3496658751462292 2117 48-49 -3.6847594228260476 2117 50-51 -0.404653013042477 2117 52-53 -0.049920052125116854 2117 54-55 -2.100132542485376 2117 56-57 -0.07649086454333798 2117 58-59 -0.711416622382508 2117 60-61 -2.4247390968969924 2117 62-63 0.2284368826882428 2117 64-65 -0.6547990791833769 2117 66-67 -2.6713345987879578 2117 68-69 -2.533253853231699 2117 70-71 -3.03209420007342 2117 72-73 -1.6040898159216148 2117 74-75 0.8984732494547316 2117 76 2.290150856267786 2118 1 -0.4972320116573812 2118 2 -1.5049920141914725 2118 3 -0.9673030266837728 2118 4 0.28350436925210687 2118 5 0.5971597583042012 2118 6 0.07039625061606358 2118 7 1.2352339875330145 2118 8 1.1721466035933261 2118 9 -1.0483282432859582 2118 10-11 1.1019018726914638 2118 12-13 -3.058288992101069 2118 14-15 1.2087986565752473 2118 16-17 -4.138146257927367 2118 18-19 -1.3591049309284529 2118 20-21 -0.9204798922442308 2118 22-23 -0.6126085648943089 2118 24-25 -0.7404415890193548 2118 26-27 -0.8007229036826118 2118 28-29 -1.6916201854079844 2118 30-31 -1.6744025920091374 2118 32-33 0.7007725784331242 2118 34-35 -0.14617178687833388 2118 36-37 -3.62202167327451 2118 38-39 -1.8215464506655294 2118 40-41 -0.34427053794011186 2118 42-43 1.9169013458698245 2118 44-45 -2.8746088170479993 2118 46-47 -1.0371658751462292 2118 48-49 -2.538926089492712 2118 50-51 -3.6129863463758056 2118 52-53 -3.0082533854584526 2118 54-55 -4.14179920915204 2118 56-57 -1.0348241978766666 2118 58-59 -0.836416622382508 2118 60-61 1.2002609031030076 2118 62-63 0.5826035493549142 2118 64-65 0.3660342541499517 2118 66-67 0.8286654012120422 2118 68-69 0.7375794801016369 2118 70-71 1.572072466593248 2118 72-73 4.416743517411717 2118 74-75 4.35680658278806 2118 76 4.915150856267786 2119 1 -3.997232011657381 2119 2 0.37000798580852745 2119 3 0.28269697331622723 2119 4 -0.09149563074789313 2119 5 0.5971597583042012 2119 6 -1.6796037493839364 2119 7 -1.6397660124669855 2119 8 0.7971466035933261 2119 9 0.8266717567140418 2119 10-11 0.16440187269146378 2119 12-13 -1.120788992101069 2119 14-15 1.4587986565752473 2119 16-17 0.5493537420726327 2119 18-19 1.3908950690715471 2119 20-21 1.2670201077557692 2119 22-23 0.012391435105691073 2119 24-25 2.0095584109806452 2119 26-27 0.1992770963173882 2119 28-29 -0.3166201854079844 2119 30-31 2.3880974079908626 2119 32-33 -0.11172742156687576 2119 34-35 2.103828213121666 2119 36-37 -0.9345216732745101 2119 38-39 -3.5715464506655294 2119 40-41 -2.594270537940112 2119 42-43 1.4794013458698245 2119 44-45 1.2503911829520007 2119 46-47 1.9003341248537708 2119 48-49 2.086073910507288 2119 50-51 1.7620136536241944 2119 52-53 -0.6957533854584526 2119 54-55 0.23320079084795964 2119 56-57 -3.2848241978766666 2119 58-59 0.538583377617492 2119 60-61 1.7627609031030076 2119 62-63 0.6451035493549142 2119 64-65 -0.3839657458500483 2119 66-67 0.26616540121204224 2119 68-69 0.5500794801016369 2119 70-71 -0.05292753340675205 2119 72-73 1.0417435174117173 2119 74-75 0.8568065827880602 2119 76 0.16515085626778614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 2.0 24 6.0 25 16.0 26 27.0 27 52.0 28 85.0 29 149.0 30 224.0 31 356.0 32 517.0 33 746.0 34 1088.0 35 1233.0 36 1554.0 37 1800.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.31144202621866 27.033218785796105 10.067455771923125 33.587883416062105 2 14.483899707267406 16.342115311187477 47.066310296550846 22.107674684994272 3 13.0075092274405 28.611429298714526 35.16609392898053 23.21496754486445 4 11.200203640066183 24.56408298332697 34.69517627593229 29.54053710067456 5 10.551100929107802 38.32251495481736 38.18251240931653 12.943871706758307 6 20.465826651393662 42.64986636120657 16.431207840142548 20.453099147257223 7 19.994908998345423 34.51699121802215 21.038564337533412 24.44953544609902 8 18.225785923380425 39.50617283950617 23.90225276823215 18.365788468881252 9 18.51851851851852 14.0893470790378 19.791268932162403 47.60086547028128 10-11 19.670357642866236 25.671375843197147 30.74328624156803 23.914980272368588 12-13 22.629502354588265 20.026727758686523 27.23685885197913 30.106911034746087 14-15 20.866743031691485 25.245004454626446 20.6758304696449 33.21242204403717 16-17 23.482245131729666 27.86050655466463 21.534936998854526 27.12231131475118 18-19 23.164057528318697 26.746849942726232 20.91765304823724 29.17143948071783 20-21 21.445844469899452 24.716813032964236 20.58673794068983 33.25060455644648 22-23 21.827669593992617 30.437826142293495 22.57222858597429 25.162275677739594 24-25 23.80679648720886 32.4042255313733 20.879470535827924 22.90950744558992 26-27 21.38220694921726 32.550591828942345 25.53773704976454 20.529464172075855 28-29 18.664884816087564 34.55517373043146 24.175894107165586 22.604047346315387 30-31 20.465826651393662 29.400534555173728 30.546009927453227 19.587628865979383 32-33 21.8213058419244 32.03512791141657 27.275041364388443 18.868524882270588 34-35 19.072164948453608 27.459590174366806 34.98790887107038 18.4803360061092 36-37 17.691230749649993 31.87603410971109 27.96869033982436 22.464044800814563 38-39 20.4785541555301 23.93407152857325 27.77777777777778 27.809596538118875 40-41 18.63306605574647 25.22591319842179 31.15056637393407 24.990454371897673 42-43 24.66590301641848 22.871324933180603 31.939671630393278 20.523100420007637 44-45 17.913962072037673 25.066819396716305 29.419625811378385 27.599592719867633 46-47 24.77408680157821 21.433116965763013 28.197785414280258 25.595010818378515 48-49 22.005854651902762 21.725849560901107 28.50324551355479 27.76505027364134 50-51 17.786687030673285 22.044037164312076 32.21967672139493 27.949599083619702 52-53 18.747613592974417 22.158584701540025 36.80157821051292 22.292223494972635 54-55 16.825760468372152 22.57222858597429 33.428789614356624 27.173221331296933 56-57 23.444062619320352 22.947689957999238 28.573246786305205 25.035000636375205 58-59 16.902125493190788 29.29871452208222 32.25149548173603 21.547664502990962 60-61 20.014000254550083 35.535191548937256 24.271350388188875 20.179457808323786 62-63 15.241186203385515 40.441644393534425 23.189512536591575 21.127656866488483 64-65 15.387552500954563 41.52984599719995 21.88494336260659 21.197658139238897 66-67 15.08209240168003 41.93076237749777 21.80857833778796 21.178566883034236 68-69 16.221204021891307 39.52526409571083 21.172203130966018 23.081328751431844 70-71 15.292096219931272 38.86343388061601 21.178566883034236 24.66590301641848 72-73 16.361206567392134 34.44698994527173 22.890416189385263 26.301387297950875 74-75 16.18302150948199 34.78426880488736 22.184039709812904 26.848669975817742 76 16.95303550973654 35.14063892070765 22.285859742904414 25.620465826651394 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 3.5 23 8.5 24 8.5 25 7.0 26 17.5 27 30.5 28 37.5 29 42.0 30 54.5 31 66.0 32 81.0 33 97.0 34 123.0 35 183.5 36 306.0 37 394.0 38 518.5 39 659.0 40 640.5 41 592.5 42 579.0 43 560.5 44 552.0 45 552.5 46 543.0 47 507.0 48 456.5 49 394.0 50 346.0 51 352.0 52 294.0 53 200.5 54 171.0 55 160.5 56 131.0 57 108.5 58 105.0 59 86.5 60 55.5 61 46.5 62 50.0 63 35.5 64 16.0 65 8.5 66 3.0 67 0.0 68 1.5 69 2.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 7857.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.63561155657375 #Duplication Level Percentage of deduplicated Percentage of total 1 71.45647927067506 41.898943617156675 2 16.930757542869546 19.8549064528446 3 6.707184718906014 11.798396334478808 4 2.756674625569785 6.465572101310933 5 1.0635988712828304 3.118238513427517 6 0.41241588886477104 1.4509354715540281 7 0.17364879531148253 0.7127402316405753 8 0.10853049706967659 0.5091001654575538 9 0.06511829824180596 0.3436426116838488 >10 0.2604731929672238 3.0800560010182 >50 0.021706099413935316 0.6872852233676976 >100 0.04341219882787063 10.080183276059564 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 397 5.052819142166221 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 395 5.027364133893344 RNA PCR Primer, Index 15 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 54 0.6872852233676976 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 37 0.47091765304823724 No Hit TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT 31 0.3945526282296042 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 30 0.3818251240931653 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT 27 0.3436426116838488 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 22 0.28000509100165455 No Hit ACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCT 20 0.2545500827287769 RNA PCR Primer, Index 15 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGTCTT 14 0.17818505791014383 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGTCTT 13 0.16545755377370497 No Hit ATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTGCTT 13 0.16545755377370497 Illumina PCR Primer Index 8 (96% over 26bp) CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA 13 0.16545755377370497 No Hit CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 12 0.15273004963726614 No Hit CTGAACCCTCGTGGAGCCATTCATACAGGTCCCTATTTAAGGAACAAGTG 10 0.12727504136438844 No Hit ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA 9 0.11454753722794961 No Hit CTTATACACATCTCCGAGCCCACGAGACCTAAGTTAATCTCGTATGCCGT 9 0.11454753722794961 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT 9 0.11454753722794961 No Hit GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG 8 0.10182003309151075 No Hit GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC 8 0.10182003309151075 No Hit ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT 8 0.10182003309151075 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCC 8 0.10182003309151075 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTAGTATGCCGT 8 0.10182003309151075 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012727504136438845 0.0 2 0.0 0.0 0.0 0.012727504136438845 0.0 3 0.0 0.0 0.0 0.012727504136438845 0.0 4 0.0 0.0 0.0 0.038182512409316534 0.0 5 0.0 0.0 0.0 0.038182512409316534 0.0 6 0.0 0.0 0.0 0.038182512409316534 0.0 7 0.0 0.0 0.0 0.038182512409316534 0.0 8 0.0 0.0 0.0 0.038182512409316534 0.0 9 0.0 0.0 0.0 0.06363752068219422 0.0 10 0.0 0.0 0.0 0.06363752068219422 0.0 11 0.0 0.0 0.0 0.07636502481863307 0.0 12 0.0 0.0 0.0 0.07636502481863307 0.0 13 0.0 0.0 0.0 0.07636502481863307 0.0 14 0.0 0.0 0.0 0.10182003309151076 0.0 15 0.0 0.0 0.0 0.10182003309151076 0.0 16 0.0 0.0 0.0 0.1145475372279496 0.0 17 0.0 0.0 0.0 0.1145475372279496 0.0 18 0.0 0.0 0.0 0.1145475372279496 0.0 19 0.0 0.0 0.0 0.14000254550082727 0.0 20 0.0 0.0 0.0 0.14000254550082727 0.0 21 0.0 0.0 0.0 0.15273004963726614 0.0 22 0.0 0.0 0.0 0.15273004963726614 0.0 23 0.0 0.0 0.0 0.19091256204658266 0.0 24 0.0 0.0 0.0 0.19091256204658266 0.0 25 0.0 0.0 0.0 0.19091256204658266 0.0 26 0.0 0.0 0.0 0.19091256204658266 0.0 27 0.0 0.0 0.0 0.21636757031946036 0.0 28 0.0 0.0 0.0 0.2545500827287769 0.0 29 0.0 0.0 0.0 0.2545500827287769 0.0 30 0.0 0.0 0.0 0.26727758686521574 0.0 31 0.0 0.0 0.0 0.31818760341097113 0.0 32 0.0 0.0 0.0 0.31818760341097113 0.0 33 0.0 0.0 0.0 0.36909761995672646 0.0 34 0.0 0.0 0.0 0.4454626447753596 0.0 35 0.0 0.0 0.0 0.5345551737304315 0.0 36 0.0 0.0 0.0 0.6745577192312587 0.0 37 0.0 0.0 0.0 0.7509227440498918 0.0 38 0.0 0.0 0.0 1.0054728267786688 0.0 39 0.0 0.0 0.0 1.5018454880997836 0.0 40 0.0 0.0 0.0 2.0109456535573376 0.0 41 0.0 0.0 0.0 2.443680794196258 0.0 42 0.0 0.0 0.0 2.8127784141529846 0.0 43 0.0 0.0 0.0 3.3091510754740994 0.0 44 0.0 0.0 0.0 3.7927962326587754 0.0 45 0.0 0.0 0.0 4.352806414662084 0.0 46 0.0 0.0 0.0 5.052819142166221 0.0 47 0.0 0.0 0.0 5.51100929107802 0.0 48 0.0 0.0 0.0 6.096474481354206 0.0 49 0.0 0.0 0.0 6.478299605447372 0.0 50 0.0 0.0 0.0 6.974672266768486 0.0 51 0.0 0.0 0.0 7.432862415680285 0.0 52 0.0 0.0 0.0 7.9419625811378385 0.0 53 0.0 0.0 0.0 8.514700267277586 0.0 54 0.0 0.0 0.0 8.896525391370751 0.0 55 0.0 0.0 0.0 9.507445589919817 0.0 56 0.0 0.0 0.0 10.194730813287514 0.0 57 0.0 0.0 0.0 10.67837597047219 0.0 58 0.0 0.0 0.0 11.251113656611938 0.0 59 0.0 0.0 0.0 11.811123838615247 0.0 60 0.0 0.0 0.0 12.192948962708412 0.0 61 0.0 0.0 0.0 12.498409061982946 0.0 62 0.0 0.0 0.0 12.791141657121038 0.0 63 0.0 0.0 0.0 13.22387679775996 0.0 64 0.0 0.0 0.0 13.66933944253532 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTTG 15 0.002143272 70.00001 24 CCAACGT 15 0.002143272 70.00001 22 CCTACGG 15 0.002143272 70.00001 37 ACGTTGT 15 0.002143272 70.00001 25 CAACCCT 15 0.002143272 70.00001 51 ACGCAAA 15 0.002143272 70.00001 11 CAACTAC 15 0.002143272 70.00001 6 TACGGGC 15 0.002143272 70.00001 39 AACCCTT 15 0.002143272 70.00001 52 GCTACTA 15 0.002143272 70.00001 44 CGGGCTA 15 0.002143272 70.00001 41 CCCAACG 15 0.002143272 70.00001 21 TACGCAA 15 0.002143272 70.00001 10 TACAACC 15 0.002143272 70.00001 49 AAAGGCC 15 0.002143272 70.00001 15 TGTAGGC 15 0.002143272 70.00001 29 AAGGCCC 15 0.002143272 70.00001 16 CAAAGGC 15 0.002143272 70.00001 14 CTACGGG 15 0.002143272 70.00001 38 CTACGCA 15 0.002143272 70.00001 9 >>END_MODULE