FastQCFastQC Report
Wed 25 May 2016
SRR1780607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97676
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT1630.16687825054261027No Hit
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT1010.10340308775953153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGT207.737326E-444.0961420
AATTGAG207.776626E-444.05084637
ACGCGAT207.796336E-444.02823312
CTACTGG207.796336E-444.02823310
AACGCGA207.796336E-444.02823311
GATCACC207.8557024E-443.96053330
ATAAACG207.875571E-443.938018
GATGGTC250.002317257235.27691716
ATGGTCG250.002317257235.27691717
ATTTGTC250.002317257235.27691720
CTGCTGA250.00232898235.24067736
GTAATTG250.00232898235.24067735
TAATTGA250.00232898235.24067736
AAACGCG250.002334862235.22258810
TGCTATT250.002334862235.22258813
TCTCTGT250.002340754335.20451723
GTGGATC250.002352573435.16842727
CTGTGCT250.002352573435.16842726
AGTAACC250.002352573435.16842733
TGGGTAA353.2132567E-431.4003832
Produced by FastQC (version 0.11.5)