FastQCFastQC Report
Wed 25 May 2016
SRR1780607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97676
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1200912.294729513903107No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT46264.736066177976166Illumina PCR Primer Index 11 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC19181.9636348744829846No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG11671.1947663704492402No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC5310.5436340554486261No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT4130.4228264875711536Illumina PCR Primer Index 11 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC3120.3194233998116221No Hit
ATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTTCTGCTT2830.28973340431631106Illumina PCR Primer Index 11 (96% over 28bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC2620.2682337524059134No Hit
CATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTTCTGCT2180.22318686268888982Illumina PCR Primer Index 11 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTTCT1580.1617592858020394Illumina PCR Primer Index 11 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC1000.10237929481141735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCG207.8071945E-444.0158819
CGCGTTT207.8071945E-444.0158821
TCGCGTT207.8071945E-444.0158820
ATGATTC207.826931E-443.99334340
CGACCAT207.826931E-443.99334314
CACGAGG207.826931E-443.99334314
TGGTTAC207.826931E-443.9933435
CCGTACA207.826931E-443.99334316
GCCTACC207.826931E-443.99334331
CCTGGTG207.826931E-443.99334333
GCGGATG207.826931E-443.99334336
GGTTACT207.826931E-443.9933436
TTTACAC501.3224053E-939.594012
CCGTCTT4850.038.5508744
TGAAGGT301.2935445E-436.6611228
AAGGTAG301.2935445E-436.6611230
AGTCTGT301.2935445E-436.6611242
AACGTGG250.002332226435.2307551
GGTGATC250.002332226435.2307551
CGTTTAT250.002338102635.21270423
Produced by FastQC (version 0.11.5)