##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780606_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6331 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.220502290317487 23.0 12.0 32.0 12.0 34.0 2 21.137419049123363 21.0 11.0 32.0 11.0 34.0 3 20.66435002369294 21.0 11.0 32.0 11.0 34.0 4 21.55251934923393 23.0 11.0 32.0 11.0 34.0 5 19.95640499131259 21.0 11.0 31.0 11.0 34.0 6 23.06681408940136 24.0 11.0 34.0 11.0 37.0 7 23.640657084188913 26.0 11.0 34.0 11.0 37.0 8 23.73953561838572 26.0 11.0 34.0 11.0 37.0 9 23.476228084030957 24.0 12.0 34.0 12.0 37.0 10-11 22.39369767809193 22.0 11.0 34.0 11.0 37.0 12-13 22.199336597693886 22.0 11.0 34.0 11.0 37.0 14-15 22.01800663402306 21.0 11.0 34.0 11.0 37.0 16-17 21.300505449376086 21.0 11.0 32.0 11.0 37.0 18-19 21.81258884852314 21.0 11.0 33.0 11.0 37.0 20-21 21.754462170273257 22.0 11.0 32.5 11.0 37.0 22-23 21.244274206286526 21.0 11.0 32.5 11.0 37.0 24-25 21.41517927657558 21.0 11.0 32.0 11.0 37.0 26-27 21.03980413836677 21.0 11.0 31.0 10.5 37.0 28-29 20.650608118780603 21.0 11.0 31.0 11.0 37.0 30-31 20.56381298373085 21.0 11.0 31.0 11.0 37.0 32-33 20.965566261254146 21.0 11.0 31.0 11.0 37.0 34-35 21.199020691833834 21.0 11.0 31.0 11.0 37.0 36-37 21.019744116253356 21.0 11.0 31.0 11.0 37.0 38-39 20.357447480650766 21.0 11.0 29.0 11.0 37.0 40-41 20.77136313378613 21.0 11.0 31.0 11.0 37.0 42-43 21.034433738745854 21.0 11.0 31.0 11.0 37.0 44-45 20.50394882325067 21.0 11.0 29.0 11.0 36.0 46-47 20.756278628968566 21.0 11.0 29.0 11.0 37.0 48-49 20.98965408308324 21.0 11.0 31.0 11.0 37.0 50-51 21.082530405939032 21.0 11.0 31.0 11.0 37.0 52-53 21.182751540041068 21.0 11.0 31.0 11.0 36.0 54-55 20.977096825146106 21.0 11.0 31.0 11.0 36.0 56-57 20.531669562470384 20.5 11.0 29.0 11.0 36.0 58-59 20.63741904912336 20.0 11.0 29.5 11.0 36.0 60-61 20.809745695782656 21.0 11.0 31.0 11.0 36.0 62-63 20.398436265992736 20.0 11.0 29.0 11.0 36.0 64-65 20.717343231716946 20.0 11.0 29.5 11.0 36.5 66-67 20.96896224924972 21.0 11.0 31.0 11.0 36.0 68-69 20.435792133944084 19.0 11.0 29.5 11.0 36.0 70-71 20.94139946296004 20.5 11.0 31.0 11.0 36.0 72-73 20.741825935871113 19.5 11.0 30.0 11.0 36.0 74-75 20.586795135049755 19.5 11.0 30.0 11.0 36.0 76 19.464381614278945 19.0 11.0 26.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7803565594373474 1101 2 1.0909134108834735 1101 3 3.885171292687179 1101 4 -0.943185458762084 1101 5 2.032485305801867 1101 6 3.862356771851438 1101 7 5.393385712268106 1101 8 1.8964329975512157 1101 9 0.8512406167194762 1101 10-11 4.573436354949621 1101 12-13 2.11066223417739 1101 14-15 2.086086933611174 1101 16-17 1.1976692810136491 1101 18-19 3.44808155053083 1101 20-21 -0.2918823700582962 1101 22-23 2.402527821844565 1101 24-25 2.713675799468941 1101 26-27 2.2517964786128175 1101 28-29 4.034271211866642 1101 30-31 1.9242855238292762 1101 32-33 2.8590993833737635 1101 34-35 3.1481577828459457 1101 36-37 2.1692817395588513 1101 38-39 3.41505136624189 1101 40-41 4.064507098192127 1101 42-43 2.9831694983474257 1101 44-45 3.6629085528583474 1101 46-47 0.41857354908367483 1101 48-49 -2.138389763130064 1101 50-51 -1.3894801433720083 1101 52-53 0.6517650741003962 1101 54-55 3.1508476703125936 1101 56-57 1.1706724808423523 1101 58-59 0.7268267052638464 1101 60-61 1.3360887306142502 1101 62-63 0.8938626326351446 1101 64-65 -0.6733834216699428 1101 66-67 0.953104973191131 1101 68-69 4.575218870091671 1101 70-71 4.253470939512571 1101 72-73 0.7262420218557182 1101 74-75 3.4756327010848764 1101 76 2.523134720411484 1102 1 -2.5321434405626526 1102 2 1.3617467442168056 1102 3 3.2518379593538462 1102 4 -0.5640187920954176 1102 5 -0.6133480275314653 1102 6 0.141523438518103 1102 7 -1.6524476210652281 1102 8 -2.191067002448783 1102 9 -0.5029260499471917 1102 10-11 3.131769688282951 1102 12-13 -0.03725443248927718 1102 14-15 2.1506702669445055 1102 16-17 1.4476692810136491 1102 18-19 0.7668315505308314 1102 20-21 1.7997842966083724 1102 22-23 2.285861155177898 1102 24-25 2.2699257994689397 1102 26-27 -1.1752868547205146 1102 28-29 -0.7886454548000259 1102 30-31 -0.4944644761707231 1102 32-33 -1.3409006166262358 1102 34-35 0.8106577828459471 1102 36-37 1.8588650728921827 1102 38-39 1.590051366241891 1102 40-41 -2.2771595684745414 1102 42-43 -0.5230805016525757 1102 44-45 -0.7725081138083212 1102 46-47 -1.3626764509163252 1102 48-49 -1.3821397631300627 1102 50-51 -2.199896810038677 1102 52-53 0.6975984074337305 1102 54-55 -0.2033189963540707 1102 56-57 0.30192248084235374 1102 58-59 2.4684933719305135 1102 60-61 1.500672063947583 1102 62-63 -1.354054034031524 1102 64-65 -0.7067167550032778 1102 66-67 -0.7218950268088697 1102 68-69 -2.549781129908329 1102 70-71 -0.13611239382076334 1102 72-73 2.1741586885223825 1102 74-75 -2.351450632248458 1102 76 -1.1185319462551817 1103 1 -1.6933276510889677 1103 2 -0.7862795715726669 1103 3 0.38341690672226747 1103 4 -4.350203002621733 1103 5 -2.8797953959525167 1103 6 1.1184971227286304 1103 7 -0.7083686736968069 1103 8 -2.661461739290889 1103 9 -2.394373418368243 1103 10-11 -5.12480925908547 1103 12-13 -4.624425485120856 1103 14-15 1.1194202669445055 1103 16-17 -2.9865412453021385 1103 18-19 -2.0045500284165385 1103 20-21 -1.5883735981284701 1103 22-23 -2.4378230553484173 1103 24-25 -4.017903147899478 1103 26-27 -3.1078526441941996 1103 28-29 -5.395553349536868 1103 30-31 -0.9599250024865107 1103 32-33 -6.709321669257815 1103 34-35 -3.357105375048789 1103 36-37 -3.7184375586867624 1103 38-39 -0.49547494954758164 1103 40-41 -1.086370094790329 1103 42-43 -2.9326199753367845 1103 44-45 -1.9353370611767424 1103 46-47 -4.134058029863695 1103 48-49 -2.1436529210248025 1103 50-51 -4.961409967933413 1103 52-53 -1.325427908355742 1103 54-55 -1.4105558384593344 1103 56-57 -4.281959098105016 1103 58-59 -3.8275592596484316 1103 60-61 -1.556893725526102 1103 62-63 -5.072804034031524 1103 64-65 -4.127769386582223 1103 66-67 -1.5574213425983423 1103 68-69 -0.9774127088556988 1103 70-71 -5.058809762241815 1103 72-73 -4.054459732530248 1103 74-75 -2.9155953690905605 1103 76 -2.427742472570973 1104 1 3.368591853554996 1104 2 4.306599685393277 1104 3 2.4577203122950237 1104 4 3.1234812079045824 1104 5 2.64032844305677 1104 6 1.446670497341632 1104 7 3.8365229671700654 1104 8 -0.032978767154666855 1104 9 6.302221008876337 1104 10-11 1.065593217694719 1104 12-13 -0.6383573736657482 1104 14-15 1.6194202669445055 1104 16-17 2.1388457516018846 1104 18-19 2.0774933152367154 1104 20-21 -0.9722745269210407 1104 22-23 -1.3759035507044572 1104 24-25 -0.20985361229576682 1104 26-27 -4.552125090014631 1104 28-29 -0.4853366312706129 1104 30-31 0.9007561120645704 1104 32-33 2.541452324550235 1104 34-35 -0.07537162891875937 1104 36-37 -1.8856202212254658 1104 38-39 -1.16730157493458 1104 40-41 -0.6374536861215994 1104 42-43 -1.7050657957702207 1104 44-45 -3.303758113808321 1104 46-47 -0.6696617450339701 1104 48-49 0.3145514133405243 1104 50-51 -1.00320563356809 1104 52-53 -1.0266662984486246 1104 54-55 -1.3099366434128967 1104 56-57 -3.8175628132752912 1104 58-59 -3.0241536868930154 1104 60-61 -0.23646028899359095 1104 62-63 -1.86692168109035 1104 64-65 -1.4714226373562198 1104 66-67 -1.6410126738676922 1104 68-69 -1.0424281887318578 1104 70-71 -2.421038864408999 1104 72-73 0.6576145708753245 1104 74-75 -0.40475945577787087 1104 76 -2.567061358019888 1105 1 -0.019643440562653325 1105 2 2.874246744216805 1105 3 2.2018379593538455 1105 4 2.3234812079045817 1105 5 -1.950848027531464 1105 6 1.5290234385181023 1105 7 0.6600523789347719 1105 8 1.1964329975512165 1105 9 3.984573950052809 1105 10-11 2.58176968828295 1105 12-13 2.4689955675107242 1105 14-15 1.5694202669445048 1105 16-17 2.6226692810136463 1105 18-19 2.9230815505308314 1105 20-21 1.449784296608371 1105 22-23 4.660861155177898 1105 24-25 3.2386757994689397 1105 26-27 1.0184631452794868 1105 28-29 2.867604545199974 1105 30-31 -0.2507144761707245 1105 32-33 3.609099383373767 1105 34-35 2.8981577828459457 1105 36-37 2.7026150728921827 1105 38-39 1.6150513662418895 1105 40-41 2.3478404315254586 1105 42-43 4.458169498347424 1105 44-45 3.321241886191679 1105 46-47 4.2185735490836755 1105 48-49 2.886610236869938 1105 50-51 1.9438531899613203 1105 52-53 2.235098407433732 1105 54-55 2.759181003645928 1105 56-57 1.845672480842353 1105 58-59 1.9184933719305164 1105 60-61 1.1194220639475816 1105 62-63 0.9521959659684747 1105 64-65 1.1432832449967236 1105 66-67 1.3031049731911288 1105 68-69 1.525218870091674 1105 70-71 -0.22986239382076334 1105 72-73 2.542908688522381 1105 74-75 2.3922993677515407 1105 76 3.7564680537448183 1106 1 1.4232137022944897 1106 2 1.567103887073948 1106 3 1.8232665307824192 1106 4 -0.23366164923827526 1106 5 -0.5151337418171806 1106 6 2.25759486708953 1106 7 -1.3399476210652281 1106 8 -1.5035670024487828 1106 9 0.7560025214813813 1106 10-11 1.0424839739972356 1106 12-13 2.565424138939292 1106 14-15 0.5837059812302208 1106 16-17 -0.12375929041492384 1106 18-19 1.7266529791022585 1106 20-21 2.1390700108940877 1106 22-23 5.0715754408921825 1106 24-25 0.8993900851832244 1106 26-27 1.6684631452794854 1106 28-29 4.831890259485689 1106 30-31 0.6528569524007075 1106 32-33 2.766242240516622 1106 34-35 -1.6804136457254835 1106 36-37 -2.404527784250675 1106 38-39 1.1079085090990368 1106 40-41 3.062126145811174 1106 42-43 -1.2239733587954333 1106 44-45 4.196241886191679 1106 46-47 5.061430691940821 1106 48-49 3.9616102368699373 1106 50-51 2.0009960471041772 1106 52-53 4.2065269788623 1106 54-55 2.4484667179316446 1106 56-57 3.6992439094137843 1106 58-59 0.8077790862162288 1106 60-61 1.4337077782332983 1106 62-63 4.105767394539907 1106 64-65 0.4004261021395763 1106 66-67 0.40310497319113026 1106 68-69 1.718076012948817 1106 70-71 2.0558518918935214 1106 72-73 -0.10709131147761752 1106 74-75 0.6708707963229728 1106 76 3.113610910887676 1107 1 -1.083279804199016 1107 2 -0.8484805285104677 1107 3 1.9336561411720297 1107 4 1.259844844268219 1107 5 -1.528120754804192 1107 6 0.329023438518103 1107 7 -0.7490385301561382 1107 8 -0.5944760933578728 1107 9 -4.042698777219918 1107 10-11 -3.425048493535229 1107 12-13 -3.1650953415801872 1107 14-15 -1.6078524603282212 1107 16-17 -2.415967082622714 1107 18-19 -2.474645722196442 1107 20-21 -3.4161247943007176 1107 22-23 -1.3277752084584655 1107 24-25 -3.4545060187128804 1107 26-27 -0.8997186729023312 1107 28-29 -0.4051227275272993 1107 30-31 -1.5029872034434497 1107 32-33 -2.977264252989869 1107 34-35 -1.8654785807904162 1107 36-37 -1.3882940180169072 1107 38-39 -3.1940395428490156 1107 40-41 -3.1067050230199946 1107 42-43 -0.09410322892530232 1107 44-45 -4.031030841081048 1107 46-47 -3.081426450916325 1107 48-49 -0.9247533994936958 1107 50-51 -1.174328628220497 1107 52-53 -2.978537956202633 1107 54-55 -2.334000814535891 1107 56-57 -2.956600246430373 1107 58-59 -2.383779355342213 1107 60-61 -3.7351233905978702 1107 62-63 -2.1864403976678872 1107 64-65 -1.1839894822760044 1107 66-67 -2.91507684499069 1107 68-69 -4.0611447662719655 1107 70-71 -1.0707714847298533 1107 72-73 -1.0843640387503442 1107 74-75 -0.5986097231575513 1107 76 0.1201044173811816 1108 1 2.0303565594373474 1108 2 -4.875753255783195 1108 3 -2.4981620406461538 1108 4 -1.9265187920954183 1108 5 -0.5508480275314653 1108 6 -1.5209765614818984 1108 7 -0.08994762106522813 1108 8 -2.8535670024487843 1108 9 -1.515426049947191 1108 10-11 -2.118230311717049 1108 12-13 -1.4060044324892758 1108 14-15 -2.905579733055493 1108 16-17 0.12266928101364982 1108 18-19 -1.8269184494691686 1108 20-21 -1.7752157033916305 1108 22-23 -2.0391388448221015 1108 24-25 0.4886757994689397 1108 26-27 0.16846314527948536 1108 28-29 -3.0573954548000266 1108 30-31 -1.400714476170723 1108 32-33 -1.1409006166262365 1108 34-35 -0.27684221715405144 1108 36-37 -1.4223849271078173 1108 38-39 -5.034948633758109 1108 40-41 0.07284043152546005 1108 42-43 -1.2668305016525743 1108 44-45 -0.6787581138083212 1108 46-47 -0.45642645091632517 1108 48-49 -3.5383897631300627 1108 50-51 0.8438531899613224 1108 52-53 -1.2399015925662695 1108 54-55 -1.7908189963540693 1108 56-57 -1.5543275191576456 1108 58-59 -2.8565066280694857 1108 60-61 -2.98057793605242 1108 62-63 -1.4728040340315225 1108 64-65 -1.9067167550032806 1108 66-67 -2.7968950268088726 1108 68-69 -1.6747811299083288 1108 70-71 0.22013760617923595 1108 72-73 -3.507091311477616 1108 74-75 1.0422993677515429 1108 76 0.9064680537448169 1109 1 -1.4696434405626526 1109 2 -3.375753255783195 1109 3 -1.0481620406461545 1109 4 2.1234812079045824 1109 5 -1.5008480275314646 1109 6 -0.07097656148189557 1109 7 -1.0899476210652281 1109 8 -0.00356700244878283 1109 9 1.6845739500528083 1109 10-11 1.1817696882829516 1109 12-13 2.5189955675107214 1109 14-15 4.144420266944504 1109 16-17 -0.4023307189863523 1109 18-19 3.7480815505308342 1109 20-21 1.949784296608371 1109 22-23 0.31086115517789636 1109 24-25 2.463675799468941 1109 26-27 1.3934631452794868 1109 28-29 0.44260454519997694 1109 30-31 1.1992855238292783 1109 32-33 0.23409938337376346 1109 34-35 1.1481577828459457 1109 36-37 -0.1473849271078187 1109 38-39 -0.18494863375810766 1109 40-41 0.32284043152546005 1109 42-43 2.033169498347423 1109 44-45 3.321241886191679 1109 46-47 3.493573549083674 1109 48-49 -0.013389763130064125 1109 50-51 0.9438531899613238 1109 52-53 0.8350984074337333 1109 54-55 -3.0158189963540707 1109 56-57 3.5706724808423544 1109 58-59 1.6434933719305143 1109 60-61 -1.2555779360524184 1109 62-63 -0.4478040340315239 1109 64-65 -1.8317167550032778 1109 66-67 0.2031049731911274 1109 68-69 3.3002188700916726 1109 70-71 2.945137606179234 1109 72-73 3.5929086885223853 1109 74-75 2.9172993677515393 1109 76 1.8564680537448197 1110 1 -0.2696434405626533 1110 2 0.47424674421680635 1110 3 -2.2481620406461538 1110 4 0.27348120790458097 1110 5 -1.7508480275314646 1110 6 0.2790234385181023 1110 7 1.8100523789347704 1110 8 -1.3035670024487835 1110 9 -0.01542604994719099 1110 10-11 -0.46823031171705054 1110 12-13 1.5689955675107221 1110 14-15 0.14442026694450405 1110 16-17 0.07266928101364911 1110 18-19 -0.3269184494691686 1110 20-21 0.7247842966083731 1110 22-23 -2.589138844822102 1110 24-25 -0.9863242005310617 1110 26-27 1.8684631452794882 1110 28-29 -2.0323954548000245 1110 30-31 -0.5257144761707231 1110 32-33 -1.5409006166262351 1110 34-35 0.2731577828459457 1110 36-37 -0.12238492710781657 1110 38-39 -0.9349486337581077 1110 40-41 0.07284043152546005 1110 42-43 2.408169498347423 1110 44-45 0.4712418861916774 1110 46-47 -0.9064264509163245 1110 48-49 0.18661023686993872 1110 50-51 -1.0061468100386755 1110 52-53 1.985098407433732 1110 54-55 -0.21581899635406998 1110 56-57 1.4706724808423566 1110 58-59 -1.5815066280694872 1110 60-61 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-2.3577006322484593 2116 76 3.3231347204114847 2117 1 3.9946422737230627 2117 2 -0.004324684354621411 2117 3 1.8946951022109886 2117 4 2.7663383507617247 2117 5 0.7705805438971076 2117 6 -0.9566908471961817 2117 7 -1.911376192493801 2117 8 5.1392901404083595 2117 9 1.1845739500528083 2117 10-11 1.2210554025686662 2117 12-13 0.6725669960821534 2117 14-15 4.797991695515936 2117 16-17 4.161954995299364 2117 18-19 4.119510121959401 2117 20-21 -0.21807284624877 2117 22-23 4.0715754408921825 2117 24-25 3.8993900851832244 2117 26-27 4.989891716708055 2117 28-29 4.296175973771405 2117 30-31 1.3671426666864193 2117 32-33 5.694813669088049 2117 34-35 6.141014925703093 2117 36-37 2.7026150728921827 2117 38-39 2.8579085090990333 2117 40-41 3.919269002954028 2117 42-43 3.2403123554902784 2117 44-45 4.267670457620248 2117 46-47 2.8471449776551054 2117 48-49 0.033038808298506694 2117 50-51 2.893853189961323 2117 52-53 2.1350984074337305 2117 54-55 2.519895289360214 2117 56-57 2.1992439094137843 2117 58-59 5.772064800501944 2117 60-61 4.183707778233298 2117 62-63 5.105767394539907 2117 64-65 2.40042610213958 2117 66-67 1.4745335446197032 2117 68-69 3.110933155805956 2117 70-71 5.198709034750664 2117 72-73 3.285765831379525 2117 74-75 3.6351565106086845 2117 76 -0.2435319462551817 2118 1 2.4678565594373474 2118 2 -2.5132532557831944 2118 3 -1.9981620406461538 2118 4 -3.2515187920954176 2118 5 -2.4258480275314653 2118 6 2.891523438518103 2118 7 -0.6524476210652281 2118 8 1.6839329975512172 2118 9 3.1845739500528083 2118 10-11 2.819269688282951 2118 12-13 -0.03725443248927718 2118 14-15 -0.44307973305549453 2118 16-17 -1.6148307189863509 2118 18-19 -1.3894184494691686 2118 20-21 1.5810342966083724 2118 22-23 -1.0891388448221022 2118 24-25 -3.5113242005310603 2118 26-27 -1.8627868547205146 2118 28-29 -1.319895454800026 2118 30-31 -0.6507144761707231 2118 32-33 0.25284938337376417 2118 34-35 -2.564342217154053 2118 36-37 -0.3598849271078173 2118 38-39 0.4338013662418909 2118 40-41 2.0978404315254586 2118 42-43 1.7894194983474243 2118 44-45 -1.3037581138083212 2118 46-47 0.7623235490836748 2118 48-49 1.5241102368699373 2118 50-51 0.6126031899613231 2118 52-53 0.5725984074337305 2118 54-55 -0.7658189963540707 2118 56-57 -2.1980775191576463 2118 58-59 -1.9065066280694865 2118 60-61 3.031922063947583 2118 62-63 -0.3228040340315239 2118 64-65 0.6057832449967222 2118 66-67 -0.8781450268088697 2118 68-69 -1.7685311299083288 2118 70-71 0.14513760617923666 2118 72-73 2.6741586885223825 2118 74-75 0.11729936775154215 2118 76 0.8814680537448183 2119 1 -2.3029767738959848 2119 2 3.507580077550138 2119 3 0.0018379593538462302 2119 4 -1.4598521254287498 2119 5 -0.21751469419813318 2119 6 -3.504309894815229 2119 7 -1.6732809543985603 2119 8 0.7464329975512172 2119 9 -0.23209271661385955 2119 10-11 2.256769688282951 2119 12-13 1.1606622341773871 2119 14-15 3.0360869336111698 2119 16-17 1.5310026143469848 2119 18-19 4.756414883864167 2119 20-21 7.549784296608372 2119 22-23 0.36919448851122993 2119 24-25 0.3636757994689397 2119 26-27 -0.8732035213871825 2119 28-29 2.617604545199974 2119 30-31 -0.7340478095040588 2119 32-33 0.7840993833737642 2119 34-35 2.1648244495126114 2119 36-37 0.4109484062255184 2119 38-39 3.215051366241891 2119 40-41 1.4728404315254586 2119 42-43 3.96650283168076 2119 44-45 1.9462418861916788 2119 46-47 5.543573549083675 2119 48-49 1.1282769035366016 2119 50-51 1.1438531899613231 2119 52-53 1.4684317407670662 2119 54-55 0.10918100364592931 2119 56-57 -0.6459941858243141 2119 58-59 0.010160038597181398 2119 60-61 -0.4472446027190813 2119 62-63 5.510529299301808 2119 64-65 4.7099499116633865 2119 66-67 0.15310497319113026 2119 68-69 0.03355220342500331 2119 70-71 -0.43819572715409905 2119 72-73 -1.3987579781442854 2119 74-75 2.575632701084878 2119 76 1.5064680537448183 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 1.0 12 7.0 13 75.0 14 224.0 15 384.0 16 507.0 17 683.0 18 736.0 19 817.0 20 633.0 21 500.0 22 341.0 23 239.0 24 172.0 25 107.0 26 80.0 27 81.0 28 68.0 29 46.0 30 40.0 31 44.0 32 48.0 33 52.0 34 71.0 35 88.0 36 122.0 37 165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.985553262422606 28.30608033021114 8.715669153833943 53.992697253532306 2 5.244037276891486 24.135207708102985 41.71536882009161 28.905386194913916 3 5.9232348760069495 25.635760543358078 41.39946296003791 27.04154162059706 4 3.7592797346390774 42.85262991628495 21.38682672563576 32.00126362344021 5 14.626441320486494 33.454430579687255 25.46201232032854 26.45711577949771 6 7.234244195229822 31.71694834939188 12.367714421102512 48.68109303427578 7 5.749486652977413 48.80745537829726 10.930342757858158 34.512715210867164 8 5.765281945980098 50.5923234876007 12.84157321118307 30.800821355236142 9 9.271836992576214 33.75454114673827 12.288737956089085 44.684883904596425 10-11 8.126678249881536 34.53640815037119 24.15100300110567 33.185910598641605 12-13 7.7160006318117205 29.466119096509242 23.361238350971412 39.45664192070763 14-15 12.114989733059549 32.61728005054494 18.662138682672563 36.60559153372295 16-17 15.811088295687886 31.37734954983415 18.906965724214185 33.90459643026379 18-19 12.051808561048807 28.921181487916602 18.054019902069182 40.97299004896541 20-21 11.885957984520614 29.529300268519982 19.017532775232983 39.56720897172642 22-23 17.848681093034276 29.647764966040118 16.174380034749642 36.32917390617596 24-25 13.157479071236772 35.310377507502764 15.297741273100614 36.23440214815985 26-27 11.522666245458852 35.82372453009003 19.886273890380664 32.767335334070445 28-29 14.010424893381773 31.353656610330123 23.764018322539883 30.871900173748223 30-31 13.39440846627705 30.879797820249564 24.632759437687568 31.093034275785815 32-33 10.851366292844732 35.12083399147054 22.761017216869373 31.266782498815353 34-35 10.567051018796398 37.26109619333439 19.973148001895435 32.19870478597378 36-37 12.209761491075659 31.306270731322066 22.145000789764648 34.33896698783763 38-39 13.157479071236772 26.994155741589005 27.27847101563734 32.56989417153689 40-41 10.14847575422524 28.74743326488706 25.106618227768124 35.99747275311957 42-43 6.586637182119729 25.8805875848997 28.28147212130785 39.25130311167272 44-45 7.913441794345285 26.63086400252725 31.25888485231401 34.19680935081345 46-47 8.53735586795135 30.153214342126045 27.783920391723267 33.52550939819934 48-49 8.600537039962092 33.699257621228874 22.184489022271364 35.51571631653767 50-51 8.221450007897646 32.9805717896067 19.736218606855157 39.0617595956405 52-53 6.286526615068709 32.9252882640973 21.789606697204235 38.99857842362976 54-55 6.728794819143895 30.966671931764335 20.889274996051178 41.41525825304059 56-57 11.104090980887696 31.38524719633549 19.902069183383354 37.608592639393464 58-59 9.93523929868899 30.927183699257622 18.662138682672563 40.47543831938082 60-61 7.7081029853103775 28.47891328384142 20.77870794503238 43.03427578581583 62-63 9.911546359184962 29.726741431053544 18.701626915179276 41.66008529458222 64-65 10.290633391249408 30.579687253198546 15.550465961143578 43.57921339440847 66-67 8.063497077870794 37.18211972832096 13.91565313536566 40.838730058442586 68-69 9.62723108513663 37.75864792291897 13.125888485231402 39.488232506713 70-71 7.321118306744589 40.99668298846944 12.960037908703207 38.72216079608277 72-73 7.092086558205654 41.61269941557416 14.286842520928763 37.00837150529142 74-75 9.169167588058759 42.9552993208024 13.323329647764966 34.55220344337388 76 11.02511451587427 38.65108197757068 12.809982625177696 37.51382088137735 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.5 14 2.0 15 2.5 16 4.0 17 5.0 18 5.0 19 3.0 20 5.0 21 9.0 22 12.5 23 17.5 24 22.0 25 25.0 26 26.0 27 27.0 28 38.0 29 49.0 30 57.0 31 74.0 32 85.5 33 88.0 34 100.0 35 134.0 36 180.5 37 205.0 38 215.5 39 233.0 40 281.5 41 329.0 42 335.0 43 350.0 44 385.0 45 409.5 46 414.0 47 399.0 48 404.5 49 378.5 50 332.0 51 324.5 52 314.5 53 268.5 54 225.0 55 226.0 56 207.5 57 166.0 58 144.0 59 139.0 60 126.5 61 94.0 62 69.0 63 65.5 64 52.0 65 36.0 66 29.0 67 28.0 68 24.5 69 14.0 70 6.5 71 6.0 72 7.0 73 9.5 74 9.0 75 7.0 76 4.5 77 3.0 78 2.5 79 1.0 80 1.5 81 2.0 82 2.0 83 2.0 84 1.0 85 1.5 86 2.0 87 1.0 88 1.5 89 2.0 90 1.0 91 0.0 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5054493760859263 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 6331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.71457905544148 #Duplication Level Percentage of deduplicated Percentage of total 1 98.05650824759104 94.83493918812194 2 1.0452392617997712 2.0217975043437053 3 0.4736240405030214 1.3741904912336125 4 0.32663726931242854 1.263623440214816 5 0.08165931732810713 0.39488232506713006 6 0.0 0.0 7 0.016331863465621425 0.1105670510187964 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG 7 0.1105670510187964 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0473858790080556 0.0 2 0.0 0.0 0.0 0.0473858790080556 0.0 3 0.0 0.0 0.0 0.0473858790080556 0.0 4 0.0 0.0 0.0 0.0631811720107408 0.0 5 0.0 0.0 0.0 0.1105670510187964 0.0 6 0.0 0.0 0.0 0.1105670510187964 0.0 7 0.0 0.0 0.0 0.1263623440214816 0.0 8 0.0 0.0 0.0 0.1263623440214816 0.0 9 0.0 0.0 0.0 0.1263623440214816 0.0 10 0.0 0.0 0.0 0.1421576370241668 0.0 11 0.0 0.0 0.0 0.1421576370241668 0.0 12 0.0 0.0 0.0 0.1421576370241668 0.0 13 0.0 0.0 0.0 0.1421576370241668 0.0 14 0.0 0.0 0.0 0.2053388090349076 0.0 15 0.0 0.0 0.0 0.236929395040278 0.0 16 0.0 0.0 0.0 0.236929395040278 0.0 17 0.0 0.0 0.0 0.2685199810456484 0.0 18 0.0 0.0 0.0 0.39488232506713 0.0 19 0.0 0.0 0.0 0.5370399620912968 0.0 20 0.0 0.0 0.0 0.6002211341020376 0.0 21 0.0 0.0 0.0 0.6476070131100932 0.0 22 0.0 0.0 0.0 0.7265834781235192 0.0 23 0.0 0.0 0.0 0.78976465013426 0.0 24 0.0 0.0 0.0 0.8213552361396304 0.0 25 0.0 0.0 0.0 0.8213552361396304 0.0 26 0.0 0.0 0.0 0.8213552361396304 0.0 27 0.0 0.0 0.0 0.8213552361396304 0.0 28 0.0 0.0 0.0 0.8371505291423156 0.0 29 0.0 0.0 0.0 0.8529458221450008 0.0 30 0.0 0.0 0.0 0.8529458221450008 0.0 31 0.0 0.0 0.0 0.9003317011530564 0.0 32 0.0 0.0 0.0 0.9635128731637972 0.0 33 0.0 0.0 0.0 0.9951034591691675 0.0 34 0.0 0.0 0.0 1.0740799241825936 0.0 35 0.0 0.0 0.0 1.279418733217501 0.0 36 0.0 0.0 0.0 1.4847575422524089 0.0 37 0.0 0.0 0.0 1.8322539883114832 0.0 38 0.0 0.0 0.0 2.0217975043437058 0.0 39 0.0 0.0 0.0 2.306112778392039 0.0 40 0.0 0.0 0.0 2.4640657084188913 0.0 41 0.0 0.0 0.0 2.6694045174537986 0.0 42 0.0 0.0 0.0 3.174853893539725 0.0 43 0.0 0.0 0.0 3.490759753593429 0.0 44 0.0 0.0 0.0 3.7592797346390774 0.0 45 0.0 0.0 0.0 4.0435950086874115 0.0 46 0.0 0.0 0.0 4.580634970778708 0.0 47 0.0 0.0 0.0 4.880745537829727 0.0 48 0.0 0.0 0.0 4.9913125888485235 0.0 49 0.0 0.0 0.0 5.228241983888801 0.0 50 0.0 0.0 0.0 5.370399620912968 0.0 51 0.0 0.0 0.0 5.52835255093982 0.0 52 0.0 0.0 0.0 5.544147843942505 0.0 53 0.0 0.0 0.0 5.654714894961302 0.0 54 0.0 0.0 0.0 5.702100773969357 0.0 55 0.0 0.0 0.0 5.9074395830042645 0.0 56 0.0 0.0 0.0 6.096983099036487 0.0 57 0.0 0.0 0.0 6.270731322066024 0.0 58 0.0 0.0 0.0 6.412888959090191 0.0 59 0.0 0.0 0.0 6.586637182119729 0.0 60 0.0 0.0 0.0 6.697204233138525 0.0 61 0.0 0.0 0.0 6.760385405149266 0.0 62 0.0 0.0 0.0 6.902543042173432 0.0 63 0.0 0.0 0.0 7.044700679197599 0.0 64 0.0 0.0 0.0 7.044700679197599 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATAAG 20 0.0066399905 52.494045 70 >>END_MODULE