FastQCFastQC Report
Wed 25 May 2016
SRR1780606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6331
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT247339.0617595956405No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT5508.687411151476859Illumina PCR Primer Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG2634.154162059706207No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC2423.8224609066498183No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT1171.8480492813141685No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1111.7532775232980573No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT961.5163481282577793No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT791.2478281472121309No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT741.1688516821987047No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC540.8529458221450008No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGTCTT290.45806349707787075No Hit
CTTATACACATCTCCGAGCCCACGCGACTTGCTAAGATCTCGTATGCCGT280.4422682040751856No Hit
ACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCT240.37908703206444483Illumina PCR Primer Index 9 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGCCTTGCTAAGATCTCGTATGCCGT240.37908703206444483No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTCAGATCTCGTATGCCGT220.3474964460590744No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG210.3317011530563892No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCG190.3001105670510188No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGTCTT180.28431527404833357No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGTCTT170.2685199810456484No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCG170.2685199810456484No Hit
CTTATACACATCTACGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT160.25272468804296316No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC160.25272468804296316No Hit
CTTATACACATCTCCGAGCACACGAGACTTGCTAAGATCTCGTATGCCGT150.23692939504027802No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTAGTATGCCGT150.23692939504027802No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT140.2211341020375928No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC140.2211341020375928No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGT130.20533880903490762No Hit
CTTATACACATCTCCGAGCCCACGTGACTTGCTAAGATCTCGTATGCCGT130.20533880903490762No Hit
CTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTGCTTGA120.18954351603222241Illumina PCR Primer Index 9 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCC120.18954351603222241No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT120.18954351603222241No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCC120.18954351603222241No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT110.1737482230295372No Hit
ATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTGCTT110.1737482230295372RNA PCR Primer, Index 47 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACATCGTATGCCGT110.1737482230295372No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC100.157952930026852No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTACGATCTCGTATGCCGT90.14215763702416678No Hit
ATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGTCTT90.14215763702416678TruSeq Adapter, Index 9 (95% over 23bp)
TCTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT90.14215763702416678No Hit
CATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTGCT90.14215763702416678Illumina PCR Primer Index 9 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAAATCGTATGCCGT90.14215763702416678No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT90.14215763702416678No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG80.12636234402148158No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCC80.12636234402148158No Hit
CTTATACACATCTCCGAGCCAACGAGACTTGCTAAGATCTCGTATGCCGT80.12636234402148158No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG80.12636234402148158No Hit
CTTATACACATCTCCGAGCCCACGAGAATTGCTAAGATCTCGTATGCCGT80.12636234402148158No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT80.12636234402148158No Hit
CTTATACACATATCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT70.1105670510187964No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA70.1105670510187964No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCC70.1105670510187964No Hit
AGATTGTTTCTGCGGAAACAAAACTTATCCTGCCATTATCTTCTTAAACT70.1105670510187964No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTCTGCCGT70.1105670510187964No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATTTCGTATGCCGT70.1105670510187964No Hit
CTCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG70.1105670510187964No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTCAGATCTCGTATGCCGTCTT70.1105670510187964TruSeq Adapter, Index 9 (95% over 23bp)
CTTCTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT70.1105670510187964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA252.5265872E-670.01
TTACACA252.5265872E-670.03
AAAATAC150.00212332970.069
TTTACAC252.5265872E-670.02
TAAGATA252.2125969E-455.99999633
AAGATAT252.2125969E-455.99999634
AAATACA200.00663701352.570
AGATATC200.00663701352.535
TTATACA4450.051.910112
CTTATAC4450.051.910111
TATACAC4800.048.1253
CTCTTAT702.7102942E-1045.01
ATACACA5450.042.385324
CGTATGC5550.041.62162441
TCGTATG5500.041.36363640
CTAAGAT5000.041.332
TGCCGTC5550.040.99099345
ATGCCGT5550.040.99099344
GCCGTCT5550.040.99099346
GTATGCC5650.040.88495642
Produced by FastQC (version 0.11.5)