##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780606_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6331 Sequences flagged as poor quality 0 Sequence length 76 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.86210709208656 34.0 34.0 34.0 31.0 34.0 2 33.25462012320329 34.0 34.0 34.0 32.0 34.0 3 33.500710788185124 34.0 34.0 34.0 34.0 34.0 4 33.48207234244195 34.0 34.0 34.0 34.0 34.0 5 33.54272626757226 34.0 34.0 34.0 34.0 34.0 6 37.3152740483336 38.0 37.0 38.0 36.0 38.0 7 36.816458695308796 38.0 37.0 38.0 34.0 38.0 8 36.88485231401042 38.0 37.0 38.0 35.0 38.0 9 36.64302637813932 38.0 37.0 38.0 34.0 38.0 10-11 36.32648870636551 38.0 37.0 38.0 34.0 38.0 12-13 36.05101879639867 38.0 37.0 38.0 33.0 38.0 14-15 36.149423471805406 38.0 36.5 38.0 33.0 38.0 16-17 35.979387142631495 38.0 36.0 38.0 31.5 38.0 18-19 35.880745537829725 38.0 36.5 38.0 31.5 38.0 20-21 35.81669562470384 38.0 36.0 38.0 31.0 38.0 22-23 36.08482072342442 38.0 36.5 38.0 32.5 38.0 24-25 34.79102827357447 37.0 34.5 38.0 28.0 38.0 26-27 34.86953087979782 37.0 34.0 38.0 29.0 38.0 28-29 35.299241825935866 37.5 36.0 38.0 31.0 38.0 30-31 34.88848523140104 37.0 35.5 38.0 27.0 38.0 32-33 35.45300900331701 37.0 36.0 38.0 31.0 38.0 34-35 34.49818354130469 37.0 34.5 38.0 25.5 38.0 36-37 34.291028273574476 37.0 34.0 38.0 26.0 38.0 38-39 29.879323961459484 34.0 25.0 37.0 11.0 38.0 40-41 32.74901279418733 36.5 30.5 37.0 18.5 38.0 42-43 34.953403885642075 37.0 34.5 38.0 29.0 38.0 44-45 35.31598483651872 37.0 35.5 38.0 31.0 38.0 46-47 35.48144053072184 38.0 36.0 38.0 31.0 38.0 48-49 35.12162375612068 37.5 35.0 38.0 29.0 38.0 50-51 34.90933501816458 37.0 34.5 38.0 29.0 38.0 52-53 34.389669878376246 37.0 34.0 38.0 25.0 38.0 54-55 35.00702890538619 37.0 35.5 38.0 27.0 38.0 56-57 35.02637813931449 37.0 35.5 38.0 27.0 38.0 58-59 34.55267730216396 37.0 34.0 38.0 26.5 38.0 60-61 34.267809192860526 37.0 34.0 38.0 25.0 38.0 62-63 34.946611909650926 37.0 35.5 38.0 27.0 38.0 64-65 35.06041699573527 37.0 36.0 38.0 29.0 38.0 66-67 33.24214184173116 37.0 32.5 38.0 22.0 38.0 68-69 30.04217343231717 35.0 24.0 37.5 11.0 38.0 70-71 26.552203443373877 29.0 17.0 35.5 10.5 37.5 72-73 21.52598325698942 22.0 11.0 29.0 10.5 36.5 74-75 20.782735744748067 21.0 11.0 27.5 11.0 36.5 76 21.3947243721371 21.0 11.0 30.0 11.0 36.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7549807806753464 1101 2 0.8860704547430629 1101 3 0.3490439579611859 1101 4 0.42495920877051674 1101 5 0.45738533810168747 1101 6 -2.7807565397776486 1101 7 0.22197013456280956 1101 8 -0.4251975415817739 1101 9 -0.32489954315865077 1101 10-11 -0.1506919721168316 1101 12-13 0.8448973316686619 1101 14-15 0.6404958837488977 1101 16-17 -0.5012984204975481 1101 18-19 -1.8416878992587726 1101 20-21 -0.9922947742108192 1101 22-23 1.1338501263347567 1101 24-25 -1.346273530809441 1101 26-27 -0.03246455749001598 1101 28-29 1.6043892658520136 1101 30-31 1.259771307485991 1101 32-33 1.8816681841459797 1101 34-35 1.926151384011483 1101 36-37 2.3977906756284852 1101 38-39 4.245437781998302 1101 40-41 2.5484799930778195 1101 42-43 2.180510225668172 1101 44-45 -0.07024571304349791 1101 46-47 1.308020286048027 1101 48-49 -1.965963629808435 1101 50-51 -0.1588765086437931 1101 52-53 -1.2098227536160309 1101 54-55 0.9627602885454891 1101 56-57 0.11616308737204406 1101 58-59 0.7093767258984371 1101 60-61 -0.17472814118059432 1101 62-63 -1.6862992667106127 1101 64-65 -1.466533513672779 1101 66-67 -1.1532337709974385 1101 68-69 -2.871772301836362 1101 70-71 -0.6168679575769715 1101 72-73 1.5555981386446156 1101 74-75 3.6240908073287486 1101 76 -1.543744864526598 1102 1 -3.157519219324655 1102 2 -0.3639295452569371 1102 3 -0.38845604203881123 1102 4 0.4374592087705196 1102 5 -0.35511466189831253 1102 6 -1.1807565397776472 1102 7 -2.0238631987705205 1102 8 0.12480245841822324 1102 9 -0.22489954315864935 1102 10-11 1.2243080278831684 1102 12-13 1.1865639983353304 1102 14-15 -1.1532541162511052 1102 16-17 0.4487015795024547 1102 18-19 -1.3062712325921098 1102 20-21 0.0306218924558479 1102 22-23 -1.4682332069985762 1102 24-25 -6.908773530809441 1102 26-27 -1.809547890823353 1102 28-29 -2.3914440674813235 1102 30-31 1.166021307485991 1102 32-33 -1.0474984825206874 1102 34-35 0.05531805067814588 1102 36-37 -3.452209324371516 1102 38-39 -0.4212288846683627 1102 40-41 0.8838966597444866 1102 42-43 -0.7319897743318222 1102 44-45 0.3526709536231678 1102 46-47 0.5059369527146984 1102 48-49 -2.165963629808438 1102 50-51 -1.417209841977126 1102 52-53 -2.5639894202827023 1102 54-55 1.5752602885454863 1102 56-57 0.5494964207053812 1102 58-59 -0.96562327410156 1102 60-61 -7.158061474513929 1102 62-63 -3.911299266710607 1102 64-65 0.06888315299388381 1102 66-67 2.430099562335897 1102 68-69 -4.35093896850303 1102 70-71 -2.4481179575769723 1102 72-73 -0.5777351946887173 1102 74-75 -0.09882585933791788 1102 76 -1.9770781978599317 1103 1 0.7865597280437626 1103 2 0.8860704547430629 1103 3 0.49641237901381885 1103 4 0.6249592087705196 1103 5 0.45738533810168747 1103 6 0.8192434602223528 1103 7 1.130742064387377 1103 8 1.3221708794708533 1103 9 0.8014162463150356 1103 10-11 -0.8085867089589343 1103 12-13 0.3378797878090154 1103 14-15 0.30891693638047 1103 16-17 0.3302805268708795 1103 18-19 1.3039261358289451 1103 20-21 0.03226662929795765 1103 22-23 0.9742010035277389 1103 24-25 -4.267326162388386 1103 26-27 -0.6763242066128328 1103 28-29 0.06579277462393662 1103 30-31 1.6281923601175734 1103 32-33 1.6202646753740524 1103 34-35 0.8086075243623512 1103 36-37 -2.1232619559504613 1103 38-39 -1.5001762530894176 1103 40-41 0.8361992913234317 1103 42-43 0.8699839098786981 1103 44-45 2.196420953623168 1103 46-47 1.4483711632410134 1103 48-49 -1.8238583666505406 1103 50-51 0.8920006843386616 1103 52-53 1.0971947902436128 1103 54-55 0.5522339727560137 1103 56-57 1.5494964207053812 1103 58-59 0.7251661995826453 1103 60-61 -4.908061474513929 1103 62-63 -0.286299266710607 1103 64-65 2.2317121003623086 1103 66-67 -0.5304267534535754 1103 68-69 2.3299820841285452 1103 70-71 4.34628993715987 1103 72-73 -2.946156247320296 1103 74-75 -0.863628490916863 1103 76 -4.424446618912562 1104 1 1.154980780675345 1104 2 0.7095998665077659 1104 3 0.5490439579611888 1104 4 0.03672391465287461 1104 5 -0.9543793677806676 1104 6 0.5251258131635268 1104 7 0.9356956247588926 1104 8 1.1395083407711653 1104 9 1.1574533980178217 1104 10-11 -1.1212802074109476 1104 12-13 0.6645051748059245 1104 14-15 0.49931941316066286 1104 16-17 0.7281133442083387 1104 18-19 1.3132140615255423 1104 20-21 -1.5373928134265071 1104 22-23 0.5005167930014238 1104 24-25 -0.669802942574151 1104 26-27 2.034202109176647 1104 28-29 2.0534088736951475 1104 30-31 1.024477189838933 1104 32-33 1.0444132821851966 1104 34-35 -0.9189466552042092 1104 36-37 0.6213200873931939 1104 38-39 1.7258299388610503 1104 40-41 4.179852542097429 1104 42-43 -0.8018427155082932 1104 44-45 1.1081856595055157 1104 46-47 1.6805693056558724 1104 48-49 -2.724787159220206 1104 50-51 0.2666136874346421 1104 52-53 0.6529223444231818 1104 54-55 1.4392308767807833 1104 56-57 0.22596700894067112 1104 58-59 -2.620035038807444 1104 60-61 1.8860561725448974 1104 62-63 -0.31571103141649104 1104 64-65 1.0413096235821158 1104 66-67 -2.055194555311161 1104 68-69 0.28509044326167654 1104 70-71 -1.1815738399299143 1104 72-73 -2.4600881358651883 1104 74-75 -2.20728174169086 1104 76 -1.6535487860952252 1105 1 1.154980780675345 1105 2 0.5360704547430615 1105 3 0.3990439579611902 1105 4 0.3749592087705196 1105 5 -0.39261466189831395 1105 6 0.41924346022235426 1105 7 0.038636801229479545 1105 8 -1.0751975415817796 1105 9 0.02510045684135065 1105 10-11 -0.625691972116833 1105 12-13 -1.1134360016646667 1105 14-15 0.4154958837488891 1105 16-17 -1.3262984204975439 1105 18-19 -0.30002123259211544 1105 20-21 0.7243718924558493 1105 22-23 0.5755167930014267 1105 24-25 0.2537264691905534 1105 26-27 0.18420210917665258 1105 28-29 1.4710559325186736 1105 30-31 0.059771307485988245 1105 32-33 0.9650015174793154 1105 34-35 -1.7321819493218484 1105 36-37 -1.5022093243715133 1105 38-39 -1.496228884668362 1105 40-41 1.9401466597444852 1105 42-43 0.3305102256681778 1105 44-45 0.5464209536231692 1105 46-47 0.6996869527146927 1105 48-49 -0.9409636298084365 1105 50-51 1.1827901580228755 1105 52-53 0.9485105797173006 1105 54-55 1.5877602885454891 1105 56-57 1.5994964207053783 1105 58-59 1.3593767258984428 1105 60-61 -0.35806147451393144 1105 62-63 -1.5612992667106056 1105 64-65 0.900133152993881 1105 66-67 -1.2449004376641 1105 68-69 0.38656103149697074 1105 70-71 2.408132042423027 1105 72-73 2.6222648053112856 1105 74-75 2.6574241406620835 1105 76 4.672921802140067 1106 1 -0.6307335050389398 1106 2 0.3860704547430629 1106 3 0.5490439579611888 1106 4 0.12495920877051958 1106 5 0.45738533810168747 1106 6 0.46210060307949874 1106 7 -1.4970774844848052 1106 8 0.44623102984679264 1106 9 -0.5820424003015034 1106 10-11 0.8493080278831684 1106 12-13 1.2758497126210457 1106 14-15 0.7619244551774642 1106 16-17 1.3415587223596006 1106 18-19 1.117835910265029 1106 20-21 0.6958004638844173 1106 22-23 0.5005167930014238 1106 24-25 0.4751550406191285 1106 26-27 2.1056306806052163 1106 28-29 2.5281987896615377 1106 30-31 1.3311998789145605 1106 32-33 1.8007158031935973 1106 34-35 2.8856751935352847 1106 36-37 3.5835049613427685 1106 38-39 0.7216282581887796 1106 40-41 3.2008609454587713 1106 42-43 2.544795939953886 1106 44-45 2.267849525051737 1106 46-47 2.117544095571837 1106 48-49 2.2626077987629927 1106 50-51 -0.18506698483426476 1106 52-53 -1.2336322774255564 1106 54-55 0.8341888599740557 1106 56-57 2.3709249921339506 1106 58-59 1.3736624401841553 1106 60-61 3.0562242397717867 1106 62-63 2.9279864475751083 1106 64-65 0.06441886727959911 1106 66-67 1.6800995623358972 1106 68-69 5.21156103149697 1106 70-71 1.22598918528017 1106 72-73 1.3508362338827133 1106 74-75 2.307424140662082 1106 76 2.0229218021400683 1107 1 -0.11774649205192844 1107 2 -1.704838636166027 1107 3 -0.5873196784024444 1107 4 -0.8750407912294804 1107 5 0.1846580653744141 1107 6 0.31924346022235284 1107 7 0.7886368012294795 1107 8 -0.5342884506726833 1107 9 -0.452172270431376 1107 10-11 0.5538534824286216 1107 12-13 -0.43843600166466956 1107 14-15 0.3041322473852546 1107 16-17 1.1987015795024547 1107 18-19 1.7931605855897033 1107 20-21 1.3970991651831213 1107 22-23 0.5914258839105173 1107 24-25 -0.3462735308094409 1107 26-27 -0.2157978908233531 1107 28-29 0.8528741143368634 1107 30-31 1.259771307485991 1107 32-33 0.35591060838840605 1107 34-35 -0.5299092220491275 1107 36-37 1.4568815847193903 1107 38-39 0.7605892971498207 1107 40-41 0.8924193870172132 1107 42-43 1.6714193165772713 1107 44-45 0.832784589986808 1107 46-47 0.6792324072601517 1107 48-49 0.7431272792824686 1107 50-51 0.45779015802287404 1107 52-53 0.6916923978991179 1107 54-55 -0.5554215296363267 1107 56-57 0.4358600570690214 1107 58-59 1.5684676349893465 1107 60-61 2.2283021618497116 1107 62-63 0.8273370969257527 1107 64-65 1.1228604257211572 1107 66-67 -0.7062640740277359 1107 68-69 3.143379213315157 1107 70-71 -2.9395952303042456 1107 72-73 -2.5777351946887173 1107 74-75 -3.510757677519738 1107 76 -1.158896379678115 1108 1 -0.4450192193246565 1108 2 0.8360704547430657 1108 3 0.5490439579611888 1108 4 0.6249592087705196 1108 5 0.45738533810168747 1108 6 0.2692434602223557 1108 7 0.4886368012294824 1108 8 -1.0251975415817753 1108 9 -0.22489954315864935 1108 10-11 1.2993080278831712 1108 12-13 1.1615639983353248 1108 14-15 -0.6095041162511023 1108 16-17 0.14870157950245755 1108 18-19 0.9249787674078931 1108 20-21 1.174371892455845 1108 22-23 0.675516793001421 1108 24-25 2.1787264691905577 1108 26-27 -2.465797890823353 1108 28-29 -0.9539440674813235 1108 30-31 0.6347713074859911 1108 32-33 1.2900015174793111 1108 34-35 -0.8071819493218513 1108 36-37 1.4227906756284838 1108 38-39 2.478771115331636 1108 40-41 0.6151466597444895 1108 42-43 -0.5194897743318236 1108 44-45 0.8464209536231664 1108 46-47 1.6746869527147012 1108 48-49 2.084036370191562 1108 50-51 1.6327901580228712 1108 52-53 1.0735105797173006 1108 54-55 0.4877602885454806 1108 56-57 1.5494964207053812 1108 58-59 1.3093767258984457 1108 60-61 2.9169385254860742 1108 62-63 1.088700733289393 1108 64-65 2.025133152993881 1108 66-67 1.5550995623358972 1108 68-69 0.6865610314969715 1108 70-71 -5.4918679575769715 1108 72-73 -2.5277351946887165 1108 74-75 -3.442575859337918 1108 76 -2.0270781978599324 1109 1 -1.6450192193246558 1109 2 0.6360704547430629 1109 3 0.5490439579611888 1109 4 0.12495920877051958 1109 5 -0.6926146618983111 1109 6 -0.18075653977764716 1109 7 0.2386368012294824 1109 8 0.6248024584182232 1109 9 0.5751004568413478 1109 10-11 0.4493080278831698 1109 12-13 0.08656399833533612 1109 14-15 0.6654958837488962 1109 16-17 -0.7512984204975481 1109 18-19 0.34997876740789025 1109 20-21 1.0243718924558465 1109 22-23 -0.5994832069985705 1109 24-25 -3.8712735308094395 1109 26-27 -1.415797890823356 1109 28-29 -1.3289440674813235 1109 30-31 0.1347713074859911 1109 32-33 -0.45999848252068176 1109 34-35 -0.05718194932185128 1109 36-37 1.9227906756284838 1109 38-39 1.6787711153316387 1109 40-41 2.065146659744485 1109 42-43 0.7805102256681806 1109 44-45 1.1714209536231621 1109 46-47 1.5996869527146984 1109 48-49 1.084036370191562 1109 50-51 0.2577901580228712 1109 52-53 -2.1014894202827037 1109 54-55 -0.9372397114545166 1109 56-57 1.1994964207053869 1109 58-59 -1.2906232741015629 1109 60-61 1.11693852548607 1109 62-63 0.9887007332893916 1109 64-65 1.8251331529938852 1109 66-67 0.33009956233589577 1109 68-69 -1.8384389685030307 1109 70-71 1.4081320424230306 1109 72-73 -0.5277351946887165 1109 74-75 1.6324241406620814 1109 76 1.7729218021400683 1110 1 -0.14501921932465223 1110 2 0.08607045474306574 1110 3 -0.15095604203881408 1110 4 -0.17504079122947758 1110 5 0.35738533810168605 1110 6 0.6692434602223543 1110 7 1.138636801229481 1110 8 1.3748024584182232 1110 9 1.2251004568413535 1110 10-11 0.3493080278831684 1110 12-13 0.9365639983353304 1110 14-15 1.2154958837489005 1110 16-17 0.8737015795024519 1110 18-19 -0.3250212325921069 1110 20-21 1.6493718924558465 1110 22-23 -0.8994832069985748 1110 24-25 -1.5462735308094437 1110 26-27 2.384202109176641 1110 28-29 1.0960559325186736 1110 30-31 0.4847713074859925 1110 32-33 0.3400015174793154 1110 34-35 1.6178180506781459 1110 36-37 2.8727906756284867 1110 38-39 0.7287711153316359 1110 40-41 -0.009853340255510545 1110 42-43 0.5805102256681778 1110 44-45 1.871420953623165 1110 46-47 1.9746869527146984 1110 48-49 2.6340363701915592 1110 50-51 0.5327901580228769 1110 52-53 2.223510579717292 1110 54-55 -0.6122397114545137 1110 56-57 2.124496420705384 1110 58-59 -0.84062327410156 1110 60-61 -0.7330614745139314 1110 62-63 -1.411299266710607 1110 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0.6164653757563592 2116 72-73 0.08893147197794704 2116 74-75 -0.009242526004584306 2116 76 -4.477078197859932 2117 1 1.0835522092467755 2117 2 0.1717847404573476 2117 3 -0.16524175632452653 2117 4 0.4106734944848043 2117 5 0.45738533810168747 2117 6 0.17638631736520693 2117 7 0.7886368012294795 2117 8 0.8033738869896538 2117 9 -2.4391852574443647 2117 10-11 -1.9006919721168316 2117 12-13 -2.1884360016646696 2117 14-15 0.9762101694631866 2117 16-17 0.1272730080738853 2117 18-19 -2.132164089734971 2117 20-21 -0.4470566789727215 2117 22-23 0.3933739358585626 2117 24-25 -2.6677021022380103 2117 26-27 -2.6443693193947837 2117 28-29 -2.007515496052754 2117 30-31 -1.4545144067997242 2117 32-33 -0.5564270539492568 2117 34-35 0.8142466221067153 2117 36-37 -0.5950664672286621 2117 38-39 0.614485401045922 2117 40-41 2.093718088315917 2117 42-43 0.6519387970967472 2117 44-45 -1.6607219035196863 2117 46-47 -0.3110273329995863 2117 48-49 1.4768935130487009 2117 50-51 0.6006473008800128 2117 52-53 1.1592248654315895 2117 54-55 0.548474574259771 2117 56-57 0.94235356356252 2117 58-59 -1.9477661312444212 2117 60-61 0.0919385254860714 2117 62-63 -0.786299266710607 2117 64-65 -2.899866847006116 2117 66-67 -2.391329009092672 2117 68-69 0.7829896029255394 2117 70-71 -1.9168679575769723 2117 72-73 3.493693376739852 2117 74-75 3.343138426376367 2117 76 4.522921802140068 2118 1 0.09248078067534493 2118 2 0.6985704547430629 2118 3 -0.5759560420388112 2118 4 0.4999592087705196 2118 5 0.26988533810168747 2118 6 0.31924346022235284 2118 7 1.1636368012294795 2118 8 1.2498024584182232 2118 9 -3.7873995431586494 2118 10-11 0.005558027883168393 2118 12-13 -2.0634360016646696 2118 14-15 0.2842458837488948 2118 16-17 -6.020048420497545 2118 18-19 0.16247876740789025 2118 20-21 -1.031878107544152 2118 22-23 -0.24948320699857618 2118 24-25 1.497476469190559 2118 26-27 -4.934547890823353 2118 28-29 -2.5476940674813235 2118 30-31 -3.802728692514009 2118 32-33 -1.3287484825206874 2118 34-35 0.3053180506781459 2118 36-37 -4.202209324371516 2118 38-39 -3.5462288846683627 2118 40-41 -3.0223533402555134 2118 42-43 -0.9507397743318222 2118 44-45 -5.397329046376832 2118 46-47 0.4121869527146984 2118 48-49 -1.009713629808438 2118 50-51 -0.19845984197712596 2118 52-53 -3.5327394202827023 2118 54-55 -4.330989711454514 2118 56-57 -0.8880035792946188 2118 58-59 -2.09062327410156 2118 60-61 -2.3768114745139286 2118 62-63 -5.005049266710607 2118 64-65 -1.4311168470061162 2118 66-67 -3.351150437664103 2118 68-69 -1.53843896850303 2118 70-71 0.33313204242302774 2118 72-73 -0.04648519468871726 2118 74-75 0.9324241406620821 2118 76 1.9604218021400683 2119 1 0.6549807806753449 2119 2 -5.030596211923605 2119 3 -1.2009560420388112 2119 4 -1.8750407912294804 2119 5 0.29071867143502317 2119 6 -1.5140898731109829 2119 7 0.20530346789614384 2119 8 0.04146912508488754 2119 9 0.8584337901746864 2119 10-11 -0.192358638783503 2119 12-13 -1.605102668331341 2119 14-15 -1.6428374495844338 2119 16-17 -2.3429650871642167 2119 18-19 -2.1500212325921098 2119 20-21 -0.3339614408774878 2119 22-23 -0.3328165403319048 2119 24-25 1.4870598025238948 2119 26-27 -1.2991312241566817 2119 28-29 0.12938926585200505 2119 30-31 0.009771307485991088 2119 32-33 -0.19333181585401604 2119 34-35 1.4094847173448102 2119 36-37 -2.202209324371516 2119 38-39 -5.129562218001698 2119 40-41 -2.543186673588849 2119 42-43 -0.8361564409984936 2119 44-45 -1.1785790463768322 2119 46-47 -2.4003130472853016 2119 48-49 -0.24929696314176653 2119 50-51 1.1661234913562026 2119 52-53 0.29017724638396913 2119 54-55 0.2627602885454863 2119 56-57 -6.4088369126279545 2119 58-59 -3.2572899407682243 2119 60-61 -1.4913948078472643 2119 62-63 -2.7029659333772784 2119 64-65 -0.1915335136727876 2119 66-67 2.2217662290025686 2119 68-69 0.08656103149697003 2119 70-71 -1.7918679575769723 2119 72-73 -1.0777351946887173 2119 74-75 2.015757473995418 2119 76 -0.9770781978599317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 7.0 24 10.0 25 19.0 26 49.0 27 90.0 28 133.0 29 216.0 30 320.0 31 504.0 32 696.0 33 972.0 34 1303.0 35 1311.0 36 466.0 37 233.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 4.801769072816301 16.19017532775233 10.66182277681251 68.34623282261886 2 2.685199810456484 4.201547938714263 81.78802716790396 11.32522508292529 3 2.5904280524403727 16.964144684883905 70.68393618701627 9.761491075659453 4 1.9270257463275944 58.600537039962084 22.255567840783446 17.21686937292687 5 2.132364555362502 28.55788974885484 64.96604012004423 4.343705575738429 6 4.122571473700837 66.37182119728321 10.266940451745379 19.238666877270575 7 4.1067761806981515 27.452219238666874 9.098088769546674 59.34291581108829 8 3.0800821355236137 66.60875059232349 15.95324593271205 14.357921339440846 9 3.095877428526299 14.27894487442742 4.912336123835097 77.71284157321118 10-11 3.5223503395987996 37.03996209129679 39.55141367872374 19.886273890380664 12-13 9.682514610646027 6.689306586637183 39.28289369767809 44.3452851050387 14-15 12.067603854051493 10.33012162375612 10.314326330753437 67.28794819143896 16-17 36.676670352235035 33.178012952140264 3.3091138840625494 26.836202811562153 18-19 37.3005844258411 16.932554098878537 3.1985468330437525 42.568314642236615 20-21 12.588848523140102 6.736692465645238 3.617122097614911 77.05733691359976 22-23 11.775390933501818 41.83383351761175 3.482862107092086 42.90791344179434 24-25 36.34496919917864 42.426157005212445 4.1699573527088925 17.058916442900017 26-27 37.26899383983573 38.99857842362976 16.908861159374506 6.8235665771600065 28-29 12.817880271679039 11.246248617911862 36.258095087663875 39.67777602274522 30-31 31.78012952140262 10.164271047227926 48.28621070920865 9.769388722160796 32-33 15.961143579213394 13.157479071236772 36.897804454272624 33.983572895277206 34-35 6.010108987521718 69.83888801137262 16.68772705733691 7.463275943768758 36-37 33.96777760227452 42.46564523771916 12.825777918180382 10.740799241825936 38-39 12.738903806665613 13.094297899226031 34.639077554888644 39.52772073921971 40-41 5.8363607644921816 10.11688516821987 44.819143895119254 39.227610172168696 42-43 38.99857842362976 5.67840783446533 43.81614278944874 11.506870952456168 44-45 8.545253514452693 33.50181645869531 40.8782182909493 17.0747117359027 46-47 40.07265834781235 6.412888959090191 18.01453166956247 35.49992102353499 48-49 34.74174695940609 3.498657400094772 13.536566103301217 48.22302953719792 50-51 9.011214658031905 3.9330279576686147 44.93760859263939 42.11814879166009 52-53 7.92923708734797 4.304217343231717 74.15890064760701 13.6076449218133 54-55 4.904438477333755 4.217343231716948 49.86574000947718 41.01247828147212 56-57 38.44574316853578 4.95182435634181 20.478597377981362 36.12383509714105 58-59 8.253040593903018 18.02242931606381 62.69151792765756 11.033012162375613 60-61 32.84631179908387 49.22603064286842 13.260148475754225 4.667509082293477 62-63 5.054493760859264 86.14752803664508 3.617122097614911 5.180856104880745 64-65 2.1797504343705576 89.42505133470226 3.1669562470383825 5.228241983888801 66-67 2.558837466435002 87.73495498341495 3.1827515400410675 6.523456010108987 68-69 2.772073921971253 82.59358711104092 4.288422050229031 10.345916916758807 70-71 3.253830358553151 74.6564523771916 6.570841889117044 15.518875375138208 72-73 4.793871426314958 59.90364871268362 10.606539251303111 24.69594060969831 74-75 5.441478439425051 56.752487758647916 10.796082767335333 27.00995103459169 76 4.327910282735744 60.21165692623598 10.187963986731955 25.272468804296324 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 3.0 21 6.0 22 5.5 23 3.0 24 3.0 25 5.0 26 5.0 27 7.5 28 9.0 29 8.0 30 6.0 31 5.0 32 4.5 33 3.0 34 10.0 35 19.5 36 36.0 37 50.0 38 110.5 39 534.0 40 1229.0 41 1468.0 42 1375.0 43 1139.0 44 690.5 45 373.0 46 268.0 47 201.5 48 107.5 49 61.5 50 43.0 51 39.0 52 35.0 53 34.0 54 33.0 55 35.5 56 27.5 57 16.0 58 15.0 59 23.5 60 30.0 61 19.5 62 11.0 63 14.0 64 11.0 65 6.5 66 5.0 67 2.0 68 1.5 69 1.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 6331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.454904438477335 #Duplication Level Percentage of deduplicated Percentage of total 1 76.13899613899613 15.574158900647609 2 10.81081081081081 4.422682040751856 3 4.864864864864865 2.9853103775075027 4 2.084942084942085 1.7058916442900016 5 1.0810810810810811 1.105670510187964 6 0.6177606177606177 0.7581740641288897 7 0.6949806949806949 0.9951034591691675 8 0.4633204633204633 0.7581740641288897 9 0.4633204633204633 0.8529458221450008 >10 2.0077220077220077 6.728794819143895 >50 0.3088803088803089 4.785973779813616 >100 0.3088803088803089 11.577949770968251 >500 0.07722007722007722 8.687411151476859 >1k 0.07722007722007722 39.0617595956405 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 2473 39.0617595956405 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 550 8.687411151476859 Illumina PCR Primer Index 9 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 263 4.154162059706207 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 242 3.8224609066498183 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT 117 1.8480492813141685 No Hit CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC 111 1.7532775232980573 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT 96 1.5163481282577793 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT 79 1.2478281472121309 No Hit TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT 74 1.1688516821987047 No Hit CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC 54 0.8529458221450008 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGTCTT 29 0.45806349707787075 No Hit CTTATACACATCTCCGAGCCCACGCGACTTGCTAAGATCTCGTATGCCGT 28 0.4422682040751856 No Hit ACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCT 24 0.37908703206444483 Illumina PCR Primer Index 9 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGCCTTGCTAAGATCTCGTATGCCGT 24 0.37908703206444483 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTCAGATCTCGTATGCCGT 22 0.3474964460590744 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG 21 0.3317011530563892 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCG 19 0.3001105670510188 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGTCTT 18 0.28431527404833357 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGTCTT 17 0.2685199810456484 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCG 17 0.2685199810456484 No Hit CTTATACACATCTACGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 16 0.25272468804296316 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 16 0.25272468804296316 No Hit CTTATACACATCTCCGAGCACACGAGACTTGCTAAGATCTCGTATGCCGT 15 0.23692939504027802 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTAGTATGCCGT 15 0.23692939504027802 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT 14 0.2211341020375928 No Hit TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC 14 0.2211341020375928 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGT 13 0.20533880903490762 No Hit CTTATACACATCTCCGAGCCCACGTGACTTGCTAAGATCTCGTATGCCGT 13 0.20533880903490762 No Hit CTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTGCTTGA 12 0.18954351603222241 Illumina PCR Primer Index 9 (96% over 28bp) CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCC 12 0.18954351603222241 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT 12 0.18954351603222241 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCC 12 0.18954351603222241 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT 11 0.1737482230295372 No Hit ATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTGCTT 11 0.1737482230295372 RNA PCR Primer, Index 47 (96% over 28bp) CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACATCGTATGCCGT 11 0.1737482230295372 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 10 0.157952930026852 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTACGATCTCGTATGCCGT 9 0.14215763702416678 No Hit ATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGTCTT 9 0.14215763702416678 TruSeq Adapter, Index 9 (95% over 23bp) TCTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 9 0.14215763702416678 No Hit CATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTGCT 9 0.14215763702416678 Illumina PCR Primer Index 9 (96% over 26bp) CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAAATCGTATGCCGT 9 0.14215763702416678 No Hit ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 9 0.14215763702416678 No Hit CTTATTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 8 0.12636234402148158 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCC 8 0.12636234402148158 No Hit CTTATACACATCTCCGAGCCAACGAGACTTGCTAAGATCTCGTATGCCGT 8 0.12636234402148158 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 8 0.12636234402148158 No Hit CTTATACACATCTCCGAGCCCACGAGAATTGCTAAGATCTCGTATGCCGT 8 0.12636234402148158 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT 8 0.12636234402148158 No Hit CTTATACACATATCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 7 0.1105670510187964 No Hit GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA 7 0.1105670510187964 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCC 7 0.1105670510187964 No Hit AGATTGTTTCTGCGGAAACAAAACTTATCCTGCCATTATCTTCTTAAACT 7 0.1105670510187964 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTCTGCCGT 7 0.1105670510187964 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATTTCGTATGCCGT 7 0.1105670510187964 No Hit CTCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 7 0.1105670510187964 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTCAGATCTCGTATGCCGTCTT 7 0.1105670510187964 TruSeq Adapter, Index 9 (95% over 23bp) CTTCTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 7 0.1105670510187964 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0631811720107408 0.0 2 0.0 0.0 0.0 0.0631811720107408 0.0 3 0.0 0.0 0.0 0.0631811720107408 0.0 4 0.0 0.0 0.0 0.0947717580161112 0.0 5 0.0 0.0 0.0 0.1421576370241668 0.0 6 0.0 0.0 0.0 0.1421576370241668 0.0 7 0.0 0.0 0.0 0.15795293002685198 0.0 8 0.0 0.0 0.0 0.15795293002685198 0.0 9 0.0 0.0 0.0 0.15795293002685198 0.0 10 0.0 0.0 0.0 0.17374822302953719 0.0 11 0.0 0.0 0.0 0.17374822302953719 0.0 12 0.0 0.0 0.0 0.17374822302953719 0.0 13 0.0 0.0 0.0 0.17374822302953719 0.0 14 0.0 0.0 0.0 0.2527246880429632 0.0 15 0.0 0.0 0.0 0.2685199810456484 0.0 16 0.0 0.0 0.0 0.2685199810456484 0.0 17 0.0 0.0 0.0 0.2843152740483336 0.0 18 0.0 0.0 0.0 0.4264729110725004 0.0 19 0.0 0.0 0.0 0.6002211341020376 0.0 20 0.0 0.0 0.0 0.6634023061127784 0.0 21 0.0 0.0 0.0 0.7265834781235192 0.0 22 0.0 0.0 0.0 0.7423787711262044 0.0 23 0.0 0.0 0.0 0.8213552361396304 0.0 24 0.0 0.0 0.0 0.8213552361396304 0.0 25 0.0 0.0 0.0 0.8213552361396304 0.0 26 0.0 0.0 0.0 0.8213552361396304 0.0 27 0.0 0.0 0.0 0.8213552361396304 0.0 28 0.0 0.0 0.0 0.8371505291423156 0.0 29 0.0 0.0 0.0 0.8371505291423156 0.0 30 0.0 0.0 0.0 0.8529458221450008 0.0 31 0.0 0.0 0.0 0.9319222871584268 0.0 32 0.0 0.0 0.0 1.0108987521718529 0.0 33 0.0 0.0 0.0 1.0424893381772231 0.0 34 0.0 0.0 0.0 1.1214658031906493 0.0 35 0.0 0.0 0.0 1.3110093192228716 0.0 36 0.0 0.0 0.0 1.500552835255094 0.0 37 0.0 0.0 0.0 1.97441162533565 0.0 38 0.0 0.0 0.0 2.1797504343705576 0.0 39 0.0 0.0 0.0 2.432475122413521 0.0 40 0.0 0.0 0.0 2.543042173432317 0.0 41 0.0 0.0 0.0 2.7325856894645395 0.0 42 0.0 0.0 0.0 3.15905860053704 0.0 43 0.0 0.0 0.0 3.4433738745853737 0.0 44 0.0 0.0 0.0 3.7434844416363924 0.0 45 0.0 0.0 0.0 4.0751855946927815 0.0 46 0.0 0.0 0.0 4.628020849786764 0.0 47 0.0 0.0 0.0 4.864950244827042 0.0 48 0.0 0.0 0.0 4.975517295845838 0.0 49 0.0 0.0 0.0 5.212446690886116 0.0 50 0.0 0.0 0.0 5.354604327910283 0.0 51 0.0 0.0 0.0 5.512557257937135 0.0 52 0.0 0.0 0.0 5.52835255093982 0.0 53 0.0 0.0 0.0 5.623124308955931 0.0 54 0.0 0.0 0.0 5.686305480966672 0.0 55 0.0 0.0 0.0 5.8600537039962095 0.0 56 0.0 0.0 0.0 6.065392513031116 0.0 57 0.0 0.0 0.0 6.207550150055283 0.0 58 0.0 0.0 0.0 6.34970778707945 0.0 59 0.0 0.0 0.0 6.523456010108988 0.0 60 0.0 0.0 0.0 6.634023061127784 0.0 61 0.0 0.0 0.0 6.68140894013584 0.0 62 0.0 0.0 0.0 6.776180698151951 0.0 63 0.0 0.0 0.0 6.949928921181488 0.0 64 0.0 0.0 0.0 6.949928921181488 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 25 2.5265872E-6 70.0 1 TTACACA 25 2.5265872E-6 70.0 3 AAAATAC 15 0.002123329 70.0 69 TTTACAC 25 2.5265872E-6 70.0 2 TAAGATA 25 2.2125969E-4 55.999996 33 AAGATAT 25 2.2125969E-4 55.999996 34 AAATACA 20 0.006637013 52.5 70 AGATATC 20 0.006637013 52.5 35 TTATACA 445 0.0 51.91011 2 CTTATAC 445 0.0 51.91011 1 TATACAC 480 0.0 48.125 3 CTCTTAT 70 2.7102942E-10 45.0 1 ATACACA 545 0.0 42.38532 4 CGTATGC 555 0.0 41.621624 41 TCGTATG 550 0.0 41.363636 40 CTAAGAT 500 0.0 41.3 32 TGCCGTC 555 0.0 40.990993 45 ATGCCGT 555 0.0 40.990993 44 GCCGTCT 555 0.0 40.990993 46 GTATGCC 565 0.0 40.884956 42 >>END_MODULE