FastQCFastQC Report
Wed 25 May 2016
SRR1780605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47865
Sequences flagged as poor quality0
Sequence length50
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACG730.15251227410425153No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG520.10863888018385043No Hit
CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA500.10446046171524077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAC207.7553437E-444.02931610
AGTCTGT207.8358245E-443.937342
AGATAGG250.00229888635.2973822
GATAGGA250.00229888635.2973823
TATTCAC250.002346713335.1498453
CATCGGA250.002346713335.1498459
GTCCCCC250.002346713335.1498457
GAGGCCC250.002346713335.14984526
GGTGGTC353.1988966E-431.38378744
CTTATTC502.2874628E-630.8205241
CTGACCC300.00567213429.35287912
TCGGACG300.00567213429.35287911
TTATACC300.00573054429.2915362
CATGTGT300.00573054429.29153633
AGCCCGT406.991287E-427.46081431
GACCCAA406.991287E-427.46081428
AGACCCA406.991287E-427.46081427
TTTACAC756.815753E-826.3623812
TTACACA857.941708E-925.8454723
ACGAGAC609.297562E-625.73767324
Produced by FastQC (version 0.11.5)