##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780605_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47865 Sequences flagged as poor quality 0 Sequence length 50 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.50680037605766 22.0 12.0 30.0 7.0 33.0 2 20.770416797242245 23.0 10.0 30.0 7.0 33.0 3 20.92823566280163 23.0 10.0 30.0 7.0 34.0 4 22.666436853650893 25.0 10.0 35.0 7.0 37.0 5 22.471346495351508 23.0 10.0 35.0 7.0 37.0 6 22.50253838921968 24.0 10.0 35.0 8.0 37.0 7 22.64661025801734 25.0 11.0 35.0 8.0 37.0 8 22.627786482816255 25.0 11.0 33.0 8.0 37.0 9 23.449179985375537 25.0 11.0 35.0 8.0 39.0 10 23.126919460984016 25.0 10.0 35.0 8.0 39.0 11 23.115804867857516 25.0 10.0 35.0 8.0 39.0 12 23.003551655698317 25.0 10.0 35.0 8.0 39.0 13 22.976935130053274 25.0 10.0 35.0 8.0 39.0 14 23.318834221247258 24.0 10.0 35.0 8.0 40.0 15 23.03029353389742 23.0 10.0 34.0 8.0 40.0 16 22.88101953410634 23.0 10.0 34.0 8.0 40.0 17 22.981092656429542 23.0 10.0 34.0 8.0 40.0 18 22.765778752742087 23.0 10.0 34.0 7.0 40.0 19 22.74283923534942 22.0 10.0 34.0 7.0 40.0 20 22.748292071450955 23.0 10.0 34.0 7.0 40.0 21 22.601462446464012 22.0 10.0 34.0 7.0 40.0 22 22.661422751488562 22.0 10.0 34.0 7.0 40.0 23 22.48321320380236 22.0 10.0 34.0 7.0 40.0 24 21.988530241303668 20.0 8.0 34.0 7.0 40.0 25 21.949441136529824 20.0 9.0 34.0 7.0 40.0 26 21.63858769455761 19.0 8.0 33.0 7.0 40.0 27 21.38382952052648 18.0 8.0 33.0 7.0 40.0 28 21.29054632821477 18.0 8.0 32.0 7.0 40.0 29 21.115387026010655 18.0 8.0 32.0 7.0 40.0 30 20.94256763814896 18.0 8.0 32.0 7.0 40.0 31 20.929238483234094 18.0 8.0 32.0 7.0 40.0 32 20.601002820432466 17.0 8.0 32.0 7.0 40.0 33 20.582680455447612 17.0 8.0 32.0 7.0 39.0 34 20.224422855949022 16.0 8.0 31.0 7.0 39.0 35 20.070949545596992 16.0 8.0 31.0 7.0 39.0 36 19.96003342734775 16.0 8.0 31.0 7.0 39.0 37 19.765089313694766 15.0 8.0 30.0 7.0 39.0 38 19.726459834952472 15.0 8.0 30.0 7.0 39.0 39 19.44105296145409 15.0 8.0 30.0 7.0 39.0 40 19.035328528152096 15.0 8.0 30.0 7.0 39.0 41 19.083317664264076 15.0 8.0 30.0 7.0 39.0 42 19.098610675859188 15.0 8.0 30.0 7.0 39.0 43 19.137950485741147 15.0 8.0 30.0 7.0 38.0 44 19.067690379191475 15.0 8.0 30.0 7.0 38.0 45 19.165172882064137 15.0 8.0 29.0 7.0 38.0 46 18.986524600438734 14.0 8.0 29.0 7.0 38.0 47 18.892551969079705 14.0 8.0 29.0 7.0 38.0 48 18.791538702601066 14.0 8.0 29.0 7.0 38.0 49 18.576036770082524 13.0 8.0 28.0 7.0 37.0 50 18.574908597095998 13.0 8.0 28.0 7.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.474454067875584 1101 2 0.26374977322542037 1101 3 0.19175216973777154 1101 4 -0.49437478917672095 1101 5 -0.35882136914079155 1101 6 -0.3863714664145448 1101 7 -0.8307569016361036 1101 8 0.945623089540117 1101 9 -0.9672259915949937 1101 10 -0.47934143522727624 1101 11 -0.12207357921455753 1101 12 0.26939149349723124 1101 13 0.22077921549709245 1101 14 0.4622362798362616 1101 15 1.7319387029633795 1101 16 0.34848088420740453 1101 17 0.5075662017379727 1101 18 -0.4711984523789532 1101 19 -0.03092177983754496 1101 20 0.18178066776749802 1101 21 -0.6140146617023028 1101 22 -0.15741163977605765 1101 23 0.44844030340619767 1101 24 0.8949927415553951 1101 25 0.7907183955385442 1101 26 -0.21064605862869357 1101 27 0.9734273235525137 1101 28 0.073430993683953 1101 29 0.7959644474585232 1101 30 0.582274812937122 1101 31 1.561969655379773 1101 32 1.197960012729176 1101 33 -0.14839759230433103 1101 34 0.46236738835897384 1101 35 0.6544980001482408 1101 36 0.054213121414381504 1101 37 0.768519639025218 1101 38 1.308876637898301 1101 39 0.413002447956039 1101 40 0.34060606932000326 1101 41 0.2815840828223095 1101 42 1.0057230955040382 1101 43 0.9135589201842365 1101 44 -0.6936222315626424 1101 45 -0.24650287374304014 1101 46 0.7893242472011401 1101 47 0.31332433225263046 1101 48 0.5555875972854061 1101 49 1.9558037137770476 1101 50 1.8193714181835645 1102 1 1.712107493685977 1102 2 2.541902926378576 1102 3 1.4654008183864207 1102 4 1.781901487099553 1102 5 2.2410284807090584 1102 6 1.9244393443962657 1102 7 1.4556545097753073 1102 8 2.097650116567145 1102 9 2.664155389786387 1102 10 2.839727633841793 1102 11 3.481905399764422 1102 12 3.4747218238275615 1102 13 1.618677113394991 1102 14 2.6634374810374624 1102 15 3.7488305948552707 1102 16 1.8657481514746728 1102 17 3.624307943479714 1102 18 1.915813559633058 1102 19 1.7675016435858808 1102 20 3.833056944043772 1102 21 2.5451444974568567 1102 22 3.4161619337975146 1102 23 2.6864282913941864 1102 24 2.7947675163301717 1102 25 3.503556233376383 1102 26 0.35391850593587293 1102 27 1.8090129091381009 1102 28 2.041899462152422 1102 29 2.2460395225336 1102 30 2.15397150963382 1102 31 2.9711288145389325 1102 32 1.114626679395844 1102 33 1.9837345398278003 1102 34 1.395925946917533 1102 35 1.707801303451543 1102 36 2.5605945027957624 1102 37 2.0586848041903814 1102 38 2.463906667928331 1102 39 2.218558003511596 1102 40 2.2178583215722547 1102 41 2.5601126113508386 1102 42 1.8315489213298655 1102 43 2.786682043307362 1102 44 1.7065279185875077 1102 45 2.2778965256563595 1102 46 1.0348197426966337 1102 47 2.299060067988364 1102 48 2.012044053741864 1102 49 2.7602331432064773 1102 50 2.4128398866520335 1103 1 -1.8716762900978061 1103 2 -2.5160121315364847 1103 3 -1.726104973119373 1103 4 -2.9007239955259294 1103 5 -1.807234067553491 1103 6 -2.386371466414545 1103 7 -2.519248965128167 1103 8 -1.7579483390313122 1103 9 -2.03468630905531 1103 10 -2.3166430225288615 1103 11 -2.1748513569923347 1103 12 -2.1476719985662633 1103 13 -1.2998557051378299 1103 14 -2.50760499000501 1103 15 -3.0716327256080476 1103 16 -3.0983445126179916 1103 17 -2.215846496674729 1103 18 -2.3088968650773687 1103 19 -3.2606836845994494 1103 20 -2.078139967153138 1103 21 -1.4251257728134128 1103 22 -1.4780465604109807 1103 23 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1.2812933460625118 1105 33 2.2960468521401154 1105 34 0.6568118328034203 1105 35 2.2100535557037944 1105 36 1.3042131214143815 1105 37 0.14351963902521803 1105 38 0.6560988601205224 1105 39 1.413002447956039 1105 40 0.7850505137644461 1105 41 1.9899174161556452 1105 42 1.797389762170706 1105 43 1.4968922535175722 1105 44 1.320266657326247 1105 45 0.7396082373680706 1105 46 1.1643242472011401 1105 47 0.841102110030409 1105 48 2.111143152840963 1105 49 0.3585814915548262 1105 50 -0.6667396929275462 1106 1 -2.0716762900978054 1106 2 -1.6878268033511539 1106 3 -1.7778424248568214 1106 4 -1.2991795939815276 1106 5 -2.5022147625341873 1106 6 -2.399884979928057 1106 7 -2.5308319767111804 1106 8 -2.3753228564058304 1106 9 -0.633141907510911 1106 10 -2.5656777715636103 1106 11 -1.8559324380734168 1106 12 -1.849602500496765 1106 13 -2.8948364001185247 1106 14 -1.9852111676111868 1106 15 -2.2511694051447293 1106 16 -0.30992752420100445 1106 17 -1.9973136781419072 1106 18 -1.8409431971236998 1106 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2.871728478480854 2212 8 2.452202036908538 2212 9 2.1307272247792746 2212 10 2.655161488749332 2212 11 2.2383065377445064 2212 12 2.65608739992998 2212 13 2.2061593324561564 2212 14 1.4987859874385983 2212 15 1.4753597555949582 2212 16 2.237369773096294 2212 17 2.0506948567087306 2212 18 1.177193360486541 2212 19 1.5370606763028078 2212 20 1.4500555215686681 2212 21 1.3932952798181653 2212 22 1.0121790034987939 2212 23 0.9908379642249123 2212 24 0.3050804608536417 2212 25 1.4420341850122291 2212 26 2.1387691460496683 2212 27 0.7716729375876028 2212 28 2.5120274849120214 2212 29 3.4619001199731443 2212 30 2.221894695978058 2212 31 1.2666480179528712 2212 32 1.7330477320274227 2212 33 3.6747018229003494 2212 34 2.911928791867748 2212 35 1.3504044328967773 2212 36 1.9219031798939135 2212 37 2.478314960662644 2212 38 2.6634088016409905 2212 39 2.3238211614063324 2212 40 3.2331499289691266 2212 41 2.916087006798918 2212 42 3.1928575984280165 2212 43 2.8770092125819 2212 44 2.0066701660981785 2212 45 2.9983801671926322 2212 46 3.293710212113421 2212 47 2.4039676071064306 2212 48 3.7207922756479803 2212 49 3.851271550034358 2212 50 3.5540205409905816 2213 1 -2.02737249262945 2213 2 -1.3369886233990513 2213 3 -1.4490706150723547 2213 4 -2.2784816808785493 2213 5 -2.3226047727976145 2213 6 -1.4686499474272026 2213 7 -0.9487245528316031 2213 8 -2.5496300750168466 2213 9 -1.9858617159269194 2213 10 -2.88264663916539 2213 11 -2.6039652810429708 2213 12 -2.8295888159261153 2213 13 -3.5253529926604337 2213 14 -2.3274964909091693 2213 15 -1.9911625628594045 2213 16 -2.2151618724733275 2213 17 -4.122356442425179 2213 18 -2.518842615529447 2213 19 -1.50824245494303 2213 20 -2.7589717935545828 2213 21 -2.0289232411678455 2213 22 -3.023797012490544 2213 23 -0.6447383182534345 2213 24 -4.186758313296924 2213 25 -3.2076993259804425 2213 26 -1.252136916575246 2213 27 -1.6858553768694264 2213 28 -2.8084255464004357 2213 29 -3.500624863371293 2213 30 -2.3155803206775047 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1.3900855030711057 2215 4 2.627847433045499 2215 5 0.8022897419703163 2215 6 1.6869618669187894 2215 7 0.9331319872527857 2215 8 -0.5960435771265509 2215 9 1.68721845284945 2215 10 0.986214120328281 2215 11 1.431815309674331 2215 12 2.0932803823861192 2215 13 1.2896681043859815 2215 14 1.8600140576140376 2215 15 1.163605369630048 2215 16 0.5473697730962925 2215 17 0.6536773128490836 2215 18 1.4082459920654884 2215 19 0.8240782201624555 2215 20 1.679002889989718 2215 21 1.5415408938532522 2215 22 0.8914772491128318 2215 23 1.3873291922950877 2215 24 1.3399927415553954 2215 25 0.8390517288718762 2215 26 1.0049094969268637 2215 27 -0.249906009780819 2215 28 0.32676432701728686 2215 29 -0.12459110809703233 2215 30 2.8283859240482343 2215 31 1.9541918776019962 2215 32 1.9012933460625092 2215 33 1.6671579632512241 2215 34 1.4373673883589753 2215 35 2.5733868890371276 2215 36 2.350324232525491 2215 37 2.6846307501363285 2215 38 0.9783210823427453 2215 39 2.4052246701782636 2215 40 2.892272735986669 2215 41 2.333806305044533 2215 42 2.1479453177262613 2215 43 1.9724478090731274 2215 44 2.5452666573262483 2215 45 2.6829415707014057 2215 46 2.1793242472011407 2215 47 2.5588798878081853 2215 48 2.2778098195076275 2215 49 2.953025935999271 2215 50 2.73103808485023 2216 1 -0.977473391547079 2216 2 -0.1764676180789273 2216 3 -0.38614638098686527 2216 4 -1.4388192336211674 2216 5 -0.403507359478958 2216 6 0.925222736484006 2216 7 -0.8204912011530112 2216 8 -0.30618850466278147 2216 9 -1.7510424167157659 2216 10 -2.173785879671719 2216 11 -1.8690542555430625 2216 12 -0.33628483500518414 2216 13 -0.9265637796719908 2216 14 -0.49187000035697537 2216 15 -0.8097279637032884 2216 16 -2.012630226903706 2216 17 -0.5498009480204828 2216 18 -1.021319225325815 2216 19 -2.1196898957795725 2216 20 -2.2717217476914406 2216 21 -1.9135315699148627 2216 22 -2.1815662291480393 2216 23 -2.0938302279947685 2216 24 -1.2118913164156169 2216 25 -1.3487743580846434 2216 26 -1.6188586190151675 2216 27 -1.2910654300706739 2216 28 -3.390337122258078 2216 29 -2.4353157457781904 2216 30 -1.9461068295749548 2216 31 -1.4730544992095993 2216 32 -0.6245037553867654 2216 33 -1.8319724715313832 2216 34 -1.3365456551192842 2216 35 -0.2718305022672176 2216 36 -1.4560525790687109 2216 37 0.22202205448415313 2216 38 -0.8390702220050805 2216 39 -0.4481086631550717 2216 40 0.3641567939576831 2216 41 -2.094019781911989 2216 42 -2.0039387402447524 2216 43 -2.4113203068689018 2216 44 -2.561979719485347 2216 45 -2.662565675675406 2216 46 -2.5331395209148013 2216 47 -1.9245983730613787 2216 48 -1.7511756877387477 2216 49 -2.4614668176239185 2216 50 -2.0121503209468727 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 0.0 6 13.0 7 215.0 8 1342.0 9 2955.0 10 2934.0 11 2495.0 12 2323.0 13 2223.0 14 2215.0 15 2187.0 16 2068.0 17 1905.0 18 1718.0 19 1559.0 20 1450.0 21 1396.0 22 1300.0 23 1137.0 24 1103.0 25 1025.0 26 947.0 27 961.0 28 870.0 29 820.0 30 754.0 31 756.0 32 688.0 33 664.0 34 633.0 35 762.0 36 848.0 37 1032.0 38 1425.0 39 3141.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.8187751004016 19.256191432396253 10.171937751004016 37.753095716198125 2 21.990764923424084 16.28047888677629 38.872986356323516 22.85576983347611 3 20.82776199231155 18.903560086912922 36.90665218118001 23.36202573959552 4 16.770082523764753 28.08315052752533 26.01274417632926 29.134022772380654 5 17.996448344301683 26.888122845502977 28.25028726626972 26.865141543925624 6 26.87558759009715 25.632508095685786 23.09411887600543 24.397785438211635 7 24.136634283923534 24.498067481458268 17.223440927608898 34.141857307009296 8 21.163689543507783 24.30377102266792 18.403844144991123 36.12869528883318 9 26.568473832654337 16.279118353703122 16.7888854068735 40.363522406769036 10 21.15533270657056 22.855949023294684 28.93972631359031 27.048991956544448 11 30.895226156899614 17.127337302830878 21.481249347122112 30.496187193147396 12 22.776559072391102 20.371879243706257 26.209129844353914 30.642431839548728 13 26.94509443423032 15.34347317399298 24.1246030419522 33.586829349824505 14 34.15999832856277 17.174017508304953 19.41583268913357 29.250151473998702 15 26.009652549986416 22.606188496333285 21.54900445020162 29.835154503478677 16 31.68343758498776 18.418024727516162 20.221334281709588 29.67720340578649 17 38.35032592344977 18.14307203743941 18.339461808457298 25.167140230653516 18 32.613653005292996 16.475240067783844 19.105002196698674 31.806104730224483 19 29.2886591465584 17.420421488814014 21.446958123181883 31.8439612414457 20 33.50364352123293 16.783231426417622 23.056788675768487 26.656336376580953 21 27.231226703522214 19.035054655107427 18.289567366084515 35.44415127528584 22 33.69422092172641 15.934789424182256 17.027902602152786 33.343087051938554 23 39.57123156003176 18.03042333570145 17.99908061348155 24.39926449078524 24 28.665551139928887 26.35013595482117 16.891863626856306 28.092449278393644 25 36.03377644009698 19.258423208761812 16.718919822757293 27.988880528383913 26 33.49912229373903 24.492184234723734 17.228120036780073 24.780573434757166 27 29.196012455850695 24.447741854584212 21.156137014357668 25.20010867520742 28 36.54183733177297 21.691883306862827 17.95745214411101 23.8088272172532 29 30.428875977093174 21.184634034193035 22.98624754420432 25.40024244450947 30 35.83315910516413 19.439682207819363 19.293330545682625 25.43382814133389 31 31.94900198557843 19.258020691817325 22.80280071062807 25.990176611976175 32 29.85411528654433 22.451197592275214 21.878526940601095 25.816160180579356 33 33.56463580311422 18.64980666736336 24.35364196885777 23.431915560664645 34 29.642170387091383 22.293286514505475 22.82626870662988 25.238274391773263 35 30.77598277915944 24.326527200150476 20.5542435578591 24.34324646283099 36 36.21734587251829 18.522466039707417 22.693834900731453 22.566353187042843 37 30.810223401809782 19.803138910367597 23.57944452571524 25.80719316210737 38 32.1343476988672 17.297161727208128 26.330309743761234 24.23818083016344 39 33.583446546138575 17.909917441738948 25.379872504963945 23.126763507158532 40 26.036282982151448 28.522106672804505 24.609236048628404 20.832374296415644 41 30.555091121885976 15.294265173047986 25.794181574987462 28.35646213007858 42 24.12113865317895 18.340091125694936 32.88885173264223 24.649918488483884 43 24.44723092998955 15.304075235109718 29.205851619644722 31.042842215256005 44 25.021418869501623 15.327551979939399 33.21700971685299 26.434019433705984 45 24.47546602022904 15.28462760177213 36.43107916074563 23.8088272172532 46 24.420669912447497 19.282445619240654 28.89023549324028 27.40664897507157 47 28.70337477797513 17.924981715599206 32.1680075227249 21.203635983700764 48 28.369935432643086 21.814990492508933 25.645151179555757 24.169922895292224 49 26.43181010886145 20.341001692471636 26.657472993585323 26.569715205081597 50 27.418815564007186 22.888368788398044 24.144272161156852 25.548543486437914 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.0 8 1.0 9 0.5 10 0.0 11 11.5 12 23.0 13 21.0 14 19.0 15 12.5 16 6.0 17 10.0 18 14.0 19 24.5 20 35.0 21 36.0 22 37.0 23 71.0 24 105.0 25 114.0 26 123.0 27 181.5 28 240.0 29 231.0 30 222.0 31 336.0 32 450.0 33 527.5 34 605.0 35 691.5 36 778.0 37 876.0 38 974.0 39 1116.5 40 1259.0 41 1414.5 42 1570.0 43 1667.0 44 1764.0 45 1886.5 46 2009.0 47 2407.5 48 2806.0 49 2819.0 50 2832.0 51 2998.5 52 3165.0 53 3222.5 54 3280.0 55 3348.0 56 3416.0 57 3215.0 58 3014.0 59 2911.5 60 2809.0 61 2640.0 62 2471.0 63 2415.5 64 2360.0 65 2203.0 66 2046.0 67 1897.5 68 1749.0 69 1656.0 70 1563.0 71 1457.5 72 1352.0 73 1294.5 74 1237.0 75 1103.0 76 969.0 77 906.0 78 843.0 79 711.5 80 580.0 81 503.0 82 426.0 83 350.5 84 275.0 85 229.5 86 184.0 87 146.5 88 109.0 89 83.5 90 58.0 91 51.0 92 44.0 93 31.0 94 18.0 95 13.5 96 9.0 97 7.0 98 5.0 99 7.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11908492635537449 2 0.008356836937219262 3 0.0020892092343048154 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0020892092343048154 14 0.004178418468609631 15 0.004178418468609631 16 0.1337093909955082 17 0.0020892092343048154 18 0.13788780946411783 19 0.1713151572129949 20 0.2277238065392249 21 0.23190222500783453 22 0.041784184686096314 23 0.01462446464013371 24 0.11490650788676486 25 0.04387339392040113 26 0.027159720045962607 27 0.03342734774887705 28 0.027159720045962607 29 0.039694975451791494 30 0.07312232320066855 31 0.041784184686096314 32 0.039694975451791494 33 0.041784184686096314 34 0.04387339392040113 35 0.03342734774887705 36 0.031338138514572234 37 0.02924892928026742 38 0.039694975451791494 39 0.041784184686096314 40 0.15460148333855636 41 0.03551655698318187 42 0.039694975451791494 43 0.031338138514572234 44 0.020892092343048157 45 0.027159720045962607 46 0.016713673874438523 47 0.020892092343048157 48 0.016713673874438523 49 0.012535255405828893 50 0.02298130157735297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 47865.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.60399038963752 #Duplication Level Percentage of deduplicated Percentage of total 1 97.84816539213085 84.74041575263762 2 0.5910308059730297 1.0237125248093595 3 0.16886594456372278 0.43873393920401127 4 0.12061853183123056 0.4178418468609631 5 0.10131956673823367 0.43873393920401127 6 0.09649482546498443 0.5014102162331557 7 0.12303090246785517 0.7458476966468192 8 0.11338141992135672 0.7855426720986106 9 0.1350927556509782 1.052961454089627 >10 0.6971751139845126 9.593648803927714 >50 0.004824741273249222 0.261151154288102 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACG 73 0.15251227410425153 No Hit GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG 52 0.10863888018385043 No Hit CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA 50 0.10446046171524077 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.08356836937219263 0.0 2 0.0 0.0 0.0 0.08565757860649745 0.0 3 0.0 0.0 0.0 0.09819283401232634 0.0 4 0.0 0.0 0.0 0.12326334482398413 0.0 5 0.0 0.0 0.0 0.16922594797869006 0.0 6 0.0 0.0 0.0 0.16922594797869006 0.0 7 0.0 0.0 0.0 0.16922594797869006 0.0 8 0.0 0.0 0.0 0.16922594797869006 0.0 9 0.0 0.0 0.0 0.1713151572129949 0.0020892092343048154 10 0.0 0.0 0.0 0.1713151572129949 0.0020892092343048154 11 0.0 0.0 0.0 0.1734043664472997 0.0020892092343048154 12 0.0 0.0 0.0 0.2005640864932623 0.0020892092343048154 13 0.0 0.0 0.0 0.2005640864932623 0.0020892092343048154 14 0.0 0.0 0.0 0.21101013266478638 0.0020892092343048154 15 0.0020892092343048154 0.0 0.0 0.22563459730492008 0.0020892092343048154 16 0.0020892092343048154 0.0 0.0 0.24443748041366342 0.0020892092343048154 17 0.0020892092343048154 0.0 0.0 0.2736864096939308 0.0020892092343048154 18 0.0020892092343048154 0.0 0.0 0.4136634283923535 0.0020892092343048154 19 0.0020892092343048154 0.0 0.0 0.747936905881124 0.0020892092343048154 20 0.0020892092343048154 0.0 0.0 0.8064347644416588 0.0020892092343048154 21 0.0020892092343048154 0.0 0.0 0.8398621121905359 0.0020892092343048154 22 0.0020892092343048154 0.0 0.0 0.8858247153452419 0.0020892092343048154 23 0.0020892092343048154 0.0 0.0 0.9505902016086911 0.0020892092343048154 24 0.0020892092343048154 0.0 0.0 0.9735715031860441 0.0020892092343048154 25 0.0020892092343048154 0.0 0.0 1.0049096417006163 0.0020892092343048154 26 0.0020892092343048154 0.0 0.0 1.0049096417006163 0.0020892092343048154 27 0.0020892092343048154 0.0 0.0 1.0278909432779693 0.0020892092343048154 28 0.0020892092343048154 0.0 0.0 1.0508722448553223 0.0020892092343048154 29 0.0020892092343048154 0.0 0.0 1.119816149587381 0.0020892092343048154 30 0.0020892092343048154 0.0 0.0 1.1616003342734775 0.0020892092343048154 31 0.0020892092343048154 0.0 0.0 1.2765068421602423 0.0020892092343048154 32 0.0020892092343048154 0.0 0.0 1.383056513109788 0.0020892092343048154 33 0.0020892092343048154 0.0 0.0 1.4415543716703227 0.0020892092343048154 34 0.0020892092343048154 0.0 0.0 1.5919774365402695 0.0020892092343048154 35 0.0020892092343048154 0.0 0.0 1.8656638462342003 0.0020892092343048154 36 0.0020892092343048154 0.0 0.0 2.197848114488666 0.0020892092343048154 37 0.0020892092343048154 0.0 0.0 2.7034367491904314 0.0020892092343048154 38 0.0020892092343048154 0.0 0.0 3.142170688394443 0.0020892092343048154 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGAC 20 7.7553437E-4 44.029316 10 AGTCTGT 20 7.8358245E-4 43.9373 42 AGATAGG 25 0.002298886 35.29738 22 GATAGGA 25 0.002298886 35.29738 23 TATTCAC 25 0.0023467133 35.149845 3 CATCGGA 25 0.0023467133 35.149845 9 GTCCCCC 25 0.0023467133 35.149845 7 GAGGCCC 25 0.0023467133 35.149845 26 GGTGGTC 35 3.1988966E-4 31.383787 44 CTTATTC 50 2.2874628E-6 30.820524 1 CTGACCC 30 0.005672134 29.352879 12 TCGGACG 30 0.005672134 29.352879 11 TTATACC 30 0.005730544 29.291536 2 CATGTGT 30 0.005730544 29.291536 33 AGCCCGT 40 6.991287E-4 27.460814 31 GACCCAA 40 6.991287E-4 27.460814 28 AGACCCA 40 6.991287E-4 27.460814 27 TTTACAC 75 6.815753E-8 26.362381 2 TTACACA 85 7.941708E-9 25.845472 3 ACGAGAC 60 9.297562E-6 25.737673 24 >>END_MODULE