FastQCFastQC Report
Wed 25 May 2016
SRR1780605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47865
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2499252.21351718374595No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT499710.439778543821165TruSeq Adapter, Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG32396.766948709913298No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC30586.3888018385041265No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC7911.6525645043351092No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT6201.2953097252689858No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC4030.8419513214248407No Hit
ATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTTCTGCTT2350.4909641700616317RNA PCR Primer, Index 22 (96% over 28bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC2310.48260733312441245No Hit
ACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTTCT1980.4136634283923535RNA PCR Primer, Index 9 (95% over 23bp)
CATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTTCTGCT1620.3384518959573801TruSeq Adapter, Index 9 (96% over 26bp)
CTTATTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG1070.22354538807061528No Hit
CGTCAGATGTGTATAAGACTGTCTCTTATACACATCTCCGAGCCCACGAG750.15669069257286117No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG740.15460148333855636No Hit
TCTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT720.15042306486994672No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC720.15042306486994672No Hit
TCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTTCTGCTTG680.14206622793272747TruSeq Adapter, Index 9 (96% over 28bp)
TCTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC570.11908492635537449No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATATCGTATGCCGT560.11699571712106968No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG540.11281729865246005No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG520.10863888018385043No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG490.10237125248093595No Hit
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA480.10028204324663115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA501.2914825E-939.61
CTTTACA850.038.823531
TTTACAC800.038.52
TCTTTAT357.1450213E-637.7142871
CCGTCTT5350.035.36448744
TTATTAC250.002330354435.22
ATTACAC250.002330354435.24
CTTATTA250.002330354435.21
TATTACA250.002330354435.23
ATGCCGT33100.034.42900544
CTTATAC34150.033.4348451
CTTTATA401.7839924E-533.02
TTTATAC401.7839924E-533.03
TTATACA34550.032.984082
GCCGTCT5800.032.6206943
TATACAC35000.032.4971433
TCTGCTT551.3274985E-732.044
TATGCCG37750.030.12979943
TTACACA1050.029.3333323
ATACACA39450.028.88724
Produced by FastQC (version 0.11.5)