FastQCFastQC Report
Wed 25 May 2016
SRR1780604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75474
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCCA150.002171196370.367751
CCGGCAC150.002171196370.367751
CGCTGGG150.002217500269.99477
TTAAGGC150.002217500269.994730
TGGGCAT150.002217500269.994761
CCTTCAC252.377587E-455.99575832
AAGCGAG252.377587E-455.9957589
ACTTAGC200.00678481152.7758181
GACAGCG200.006929088452.49602537
TGCCCCA200.006929088452.49602560
CGTGCGA200.006929088452.49602510
TCATTCG200.006929088452.49602543
CCAATGC200.006929088452.4960253
AGGACGA200.006929088452.49602554
GCCGGGC200.006929088452.49602526
AAACGTA200.006929088452.49602515
ACGATGG200.006929088452.49602557
AACGAGG200.006929088452.49602566
GAAAACG200.006929088452.49602513
GGACGAT200.006929088452.49602555
Produced by FastQC (version 0.11.5)