FastQCFastQC Report
Wed 25 May 2016
SRR1780604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75474
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT6010.7963007128282586No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT3360.4451864218141347RNA PCR Primer, Index 30 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC810.10732172668733604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA308.304767E-658.33333225
TGAAGGT252.376697E-455.99999628
TGGCGTG252.376697E-455.99999623
AGTACTA252.376697E-455.9999965
GACAGGG200.00692701552.52
ACAGTTC200.00692701552.58
TCGTTCA200.00692701552.520
TACCCGC200.00692701552.511
ATAGGCG200.00692701552.53
GCAACTG200.00692701552.529
GGCAACT200.00692701552.528
CACGCTA200.00692701552.523
CGTTCAT200.00692701552.521
GACCATG200.00692701552.57
CGGGAGT200.00692701552.51
GGAGTAC200.00692701552.53
CTACCCG200.00692701552.510
GGGCAAA200.00692701552.59
CCACGCT200.00692701552.522
ATGATTC305.844353E-446.66666440
Produced by FastQC (version 0.11.5)