##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780604_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 75474 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74464053846358 34.0 34.0 34.0 31.0 34.0 2 33.186448313326444 34.0 34.0 34.0 32.0 34.0 3 33.40857778837746 34.0 34.0 34.0 33.0 34.0 4 33.4666507671516 34.0 34.0 34.0 34.0 34.0 5 33.48384874261335 34.0 34.0 34.0 34.0 34.0 6 37.14235365821342 38.0 37.0 38.0 36.0 38.0 7 36.81784455574105 38.0 37.0 38.0 34.0 38.0 8 36.79961311180009 38.0 37.0 38.0 34.0 38.0 9 36.58848080133556 38.0 37.0 38.0 34.0 38.0 10-11 36.296062220102286 38.0 37.0 38.0 33.0 38.0 12-13 36.18814426160002 38.0 37.0 38.0 33.5 38.0 14-15 36.462437395659435 38.0 37.0 38.0 34.0 38.0 16-17 36.61706017966452 38.0 37.0 38.0 34.0 38.0 18-19 36.623824098365 38.0 37.0 38.0 34.0 38.0 20-21 36.356202135834856 38.0 37.0 38.0 34.0 38.0 22-23 36.40654397540875 38.0 37.0 38.0 34.0 38.0 24-25 35.72540212523518 38.0 36.5 38.0 31.5 38.0 26-27 35.955713225746614 38.0 37.0 38.0 33.0 38.0 28-29 35.83755332962345 38.0 36.5 38.0 31.5 38.0 30-31 35.538476826456794 38.0 36.0 38.0 31.0 38.0 32-33 36.02883112065082 38.0 37.0 38.0 33.0 38.0 34-35 36.08037867345046 38.0 37.0 38.0 33.0 38.0 36-37 36.21664414235366 38.0 37.0 38.0 34.0 38.0 38-39 35.86262818984021 38.0 36.5 38.0 32.5 38.0 40-41 35.37171078782097 38.0 36.0 38.0 30.5 38.0 42-43 35.77647269258288 38.0 36.5 38.0 32.5 38.0 44-45 35.97600498184805 38.0 37.0 38.0 33.0 38.0 46-47 36.2829848689615 38.0 37.0 38.0 34.0 38.0 48-49 36.05092482179293 38.0 37.0 38.0 33.0 38.0 50-51 36.21207303177253 38.0 37.0 38.0 34.0 38.0 52-53 36.141876672761484 38.0 37.0 38.0 34.0 38.0 54-55 36.32865622598511 38.0 37.0 38.0 34.0 38.0 56-57 36.38033627474363 38.0 37.0 38.0 34.0 38.0 58-59 36.431526088454305 38.0 37.0 38.0 34.0 38.0 60-61 36.09479423377587 38.0 37.0 38.0 33.0 38.0 62-63 36.38619259612582 38.0 37.0 38.0 34.0 38.0 64-65 36.38749768132072 38.0 37.0 38.0 34.0 38.0 66-67 35.781341919071465 38.0 36.5 38.0 31.5 38.0 68-69 35.22269920767417 38.0 36.0 38.0 29.0 38.0 70-71 35.270516999231525 38.0 36.0 38.0 30.0 38.0 72-73 35.004173622704506 38.0 36.0 38.0 27.5 38.0 74-75 35.681380342899544 38.0 36.5 38.0 31.5 38.0 76 35.084254180247505 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2661811540289065 1101 2 0.2086456131048493 1101 3 0.2506262425273462 1101 4 0.25878953121828374 1101 5 0.3650006257053633 1101 6 -1.9640383541830602 1101 7 0.040667244221474164 1101 8 0.6378379343644696 1101 9 0.28497662311630023 1101 10-11 0.4877351800582588 1101 12-13 0.7947163626386669 1101 14-15 0.6300479841816085 1101 16-17 0.1988111160223056 1101 18-19 -0.18304352278908453 1101 20-21 -0.05902368093153143 1101 22-23 0.6694430054736955 1101 24-25 -1.0158956756429731 1101 26-27 -0.2775504877934267 1101 28-29 0.437904311192014 1101 30-31 0.5410174291475727 1101 32-33 -0.23873713634957028 1101 34-35 0.6620107034345608 1101 36-37 0.269749483224615 1101 38-39 0.20448041468373646 1101 40-41 1.4040015344436085 1101 42-43 0.8387042587906706 1101 44-45 0.9248380579641591 1101 46-47 0.5964076467169193 1101 48-49 -1.3152923768673617 1101 50-51 -1.1910816436833827 1101 52-53 -1.158165830124716 1101 54-55 -0.7508045506784455 1101 56-57 -1.0638896329192704 1101 58-59 -0.9956871900594493 1101 60-61 -1.2015997388127886 1101 62-63 -0.8042345213231457 1101 64-65 -0.9515855226659156 1101 66-67 -1.1738139088182038 1101 68-69 -1.2846262521883105 1101 70-71 -1.276541514071802 1101 72-73 -1.836986021102092 1101 74-75 -0.8290480847432988 1101 76 -1.829618124109004 1102 1 -0.8712387177659693 1102 2 0.0471872797715136 1102 3 -0.22012696260085818 1102 4 0.28883760814136394 1102 5 -0.03243527173053451 1102 6 0.30118600479129753 1102 7 0.46454544934967856 1102 8 0.5396808830824185 1102 9 0.6010823923470667 1102 10-11 0.639377808263383 1102 12-13 0.6042115549463603 1102 14-15 -0.37716355427992454 1102 16-17 -0.1589613198751323 1102 18-19 -0.48432557407114274 1102 20-21 0.22583208829923507 1102 22-23 0.6752523003454911 1102 24-25 -3.9125302910275863 1102 26-27 -1.2847620262549668 1102 28-29 -0.023634150346445892 1102 30-31 1.4935414676091128 1102 32-33 0.5407099790350429 1102 34-35 0.6533969213832762 1102 36-37 -2.927165580877947 1102 38-39 -0.36503080326497894 1102 40-41 0.894786790853864 1102 42-43 0.5911081049445102 1102 44-45 0.3378989554000569 1102 46-47 0.5651576467169193 1102 48-49 -3.045060005072486 1102 50-51 -0.6946874129141563 1102 52-53 -0.13613057371446047 1102 54-55 0.19891500060360556 1102 56-57 0.552496585029445 1102 58-59 0.21785447660721502 1102 60-61 -5.773915443941 1102 62-63 -2.4632890085026347 1102 64-65 -0.18075218933257986 1102 66-67 0.9419713475920517 1102 68-69 -0.6225669573165078 1102 70-71 -1.1401232448410283 1102 72-73 -6.20257095699953 1102 74-75 -2.540987187307394 1102 76 -0.5888328676987484 1103 1 1.0306277980711371 1103 2 0.5700945195905192 1103 3 -0.2107943834153332 1103 4 0.40224258551692316 1103 5 0.22576608573552903 1103 6 0.22991903646550327 1103 7 0.9074187525170956 1103 8 0.8250315618154502 1103 9 0.5600756050167561 1103 10-11 0.4258597087158762 1103 12-13 -0.4908110694880321 1103 14-15 0.017774228525503588 1103 16-17 0.6237027072741839 1103 18-19 0.924328272082704 1103 20-21 0.3758603688422255 1103 22-23 0.8348959655038684 1103 24-25 -2.5608617389913846 1103 26-27 0.12417462532874168 1103 28-29 0.28024028404269075 1103 30-31 0.9533830965683876 1103 32-33 1.2068581690802915 1103 34-35 0.6259647946864391 1103 36-37 -0.7827934089322497 1103 38-39 0.09085154967620213 1103 40-41 -0.31901411412351166 1103 42-43 -0.26494393125457805 1103 44-45 1.3111738422778814 1103 46-47 0.9951633028255173 1103 48-49 -2.166242131769323 1103 50-51 -0.05370890612682189 1103 52-53 0.3058943131633711 1103 54-55 0.10968988748143005 1103 56-57 0.6724060872918898 1103 58-59 -0.07499054601721866 1103 60-61 -4.35522200502697 1103 62-63 -1.2429835786383734 1103 64-65 0.6267987156447958 1103 66-67 0.513238315917846 1103 68-69 0.675509965760412 1103 70-71 0.5627896510865682 1103 72-73 -5.1447372465922925 1103 74-75 -2.206569766492919 1103 76 -3.0300093382869804 1104 1 0.13383240973666943 1104 2 0.5583174167578164 1104 3 0.352295763075297 1104 4 -0.2838046468639064 1104 5 -0.32021626927180336 1104 6 0.20134406590825904 1104 7 0.5933213538105164 1104 8 0.5883944412137865 1104 9 0.7202648651254293 1104 10-11 -0.6021264066997318 1104 12-13 0.3434107119537373 1104 14-15 0.5606986691131155 1104 16-17 0.3352979881684206 1104 18-19 0.7002327329186713 1104 20-21 -0.0064409183744515985 1104 22-23 0.6633428342408223 1104 24-25 0.2920381143113602 1104 26-27 0.9894301058138737 1104 28-29 1.3013460920139295 1104 30-31 1.1645548149923215 1104 32-33 0.8957876924175565 1104 34-35 0.5224915824299927 1104 36-37 1.2891915494346549 1104 38-39 1.3129635767841918 1104 40-41 2.034495255904794 1104 42-43 0.7503766332198936 1104 44-45 0.9206285830783116 1104 46-47 0.9405857289086939 1104 48-49 0.16547959801848577 1104 50-51 0.5704052284627394 1104 52-53 0.56550711508077 1104 54-55 0.5858464196411859 1104 56-57 0.4761003784962554 1104 58-59 -0.7389092220229188 1104 60-61 0.685636277168939 1104 62-63 0.4979201590421596 1104 64-65 0.834230687379744 1104 66-67 -0.005078178224593444 1104 68-69 1.8434059966701426 1104 70-71 1.4966105978003483 1104 72-73 1.0733664332358046 1104 74-75 0.15447075087314488 1104 76 0.010921259452643994 1105 1 0.7715730409598862 1105 2 0.48964322690807904 1105 3 0.16387422343777303 1105 4 -0.14959427935440317 1105 5 0.04827889295940224 1105 6 0.21570650631620225 1105 7 0.7195861135313137 1105 8 -0.4680495472801667 1105 9 0.33432959668458295 1105 10-11 0.2050970559760259 1105 12-13 -0.4757613355992163 1105 14-15 0.19970657448997287 1105 16-17 0.09719125552303609 1105 18-19 0.6896620572402767 1105 20-21 0.13873237633718816 1105 22-23 0.10454533660438159 1105 24-25 0.5917585093790549 1105 26-27 0.16614444612727652 1105 28-29 0.7543966019409112 1105 30-31 1.0338244225396451 1105 32-33 0.8296480508750932 1105 34-35 -0.7353484191792461 1105 36-37 0.3700959406401765 1105 38-39 0.2585713654913775 1105 40-41 0.3010050219619629 1105 42-43 0.25679346583912377 1105 44-45 0.6926198127365524 1105 46-47 0.8645794528843211 1105 48-49 -0.31576273296133905 1105 50-51 0.39544093340912667 1105 52-53 0.4487770847064141 1105 54-55 0.46284036488690106 1105 56-57 0.3066477202378479 1105 58-59 0.28049214180545334 1105 60-61 0.8547167993663578 1105 62-63 -0.18031289870933875 1105 64-65 0.5528656520771165 1105 66-67 0.4186741601979449 1105 68-69 0.18516770889833367 1105 70-71 1.073713166544941 1105 72-73 1.2152005611299188 1105 74-75 -0.3418280039797068 1105 76 1.1863280946868855 1106 1 -1.1597002562275058 1106 2 0.1529565105407471 1106 3 -0.0037808087547048785 1106 4 -0.04289316108940966 1106 5 -0.0997429640382208 1106 6 0.18099369709899094 1106 7 -1.0643007044964747 1106 8 0.34737319077472506 1106 9 -0.13449453072985307 1106 10-11 0.31005088518645607 1106 12-13 1.1691154011001998 1106 14-15 -0.10793278504916515 1106 16-17 0.1319040647402474 1106 18-19 0.5589436566980908 1106 20-21 -0.16118714246999133 1106 22-23 0.386790761883951 1106 24-25 -0.3596456756429731 1106 26-27 0.6984110506681134 1106 28-29 0.5773273881150942 1106 30-31 0.5945030060706529 1106 32-33 -0.20688617481110327 1106 34-35 0.6726276906140427 1106 36-37 0.8757190345066661 1106 38-39 0.35131535058118146 1106 40-41 0.9092098677769442 1106 42-43 0.7545696434060574 1106 44-45 0.7970335707846701 1106 46-47 0.8536191851784594 1106 48-49 1.2193630718505872 1106 50-51 -0.32449510522184255 1106 52-53 0.34944634936245933 1106 54-55 0.03785730829591216 1106 56-57 0.2568235081063648 1106 58-59 0.5423737073764414 1106 60-61 0.8943537868282334 1106 62-63 0.5775763761127592 1106 64-65 -0.9908483431787332 1106 66-67 0.1078367322074385 1106 68-69 1.4928176580681054 1106 70-71 0.13631906285127826 1106 72-73 0.18925596607738981 1106 74-75 -0.3775256488458538 1106 76 0.6996286707627917 1107 1 0.7005748386327895 1107 2 0.11529266093743473 1107 3 0.3034803157709973 1107 4 0.14324861193577476 1107 5 0.06688777069788898 1107 6 0.20416979230423493 1107 7 0.691218440036117 1107 8 0.08881420491753289 1107 9 -0.45822685222002235 1107 10-11 0.4152272391016041 1107 12-13 -0.41769254991739047 1107 14-15 0.24243587655828946 1107 16-17 0.599759359669541 1107 18-19 0.725121649109262 1107 20-21 0.7961472831940313 1107 22-23 0.4674974710940276 1107 24-25 0.2248554454332563 1107 26-27 0.018834041354558906 1107 28-29 0.8222933246449315 1107 30-31 0.4702144582955583 1107 32-33 0.9024834353993043 1107 34-35 -0.20291128834421812 1107 36-37 1.0725218113262613 1107 38-39 0.6193603661037699 1107 40-41 0.4436829619473457 1107 42-43 0.7536425995978462 1107 44-45 0.5243102179043646 1107 46-47 0.4747708754164748 1107 48-49 0.8174033443583468 1107 50-51 0.9096050155094417 1107 52-53 0.49936873639247636 1107 54-55 0.6263792124007779 1107 56-57 0.9167601241808754 1107 58-59 0.6667053734682042 1107 60-61 1.1965808478837658 1107 62-63 0.7379226161955401 1107 64-65 1.057616644748137 1107 66-67 -0.09433427503643799 1107 68-69 0.9590560161226023 1107 70-71 0.002221098035825264 1107 72-73 1.226273040930785 1107 74-75 -0.4490265974488281 1107 76 1.1136507473409267 1108 1 -0.5057031882730385 1108 2 0.2812119434486462 1108 3 -0.08216990844424998 1108 4 0.3809167733707497 1108 5 0.22832274827636923 1108 6 -0.13215307765092632 1108 7 0.08135296918410262 1108 8 -0.4896611314053345 1108 9 -0.35957214369984314 1108 10-11 0.3389940552451023 1108 12-13 1.2078679881992045 1108 14-15 0.5114941725224114 1108 16-17 -0.19844691835736938 1108 18-19 0.3349871199612835 1108 20-21 0.8522010948532284 1108 22-23 -0.043713291237814644 1108 24-25 0.8930168804108334 1108 26-27 -0.9542601290490325 1108 28-29 -0.9019219219918426 1108 30-31 0.40519203677089166 1108 32-33 0.7388063053544656 1108 34-35 0.7769093394584701 1108 36-37 1.2451675512365696 1108 38-39 1.2538895140858344 1108 40-41 0.898839912619998 1108 42-43 -0.6633977591465481 1108 44-45 0.7014402627474112 1108 46-47 0.748313476313335 1108 48-49 0.5819773353897446 1108 50-51 0.8154346119219937 1108 52-53 -0.11498103939228344 1108 54-55 -0.39604231226290665 1108 56-57 0.4459081062436496 1108 58-59 0.4514587366968996 1108 60-61 0.8131727761348913 1108 62-63 0.7760392081237981 1108 64-65 0.27734758645217283 1108 66-67 0.7038720029904724 1108 68-69 1.2617465990822438 1108 70-71 -0.746657825730999 1108 72-73 -0.38359242992122944 1108 74-75 -1.2091162835474805 1108 76 0.23921128545751458 1109 1 -1.139166068193319 1109 2 0.2596872797715122 1109 3 0.13750124252734963 1109 4 0.09330342010716919 1109 5 0.22194507014980758 1109 6 -0.42556613196084214 1109 7 -0.22780497800074784 1109 8 0.2056851565866964 1109 9 0.6615738453385234 1109 10-11 0.2692282356138094 1109 12-13 0.4963483070831103 1109 14-15 0.7297702064038347 1109 16-17 0.8451305604667496 1109 18-19 0.5405675883220269 1109 20-21 1.0067402079573569 1109 22-23 0.32663050547369465 1109 24-25 -0.09895123119853366 1109 26-27 0.34869951220657214 1109 28-29 -0.8364012443635431 1109 30-31 0.675635484703129 1109 32-33 0.6679642525393206 1109 34-35 0.1451704256567794 1109 36-37 1.030409205446837 1109 38-39 0.7918068035726336 1109 40-41 0.6928209788880579 1109 42-43 0.9518987032351163 1109 44-45 0.8625463912974851 1109 46-47 0.6680743133835847 1109 48-49 0.8680062342437509 1109 50-51 0.3380155785388368 1109 52-53 -0.34170749679137913 1109 54-55 -0.597228161789559 1109 56-57 0.07677008930294704 1109 58-59 0.09743780994054418 1109 60-61 1.0874627611872114 1109 62-63 0.4058349231212972 1109 64-65 0.9918866995563107 1109 66-67 0.6666722022929079 1109 68-69 0.006450136700578923 1109 70-71 0.3205070970393038 1109 72-73 0.3862431455645705 1109 74-75 -0.12050641807662998 1109 76 0.4280902092243295 1110 1 -0.6074864816300121 1110 2 -0.10310341790290778 1110 3 0.26804387818626196 1110 4 0.25464708935781744 1110 5 0.3852525636898605 1110 6 0.18911087062313925 1110 7 0.6359676318183745 1110 8 0.7298195235117575 1110 9 0.914700460325605 1110 10-11 -0.45966065327507977 1110 12-13 0.6979503742665756 1110 14-15 0.8396410074374216 1110 16-17 0.7191357284253996 1110 18-19 0.04185957798610218 1110 20-21 1.1494792260452158 1110 22-23 -0.6102687193325025 1110 24-25 -0.25455846634064727 1110 26-27 1.0356762563926196 1110 28-29 0.5958984972385224 1110 30-31 0.33821946403129033 1110 32-33 0.6097730380690365 1110 34-35 0.993490839093468 1110 36-37 1.2418303940773256 1110 38-39 1.0277241162341326 1110 40-41 -1.0922436205951485 1110 42-43 -0.12975504353491374 1110 44-45 0.7875076316075678 1110 46-47 0.6958262513680822 1110 48-49 1.3125540378613252 1110 50-51 -0.023276411125245033 1110 52-53 0.33178087530163936 1110 54-55 -0.2631713142443388 1110 56-57 0.61439282832103 1110 58-59 -0.33868622106720636 1110 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-0.8300209247116541 2116 48-49 0.010228456465974034 2116 50-51 -0.9899209293976696 2116 52-53 0.44400678892290557 2116 54-55 -0.43373609829749427 2116 56-57 -0.2064045138716537 2116 58-59 -2.40446695196421 2116 60-61 -1.3620610483365994 2116 62-63 0.5080571453435212 2116 64-65 -1.62557361790401 2116 66-67 -0.9653912897705865 2116 68-69 -1.2405339902835522 2116 70-71 0.8608245573567714 2116 72-73 -0.24994733062590768 2116 74-75 -0.22336356093376963 2116 76 -1.326195505061385 2117 1 0.8490410025137507 2117 2 0.7346872797715136 2117 3 0.35810730313340855 2117 4 0.12668725849101037 2117 5 0.1074248681296055 2117 6 0.45546922157451064 2117 7 0.891993001797232 2117 8 0.6970235404250786 2117 9 -1.1056483768836998 2117 10-11 -0.4017061078205302 2117 12-13 -1.2670734100886065 2117 14-15 -0.06597474309111817 2117 16-17 -1.057344187008006 2117 18-19 -1.880960189455756 2117 20-21 -0.10305777184062492 2117 22-23 -0.04480888846569542 2117 24-25 -2.3397593120066134 2117 26-27 -1.8457323059752468 2117 28-29 -1.3518684160807126 2117 30-31 -0.5903746163069741 2117 32-33 -2.5434246363495703 2117 34-35 0.12105426404061603 2117 36-37 -1.2901463501087207 2117 38-39 0.38606185407768123 2117 40-41 -0.3606764958594155 2117 42-43 0.27620425879067056 2117 44-45 -0.14855209355098964 2117 46-47 -0.8126832623739872 2117 48-49 -0.5068169980794792 2117 50-51 -0.6782975527742892 2117 52-53 0.5686334122995262 2117 54-55 -0.7709276567390546 2117 56-57 0.07129029132315168 2117 58-59 -1.1741909779382382 2117 60-61 -0.604772087297647 2117 62-63 -0.5338746728382944 2117 64-65 -1.0074567347871266 2117 66-67 -0.6259919391212279 2117 68-69 0.04373549023593171 2117 70-71 -1.1252631049808954 2117 72-73 0.4047279940494235 2117 74-75 0.6742663091960992 2117 76 0.5108174819516051 2118 1 -0.32260276069978033 2118 2 0.3655012332598844 2118 3 -0.758700307860245 2118 4 0.11162383354386662 2118 5 0.3247874474107917 2118 6 0.2600411031812797 2118 7 0.7545722829811652 2118 8 0.4635404537443151 2118 9 -1.6318111675813753 2118 10-11 -0.5009397230425208 2118 12-13 -1.0968170675938893 2118 14-15 0.33789682139091326 2118 16-17 -3.339585201807161 2118 18-19 -0.3947683289906436 2118 20-21 -0.44238123907106797 2118 22-23 0.31763825741168006 2118 24-25 0.7588136266826098 2118 26-27 -3.0951376970957583 2118 28-29 -0.9320084795056616 2118 30-31 -0.9460828615501029 2118 32-33 -0.14952928751236527 2118 34-35 -0.5611603236972229 2118 36-37 -3.653518443131979 2118 38-39 -0.7304154186495921 2118 40-41 -0.17073199268816808 2118 42-43 0.45280309599996826 2118 44-45 -2.854352833508713 2118 46-47 -0.13033653932959766 2118 48-49 -0.38512038074333077 2118 50-51 -0.9930438529857071 2118 52-53 -1.1391493572564926 2118 54-55 -0.9364271281978276 2118 56-57 0.02253236320475338 2118 58-59 -0.6404304071137119 2118 60-61 -1.20420390547946 2118 62-63 -1.3864777383774012 2118 64-65 0.01474199671392995 2118 66-67 0.13226651753839036 2118 68-69 -1.636431000250326 2118 70-71 -0.7894412233741264 2118 72-73 0.653142370370773 2118 74-75 -0.4992273483091836 2118 76 -0.8781888605431121 2119 1 0.4338044533486354 2119 2 -1.5348705433577372 2119 3 -0.8657640635951012 2119 4 -0.4619800266048486 2119 5 -0.3667170613694637 2119 6 -1.1275162453395282 2119 7 -0.8961950006764852 2119 8 0.2504017098746729 2119 9 -0.12010415919662165 2119 10-11 -0.2834701770846024 2119 12-13 -0.6595247087899025 2119 14-15 -0.5072819477911779 2119 16-17 -0.535776469011715 2119 18-19 -0.6358496452380678 2119 20-21 -1.8573867761696263 2119 22-23 -0.20996813398208047 2119 24-25 0.3442233719760779 2119 26-27 0.31237298159432925 2119 28-29 -0.7570361649984605 2119 30-31 1.022256799895871 2119 32-33 0.3728168602490669 2119 34-35 0.18757405377469638 2119 36-37 0.06614616689807917 2119 38-39 -2.189599092118975 2119 40-41 -2.0197867308625135 2119 42-43 -0.6041103670596684 2119 44-45 0.0010497926580370631 2119 46-47 -0.17995289750076893 2119 48-49 -0.8877307272074901 2119 50-51 0.10409267604450889 2119 52-53 -0.2781700818253938 2119 54-55 -0.3134639894539646 2119 56-57 -1.6348398880213182 2119 58-59 -0.508548584617273 2119 60-61 -0.8445100279284361 2119 62-63 -0.46718094989456915 2119 64-65 -0.3165940260672713 2119 66-67 -0.510017140110719 2119 68-69 0.09701703012461849 2119 70-71 -1.1803999324391512 2119 72-73 0.2076036897822604 2119 74-75 -0.6826832888249257 2119 76 0.06292014119711808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 3.0 22 9.0 23 12.0 24 27.0 25 74.0 26 134.0 27 247.0 28 360.0 29 696.0 30 1067.0 31 1809.0 32 2759.0 33 4277.0 34 6633.0 35 10226.0 36 18132.0 37 29008.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.19461006439304 21.922781355168667 11.382727826801283 28.499880753637015 2 19.058218724328906 18.856824866841563 36.372790629885785 25.712165778943742 3 17.863105175292155 23.90889577867875 29.320030739062457 28.907968306966637 4 13.886901449505789 17.523915520576622 37.47515700771126 31.11402602220632 5 13.13167448392824 36.27871849908578 36.493361952460454 14.096245064525531 6 29.959986220420276 38.013090600736675 16.858785807032888 15.16813737181016 7 29.055038821315947 29.085512891856798 22.072501788695444 19.786946498131808 8 24.42827993746191 34.99085777883775 19.900893022762805 20.679969260937543 9 23.426610488380103 15.952513448339825 21.509393963484115 39.11148209979596 10-11 24.884728515780267 25.420012189628217 27.278268012825606 22.416991281765906 12-13 24.658160426107003 23.3736121048308 26.690648435222723 25.27757903383947 14-15 21.55113019052919 25.031136550335216 23.630654265044917 29.78707899409068 16-17 22.3149693934335 28.057344251000345 25.91090971725362 23.71677663831253 18-19 22.201685348596868 27.722791954845377 26.669449081803005 23.40607361475475 20-21 21.471632615205234 27.21599491215518 26.472692582876224 24.83967988976336 22-23 22.582611230357475 27.441238042239714 27.1802210032594 22.795929724143413 24-25 22.28317036330392 27.847338156186236 26.97153986803402 22.89795161247582 26-27 21.529930837109468 28.615152237856744 26.904629408803032 22.950287516230755 28-29 21.953255425709518 27.211357553594617 28.1878527704905 22.64753425020537 30-31 21.73331213397991 26.5674271934706 28.06396904894401 23.635291623605482 32-33 22.208972626334898 27.08747383204812 27.22990698783687 23.47364655378011 34-35 22.807854360442008 27.05964968068474 27.286880250152368 22.845615708720885 36-37 23.088745793253306 26.93907835811008 26.96690250947346 23.005273339163153 38-39 21.569017144977078 27.208707634417152 27.628057344251 23.59421787635477 40-41 21.321249701884092 25.97318281792405 29.04377666481172 23.661790815380133 42-43 22.021490844529243 25.616768688555 28.882794074780723 23.47894639213504 44-45 20.599809205819223 25.730052733391634 29.374354082200494 24.295783978588652 46-47 21.79492275485598 24.87015396030421 29.15507327026526 24.179850014574555 48-49 20.502424676047383 25.125208681135224 30.06797042690198 24.304396215915414 50-51 19.84656967962477 25.483610249887377 29.835440019079417 24.834380051408434 52-53 19.302673768450063 26.080504544611387 30.736412539417547 23.880409147521 54-55 18.40170124811193 26.190476190476193 30.32898746588229 25.078835095529588 56-57 19.374884066035985 26.16397699870154 29.268357315101888 25.192781620160588 58-59 18.717704110024645 26.491904496912845 29.908975276254075 24.88141611680844 60-61 19.039669290086653 27.270318255293212 28.529029864589127 25.160982590031004 62-63 18.42886291968095 27.934785489042586 27.630707263428466 26.005644327848 64-65 18.94758459866974 28.652251106341257 26.292498078808595 26.10766621618041 66-67 19.039669290086653 28.142804144473594 25.821474945014177 26.996051620425575 68-69 18.83430055383311 28.13949174550176 25.84731165699446 27.17889604367067 70-71 19.468956196835997 27.962609640405965 25.530646315287385 27.03778784747065 72-73 19.24040066777963 27.738691469910165 25.466385775233856 27.554522087076343 74-75 19.514667302647272 27.699605162042555 25.430611866338076 27.355115668972097 76 19.65842541802475 27.964597079789066 25.52799639610992 26.848981106076263 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 2.0 17 3.0 18 5.5 19 8.0 20 15.5 21 23.0 22 30.5 23 40.5 24 72.0 25 101.0 26 139.0 27 224.0 28 327.0 29 383.0 30 466.5 31 623.0 32 915.5 33 1135.0 34 1285.5 35 1650.0 36 1959.5 37 2055.0 38 2460.0 39 3182.0 40 3896.5 41 4556.0 42 4818.0 43 5036.0 44 5560.5 45 5890.0 46 5913.0 47 5887.0 48 5606.5 49 4970.5 50 4589.0 51 4276.5 52 3590.5 53 2948.0 54 2679.0 55 2502.0 56 2038.0 57 1514.5 58 1278.0 59 1104.5 60 796.5 61 559.0 62 456.0 63 407.0 64 332.0 65 241.5 66 159.5 67 142.0 68 101.0 69 50.0 70 26.0 71 12.0 72 8.5 73 3.0 74 1.5 75 2.0 76 1.5 77 1.0 78 1.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 75474.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.939979330630415 #Duplication Level Percentage of deduplicated Percentage of total 1 61.975941977710946 27.8519755147468 2 16.08880240580223 14.460608951426982 3 8.037030485288048 10.835519516654742 4 4.263223067397842 7.6635662612290325 5 2.7566483872869862 6.194186077324641 6 1.7100064862314994 4.610859368789252 7 1.1675216699097823 3.672787979966611 8 0.7842443540303085 2.819514004822853 9 0.5985022701810249 2.4207011686143574 >10 2.5885960257090628 17.57426398494846 >50 0.023586296361813787 0.6545300368338766 >100 0.0029482870452267234 0.4451864218141347 >500 0.0029482870452267234 0.7963007128282586 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 601 0.7963007128282586 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 336 0.4451864218141347 RNA PCR Primer, Index 30 (95% over 22bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 81 0.10732172668733604 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013249595887325437 0.0 2 0.0 0.0 0.0 0.015899515064790523 0.0 3 0.0 0.0 0.0 0.015899515064790523 0.0 4 0.0 0.0 0.0 0.015899515064790523 0.0 5 0.0 0.0 0.0 0.015899515064790523 0.0 6 0.0 0.0 0.0 0.015899515064790523 0.0 7 0.0 0.0 0.0 0.01722447465352307 0.0 8 0.0 0.0 0.0 0.01722447465352307 0.0 9 0.0 0.0 0.0 0.031799030129581046 0.0 10 0.0 0.0 0.0 0.03444894930704614 0.0 11 0.0 0.0 0.0 0.03577390889577868 0.0 12 0.0 0.0 0.0 0.03709886848451122 0.0 13 0.0 0.0 0.0 0.0423987068394414 0.0 14 0.0 0.0 0.0 0.045048626016906484 0.0 15 0.0 0.0 0.0 0.04769854519437157 0.0 16 0.0 0.0 0.0 0.04769854519437157 0.0 17 0.0 0.0 0.0 0.04902350478310412 0.0 18 0.0 0.0 0.0 0.05432334313803429 0.0 19 0.0 0.0 0.0 0.05829822190423192 0.0 20 0.0 0.0 0.0 0.059623181492964465 0.0 21 0.0 0.0 0.0 0.06227310067042955 0.0 22 0.0 0.0 0.0 0.06624797943662718 0.0 23 0.0 0.0 0.0 0.06757293902535973 0.0 24 0.0 0.0 0.0 0.06889789861409228 0.0 25 0.0 0.0 0.0 0.07949757532395262 0.0 26 0.0 0.0 0.0 0.08877229244508042 0.0 27 0.0 0.0 0.0 0.11129660545353366 0.0 28 0.0 0.0 0.0 0.13249595887325435 0.0 29 0.0 0.0 0.0 0.15899515064790523 0.0 30 0.0 0.0 0.0 0.18946922118875376 0.0 31 0.0 0.0 0.0 0.2159684129634046 0.0 32 0.0 0.0 0.0 0.2742666348676365 0.0 33 0.0 0.0 0.0 0.3458144526591939 0.0 34 0.0 0.0 0.0 0.4001377957972282 0.0 35 0.0 0.0 0.0 0.48758512865357606 0.0 36 0.0 0.0 0.0 0.6624797943662718 0.0 37 0.0 0.0 0.0 0.987094893605745 0.0 38 0.0 0.0 0.0 1.3872326894029732 0.0 39 0.0 0.0 0.0 1.8840925351776772 0.0 40 0.0 0.0 0.0 2.616795187746774 0.0 41 0.0 0.0 0.0 3.5124678697299734 0.0 42 0.0 0.0 0.0 4.520762116755439 0.0 43 0.0 0.0 0.0 5.62577841375838 0.0 44 0.0 0.0 0.0 6.701645599809206 0.0 45 0.0 0.0 0.0 7.6556165036966375 0.0 46 0.0 0.0 0.0 8.601637650051673 0.0 47 0.0 0.0 0.0 9.617881654609535 0.0 48 0.0 0.0 0.0 10.449956276333571 0.0 49 0.0 0.0 0.0 11.317804806953388 0.0 50 0.0 0.0 0.0 12.152529347854891 0.0 51 0.0 0.0 0.0 13.087950817500067 0.0 52 0.0 0.0 0.0 14.057821236452288 0.0 53 0.0 0.0 0.0 15.068765402655218 0.0 54 0.0 0.0 0.0 16.06910989214829 0.0 55 0.0 0.0 0.0 16.94358322071177 0.0 56 0.0 0.0 0.0 17.811431751331586 0.0 57 0.0 0.0 0.0 18.59050798950632 0.0 58 0.0 0.0 0.0 19.271537218114847 0.0 59 0.0 0.0 0.0 19.89161830564168 0.0 60 0.0 0.0 0.0 20.536873625354428 0.0 61 0.0 0.0 0.0 21.29872538887564 0.0 62 0.0 0.0 0.0 21.905556880515146 0.0 63 0.0 0.0 0.0 22.680658239923684 0.0 64 0.0 0.0 0.0 23.53393221506744 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTGAA 30 8.304767E-6 58.333332 25 TGAAGGT 25 2.376697E-4 55.999996 28 TGGCGTG 25 2.376697E-4 55.999996 23 AGTACTA 25 2.376697E-4 55.999996 5 GACAGGG 20 0.006927015 52.5 2 ACAGTTC 20 0.006927015 52.5 8 TCGTTCA 20 0.006927015 52.5 20 TACCCGC 20 0.006927015 52.5 11 ATAGGCG 20 0.006927015 52.5 3 GCAACTG 20 0.006927015 52.5 29 GGCAACT 20 0.006927015 52.5 28 CACGCTA 20 0.006927015 52.5 23 CGTTCAT 20 0.006927015 52.5 21 GACCATG 20 0.006927015 52.5 7 CGGGAGT 20 0.006927015 52.5 1 GGAGTAC 20 0.006927015 52.5 3 CTACCCG 20 0.006927015 52.5 10 GGGCAAA 20 0.006927015 52.5 9 CCACGCT 20 0.006927015 52.5 22 ATGATTC 30 5.844353E-4 46.666664 40 >>END_MODULE