FastQCFastQC Report
Wed 25 May 2016
SRR1780603_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780603_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873509
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTG301.3059023E-436.64246430
CCGAGTT250.002337601535.2454113
CGAGAAT1450.030.38746314
CGTGCGA1500.029.34422910
GTACGCT300.005777249629.29886444
CCGCGTA300.005783718529.2921564
GCGAAGT703.619425E-828.26056932
TCCGGTT406.9707463E-427.54021817
TGTCCCG508.379619E-526.38106527
TTCGGTA757.0565875E-826.38106226
CTCGCTA858.116331E-925.91574313
CATCGTT959.713403E-1025.45103832
CCGTGCG2000.025.2659349
TCGTTCG801.3085992E-724.75067723
CGTTCGG901.5053956E-824.45352624
CCAACGT1001.7425918E-924.20066322
CCCGTGC1950.023.6604068
CCCAACG952.6289854E-823.2024221
CGAACGA952.6435373E-823.19043414
CGCAAAG952.654815E-823.18111812
Produced by FastQC (version 0.11.5)