FastQCFastQC Report
Wed 25 May 2016
SRR1780603_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780603_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873509
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT110011.2594031658517544No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT46200.5289012477261253TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC17470.19999793934578808No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC11710.13405700456434907No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG9480.10852778849445169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAT603.6379788E-1240.33464416
GATGATC250.0023500835.2071911
CGTGCGA1250.033.4468310
CCGTCTT5050.031.36376844
CCGTTTA801.2914825E-1030.25618213
GCCGTCT5300.029.88434243
CGAGAAT1400.029.86324114
ACTGCCG300.005738419529.33932710
TACGTAT300.005738419529.33932713
CGTGGAT300.005740025529.3376483
CTGCGGT300.005740025529.3376483
AGTGGTC300.00574645329.3309298
TACGCTG300.00574645329.33092939
TACGCTC300.00574645329.33092917
CGTCATG300.00574645329.33092923
GATGTAT1450.028.82522424
GTTGTAC1150.028.69338
TTGTACG1100.028.0025149
ATTGGCG2450.027.83445521
CGCCATT2550.027.60558117
Produced by FastQC (version 0.11.5)