FastQCFastQC Report
Wed 25 May 2016
SRR1780602_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780602_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101804
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTTC150.002219809269.99416
GTACAAC200.006936250352.495586
TTGTACA200.006936250352.495584
CGGTGTA200.006936250352.4955834
CCTGGAA200.006936250352.4955844
CGGTACC200.006936250352.4955845
TGTACAA200.006936250352.495585
CAGAGTT200.006936250352.4955834
TGTCGAA200.006936250352.4955812
CAGACAG200.006936250352.4955845
CAAGTCG305.856479E-446.66273518
TGGACGA305.856479E-446.66273555
GCACTTG305.856479E-446.66273550
AGTCGTA305.856479E-446.66273520
AAGTCGT305.856479E-446.66273519
CATGGTA350.001250903639.9966328
TTGGACG350.001250903639.9966354
TACTGGA350.001250903639.9966339
GGAGTTT350.001250903639.9966359
ATAAGCC350.001250903639.996633
Produced by FastQC (version 0.11.5)