##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780602_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 101804 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.86955325920396 34.0 31.0 34.0 12.0 34.0 2 31.0819024792739 34.0 32.0 34.0 23.0 34.0 3 31.25276020588582 34.0 33.0 34.0 23.0 34.0 4 31.586568307728577 34.0 34.0 34.0 23.0 34.0 5 31.55403520490354 34.0 34.0 34.0 23.0 34.0 6 34.51151231778712 38.0 36.0 38.0 25.0 38.0 7 33.307718753683545 37.0 34.0 38.0 21.0 38.0 8 32.442281246316455 37.0 31.0 38.0 11.0 38.0 9 33.34562492632902 37.0 34.0 38.0 21.0 38.0 10-11 33.68117166319594 37.0 34.0 38.0 21.5 38.0 12-13 34.072148442104435 38.0 34.5 38.0 22.5 38.0 14-15 33.72190680130447 37.0 34.0 38.0 21.5 38.0 16-17 33.59515834348356 37.0 34.0 38.0 21.0 38.0 18-19 33.602157086165576 37.0 34.0 38.0 21.5 38.0 20-21 33.66798946996188 37.0 34.0 38.0 21.0 38.0 22-23 33.63840811755924 37.0 34.0 38.0 21.0 38.0 24-25 34.047748614985665 38.0 34.5 38.0 22.5 38.0 26-27 34.137180267965896 38.0 34.5 38.0 23.0 38.0 28-29 34.24944010058544 38.0 35.0 38.0 23.5 38.0 30-31 34.113173352716984 38.0 34.0 38.0 23.0 38.0 32-33 33.863153707123494 38.0 34.0 38.0 22.0 38.0 34-35 33.74217614239126 37.0 34.0 38.0 21.5 38.0 36-37 33.70170130839652 37.0 34.0 38.0 21.5 38.0 38-39 34.23038387489686 38.0 35.0 38.0 23.0 38.0 40-41 33.70988369808652 37.5 34.0 38.0 21.0 38.0 42-43 34.02546068916742 38.0 34.0 38.0 22.5 38.0 44-45 33.138673333071395 37.0 34.0 38.0 21.0 38.0 46-47 33.83723134650897 37.0 34.0 38.0 22.0 38.0 48-49 33.55084770735924 37.0 34.0 38.0 21.0 38.0 50-51 33.53270991316648 37.0 34.0 38.0 21.5 38.0 52-53 33.565572079682525 37.0 34.0 38.0 21.0 38.0 54-55 33.27810793289065 37.0 33.5 38.0 21.0 38.0 56-57 33.31990884444619 37.0 33.5 38.0 21.0 38.0 58-59 33.215752819142665 37.0 33.0 38.0 21.0 38.0 60-61 33.205679541078936 37.0 33.0 38.0 21.0 38.0 62-63 33.313592786138074 37.0 33.0 38.0 21.0 38.0 64-65 33.31235020234961 37.0 33.0 38.0 21.0 38.0 66-67 33.27462084004558 37.0 33.5 38.0 20.5 38.0 68-69 32.89726828022475 37.0 31.5 38.0 15.5 38.0 70-71 32.74654728694354 37.0 31.5 38.0 19.0 38.0 72-73 32.88815763624219 37.0 32.0 38.0 19.0 38.0 74-75 32.52459137165533 37.0 31.0 38.0 15.0 38.0 76 31.839858944638717 36.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.1536273027463757 1101 2 -0.7802324636481508 1101 3 -0.11206201723874187 1101 4 -2.089724180906231 1101 5 -0.7422285686855865 1101 6 -2.593509162650051 1101 7 -1.3207875730865375 1101 8 -0.20106898544525365 1101 9 -0.9796943459671112 1101 10-11 -0.9044133433562962 1101 12-13 -0.8968335825823814 1101 14-15 -0.2112776957904714 1101 16-17 -1.3364337727643658 1101 18-19 -0.14112972927548384 1101 20-21 -1.115177665079635 1101 22-23 -0.4100850165085035 1101 24-25 -1.2582596744326722 1101 26-27 -1.4565149967223334 1101 28-29 -0.5125466338296576 1101 30-31 -1.4103061741422422 1101 32-33 -0.03158095505520464 1101 34-35 -0.6226859888293745 1101 36-37 0.32947880111725425 1101 38-39 -1.1220380657505018 1101 40-41 -0.021897842289725133 1101 42-43 -0.3734817231511016 1101 44-45 0.4708500839300953 1101 46-47 0.6713428764980875 1101 48-49 -0.9023929180105981 1101 50-51 -0.8849370884642767 1101 52-53 -0.2450390534637421 1101 54-55 -1.0899847169252297 1101 56-57 -0.8824393244583248 1101 58-59 -0.4029961869360079 1101 60-61 0.08424791862791636 1101 62-63 -0.942114009291835 1101 64-65 -0.30353299688157875 1101 66-67 -0.7633265142889059 1101 68-69 0.13342395066256785 1101 70-71 0.4409270298378729 1101 72-73 -0.8663100534089203 1101 74-75 -1.5562211861599309 1101 76 -1.7179627310595968 1102 1 0.9890127732270351 1102 2 0.16354721346485945 1102 3 0.3040190210645193 1102 4 0.4483733183023837 1102 5 0.204495078038061 1102 6 0.6737114767928105 1102 7 1.4928243294142547 1102 8 2.118240922753543 1102 9 0.8941264834092735 1102 10-11 0.3594043204613655 1102 12-13 -3.7116167418099835 1102 14-15 -0.5954894716689125 1102 16-17 -1.4143065173037783 1102 18-19 -0.1611123187025214 1102 20-21 0.9004760227962763 1102 22-23 -0.23829014471363763 1102 24-25 0.5303997114489292 1102 26-27 -4.548221232873015 1102 28-29 0.2592482379652168 1102 30-31 0.8162212079406928 1102 32-33 -5.535704095289461 1102 34-35 -0.23987497268502267 1102 36-37 0.5369811752863001 1102 38-39 0.8027720007262218 1102 40-41 0.9256409358046156 1102 42-43 0.4617509454149058 1102 44-45 -5.2390502009701905 1102 46-47 0.18176547858735148 1102 48-49 0.6001870123471136 1102 50-51 -5.793990586406657 1102 52-53 -1.469105213641015 1102 54-55 1.260885811723071 1102 56-57 -0.8064026039771619 1102 58-59 0.9876654148367052 1102 60-61 0.2321032525943636 1102 62-63 -4.7558525341414715 1102 64-65 -0.2549574983060765 1102 66-67 0.5613806715293919 1102 68-69 -6.620904950888551 1102 70-71 -0.07425973812667763 1102 72-73 0.823647211548348 1102 74-75 -5.64809361414347 1102 76 -1.4524198377389261 1103 1 0.08611781871917756 1103 2 -0.417428799769219 1103 3 -0.06493603821312277 1103 4 0.3322856425372862 1103 5 0.17413941219852447 1103 6 -2.046980555189542 1103 7 0.6299624601007707 1103 8 0.5621063762964127 1103 9 -0.10262013914380219 1103 10-11 0.3506643149471671 1103 12-13 -1.7855897222345831 1103 14-15 -1.1549256448147602 1103 16-17 -1.0339646369619047 1103 18-19 -0.09559674109016214 1103 20-21 -0.06805168605401235 1103 22-23 -0.6878627942862892 1103 24-25 0.4686751456921101 1103 26-27 -0.7682102044748902 1103 28-29 0.05196949520260574 1103 30-31 0.07489228596660524 1103 32-33 -5.12563378925141 1103 34-35 -1.348890688152352 1103 36-37 -0.3469449344462703 1103 38-39 0.06533748184008914 1103 40-41 0.26198640037588206 1103 42-43 -0.2024343316736008 1103 44-45 -4.917441433393684 1103 46-47 -0.6299315161741532 1103 48-49 -0.14904099978412688 1103 50-51 -0.5656751742202815 1103 52-53 -0.8779210393923194 1103 54-55 -0.32408799583137693 1103 56-57 0.6646202799489487 1103 58-59 0.30037564367596303 1103 60-61 1.132303142310377 1103 62-63 0.07315215292773303 1103 64-65 0.38762590395474206 1103 66-67 -0.5462151376793187 1103 68-69 -2.8671070462841968 1103 70-71 0.3219837137619308 1103 72-73 0.7017903050140148 1103 74-75 -2.0648498865449056 1103 76 -0.05541129073037965 1104 1 -2.079290554650761 1104 2 -0.1263054870261371 1104 3 0.1639208213918515 1104 4 0.027778664729005698 1104 5 -0.5393128870464992 1104 6 -0.628007017478879 1104 7 0.7023715198125089 1104 8 1.446991604695711 1104 9 -0.28225103977676014 1104 10-11 -0.6158002621900209 1104 12-13 -0.6446227429010989 1104 14-15 -0.19623960805733986 1104 16-17 -0.7532317764418082 1104 18-19 0.4824365083569404 1104 20-21 1.0130510364351153 1104 22-23 -0.3249786335297813 1104 24-25 0.5196932193594606 1104 26-27 1.0261922968596622 1104 28-29 -0.4221211019147617 1104 30-31 -0.8190488411591446 1104 32-33 -0.6242611056549805 1104 34-35 -0.3048504227668545 1104 36-37 -0.19501555139127902 1104 38-39 -0.44440475868457696 1104 40-41 1.0283823433332557 1104 42-43 1.2703979721470375 1104 44-45 -2.6837992462511515 1104 46-47 -0.059219245908010976 1104 48-49 0.5601979234218462 1104 50-51 0.8570732433805759 1104 52-53 -0.12771132384287398 1104 54-55 -0.4364955303391156 1104 56-57 0.5422607893670843 1104 58-59 1.0354286445148233 1104 60-61 -0.005499038731336725 1104 62-63 0.532581726633218 1104 64-65 -0.009403761262980481 1104 66-67 0.5111897277214297 1104 68-69 -3.8665541598356334 1104 70-71 -0.536439225306161 1104 72-73 0.422106022241195 1104 74-75 -0.814582975845596 1104 76 0.10652724354858023 1105 1 -1.5675324359497544 1105 2 -0.272890033498701 1105 3 -0.9173035160474932 1105 4 -0.3966334293359459 1105 5 -0.00967496244776811 1105 6 -0.14416976477264143 1105 7 1.2785193361618923 1105 8 -0.23412075066076454 1105 9 0.15593485048079714 1105 10-11 0.20123967808620336 1105 12-13 -1.4838164719044542 1105 14-15 -0.30510485628428796 1105 16-17 0.44088916420768953 1105 18-19 0.6290023911490294 1105 20-21 -0.3022905089176291 1105 22-23 0.21096761507043738 1105 24-25 -0.274222420804783 1105 26-27 -0.10490139481633776 1105 28-29 -1.098511546110359 1105 30-31 -0.06259120771382953 1105 32-33 0.35252127583065374 1105 34-35 0.4259819773824489 1105 36-37 0.3659960200906127 1105 38-39 -0.011836636250833976 1105 40-41 0.09969626643889029 1105 42-43 0.2736267888696915 1105 44-45 0.8343978422146918 1105 46-47 0.6135131169139356 1105 48-49 0.24397918508665128 1105 50-51 0.2832023960494823 1105 52-53 -0.454699005813751 1105 54-55 -0.9746067658747677 1105 56-57 -0.5266455189569186 1105 58-59 -0.2976247336113431 1105 60-61 0.2403016331611596 1105 62-63 0.180584712822089 1105 64-65 -0.19456613528313227 1105 66-67 0.19721063104356773 1105 68-69 1.1936362097052395 1105 70-71 1.2407537571499816 1105 72-73 0.2640993033162289 1105 74-75 -1.4643216843189109 1105 76 -0.7033645071046486 1106 1 -0.437334894852917 1106 2 0.16469803115111148 1106 3 0.19826493263326483 1106 4 0.18632001727270264 1106 5 0.17130307319251514 1106 6 0.05962910247421149 1106 7 1.0768340205105602 1106 8 0.9027351686651102 1106 9 1.3913402932215106 1106 10-11 0.6296344839986219 1106 12-13 0.1444401933808095 1106 14-15 -0.06534107675673795 1106 16-17 0.536026614131714 1106 18-19 0.09714025549493499 1106 20-21 0.27673283684836036 1106 22-23 0.6693113089508103 1106 24-25 0.7610932305282745 1106 26-27 0.6910579918392799 1106 28-29 -0.320945583960885 1106 30-31 -0.5041300943003719 1106 32-33 0.7684903323301739 1106 34-35 0.7593981950920821 1106 36-37 0.574837020228756 1106 38-39 0.7926114919436671 1106 40-41 0.35963548456293637 1106 42-43 0.6401276867837709 1106 44-45 1.0987299322823816 1106 46-47 0.8332948547230217 1106 48-49 0.9111500650424418 1106 50-51 0.7520724057193249 1106 52-53 -0.15085869637874794 1106 54-55 0.5341444428557196 1106 56-57 1.3045604487181706 1106 58-59 -0.3054296790457869 1106 60-61 0.8672183343629882 1106 62-63 0.9681934985659737 1106 64-65 0.8161812055097855 1106 66-67 1.0762201627468357 1106 68-69 1.2380138861798926 1106 70-71 0.3736657615704715 1106 72-73 0.5420451521903829 1106 74-75 -0.67033770863166 1106 76 0.16287392361782693 1107 1 0.08068604840546456 1107 2 0.15778459120739186 1107 3 0.4407359124205854 1107 4 0.3100042990055272 1107 5 0.2676988845408381 1107 6 0.6973567318813636 1107 7 -0.061867713937580504 1107 8 0.6176065056242805 1107 9 0.07393087146108712 1107 10-11 0.09234114308890895 1107 12-13 0.47561561346361003 1107 14-15 -0.3545893923667691 1107 16-17 0.19341222443557626 1107 18-19 0.8598036210278508 1107 20-21 0.5985595543849556 1107 22-23 -0.07331525705833997 1107 24-25 -0.40572902236021235 1107 26-27 0.18053901032021713 1107 28-29 -0.8596256716303472 1107 30-31 0.34683183442761134 1107 32-33 1.1786562007718686 1107 34-35 0.9214784505240701 1107 36-37 0.14403906584934134 1107 38-39 0.5481923020743622 1107 40-41 0.5575005710147636 1107 42-43 0.27473006251773313 1107 44-45 0.32499288652650904 1107 46-47 0.6063689678815649 1107 48-49 0.9558438984163686 1107 50-51 1.4957001352428207 1107 52-53 1.1933386140089937 1107 54-55 1.1267858910252144 1107 56-57 1.147364247727836 1107 58-59 1.0998250764809043 1107 60-61 0.5964238975851046 1107 62-63 0.23375888536685352 1107 64-65 0.6527480264097534 1107 66-67 1.1402202168637885 1107 68-69 1.4305687472346307 1107 70-71 0.6920831114635959 1107 72-73 0.9320862810698856 1107 74-75 0.6354974511486233 1107 76 -0.7335327111198424 1108 1 -0.9115446403738581 1108 2 -0.16415624249946603 1108 3 -0.14297094549066713 1108 4 -0.5305007062238154 1108 5 -0.12588396321054418 1108 6 0.18073488816404648 1108 7 0.9980082759025493 1108 8 1.266680164671044 1108 9 0.18236505643045575 1108 10-11 0.3363273973844443 1108 12-13 0.5776251756928019 1108 14-15 0.736924129222956 1108 16-17 -0.6305829944498242 1108 18-19 -0.11010897422091404 1108 20-21 0.2711839380694059 1108 22-23 -0.2923313933200973 1108 24-25 -0.7265679585622209 1108 26-27 -0.9893026152587439 1108 28-29 0.4566562647210688 1108 30-31 0.5045712636820525 1108 32-33 0.47078977316093074 1108 34-35 -0.022511539017244786 1108 36-37 0.16811272489610474 1108 38-39 0.9249291913616773 1108 40-41 0.29414706735422413 1108 42-43 0.5662659955821283 1108 44-45 0.028619810178078353 1108 46-47 1.0804276859452102 1108 48-49 0.42883807143295627 1108 50-51 0.0694152106947925 1108 52-53 0.26135743964772473 1108 54-55 0.13134289087580697 1108 56-57 0.4345672956883888 1108 58-59 -1.0484828571811349 1108 60-61 1.041216964968946 1108 62-63 0.23960454501126094 1108 64-65 0.5813301271119826 1108 66-67 0.11071177520832265 1108 68-69 1.3171106566922717 1108 70-71 1.729865122519918 1108 72-73 0.25385902871668264 1108 74-75 1.0381382699144979 1108 76 0.45304281554981074 1109 1 0.2147780114490203 1109 2 -0.2456090282173342 1109 3 0.4349213367419189 1109 4 0.39605764090019946 1109 5 0.7474548863957935 1109 6 0.867097156856687 1109 7 -0.8530899639048855 1109 8 0.890106773166103 1109 9 1.5264927330765587 1109 10-11 1.1667772243740657 1109 12-13 0.2646142935906042 1109 14-15 0.3883207492520384 1109 16-17 0.2303567778785478 1109 18-19 0.8144998718750713 1109 20-21 1.2591797757906846 1109 22-23 1.0685770365480067 1109 24-25 1.0527247047946844 1109 26-27 0.807009004018127 1109 28-29 0.3190565956974538 1109 30-31 0.9348530950846907 1109 32-33 1.1873661735420598 1109 34-35 0.27532332382815383 1109 36-37 0.22968812231850677 1109 38-39 0.8664065495683886 1109 40-41 0.9508405631668708 1109 42-43 1.0044712009379282 1109 44-45 0.4145896712682955 1109 46-47 -0.04295504310549347 1109 48-49 0.03498871583393992 1109 50-51 1.2257325969843436 1109 52-53 1.0910425106070534 1109 54-55 0.7537790776267173 1109 56-57 1.1403368157726348 1109 58-59 0.7240774457124104 1109 60-61 1.0254955869036522 1109 62-63 0.15745595667566903 1109 64-65 0.7767566353111661 1109 66-67 1.1249287151812482 1109 68-69 1.8198123767664924 1109 70-71 0.37647888311366984 1109 72-73 -0.10467991115594089 1109 74-75 1.632096697275216 1109 76 -0.6428776498230384 1110 1 0.6449568291710897 1110 2 0.2628479127655581 1110 3 0.08863440567990111 1110 4 0.438233458162518 1110 5 0.43946011300309706 1110 6 0.2223128753942092 1110 7 0.9305865671764906 1110 8 2.6259332304458525 1110 9 -1.0904889012061076 1110 10-11 -0.7126236515666093 1110 12-13 0.126145495952251 1110 14-15 0.15678325560381978 1110 16-17 0.29198718898992126 1110 18-19 0.8275240449338455 1110 20-21 -0.05162187930162787 1110 22-23 1.0473741909507055 1110 24-25 0.6116934177426359 1110 26-27 -0.6171023517541343 1110 28-29 0.8347727134896914 1110 30-31 0.5351023268218071 1110 32-33 1.364121079535721 1110 34-35 1.3468383140282612 1110 36-37 0.41984830815342633 1110 38-39 0.5435062664604899 1110 40-41 0.5747668099304875 1110 42-43 0.7030096866736386 1110 44-45 1.8153204284004332 1110 46-47 1.1102619820838555 1110 48-49 1.6977394598995588 1110 50-51 0.6825828401667664 1110 52-53 0.28631436677856925 1110 54-55 -0.1328204819832166 1110 56-57 0.5054855079109544 1110 58-59 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2.889409907694379 2116 46-47 1.0833168082985338 2116 48-49 1.5008304209830854 2116 50-51 0.7885372946342315 2116 52-53 1.950961986816523 2116 54-55 0.8153038843567657 2116 56-57 0.727883927335391 2116 58-59 -0.9819656975453341 2116 60-61 -0.9685616029961466 2116 62-63 1.2130636797561536 2116 64-65 1.443541214876653 2116 66-67 0.6490987155671419 2116 68-69 0.5398828672412677 2116 70-71 -2.0321021745007144 2116 72-73 0.8990761506389902 2116 74-75 -1.036662387377671 2116 76 0.07759160063682202 2117 1 0.1266196108338704 2117 2 0.7629774128950579 2117 3 1.1476087646542616 2117 4 0.316037135966198 2117 5 -0.06074708720410271 2117 6 -0.9782828252014895 2117 7 -1.1154377788478556 2117 8 -2.1031265986139793 2117 9 0.2861492754320665 2117 10-11 -0.0710800100229605 2117 12-13 0.6597507795575339 2117 14-15 0.03933958816015348 2117 16-17 0.14936869637143246 2117 18-19 0.7043435217533158 2117 20-21 0.3815301538504059 2117 22-23 1.051026094602605 2117 24-25 1.1810407370899583 2117 26-27 1.2019212172694367 2117 28-29 0.7893052180222 2117 30-31 0.5259078176273064 2117 32-33 1.1737688391834808 2117 34-35 0.10570907289901754 2117 36-37 -0.4637310754259545 2117 38-39 0.3310483540025757 2117 40-41 -0.9134616282979522 2117 42-43 -1.2654570317930762 2117 44-45 0.4838130468930544 2117 46-47 -0.2200151481932764 2117 48-49 -0.332434070273969 2117 50-51 0.8023057098896302 2117 52-53 1.0140144444786472 2117 54-55 -0.6459517951145273 2117 56-57 -0.7575422051167635 2117 58-59 -0.46760524043395435 2117 60-61 1.0770462725373804 2117 62-63 -0.5923197706087038 2117 64-65 -0.08501447836305687 2117 66-67 -1.503861493712769 2117 68-69 -0.12254312752673258 2117 70-71 1.4654126265456924 2117 72-73 0.7744306873318152 2117 74-75 0.1343137932639351 2117 76 -0.01467054998963846 2118 1 0.22251333064063417 2118 2 0.6691770908338697 2118 3 0.2736957211760007 2118 4 0.6434123372544427 2118 5 0.6241160367894523 2118 6 0.7205899606278123 2118 7 0.5813416092358779 2118 8 -4.044914038227507 2118 9 -0.5458958131347629 2118 10-11 -0.1328755011662821 2118 12-13 0.5287121322145438 2118 14-15 -0.6308294939654502 2118 16-17 0.003474976564675103 2118 18-19 -0.6735147713223668 2118 20-21 -3.123743598162477 2118 22-23 -0.47711400201575316 2118 24-25 0.6321276936117002 2118 26-27 0.978088689089077 2118 28-29 -0.24986547440936846 2118 30-31 -0.3106461276222987 2118 32-33 0.11209412098702387 2118 34-35 0.9430681711276847 2118 36-37 -0.10724959394447353 2118 38-39 -0.18195486660933824 2118 40-41 -1.468534092066072 2118 42-43 -0.7851832797801848 2118 44-45 0.13188067974329698 2118 46-47 -0.25109405318522704 2118 48-49 -0.9479735227699493 2118 50-51 0.13100941359333973 2118 52-53 -1.622700531366764 2118 54-55 0.2346037604410256 2118 56-57 1.3421759913405609 2118 58-59 0.7703577225290132 2118 60-61 -1.6290728901035223 2118 62-63 -0.035757773829324435 2118 64-65 -0.6904090033227988 2118 66-67 -2.190012862472834 2118 68-69 0.7754439900256003 2118 70-71 -0.8860769064655827 2118 72-73 -1.2026627104137546 2118 74-75 1.1486455162913103 2118 76 -0.3259426916965644 2119 1 0.0764904118674643 2119 2 0.11095329575466195 2119 3 0.03675605147596528 2119 4 -0.054936162914078324 2119 5 -0.14231470132985535 2119 6 1.1969971058923932 2119 7 -1.602345616918484 2119 8 0.4428682334273617 2119 9 -0.2049790622078973 2119 10-11 0.01849794002010441 2119 12-13 -0.2129882385303219 2119 14-15 -0.7264226857416602 2119 16-17 -0.3776338876078995 2119 18-19 -0.9329519132165345 2119 20-21 -1.3474965472693228 2119 22-23 0.5569692470573813 2119 24-25 -0.4759360070960881 2119 26-27 -0.06918128057724715 2119 28-29 0.053344839038565794 2119 30-31 0.2033410648279883 2119 32-33 -0.05667905056674272 2119 34-35 0.3122120875415675 2119 36-37 -0.5190712993708289 2119 38-39 -0.6779525073238659 2119 40-41 -1.0902058143444648 2119 42-43 -1.163174516719863 2119 44-45 -1.3853084001353722 2119 46-47 -0.610885087900428 2119 48-49 -0.7964736912903376 2119 50-51 0.46143577018248294 2119 52-53 -1.4376651420846684 2119 54-55 -1.3768303480861093 2119 56-57 0.1686421187419782 2119 58-59 0.9438073349321172 2119 60-61 0.18083610888535162 2119 62-63 -0.1842663683692578 2119 64-65 0.35365907891515036 2119 66-67 -0.01643685977651188 2119 68-69 -0.6678488984138937 2119 70-71 -0.5054314733681835 2119 72-73 -1.5071369267939332 2119 74-75 -0.13278353662408904 2119 76 0.21719852839106935 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 17.0 13 87.0 14 305.0 15 681.0 16 937.0 17 1139.0 18 1172.0 19 1149.0 20 982.0 21 968.0 22 877.0 23 922.0 24 945.0 25 1204.0 26 1360.0 27 1600.0 28 2005.0 29 2348.0 30 2842.0 31 3554.0 32 4505.0 33 5986.0 34 8178.0 35 12146.0 36 20466.0 37 25426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.26495472762357 25.645440601652563 11.2552570382465 27.834347632477364 2 17.038623236807986 22.740756748261365 36.83450552041177 23.38611449451888 3 16.635888570193703 25.964598640524933 31.462417979647167 25.937094809634196 4 12.5142430552827 18.998271187772584 38.61930768928529 29.86817806765942 5 11.9887234293348 36.59090016109386 37.35118462928765 14.069191780283683 6 25.992102471415663 41.19779183529134 17.969824368394168 14.840281324898823 7 24.41554359357196 30.31118620093513 23.44112215630034 21.832148049192565 8 21.895996228046048 35.49271148481395 20.665199795685826 21.946092491454166 9 23.191623118934423 16.075006875957722 22.365525912537816 38.36784409257004 10-11 24.256905426112922 25.6949628698283 27.578484146006048 22.46964755805273 12-13 23.453400652233704 23.665081922124866 26.20427488114416 26.677242544497272 14-15 21.01145338100664 25.20480531216848 24.283426977328983 29.500314329495893 16-17 22.02860398412636 27.67327413461161 25.790735138108523 24.50738674315351 18-19 21.691682055714903 26.800518643668227 26.31527248438175 25.192526816235116 20-21 21.147499115948293 27.49990177203253 26.282857255117676 25.069741856901494 22-23 22.823268240933558 26.726847668068054 26.55003732662764 23.899846764370754 24-25 21.682841538642883 27.84566421751601 26.533829711995597 23.937664531845506 26-27 20.882774743625003 28.196829201210168 27.055911359082156 23.86448469608267 28-29 21.797277120741818 27.017111311932734 28.4934776629602 22.69213390436525 30-31 21.949039330478172 26.856017445287023 27.554909433813997 23.64003379042081 32-33 20.146064987623276 28.37216612313858 27.426721936269693 24.055046952968446 34-35 22.220639660524142 27.490570115123177 27.13302031354367 23.155769910809003 36-37 22.375348709284506 27.030372087540766 26.70229067620133 23.8919885269734 38-39 21.619484499626733 27.35255982083219 27.426721936269693 23.601233743271386 40-41 21.688735216690898 26.899237750972453 27.598620879336767 23.81340615299988 42-43 21.12097756473223 26.527936033947586 27.94045420612157 24.410632195198616 44-45 20.213842285175435 26.856017445287023 28.37707752151192 24.553062748025617 46-47 21.168618128953675 26.168912812856078 28.599072727987114 24.063396330203133 48-49 20.572865506266943 26.78922242740953 28.608404384896467 24.029507681427056 50-51 20.330733566461042 26.65415897214255 28.420788967034692 24.594318494361715 52-53 20.039487642921692 26.64089819653452 28.604966406035125 24.714647754508665 54-55 19.386271659266825 27.060331617618168 28.280814113394364 25.27258260972064 56-57 19.707968252720914 26.511237279478213 28.45565989548544 25.325134572315427 58-59 19.90589760716671 26.771050253428157 28.04555813131115 25.27749400809399 60-61 19.763958194177043 26.54414364857962 28.018545440257753 25.67335271698558 62-63 19.841558288475895 26.064791167341166 28.16785195080743 25.925798593375504 64-65 20.245766374602177 26.795116105457545 27.24549133629327 25.71362618364701 66-67 20.460394483517348 26.833425012769634 27.149227928175712 25.556952575537306 68-69 20.161290322580644 26.90856940788181 26.724883108718718 26.205257160818828 70-71 20.654885859101803 27.52494990373659 25.92825429256218 25.89190994459943 72-73 20.5109818867628 27.22142548426388 25.69545400966563 26.57213861930769 74-75 20.134277631527247 28.010687202860396 25.93807708930887 25.916958076303487 76 21.02373187694 27.5863423834034 25.327099131664767 26.06282660799183 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 2.0 14 2.0 15 3.5 16 4.5 17 3.0 18 10.0 19 13.0 20 10.5 21 12.0 22 31.0 23 66.0 24 92.5 25 103.0 26 164.0 27 295.0 28 433.5 29 502.0 30 575.5 31 804.5 32 1183.0 33 1406.0 34 1645.0 35 2232.0 36 2816.0 37 3052.0 38 3488.5 39 4524.5 40 5555.5 41 6145.0 42 6303.0 43 6763.5 44 7359.5 45 7692.5 46 7890.0 47 7945.5 48 7611.0 49 6894.0 50 6567.0 51 6090.0 52 4991.0 53 4011.5 54 3654.0 55 3381.0 56 2660.0 57 1897.0 58 1582.0 59 1309.0 60 894.5 61 633.5 62 514.0 63 438.5 64 332.0 65 239.5 66 151.0 67 124.0 68 104.5 69 71.0 70 45.0 71 33.0 72 35.5 73 30.5 74 20.0 75 17.0 76 14.5 77 13.0 78 10.0 79 6.0 80 6.0 81 9.0 82 7.5 83 3.0 84 3.0 85 3.5 86 4.0 87 4.0 88 3.0 89 3.5 90 4.5 91 4.0 92 4.0 93 3.0 94 1.5 95 1.0 96 1.0 97 2.5 98 4.0 99 5.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7357274763270598 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 101804.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.69058190247927 #Duplication Level Percentage of deduplicated Percentage of total 1 75.09043825488762 37.312875721975566 2 8.480439638642338 8.427959608659778 3 4.8372111412022845 7.210915091744921 4 2.903908118686619 5.771875368354878 5 2.0123747207780656 4.999803544065067 6 1.39363868187479 4.155043023849751 7 1.101073398303912 3.829908451534321 8 0.7452507561231146 2.9625554988016187 9 0.555478680293356 2.4841852972378295 >10 2.8623954770988593 22.298730894660327 >50 0.017791132109039872 0.5461474991159483 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.017681034144041492 0.0 2 0.0 0.0 0.0 0.020627873168048406 0.0 3 0.0 0.0 0.0 0.02259243251738635 0.0 4 0.0 0.0 0.0 0.029468390240069153 0.0 5 0.0 0.0 0.0 0.03241522926407607 0.0 6 0.0 0.0 0.0 0.03339750893874504 0.0 7 0.0 0.0 0.0 0.03339750893874504 0.0 8 0.0 0.0 0.0 0.035362068288082985 0.0 9 0.0 0.0 0.0 0.07465325527484186 0.0 10 0.0 0.0 0.0 0.09626340811755923 0.0 11 0.0 0.0 0.0 0.10510392518957998 0.0 12 0.0 0.0 0.0 0.11689128128560763 0.0 13 0.0 0.0 0.0 0.12573179835762838 0.0 14 0.0 0.0 0.0 0.1316254764056422 0.0 15 0.0 0.0 0.0 0.13948371380299399 0.0 16 0.0 0.0 0.0 0.1463596715256768 0.0 17 0.0 0.0 0.0 0.16600526501905621 0.0 18 0.0 0.0 0.0 0.16993438371773212 0.0 19 0.0 0.0 0.0 0.18663313818710464 0.0 20 0.0 0.0 0.0 0.19350909590978743 0.0 21 0.0 0.0 0.0 0.20431417233114613 0.0 22 0.0 0.0 0.0 0.2121724097284979 0.0 23 0.0 0.0 0.0 0.22788888452320144 0.0 24 0.0 0.0 0.0 0.23083572354720835 0.0 25 0.0 0.0 0.0 0.23574712192055322 0.0 26 0.0 0.0 0.0 0.25539271541393266 0.0 27 0.0 0.0 0.0 0.2868256650033397 0.0 28 0.0 0.0 0.0 0.3172763349180779 0.0 29 0.0 0.0 0.0 0.3447801658088091 0.0 30 0.0 0.0 0.0 0.3683548780008644 0.0 31 0.0 0.0 0.0 0.3968409885662646 0.0 32 0.0 0.0 0.0 0.43711445522769243 0.0 33 0.0 0.0 0.0 0.485246159286472 0.0 34 0.0 0.0 0.0 0.5333778633452516 0.0 35 0.0 0.0 0.0 0.6325881104868178 0.0 36 0.0 0.0 0.0 0.7524262307964323 0.0 37 0.0 0.0 0.0 1.0176417429570548 0.0 38 0.0 0.0 0.0 1.3722447055125535 0.0 39 0.0 0.0 0.0 1.8221287965109425 0.0 40 0.0 0.0 0.0 2.29264076067738 0.0 41 0.0 0.0 0.0 2.8368237004439902 0.0 42 0.0 0.0 0.0 3.4291383442693806 0.0 43 0.0 0.0 0.0 4.03127578484146 0.0 44 0.0 0.0 0.0 4.5764410042827395 0.0 45 0.0 0.0 0.0 5.290558327767082 0.0 46 0.0 0.0 0.0 5.944756591096617 0.0 47 0.0 0.0 0.0 6.606813091823504 0.0 48 0.0 0.0 0.0 7.235472083611646 0.0 49 0.0 0.0 0.0 7.82385760873836 0.0 50 0.0 0.0 0.0 8.440729244430475 0.0 51 0.0 0.0 0.0 9.147970610192134 0.0 52 0.0 0.0 0.0 9.821814467015049 0.0 53 0.0 0.0 0.0 10.390554398648383 0.0 54 0.0 0.0 0.0 10.970099406703076 0.0 55 0.0 0.0 0.0 11.49267219362697 0.0 56 0.0 0.0 0.0 12.128207143137795 0.0 57 0.0 0.0 0.0 12.648815370712349 0.0 58 0.0 0.0 0.0 13.23032493811638 0.0 59 0.0 0.0 0.0 13.74700404699226 0.0 60 0.0 0.0 0.0 14.297080664806884 0.0 61 0.0 0.0 0.0 14.897253546029626 0.0 62 0.0 0.0 0.0 15.419826332953518 0.0 63 0.0 0.0 0.0 15.932576323130721 0.0 64 0.0 0.0 0.0 16.444344033633257 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCTTC 15 0.0022198092 69.9941 6 GTACAAC 20 0.0069362503 52.49558 6 TTGTACA 20 0.0069362503 52.49558 4 CGGTGTA 20 0.0069362503 52.49558 34 CCTGGAA 20 0.0069362503 52.49558 44 CGGTACC 20 0.0069362503 52.49558 45 TGTACAA 20 0.0069362503 52.49558 5 CAGAGTT 20 0.0069362503 52.49558 34 TGTCGAA 20 0.0069362503 52.49558 12 CAGACAG 20 0.0069362503 52.49558 45 CAAGTCG 30 5.856479E-4 46.662735 18 TGGACGA 30 5.856479E-4 46.662735 55 GCACTTG 30 5.856479E-4 46.662735 50 AGTCGTA 30 5.856479E-4 46.662735 20 AAGTCGT 30 5.856479E-4 46.662735 19 CATGGTA 35 0.0012509036 39.99663 28 TTGGACG 35 0.0012509036 39.99663 54 TACTGGA 35 0.0012509036 39.99663 39 GGAGTTT 35 0.0012509036 39.99663 59 ATAAGCC 35 0.0012509036 39.99663 3 >>END_MODULE