FastQCFastQC Report
Wed 25 May 2016
SRR1780602_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780602_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101804
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT15051.4783309103768025No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT6460.6345526698361558Illumina PCR Primer Index 10 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC2840.27896742760598797No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2040.20038505363247022No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1120.11001532356292483No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAGT150.002219067770.000014
TAAGTGC207.894012E-570.043
TACCTCG252.3806634E-456.00000436
AGTTAGA252.3806634E-456.00000436
AGAGATG252.3806634E-456.00000432
TCCGGCT200.006933941552.534
AGACTCT200.006933941552.527
AACCGAC200.006933941552.534
CTGTATT200.006933941552.559
GTACATG200.006933941552.51
TACCAGC200.006933941552.517
CCGGCTG200.006933941552.535
TACATGC200.006933941552.52
CGCTCTA200.006933941552.535
TCTAAGA200.006933941552.538
CGGGCGC200.006933941552.512
AGCTCCG200.006933941552.521
CCTATGG200.006933941552.52
TTCGCTC200.006933941552.533
TGAGCGG200.006933941552.58
Produced by FastQC (version 0.11.5)