##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780602_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 101804 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.95656359278614 34.0 34.0 34.0 31.0 34.0 2 33.309663667439395 34.0 34.0 34.0 33.0 34.0 3 33.507681427055914 34.0 34.0 34.0 34.0 34.0 4 33.55184472122903 34.0 34.0 34.0 34.0 34.0 5 33.5700168952104 34.0 34.0 34.0 34.0 34.0 6 37.299722997131745 38.0 38.0 38.0 36.0 38.0 7 36.99094338139955 38.0 37.0 38.0 36.0 38.0 8 36.96868492397155 38.0 37.0 38.0 35.0 38.0 9 36.81294448155279 38.0 37.0 38.0 35.0 38.0 10-11 36.541751797571806 38.0 37.0 38.0 34.0 38.0 12-13 36.444589603551925 38.0 37.0 38.0 34.0 38.0 14-15 36.69278220895053 38.0 37.0 38.0 34.5 38.0 16-17 36.79631939805901 38.0 37.0 38.0 34.5 38.0 18-19 36.812325645357745 38.0 37.0 38.0 34.0 38.0 20-21 36.567291069113196 38.0 37.0 38.0 34.0 38.0 22-23 36.64533318926565 38.0 37.0 38.0 34.0 38.0 24-25 36.04466916820557 38.0 36.5 38.0 32.5 38.0 26-27 36.25208734430868 38.0 37.0 38.0 34.0 38.0 28-29 36.14454245412754 38.0 37.0 38.0 34.0 38.0 30-31 35.88654178617736 38.0 36.5 38.0 32.5 38.0 32-33 36.33915661467133 38.0 37.0 38.0 34.0 38.0 34-35 36.3881920160308 38.0 37.0 38.0 34.0 38.0 36-37 36.50209716710542 38.0 37.0 38.0 34.0 38.0 38-39 36.16305842599505 38.0 37.0 38.0 33.0 38.0 40-41 35.759719657380856 38.0 36.5 38.0 31.5 38.0 42-43 36.10776590310793 38.0 37.0 38.0 33.0 38.0 44-45 36.28566657498723 38.0 37.0 38.0 34.0 38.0 46-47 36.5353227771011 38.0 37.0 38.0 34.0 38.0 48-49 36.36289340300971 38.0 37.0 38.0 34.0 38.0 50-51 36.48436210757927 38.0 37.0 38.0 34.0 38.0 52-53 36.417375545165214 38.0 37.0 38.0 34.0 38.0 54-55 36.57730049899807 38.0 37.0 38.0 34.0 38.0 56-57 36.60748104200228 38.0 37.0 38.0 34.0 38.0 58-59 36.661938627165924 38.0 37.0 38.0 34.0 38.0 60-61 36.38687084986837 38.0 37.0 38.0 34.0 38.0 62-63 36.60864013201839 38.0 37.0 38.0 34.0 38.0 64-65 36.64916407999686 38.0 37.0 38.0 34.0 38.0 66-67 36.08250658127382 38.0 37.0 38.0 33.0 38.0 68-69 35.551491100546144 38.0 36.5 38.0 31.0 38.0 70-71 35.57281148088484 38.0 36.0 38.0 31.0 38.0 72-73 35.27065243015991 38.0 36.0 38.0 29.0 38.0 74-75 35.79357883776669 38.0 37.0 38.0 32.0 38.0 76 35.28199284900397 38.0 36.0 38.0 30.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2975346772816394 1101 2 0.39981654357225693 1101 3 0.16279473924908672 1101 4 0.14922785772699854 1101 5 0.09571579846993927 1101 6 -1.237617777784635 1101 7 0.12031969257181885 1101 8 0.2752505613148202 1101 9 0.10190400438724367 1101 10-11 -0.030229386088691967 1101 12-13 0.5076295933831219 1101 14-15 0.4978003504990838 1101 16-17 0.04312653747398798 1101 18-19 -0.5072407615676369 1101 20-21 -0.3336820758464327 1101 22-23 0.3331490064995606 1101 24-25 -1.6089932423705164 1101 26-27 -0.4661614956050357 1101 28-29 0.6128373254105455 1101 30-31 0.9322557312407866 1101 32-33 0.05558983214541513 1101 34-35 0.04696089224727018 1101 36-37 0.32495919142721164 1101 38-39 0.42294360231627337 1101 40-41 0.8142621702295259 1101 42-43 0.7935258358990396 1101 44-45 0.19887916622489854 1101 46-47 0.5861702182637814 1101 48-49 -1.2955555841453616 1101 50-51 -0.6491512885075466 1101 52-53 -1.3067052257106013 1101 54-55 -0.9732323001564822 1101 56-57 -0.7318970538745546 1101 58-59 -1.2439219626588311 1101 60-61 -1.5021212147118277 1101 62-63 -0.7863507646353582 1101 64-65 -0.9895084006801156 1101 66-67 -0.8436761392352352 1101 68-69 -0.49577100855502465 1101 70-71 -0.9023622741919937 1101 72-73 -1.043683758759606 1101 74-75 -0.9387418012089057 1101 76 -0.8872239214186166 1102 1 -0.8474953955262094 1102 2 0.08457437833009607 1102 3 0.11535884181319034 1102 4 0.3447169365494105 1102 5 0.10516499125246526 1102 6 0.4517301171188137 1102 7 -0.05948404278747432 1102 8 0.3921071615047538 1102 9 0.44859599552367513 1102 10-11 0.4935993571845003 1102 12-13 0.41707087226884454 1102 14-15 -0.1057292474749616 1102 16-17 0.35027278628690084 1102 18-19 0.0827323311832373 1102 20-21 0.7579608491298231 1102 22-23 0.6573022195416556 1102 24-25 -4.050477889410722 1102 26-27 -0.8399585832910077 1102 28-29 0.081078857540966 1102 30-31 1.3323269563120164 1102 32-33 0.5980083190083434 1102 34-35 0.889284412348573 1102 36-37 -2.41095407226382 1102 38-39 -0.1566306300357425 1102 40-41 0.5624973712425003 1102 42-43 0.6713669248512346 1102 44-45 0.8703574821476536 1102 46-47 0.9338831020877834 1102 48-49 -2.807687587944031 1102 50-51 -0.7195058310843123 1102 52-53 -0.07241431086413286 1102 54-55 0.22702094454120214 1102 56-57 0.46786552922135627 1102 58-59 0.28261333332091 1102 60-61 -5.203260815851426 1102 62-63 -2.547857570586615 1102 64-65 0.06945804439744308 1102 66-67 0.8506970801379907 1102 68-69 -0.09080899525967823 1102 70-71 -0.8153014954645528 1102 72-73 -5.6984083551508675 1102 74-75 -1.9934426559097673 1102 76 -0.4307503538339432 1103 1 0.8392843122212383 1103 2 0.45238524130224533 1103 3 0.19505280376521483 1103 4 0.2969777581650703 1103 5 0.10487549580167865 1103 6 0.3120968113564757 1103 7 0.7091953065370404 1103 8 0.548448490426722 1103 9 0.5043996900370473 1103 10-11 0.1697573389876439 1103 12-13 -0.18067519886432848 1103 14-15 -0.13946236024032999 1103 16-17 0.07936707909087914 1103 18-19 0.45041223192765045 1103 20-21 -0.2468640750499418 1103 22-23 0.4678894817418211 1103 24-25 -3.196342240113779 1103 26-27 -0.455143308959606 1103 28-29 -0.1293636569338119 1103 30-31 0.938826818457045 1103 32-33 0.8917772740676213 1103 34-35 -0.0814076196337794 1103 36-37 -1.0448801819963904 1103 38-39 -0.1738969658504672 1103 40-41 -0.2687061027029074 1103 42-43 -0.9421704338474157 1103 44-45 0.7985074683621605 1103 46-47 0.7053126581947566 1103 48-49 -2.3043170337670205 1103 50-51 -0.631761138500913 1103 52-53 -0.001315606700913463 1103 54-55 -0.18483458344336157 1103 56-57 0.2814442444129739 1103 58-59 -0.32290775849050135 1103 60-61 -3.933423484723768 1103 62-63 -1.139861981945863 1103 64-65 0.119478722643926 1103 66-67 -0.5617167605016604 1103 68-69 -0.0690485872089397 1103 70-71 -0.1134335605321013 1103 72-73 -4.919231349360956 1103 74-75 -2.2487776435028124 1103 76 -3.0745330943908726 1104 1 0.41630711402098086 1104 2 0.213843336322455 1104 3 0.20180183144766772 1104 4 -0.11292081576919344 1104 5 -0.07620434862751324 1104 6 0.20647643463109233 1104 7 0.4358787504476638 1104 8 0.4114470414829299 1104 9 0.617226655643698 1104 10-11 -0.9108878223026764 1104 12-13 0.3269317560658962 1104 14-15 0.5049363280842343 1104 16-17 0.1661893165651378 1104 18-19 0.6036952335291161 1104 20-21 -0.1330647918958121 1104 22-23 0.2148581387997055 1104 24-25 0.5006950511239268 1104 26-27 0.7793949355305898 1104 28-29 1.065271438010143 1104 30-31 0.7257939503109228 1104 32-33 0.4603923888392245 1104 34-35 0.3162893223322101 1104 36-37 1.0467655131153322 1104 38-39 1.0586585134448896 1104 40-41 1.4224400881001174 1104 42-43 0.5617379013924264 1104 44-45 0.5215304226823037 1104 46-47 0.613247531984122 1104 48-49 -0.04608093218843834 1104 50-51 0.2267571258169383 1104 52-53 0.13660096464050753 1104 54-55 -0.03986394362028278 1104 56-57 0.1200204664826785 1104 58-59 -0.42048268413681456 1104 60-61 0.2959481312298564 1104 62-63 0.13173871964797002 1104 64-65 0.4467493700930234 1104 66-67 -0.020784098258090467 1104 68-69 1.1727343762624187 1104 70-71 1.0049794647100327 1104 72-73 0.9571699318103981 1104 74-75 0.0423456692402695 1104 76 -0.554591052142726 1105 1 0.970655751572302 1105 2 0.32067424337732575 1105 3 0.32068947609119647 1105 4 0.00861707150217228 1105 5 0.0026683650716279317 1105 6 0.24343052197711756 1105 7 0.7437548236092866 1105 8 -0.2068806927462603 1105 9 0.6153976149568763 1105 10-11 0.4399893706797755 1105 12-13 -0.08141090910767446 1105 14-15 0.3726108334967009 1105 16-17 0.11785038412765658 1105 18-19 0.4923477157986156 1105 20-21 0.2818474888059441 1105 22-23 0.2057502627265464 1105 24-25 0.7216543643004769 1105 26-27 0.6002236029437995 1105 28-29 0.7452826362184268 1105 30-31 1.2084740548275335 1105 32-33 0.829317360843703 1105 34-35 -0.6329827941291626 1105 36-37 0.5277098953475132 1105 38-39 -0.23824331559578837 1105 40-41 0.4955270608511455 1105 42-43 0.6673183418552853 1105 44-45 0.8679283323500897 1105 46-47 0.9950504435182808 1105 48-49 -0.16696559064308758 1105 50-51 0.640885531938622 1105 52-53 0.3452982397431583 1105 54-55 0.7862652090486222 1105 56-57 0.4356792944035419 1105 58-59 0.5085580026866268 1105 60-61 0.8362803447423701 1105 62-63 0.08007765208544981 1105 64-65 0.6320423898765242 1105 66-67 0.7350762974119363 1105 68-69 0.5883745405028122 1105 70-71 0.9384434438067046 1105 72-73 1.0414364491676196 1105 74-75 -0.23581782460072276 1105 76 0.5579136137773943 1106 1 -1.2945880666346312 1106 2 -0.059792671942467734 1106 3 0.14573437179502235 1106 4 0.2245170576881108 1106 5 0.034813689011400584 1106 6 0.19742969313335124 1106 7 -0.7935845879116386 1106 8 0.24774011123218997 1106 9 -0.37805451931581757 1106 10-11 0.325988818117267 1106 12-13 1.0304415567025202 1106 14-15 0.015379171664953617 1106 16-17 0.1796337796242753 1106 18-19 0.440379211861611 1106 20-21 0.1490874390753092 1106 22-23 0.394885502400534 1106 24-25 -0.6361229530085382 1106 26-27 0.6400081035654352 1106 28-29 0.5731594147790062 1106 30-31 0.3973330132472057 1106 32-33 -0.05341506076148761 1106 34-35 0.5232335340929666 1106 36-37 0.841468701812488 1106 38-39 0.18172491205995556 1106 40-41 0.9962799272691569 1106 42-43 0.6870241023194339 1106 44-45 0.8827439146128313 1106 46-47 0.9212695345529553 1106 48-49 0.8491416064835846 1106 50-51 -0.2007293319928536 1106 52-53 0.41691034086209555 1106 54-55 -0.05759444007418324 1106 56-57 0.19094245229828033 1106 58-59 0.491925871176754 1106 60-61 0.8401068401146574 1106 62-63 0.6307614481898867 1106 64-65 -0.6288914407630628 1106 66-67 0.16325007832090677 1106 68-69 1.259599847865701 1106 70-71 0.4071545917553152 1106 72-73 -0.36362640481773667 1106 74-75 -0.7480156419788102 1106 76 0.17797163284080142 1107 1 0.8388778532181718 1107 2 -0.15268441099055252 1107 3 0.32774319285733355 1107 4 0.19606507294908937 1107 5 0.073060841107079 1107 6 0.1707229799261114 1107 7 0.6222500306991776 1107 8 0.2751232862734554 1107 9 -0.28161812025744837 1107 10-11 0.4258819371474871 1107 12-13 -0.051532088344423244 1107 14-15 0.2553201841930317 1107 16-17 0.35577767658561044 1107 18-19 0.5377085405408906 1107 20-21 0.7080005002004057 1107 22-23 0.38397417301773373 1107 24-25 0.8904552324502362 1107 26-27 0.1911080305075643 1107 28-29 0.4893593227801958 1107 30-31 0.6078952883236468 1107 32-33 0.9704640419795396 1107 34-35 0.25399495953334394 1107 36-37 0.8189560519762011 1107 38-39 0.5248060604215752 1107 40-41 0.6801354891383511 1107 42-43 0.7808831817901734 1107 44-45 0.27297445280586885 1107 46-47 0.5116718940518297 1107 48-49 0.6159246245857091 1107 50-51 0.6737667039407924 1107 52-53 0.7033919275709763 1107 54-55 0.43896913380898184 1107 56-57 0.6007362667312677 1107 58-59 0.5067674438152281 1107 60-61 0.7684018857081796 1107 62-63 0.5143153080811089 1107 64-65 0.5976962151613847 1107 66-67 -0.07143482575681048 1107 68-69 0.92143657703744 1107 70-71 0.34385129332741826 1107 72-73 0.914379115110826 1107 74-75 -0.8402309191928694 1107 76 0.2295721415401033 1108 1 -0.17781869541472872 1108 2 0.37364907175260953 1108 3 0.20264981171285967 1108 4 0.35346833008340894 1108 5 0.025399104964840546 1108 6 -0.09810265880091862 1108 7 0.021062222541402775 1108 8 -0.49273118855099085 1108 9 -0.3101553980103233 1108 10-11 0.46592377190021494 1108 12-13 0.8260452312431994 1108 14-15 0.46118825531210206 1108 16-17 0.13196174949760575 1108 18-19 0.4713332230449936 1108 20-21 0.9647612950607041 1108 22-23 0.365914259675435 1108 24-25 1.136757339128856 1108 26-27 -0.06796861673582555 1108 28-29 -0.25757777568087903 1108 30-31 0.6275053286642986 1108 32-33 0.7347140046270795 1108 34-35 0.5484817367632928 1108 36-37 0.919452839664828 1108 38-39 0.9761731603767458 1108 40-41 0.8204126443751676 1108 42-43 0.1244884410162328 1108 44-45 0.6902014063394049 1108 46-47 0.7015531132360522 1108 48-49 0.9408910073736934 1108 50-51 0.6903325189714238 1108 52-53 0.2382044182328542 1108 54-55 0.06378237152001986 1108 56-57 0.49529027838523376 1108 58-59 0.329324593075647 1108 60-61 1.02062435694679 1108 62-63 0.9340822287444865 1108 64-65 0.6836442205401383 1108 66-67 0.6953737802494686 1108 68-69 1.4375354863456806 1108 70-71 -0.270652666033115 1108 72-73 0.37372096480454076 1108 74-75 -0.3378963905808874 1108 76 0.40146815229761046 1109 1 -0.778703806492409 1109 2 0.20476335889969732 1109 3 0.22623187881079332 1109 4 0.20826498020125683 1109 5 0.0637622763203396 1109 6 -0.14447696299829715 1109 7 0.22851169849546693 1109 8 0.5815002943234902 1109 9 0.5530410314566012 1109 10-11 0.4985700785047058 1109 12-13 0.582821471150929 1109 14-15 0.6306694749099151 1109 16-17 0.8513840452701373 1109 18-19 0.7909636327536305 1109 20-21 0.9225868805378639 1109 22-23 0.3738313651365459 1109 24-25 0.20809277867552112 1109 26-27 0.2079453294052911 1109 28-29 -0.4490049859511842 1109 30-31 0.5544523222955178 1109 32-33 0.49154039247121517 1109 34-35 -0.04622264115155872 1109 36-37 1.0369431861870737 1109 38-39 0.9360297372786093 1109 40-41 0.3893472514660843 1109 42-43 0.7781542551679834 1109 44-45 0.6571288422754193 1109 46-47 0.7293914864370024 1109 48-49 0.5846400670998833 1109 50-51 0.05511487692739081 1109 52-53 0.01532191484787404 1109 54-55 -0.12506848851708696 1109 56-57 0.0274476426096939 1109 58-59 -0.012828508574216357 1109 60-61 0.6378890377551798 1109 62-63 0.33870085368807423 1109 64-65 0.7306704479108888 1109 66-67 0.2536582193447998 1109 68-69 -0.38527931732514986 1109 70-71 -0.09679603898331379 1109 72-73 0.12627224106952184 1109 74-75 -0.2788033160109009 1109 76 -0.3626108808501769 1110 1 -0.819173717204535 1110 2 -0.7094815657258522 1110 3 -0.11226353580918413 1110 4 0.08352812536060128 1110 5 0.2873328234203001 1110 6 0.0632685786572793 1110 7 0.3985579152544858 1110 8 0.549100168497759 1110 9 0.6206239675516514 1110 10-11 -0.8240579854728409 1110 12-13 0.4294834596814283 1110 14-15 0.7236413818956677 1110 16-17 0.2966014576155729 1110 18-19 -0.347862074411168 1110 20-21 0.6285902197591966 1110 22-23 -0.7640264517870108 1110 24-25 -0.39803033696316703 1110 26-27 0.8595169411845092 1110 28-29 0.25083410229620995 1110 30-31 -0.03672898774392763 1110 32-33 0.6224838434838773 1110 34-35 0.7071165801807382 1110 36-37 0.9019829906732397 1110 38-39 0.8459917475866376 1110 40-41 -0.930334796589662 1110 42-43 -0.19017153668722386 1110 44-45 0.568609230399403 1110 46-47 0.5616803048849803 1110 48-49 1.0211585659021267 1110 50-51 -0.2527226143010992 1110 52-53 0.43720107375125394 1110 54-55 -0.7394126218923702 1110 56-57 0.24513825649408716 1110 58-59 -0.4990300233224474 1110 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40-41 -0.6411128661037964 2116 42-43 -0.7978352484401583 2116 44-45 -0.18529021587922045 2116 46-47 -0.7294783505859428 2116 48-49 -0.2835311681556405 2116 50-51 -0.7656167833291008 2116 52-53 0.09419278092883587 2116 54-55 -0.27506655903329147 2116 56-57 -0.008871674095772164 2116 58-59 -1.6021378820065166 2116 60-61 -0.9171012505097096 2116 62-63 0.21774151434333078 2116 64-65 -1.4646640602636936 2116 66-67 -0.4786066087381897 2116 68-69 -0.9133211485339103 2116 70-71 0.9764986189192086 2116 72-73 0.4203577300650281 2116 74-75 0.15894510502818804 2116 76 -0.23715584425430336 2117 1 0.5905387925079779 2117 2 0.3738906176463317 2117 3 -0.003871927417577581 2117 4 -1.548583223822675E-4 2117 5 0.26485160093907467 2117 6 0.25044806583676404 2117 7 0.5989205156170883 2117 8 0.32957154896913465 2117 9 -0.8083840614563798 2117 10-11 -0.5090359704508316 2117 12-13 -1.1378436576456892 2117 14-15 0.16364397189826008 2117 16-17 -0.8148981538840374 2117 18-19 -1.0613559879050882 2117 20-21 -0.15713886596989113 2117 22-23 0.24035065259008803 2117 24-25 -0.9766887156215489 2117 26-27 -1.469659437991865 2117 28-29 -0.8632943618322528 2117 30-31 -0.28626278727772103 2117 32-33 -1.5472908262907978 2117 34-35 0.3465493696135269 2117 36-37 -0.8449996563094118 2117 38-39 0.06039216198704622 2117 40-41 0.09903583278097017 2117 42-43 0.24928715277146551 2117 44-45 -0.6040014922113173 2117 46-47 -0.8710314278267433 2117 48-49 -0.24864200389844626 2117 50-51 -0.049562811141292684 2117 52-53 0.6609902475404255 2117 54-55 -0.24092777340752036 2117 56-57 0.37427578563161745 2117 58-59 -0.5523581766505998 2117 60-61 -0.27063973323033963 2117 62-63 -0.12235899508804238 2117 64-65 -0.7189322689928659 2117 66-67 -0.5047872503463395 2117 68-69 0.17336479391411785 2117 70-71 -0.3622388174018738 2117 72-73 0.9217483400058271 2117 74-75 0.6686656061985019 2117 76 0.5518707287871436 2118 1 -0.17781869541472872 2118 2 0.2214751587091328 2118 3 -0.308219753504531 2118 4 0.09622195327181515 2118 5 0.24641359771846538 2118 6 0.010592993372995352 2118 7 0.7312071500776369 2118 8 0.5652398259417595 2118 9 -1.1905901806190187 2118 10-11 -0.2478443440418161 2118 12-13 -0.8025779571625975 2118 14-15 0.46118825531210206 2118 16-17 -2.4658643374589175 2118 18-19 -0.3040290957955847 2118 20-21 -0.08234015421465557 2118 22-23 0.5778707814145676 2118 24-25 0.9809602376795823 2118 26-27 -1.6295628196343799 2118 28-29 0.1753932388118713 2118 30-31 -0.3543787293067169 2118 32-33 0.10427922201837703 2118 34-35 0.03217738893719968 2118 36-37 -2.225474696567062 2118 38-39 0.03052098646370638 2118 40-41 0.20265902118676138 2118 42-43 0.6281116294220297 2118 44-45 -1.6087116371388532 2118 46-47 0.6073502146853329 2118 48-49 -0.01744232595963524 2118 50-51 -0.4908269013184352 2118 52-53 -1.2726651469845365 2118 54-55 -0.3927393676104174 2118 56-57 0.3485511479504524 2118 58-59 -0.6054580156200018 2118 60-61 -0.7130712952271239 2118 62-63 -0.527874292994639 2118 64-65 -0.08990650409753442 2118 66-67 0.2714607367712105 2118 68-69 -1.0135514701760613 2118 70-71 -0.004348318207021862 2118 72-73 1.294010819877002 2118 74-75 0.5606543340567924 2118 76 0.39422177548601667 2119 1 0.5290400844976375 2119 2 -0.594584834550048 2119 3 -0.1434068111385045 2119 4 -0.46604633119060423 2119 5 -0.23833530689899618 2119 6 -0.5386992209849382 2119 7 -0.7848865472597453 2119 8 0.3020952354463162 2119 9 0.12951430202338088 2119 10-11 -0.07367851997710062 2119 12-13 -0.016009032322692462 2119 14-15 -0.3469934096693592 2119 16-17 -0.4880247688711208 2119 18-19 0.01599112665132907 2119 20-21 -1.07104928801985 2119 22-23 -0.198839700553755 2119 24-25 0.5460933687884477 2119 26-27 0.24649043280916771 2119 28-29 -0.019711243830528247 2119 30-31 0.9835046545827382 2119 32-33 0.6322957370166904 2119 34-35 0.4300119019132751 2119 36-37 0.034931437575174584 2119 38-39 -1.8505036175133753 2119 40-41 -0.909491299001985 2119 42-43 0.16552315621677138 2119 44-45 0.11344036825379789 2119 46-47 0.17522180214741212 2119 48-49 -0.30531728382953816 2119 50-51 0.44880066861752965 2119 52-53 0.21478008388840664 2119 54-55 0.1633357924839558 2119 56-57 -0.8129335166939669 2119 58-59 -0.05502828862303488 2119 60-61 0.26704329863873255 2119 62-63 -0.2605588228227447 2119 64-65 -0.23723545590070927 2119 66-67 0.613472870239363 2119 68-69 0.7961468783241088 2119 70-71 -0.09307430405131356 2119 72-73 0.45473714276207744 2119 74-75 -0.41419399759133313 2119 76 -0.184477247095721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 3.0 21 3.0 22 6.0 23 19.0 24 36.0 25 67.0 26 118.0 27 246.0 28 427.0 29 675.0 30 1201.0 31 1883.0 32 3090.0 33 4826.0 34 7654.0 35 12293.0 36 22627.0 37 46629.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.730423166083845 23.32717771403874 11.458292405013555 29.484106714863856 2 16.78421280106872 19.49825154217909 39.82652940945346 23.891006247298733 3 16.56516443361754 25.984244234018313 30.976189540686022 26.474401791678126 4 12.538800047149422 19.781148088483754 39.87073199481356 27.809319869553264 5 12.01622726022553 37.87277513653687 37.48084554634396 12.630152056893639 6 26.36536874778987 41.05536128246434 18.00715099603159 14.572118973714195 7 25.384071352795566 30.21492279281757 24.623786884601785 19.777218969785075 8 22.378295548308515 37.307964323602214 20.23299673883148 20.08074338925779 9 22.71325291737063 14.66248870378374 23.446033554673686 39.178224824171934 10-11 24.076165965973832 26.58981965345173 28.077973360575225 21.256041019999213 12-13 23.483851322148443 23.089957172606184 27.106989902164948 26.319201603080426 14-15 21.27765117284193 24.823189658559585 23.82617578877058 30.072983379827907 16-17 22.544791953164907 28.15999371341008 25.65468940316687 23.640524930258145 18-19 22.57917174177832 26.829987033908292 26.633039959137168 23.957801265176222 20-21 21.23492200699383 26.88548583552709 26.31232564535775 25.567266512121336 22-23 21.85768732073396 27.707653923225024 26.77645279163883 23.658205964402185 24-25 22.57475148324231 28.267062197949 26.49601194452084 22.662174374287847 26-27 21.662213665474834 28.800931201131586 26.62812856076382 22.90872657262976 28-29 21.261934698047227 27.87955286629209 28.225806451612907 22.632705984047778 30-31 22.669541471847864 27.216022946053197 27.61679305331814 22.497642528780794 32-33 21.753565675219047 28.233173549172918 27.55982083218734 22.45343994342069 34-35 21.559565439471925 28.101056932929946 28.68698675886999 21.652390868728144 36-37 21.57675533377863 27.251385014341285 27.882990845153433 23.28886880672665 38-39 21.77468468822443 27.0735923932262 27.565714510235356 23.586008408314015 40-41 21.220187811873796 26.07314054457585 28.926662999489217 23.780008644061137 42-43 22.215237122313464 26.147793799850692 28.775883069427525 22.861086008408314 44-45 20.112667478684532 27.25776983222663 28.83973124828101 23.789831440807827 46-47 21.803661938627165 25.646340025932187 28.601528427173783 23.948469608266866 48-49 21.22509921024714 26.067738006365172 28.546520765392323 24.16064201799536 50-51 20.074358571372443 25.91450237711681 29.394228124631645 24.6169109268791 52-53 19.569957958429924 26.00929236572237 31.06312129189423 23.35762838395348 54-55 18.724215158539938 26.74845782091077 29.818081804251307 24.709245216297983 56-57 20.322384189226355 25.968036619386268 28.91389336371852 24.795685827668855 58-59 19.059663667439395 26.72193626969471 29.601489135986796 24.6169109268791 60-61 20.140171309575262 27.55048917527799 27.975325134572316 24.334014380574438 62-63 18.71586578130525 28.91634906290519 27.335861066362817 25.031924089426745 64-65 19.206514478802404 29.433519311618404 26.245039487642924 25.11492672193627 66-67 19.24531452595183 29.468390240069155 26.026482260029077 25.25981297394994 68-69 18.986483831676555 29.537149817295983 26.275490157557655 25.200876193469806 70-71 19.644611213704767 29.240501355545952 25.454795489371733 25.66009194137755 72-73 19.659345408824798 29.01408589053475 25.060410199992138 26.266158500648306 74-75 19.909826725865386 28.86870849868374 25.421889120270325 25.799575655180547 76 20.09744214372716 29.03225806451613 25.25735727476327 25.612942516993435 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 2.0 17 2.0 18 5.0 19 7.5 20 19.0 21 31.0 22 36.5 23 54.0 24 76.0 25 86.0 26 195.0 27 279.0 28 353.0 29 452.0 30 602.0 31 888.0 32 1275.5 33 1527.0 34 1792.5 35 2341.5 36 2874.5 37 3124.0 38 3829.5 39 5281.0 40 6415.5 41 6957.0 42 7110.0 43 7176.0 44 7509.0 45 7807.0 46 7838.0 47 7901.5 48 7546.0 49 6648.5 50 6170.0 51 5753.0 52 4704.5 53 3587.5 54 3102.0 55 2960.5 56 2389.5 57 1629.5 58 1299.0 59 1033.0 60 638.0 61 449.0 62 389.0 63 308.5 64 194.5 65 128.0 66 74.0 67 53.0 68 44.0 69 21.0 70 8.0 71 9.0 72 5.5 73 1.5 74 0.5 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 101804.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.082275745550277 #Duplication Level Percentage of deduplicated Percentage of total 1 54.74828556078754 17.017013083965267 2 13.854564990677243 8.612628187497544 3 8.128180008216667 7.579269969745786 4 5.404038807951206 6.718792974735767 5 3.906077173466486 6.070488389454246 6 2.641974528331701 4.927114848139563 7 2.013083462377145 4.379985069348945 8 1.586448819644155 3.9448351734705907 9 1.2135385393293936 3.394758555655966 >10 6.40900041083336 33.1362225452831 >50 0.07900641532092406 1.5166398176888922 >100 0.009480769838510887 0.5893678048013831 >500 0.0031602566128369628 0.6345526698361558 >1k 0.0031602566128369628 1.4783309103768025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 1505 1.4783309103768025 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 646 0.6345526698361558 Illumina PCR Primer Index 10 (95% over 23bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 284 0.27896742760598797 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 204 0.20038505363247022 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 112 0.11001532356292483 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.018663313818710465 0.0 2 0.0 0.0 0.0 0.020627873168048406 0.0 3 0.0 0.0 0.0 0.02259243251738635 0.0 4 0.0 0.0 0.0 0.031432949589407094 0.0 5 0.0 0.0 0.0 0.035362068288082985 0.0 6 0.0 0.0 0.0 0.035362068288082985 0.0 7 0.0 0.0 0.0 0.035362068288082985 0.0 8 0.0 0.0 0.0 0.03732662763742093 0.0 9 0.0 0.0 0.0 0.07956465364818671 0.0 10 0.0 0.0 0.0 0.10215708616557306 0.0 11 0.0 0.0 0.0 0.11296216258693176 0.0 12 0.0 0.0 0.0 0.12769635770696633 0.0 13 0.0 0.0 0.0 0.13850143412832502 0.0 14 0.0 0.0 0.0 0.14439511217633885 0.0 15 0.0 0.0 0.0 0.15127106989902164 0.0 16 0.0 0.0 0.0 0.15814702762170446 0.0 17 0.0 0.0 0.0 0.17091666339240108 0.0 18 0.0 0.0 0.0 0.1777926211150839 0.0 19 0.0 0.0 0.0 0.1984204942831323 0.0 20 0.0 0.0 0.0 0.20726101135515304 0.0 21 0.0 0.0 0.0 0.21806608777651174 0.0 22 0.0 0.0 0.0 0.2219952064751876 0.0 23 0.0 0.0 0.0 0.23672940159522218 0.0 24 0.0 0.0 0.0 0.23869396094456014 0.0 25 0.0 0.0 0.0 0.24556991866724293 0.0 26 0.0 0.0 0.0 0.2612863934619465 0.0 27 0.0 0.0 0.0 0.2819142666299949 0.0 28 0.0 0.0 0.0 0.3153117755687399 0.0 29 0.0 0.0 0.0 0.33790420808612626 0.0 30 0.0 0.0 0.0 0.36639031865152644 0.0 31 0.0 0.0 0.0 0.3929118698675887 0.0 32 0.0 0.0 0.0 0.43711445522769243 0.0 33 0.0 0.0 0.0 0.485246159286472 0.0 34 0.0 0.0 0.0 0.5333778633452516 0.0 35 0.0 0.0 0.0 0.6443754665828455 0.0 36 0.0 0.0 0.0 0.7710895446151428 0.0 37 0.0 0.0 0.0 1.044163294173117 0.0 38 0.0 0.0 0.0 1.4184118502219951 0.0 39 0.0 0.0 0.0 1.8859769753644258 0.0 40 0.0 0.0 0.0 2.3731876940002357 0.0 41 0.0 0.0 0.0 2.9389807866095636 0.0 42 0.0 0.0 0.0 3.55388786295234 0.0 43 0.0 0.0 0.0 4.188440532788496 0.0 44 0.0 0.0 0.0 4.762091862795175 0.0 45 0.0 0.0 0.0 5.511571254567601 0.0 46 0.0 0.0 0.0 6.1981847471612115 0.0 47 0.0 0.0 0.0 6.903461553573534 0.0 48 0.0 0.0 0.0 7.570429452673765 0.0 49 0.0 0.0 0.0 8.19712388511257 0.0 50 0.0 0.0 0.0 8.858198106164787 0.0 51 0.0 0.0 0.0 9.617500294683902 0.0 52 0.0 0.0 0.0 10.32572394012023 0.0 53 0.0 0.0 0.0 10.91902086362029 0.0 54 0.0 0.0 0.0 11.532945660288398 0.0 55 0.0 0.0 0.0 12.106596990295078 0.0 56 0.0 0.0 0.0 12.77258260972064 0.0 57 0.0 0.0 0.0 13.331499744607285 0.0 58 0.0 0.0 0.0 13.94738910062473 0.0 59 0.0 0.0 0.0 14.508270794860712 0.0 60 0.0 0.0 0.0 15.094691760638089 0.0 61 0.0 0.0 0.0 15.739067227220934 0.0 62 0.0 0.0 0.0 16.281285607638207 0.0 63 0.0 0.0 0.0 16.818592589682133 0.0 64 0.0 0.0 0.0 17.372598326195433 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAAAGT 15 0.0022190677 70.00001 4 TAAGTGC 20 7.894012E-5 70.0 43 TACCTCG 25 2.3806634E-4 56.000004 36 AGTTAGA 25 2.3806634E-4 56.000004 36 AGAGATG 25 2.3806634E-4 56.000004 32 TCCGGCT 20 0.0069339415 52.5 34 AGACTCT 20 0.0069339415 52.5 27 AACCGAC 20 0.0069339415 52.5 34 CTGTATT 20 0.0069339415 52.5 59 GTACATG 20 0.0069339415 52.5 1 TACCAGC 20 0.0069339415 52.5 17 CCGGCTG 20 0.0069339415 52.5 35 TACATGC 20 0.0069339415 52.5 2 CGCTCTA 20 0.0069339415 52.5 35 TCTAAGA 20 0.0069339415 52.5 38 CGGGCGC 20 0.0069339415 52.5 12 AGCTCCG 20 0.0069339415 52.5 21 CCTATGG 20 0.0069339415 52.5 2 TTCGCTC 20 0.0069339415 52.5 33 TGAGCGG 20 0.0069339415 52.5 8 >>END_MODULE