FastQCFastQC Report
Wed 25 May 2016
SRR1780601_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780601_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555213
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT7780.14012640193943585No Hit
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT5600.10086219162735743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACA250.002337708835.2428312
GCGTCAT250.00235120235.2015122
CGCGAAA250.002360579835.17296628
AAGACTC300.00568821229.39025518
ATCCCAT602.9580224E-729.3134461
TACATGC602.9744115E-729.294972
CGACAAC407.010729E-427.51110824
TTGTCCG407.06265E-427.47640631
GTCCGGA407.06265E-427.47640633
TGTCCGG407.06265E-427.47640632
GTACATG655.956663E-727.0585691
CGACAAT1200.025.70718217
CGCACCA701.112352E-625.1893716
ATCGGCT701.1264492E-625.15301124
GTCATAC701.137123E-625.1258131
GCGAACT701.138018E-625.12354728
GTATAAA1151.2732926E-1124.8527051
CGGAGAG450.001393976424.45431736
CGGTAAG901.5212208E-824.42567428
TCGTCTT450.001405740924.41907142
Produced by FastQC (version 0.11.5)