FastQCFastQC Report
Wed 25 May 2016
SRR1780601_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780601_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555213
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT108271.9500624084810694No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT39770.7163016716107151No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC20320.3659856667621255No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC17710.3189766810215178No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG9430.16984472625821082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAGC207.847975E-444.0056766
GGCCGCA207.8549405E-443.997757
CCGTCTT4750.036.587644
ATGATTC2500.035.198240
GACGGGG250.002352290835.198213
GCCGTCT5050.034.4140843
ATTGGCG2850.033.96317721
TGGCGTG2950.032.0661623
GCGTGAA2950.032.0661625
TTCAGCC2750.031.99836244
TGAAGGT2900.031.10185428
AGCGGAT2850.030.87561435
GTGAAGG3000.030.79842427
CGCCATT3150.030.72858617
ATTCAGC3000.030.06512843
GTACCTA602.921024E-729.3556271
GGCTTAT602.921024E-729.3556271
CTTATGC454.057061E-529.3424033
GTATCAC300.00573370329.3424033
CTAAACT602.9418516E-729.33183323
Produced by FastQC (version 0.11.5)