##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780600_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7667 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.15690622146863 21.0 12.0 31.0 12.0 34.0 2 20.86096256684492 21.0 11.0 31.0 11.0 34.0 3 20.608060519107866 21.0 11.0 32.0 11.0 34.0 4 21.29320464327638 23.0 11.0 32.0 11.0 34.0 5 20.765488457023608 21.0 11.0 32.0 11.0 34.0 6 23.200730403025954 26.0 11.0 34.0 11.0 37.0 7 23.556410590843875 26.0 11.0 34.0 11.0 37.0 8 23.621755575844528 26.0 11.0 34.0 11.0 37.0 9 23.62853788965697 25.0 12.0 34.0 12.0 37.0 10-11 22.259749576105385 21.5 11.0 34.0 11.0 37.0 12-13 22.011803834615886 21.5 11.0 34.0 11.0 37.0 14-15 21.727990087387504 21.0 11.0 33.5 11.0 37.0 16-17 21.297900091300377 21.0 11.0 31.5 11.0 37.0 18-19 21.597104473718534 21.0 11.0 32.0 11.0 37.0 20-21 21.645819746967522 21.5 11.0 32.0 11.0 37.0 22-23 21.15481935568019 21.0 11.0 32.0 11.0 37.0 24-25 21.27514021129516 21.0 11.0 32.0 10.5 37.0 26-27 20.971240380853004 21.0 11.0 31.0 10.5 37.0 28-29 20.559540889526545 21.0 11.0 31.0 11.0 37.0 30-31 20.486761445154556 21.0 11.0 31.0 11.0 37.0 32-33 20.725316290596062 21.0 11.0 31.0 11.0 37.0 34-35 21.05673666362332 21.0 11.0 31.0 11.0 37.0 36-37 20.869375244554583 21.0 11.0 31.0 11.0 37.0 38-39 20.267705751923828 16.0 11.0 29.0 11.0 37.0 40-41 20.64027650971697 21.0 11.0 27.5 11.0 36.0 42-43 20.942480761706012 21.0 11.0 31.0 11.0 37.0 44-45 20.578322681622538 21.0 11.0 29.0 11.0 36.0 46-47 20.733794182861615 21.0 11.0 31.0 11.0 36.5 48-49 20.953762879874787 21.0 11.0 31.0 11.0 36.5 50-51 21.161601669492633 21.0 11.0 31.0 11.0 36.5 52-53 21.204708490935175 21.0 11.0 31.0 11.0 36.0 54-55 21.017673144645883 21.0 11.0 31.0 11.0 36.0 56-57 20.434785444111128 20.5 11.0 29.0 11.0 36.0 58-59 20.597626190165645 20.0 11.0 29.0 11.0 36.0 60-61 20.81583409416982 21.0 11.0 31.0 11.0 36.0 62-63 20.210512586409287 19.5 11.0 28.5 11.0 36.0 64-65 20.520673014216776 20.0 11.0 29.5 11.0 36.0 66-67 20.93406808399635 20.5 11.0 31.0 11.0 36.0 68-69 20.431720359984347 19.0 11.0 30.0 11.0 36.0 70-71 21.031694274161993 21.0 11.0 31.0 11.0 36.0 72-73 20.812116864484153 20.0 11.0 30.0 11.0 36.0 74-75 20.524520673014216 20.0 11.0 29.5 11.0 36.0 76 19.45298030520412 19.0 11.0 26.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.1411708162529557 1101 2 0.9840778067894256 1101 3 -1.618343958563802 1101 4 -0.7733110376366348 1101 5 -1.3868651246150918 1101 6 -0.26461753016784684 1101 7 -2.1985720132180013 1101 8 -2.7464286500953534 1101 9 1.0843125393289164 1101 10-11 -1.8590809106965018 1101 12-13 -1.712560588814231 1101 14-15 -1.303872380338376 1101 16-17 -0.36268769571558934 1101 18-19 -1.5472603598974466 1101 20-21 -2.968662327951666 1101 22-23 -0.46397465349757283 1101 24-25 -3.6347048318842177 1101 26-27 -2.326556640132395 1101 28-29 -0.10269657769585194 1101 30-31 -0.14108974962087828 1101 32-33 -1.1963265465119122 1101 34-35 -0.7506256188109113 1101 36-37 -1.6235822042157046 1101 38-39 -3.248803163588036 1101 40-41 -2.4655943521546 1101 42-43 -5.356394164637049 1101 44-45 -1.2410802961562872 1101 46-47 -3.0035160984902802 1101 48-49 -1.957870709651779 1101 50-51 -1.7860420892898148 1101 52-53 -2.2751928448378145 1101 54-55 0.9150407144534825 1101 56-57 -1.5200729583683064 1101 58-59 -3.294878793759633 1101 60-61 -2.405416602593359 1101 62-63 -2.487030816682527 1101 64-65 -5.315110829067155 1101 66-67 -2.0226001309331743 1101 68-69 -2.879035875773706 1101 70-71 -3.0714310921011503 1101 72-73 -3.4820417044487044 1101 74-75 -2.5827922031790393 1101 76 -2.491186461142803 1102 1 -0.45111233672079365 1102 2 2.6010368711169107 1102 3 1.434287620383568 1102 4 1.3582679097317865 1102 5 0.07219920287028714 1102 6 1.092107616030983 1102 7 -0.1342445278378861 1102 8 -0.14408946880880436 1102 9 1.1106283288026013 1102 10-11 1.3909190893034982 1102 12-13 -0.39092316191364773 1102 14-15 0.399343993930632 1102 16-17 -0.9679508536103256 1102 18-19 0.9483536751902726 1102 20-21 -0.03883776654815563 1102 22-23 1.8415809020579843 1102 24-25 -1.1902603874397748 1102 26-27 -1.3060888038750846 1102 28-29 -0.6070825426081328 1102 30-31 0.49048919774754296 1102 32-33 -2.670010757038227 1102 34-35 -1.471385852729039 1102 36-37 -1.9174418533385094 1102 38-39 1.3272202282248244 1102 40-41 1.486160033810311 1102 42-43 -2.6034701880288615 1102 44-45 -0.799559828320028 1102 46-47 -0.10585527977682574 1102 48-49 1.8827725652020213 1102 50-51 -0.9570947208687635 1102 52-53 2.4981989680276797 1102 54-55 -0.8758949580611386 1102 56-57 -2.2686109700642128 1102 58-59 -2.4498495539935483 1102 60-61 -1.3615569534705507 1102 62-63 1.1957177213291672 1102 64-65 0.2638365393538997 1102 66-67 0.7712595181896305 1102 68-69 -2.0544744722649355 1102 70-71 -1.2103199809900396 1102 72-73 -2.395784394507185 1102 74-75 -1.395657700255061 1102 76 -2.4882624845346157 1103 1 1.6972130221308817 1103 2 0.4790273017389204 1103 3 0.5180196777998347 1103 4 0.40850714418154865 1103 5 2.0626298248798562 1103 6 1.285887520337205 1103 7 0.2963774817314935 1103 8 2.516197612530906 1103 9 2.6752216302380063 1103 10-11 1.5272827256671313 1103 12-13 -1.328722204975847 1103 14-15 0.2845114580454613 1103 16-17 0.04640321337532072 1103 18-19 0.34667903404194433 1103 20-21 1.0843679750786386 1103 22-23 0.227944538421621 1103 24-25 0.8324668852874986 1103 26-27 -1.596758660334416 1103 28-29 -1.5269390019382811 1103 30-31 0.7680011594700282 1103 32-33 -0.9463265465119122 1103 34-35 0.691293573108279 1103 36-37 -1.59327917391267 1103 38-39 -1.8270859918708666 1103 40-41 0.27682989026963867 1103 42-43 -1.0281113363542183 1103 44-45 -1.9431004981764914 1103 46-47 -1.9934150883892698 1103 48-49 0.03707878529771591 1103 50-51 1.9866851834374586 1103 52-53 0.12379705415208164 1103 54-55 1.8418083912211571 1103 56-57 -1.446840635135981 1103 58-59 -2.148414147294986 1103 60-61 0.7006440034672501 1103 62-63 -2.0148085944603054 1103 64-65 -2.0196562836126084 1103 66-67 -0.3483577066907486 1103 68-69 -3.485096481834315 1103 70-71 1.130589109919054 1103 72-73 0.6795744571674582 1103 74-75 -1.6181457385325757 1103 76 0.7461872762309376 1104 1 -0.3217263718085128 1104 2 -2.168699970988353 1104 3 -0.618343958563802 1104 4 -2.106644370969967 1104 5 -3.5813095690595382 1104 6 0.8881602476099317 1104 7 0.6486502090042201 1104 8 -2.4269842056509106 1104 9 -3.4573541273377515 1104 10-11 -2.004914244029834 1104 12-13 -3.018116144369788 1104 14-15 -2.8802612692272653 1104 16-17 -2.2376876957155893 1104 18-19 -3.8805936932307823 1104 20-21 -3.593662327951666 1104 22-23 -2.4292524312753514 1104 24-25 -1.7110937207731105 1104 26-27 -3.812667751243506 1104 28-29 -4.831863244362522 1104 30-31 -2.224423082954214 1104 32-33 -3.508826546511912 1104 34-35 -3.736736729922022 1104 36-37 -3.311082204215701 1104 38-39 -0.7418587191435932 1104 40-41 -0.9655943521546 1104 42-43 -3.280005275748156 1104 44-45 -1.477191407267398 1104 46-47 -1.447960542934723 1104 48-49 -0.7773151540962218 1104 50-51 -2.140208755956479 1104 52-53 -2.9765817337267073 1104 54-55 -1.8627370633242961 1104 56-57 -2.82562851392386 1104 58-59 -1.4893232382040757 1104 60-61 -2.0304166025933554 1104 62-63 0.006024738873030344 1104 64-65 -1.4609441624004873 1104 66-67 -1.939266797599842 1104 68-69 -4.12903587577371 1104 70-71 -2.5436533143233717 1104 72-73 -2.405652815559815 1104 74-75 -2.44390331429015 1104 76 -0.4356309055872458 1105 1 2.274288120945112 1105 2 0.7932565507507761 1105 3 3.077308215349241 1105 4 -1.7515719072018534 1105 5 1.2484005758680006 1105 6 0.4262037258708027 1105 7 0.6758241220476968 1105 8 0.7414940552186557 1105 9 0.2799647132419594 1105 10-11 2.6083103936513226 1105 12-13 1.2028983483838367 1105 14-15 -0.09493518227074205 1105 16-17 2.0503557825452816 1105 18-19 3.717232393725741 1105 20-21 -0.4541695743284748 1105 22-23 2.3415809020579843 1105 24-25 1.244522221255874 1105 26-27 0.19276703136518947 1105 28-29 1.0096222628838554 1105 30-31 2.4241276416834694 1105 32-33 2.803673453488088 1105 34-35 -0.9233309328205728 1105 36-37 0.48873663636400977 1105 38-39 1.630423889552059 1105 40-41 -0.9909566709951818 1105 42-43 -0.46388208734235903 1105 44-45 0.1043303318630393 1105 46-47 1.052039457065277 1105 48-49 -1.7030397917773854 1105 50-51 0.7683057367971386 1105 52-53 -0.3850962264803286 1105 54-55 -0.5909979328895147 1105 56-57 0.3229222107138199 1105 58-59 4.912850674839401 1105 60-61 2.558351513348672 1105 62-63 1.0721479272788272 1105 64-65 -0.6629369160236784 1105 66-67 -1.65665810194767 1105 68-69 1.4832829648060013 1105 70-71 2.2896800190099604 1105 72-73 0.04815153226627089 1105 74-75 2.75537204802869 1105 76 0.6078473552823205 1106 1 1.398511723429582 1106 2 -0.34131901860740044 1106 3 -2.713582053801897 1106 4 2.9647842004586025 1106 5 -0.42654766429763313 1106 6 -0.29636356191387847 1106 7 2.1665073518613625 1106 8 0.17420627053956395 1106 9 2.0128839679003434 1106 10-11 0.5099667083511186 1106 12-13 3.3985505222968797 1106 14-15 1.8667625402965449 1106 16-17 -0.6245924576203521 1106 18-19 1.2979777353406483 1106 20-21 0.43609957681023914 1106 22-23 1.1511047115817945 1106 24-25 0.8335491363697507 1106 26-27 -1.0567153702911263 1106 28-29 -0.44793467293394684 1106 30-31 2.263672155141027 1106 32-33 3.1846258344404674 1106 34-35 1.5073108891255984 1106 36-37 -0.2307250613585623 1106 38-39 0.8146888999040272 1106 40-41 0.42726279070254236 1106 42-43 0.06820900996612878 1106 44-45 1.4335228784468868 1106 46-47 1.1472775523033718 1106 48-49 1.633399131618063 1106 50-51 -0.059851613099340284 1106 52-53 0.9668706472256758 1106 54-55 0.3872629366757039 1106 56-57 1.6624667241713773 1106 58-59 0.45115295227211405 1106 60-61 -0.14351184068859268 1106 62-63 -0.014808594460305358 1106 64-65 -0.19606321001953475 1106 66-67 -1.3678382261712727 1106 68-69 0.7400117432739108 1106 70-71 1.0753943047242451 1106 72-73 -1.1050575774645743 1106 74-75 2.0680014476146127 1106 76 -3.7213451913015323 1107 1 1.9199402948581543 1107 2 0.6063000290116456 1107 3 1.6816560414361987 1107 4 -0.2733110376366348 1107 5 -0.564642902392869 1107 6 -3.2368397523900683 1107 7 -1.22634979099578 1107 8 0.39801579434908874 1107 9 2.384312539328917 1107 10-11 2.2909190893034967 1107 12-13 -1.4264494777031196 1107 14-15 2.232238730772732 1107 16-17 -2.2376876957155893 1107 18-19 -1.4805936932307837 1107 20-21 0.3980043387150012 1107 22-23 -2.758419097942017 1107 24-25 -0.2902603874397762 1107 26-27 -1.6376677512435052 1107 28-29 -0.6610299110291891 1107 30-31 -0.09108974962087757 1107 32-33 0.05367345348808783 1107 34-35 -0.4700700632553527 1107 36-37 1.1930844624509618 1107 38-39 -2.1043587191435904 1107 40-41 -0.307261018821265 1107 42-43 0.6991613909185084 1107 44-45 1.5478085927326006 1107 46-47 -1.522960542934726 1107 48-49 -0.6106484874295575 1107 50-51 -0.11104208928981762 1107 52-53 -0.28074840039337445 1107 54-55 1.1372629366757039 1107 56-57 -0.8422951805905292 1107 58-59 -1.2143232382040772 1107 60-61 1.8529167307399774 1107 62-63 -0.03980859446030394 1107 64-65 -0.14011082906715444 1107 66-67 0.5357332024001593 1107 68-69 0.012630790892959709 1107 70-71 1.6146800190099597 1107 72-73 0.08184718444018557 1107 74-75 -1.0772366476234794 1107 76 -0.0856309055872444 1108 1 0.12994029485815517 1108 2 -2.253699970988354 1108 3 -3.658343958563801 1108 4 -0.47331103763663407 1108 5 0.37535709760712876 1108 6 -3.6068397523900693 1108 7 -2.29634979099578 1108 8 -2.621984205650911 1108 9 1.504312539328918 1108 10-11 -1.6090809106965018 1108 12-13 -1.7214494777031177 1108 14-15 -1.3027612692272648 1108 16-17 -2.0226876957155895 1108 18-19 -0.9905936932307817 1108 20-21 -4.141995661284998 1108 22-23 -1.8784190979420146 1108 24-25 -1.4502603874397764 1108 26-27 -0.977667751243505 1108 28-29 -3.126029911029189 1108 30-31 -1.1210897496208787 1108 32-33 -0.7763265465119105 1108 34-35 -1.2250700632553553 1108 36-37 -1.0069155375490375 1108 38-39 -1.604358719143594 1108 40-41 -2.082261018821267 1108 42-43 -1.3308386090814928 1108 44-45 -2.2121914072673974 1108 46-47 -2.7679605429347234 1108 48-49 -1.885648487429556 1108 50-51 -0.8960420892898142 1108 52-53 -1.2607484003933713 1108 54-55 -1.7927370633242958 1108 56-57 -1.3622951805905252 1108 58-59 -3.3593232382040767 1108 60-61 0.627916730739976 1108 62-63 -1.1148085944603068 1108 64-65 -0.8951108290671534 1108 66-67 -1.7592667975998424 1108 68-69 -2.4723692091070397 1108 70-71 -0.41031998099003886 1108 72-73 -3.023152815559815 1108 74-75 -2.1872366476234824 1108 76 -2.595630905587246 1109 1 3.2099402948581535 1109 2 0.3463000290116476 1109 3 3.4616560414361963 1109 4 1.0466889623633655 1109 5 1.9753570976071302 1109 6 6.1931602476099314 1109 7 0.6236502090042215 1109 8 -2.4619842056509107 1109 9 2.7043125393289174 1109 10-11 1.1309190893035002 1109 12-13 2.498550522296881 1109 14-15 0.3972387307727345 1109 16-17 0.43731230428441137 1109 18-19 -0.07059369323078002 1109 20-21 -0.6819956612850007 1109 22-23 2.681580902057984 1109 24-25 1.8097396125602252 1109 26-27 -2.197667751243504 1109 28-29 -0.006029911029187929 1109 30-31 -0.2610897496208757 1109 32-33 1.5436734534880898 1109 34-35 -0.2450700632553513 1109 36-37 -0.7269155375490364 1109 38-39 -0.02435871914359211 1109 40-41 -0.48226101882126926 1109 42-43 1.0491613909185098 1109 44-45 -0.7921914072673957 1109 46-47 -0.7079605429347211 1109 48-49 -3.7656484874295586 1109 50-51 -2.9560420892898165 1109 52-53 -0.2607484003933713 1109 54-55 -0.3727370633242941 1109 56-57 2.137704819409471 1109 58-59 1.4006767617959248 1109 60-61 -1.3920832692600236 1109 62-63 1.6851914055396975 1109 64-65 0.344889170932845 1109 66-67 -1.939266797599842 1109 68-69 -1.7123692091070417 1109 70-71 0.7496800190099613 1109 72-73 0.8968471844401869 1109 74-75 -0.08723664762348449 1109 76 0.7643690944127535 1110 1 -0.12096879605093491 1110 2 -0.20279088007926305 1110 3 -0.43652577674561854 1110 4 0.18123441690881847 1110 5 0.19899346124349293 1110 6 -0.25956702511734164 1110 7 0.43274111809513016 1110 8 3.6980157943490894 1110 9 -2.0065965515801736 1110 10-11 -0.47271727433286514 1110 12-13 0.4212777950241531 1110 14-15 0.011784185318187923 1110 16-17 1.4327668497389574 1110 18-19 0.8466790340419443 1110 20-21 1.5843679750786386 1110 22-23 -0.4084190979420157 1110 24-25 0.5597396125602252 1110 26-27 1.6986958851201273 1110 28-29 2.3139700889708124 1110 30-31 -0.936544295075425 1110 32-33 -1.0145083646937323 1110 34-35 -1.4223427905280808 1110 36-37 2.293084462450963 1110 38-39 -2.5543587191435932 1110 40-41 0.07228443572418541 1110 42-43 2.835525027282145 1110 44-45 2.1705358654598754 1110 46-47 -0.3570514520256296 1110 48-49 0.26435151257044254 1110 50-51 -1.149678452926178 1110 52-53 1.6692515996066284 1110 54-55 2.4099902094029773 1110 56-57 -0.3786588169541645 1110 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2116 70-71 2.0543859013629024 2116 72-73 -0.01962340379510863 2116 74-75 1.0021751170824018 2116 76 0.8584867414715767 2117 1 -0.842559705141845 2117 2 -1.356199970988353 2117 3 1.256656041436198 2117 4 -1.7733110376366348 2117 5 -0.10214290239287038 2117 6 3.1381602476099317 2117 7 0.4611502090042201 2117 8 6.4480157943490894 2117 9 -0.5406874606710836 2117 10-11 3.234669089303498 2117 12-13 5.83605052229688 2117 14-15 -3.3802612692272653 2117 16-17 -2.3626876957155893 2117 18-19 0.3694063067692177 2117 20-21 -3.270745661284998 2117 22-23 -0.2521690979420157 2117 24-25 -3.159010387439775 2117 26-27 -4.375167751243506 2117 28-29 -3.6547799110291876 2117 30-31 -2.7348397496208783 2117 32-33 -0.16507654651191217 2117 34-35 1.6799299367446459 2117 36-37 -1.9256655375490368 2117 38-39 -0.33560871914359325 2117 40-41 -0.8510110188212678 2117 42-43 -0.4258386090814916 2117 44-45 -0.914691407267398 2117 46-47 -0.2292105429347231 2117 48-49 -2.0168984874295575 2117 50-51 0.24520791071018522 2117 52-53 -0.01824840039337161 2117 54-55 0.23101293667570388 2117 56-57 1.7264548194094722 2117 58-59 2.2606767617959243 2117 60-61 1.4904167307399767 2117 62-63 -0.07730859446030536 2117 64-65 -0.15886082906715515 2117 66-67 0.9044832024001579 2117 68-69 1.7563807908929583 2117 70-71 1.1021800190099604 2117 72-73 1.1880971844401849 2117 74-75 -0.9022366476234822 2117 76 -0.18563090558724582 2118 1 -2.912412646318316 2118 2 -3.528994088635411 2118 3 -4.618343958563802 2118 4 -0.6262522141072218 2118 5 0.4530041564306586 2118 6 1.3955131887864027 2118 7 -0.4469380262898959 2118 8 -0.30198420565091055 2118 9 -2.474510990082848 2118 10-11 1.0673896775387917 2118 12-13 -0.5426259482913558 2118 14-15 -1.0015847986390298 2118 16-17 0.8431946572255846 2118 18-19 0.8399945420633337 2118 20-21 -0.4490544848144111 2118 22-23 -1.0701838038243707 2118 24-25 -2.454966269792717 2118 26-27 0.44468518993296513 2118 28-29 1.6963230301472834 2118 30-31 -1.6999132790326428 2118 32-33 -1.9610324288648542 2118 34-35 -1.4156582985494701 2118 36-37 -1.6480920081372723 2118 38-39 -1.4808293073788903 2118 40-41 0.1765625105905002 2118 42-43 1.8021025673890954 2118 44-45 -0.17572081903210446 2118 46-47 -3.095019366464136 2118 48-49 1.073175041982207 2118 50-51 0.5816049695337142 2118 52-53 0.09572218784192188 2118 54-55 0.44608646608747193 2118 56-57 -0.6540598864728793 2118 58-59 -1.3863820617334888 2118 60-61 -1.3956126810247298 2118 62-63 -0.7795144768132474 2118 64-65 -3.1386402408318617 2118 66-67 1.4136743788707449 2118 68-69 -2.0653103855776287 2118 70-71 1.613209430774667 2118 72-73 0.21567071385194936 2118 74-75 -3.0860601770352467 2118 76 -2.96504267029313 2119 1 2.9032736281914886 2119 2 2.4396333623449813 2119 3 1.5816560414361973 2119 4 0.2600222956967002 2119 5 0.40202376427379605 2119 6 -0.5535064190567347 2119 7 3.9569835423375537 2119 8 -1.3019842056509106 2119 9 2.0509792059955814 2119 10-11 3.224252422636834 2119 12-13 3.265217188963547 2119 14-15 2.4905720641060682 2119 16-17 1.9373123042844114 2119 18-19 2.7694063067692163 2119 20-21 1.0980043387150005 2119 22-23 0.008247568724648602 2119 24-25 5.276406279226894 2119 26-27 1.3956655820898298 2119 28-29 4.213970088970811 2119 30-31 3.0255769170457896 2119 32-33 1.8036734534880878 2119 34-35 1.4549299367446444 2119 36-37 3.059751129117629 2119 38-39 2.9956412808564075 2119 40-41 3.9510723145120643 2119 42-43 2.915828057585177 2119 44-45 3.2478085927326035 2119 46-47 5.318706123731943 2119 48-49 6.18101817923711 2119 50-51 0.3639579107101838 2119 52-53 1.9192515996066284 2119 54-55 4.387262936675704 2119 56-57 1.457704819409475 2119 58-59 2.194010095129258 2119 60-61 0.7945833974066439 2119 62-63 3.6185247388730275 2119 64-65 3.2182225042661763 2119 66-67 1.494066535733488 2119 68-69 3.054297457559624 2119 70-71 1.8230133523432919 2119 72-73 1.5901805177735184 2119 74-75 0.8394300190431849 2119 76 2.031035761079419 >>END_MODULE >>Per sequence quality scores fail #Quality Count 12 10.0 13 92.0 14 256.0 15 452.0 16 692.0 17 819.0 18 935.0 19 938.0 20 793.0 21 599.0 22 439.0 23 258.0 24 186.0 25 145.0 26 111.0 27 75.0 28 53.0 29 59.0 30 50.0 31 58.0 32 54.0 33 55.0 34 83.0 35 109.0 36 170.0 37 176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.51077246452969 26.97057277982133 9.104046242774565 37.41460851287441 2 5.88235294117647 22.968566584061563 25.981479066127562 45.167601408634404 3 5.986696230598669 25.564105908438762 38.411373418547015 30.037824442415545 4 3.8346158862658144 44.345898004434595 21.103430285639753 30.716055823659843 5 4.460675622799009 33.40289552628147 36.311464718925265 25.82496413199426 6 7.773575061953827 31.21168644841529 12.690752575974958 48.323985913655925 7 5.334550671709925 47.43706795356724 12.442937263597235 34.7854441111256 8 5.751923829398722 36.06364940654754 12.899439154819357 45.28498760923438 9 10.003912873353332 28.850919525238034 12.312508151819486 48.83265944958915 10-11 8.027911829920438 30.62475544541542 23.74461979913917 37.602712925524976 12-13 7.447502282509457 26.54884570236077 24.1424285900613 41.86122342506848 14-15 12.449458719186122 30.853006391026476 18.64484152862919 38.05269336115821 16-17 16.766662319029606 30.807356201904263 17.914438502673796 34.51154297639233 18-19 12.775531498630494 27.28577018390505 17.60793008999609 42.33076822746837 20-21 11.066910134341985 28.029216121038214 19.929568279640016 40.974305464979786 22-23 17.992695969740446 28.727011869049175 15.905830181296466 37.37446197991392 24-25 13.532020346941437 34.602843354636754 15.397156645363244 36.46797965305856 26-27 11.686448415286293 34.56371462110343 19.707838789617842 34.04199817399243 28-29 14.673275074996738 30.455197600104345 23.35985391939481 31.51167340550411 30-31 13.642885091952525 28.93569844789357 25.472805530194336 31.948610929959564 32-33 10.551715142819877 35.09195252380331 22.59684361549498 31.75948871788183 34-35 10.06260597365332 36.43537237511413 20.105647580539976 33.39637407069258 36-37 12.090778661797314 30.8921351245598 21.964262423372897 35.05282379026999 38-39 12.736402765097168 25.485848441372116 27.55314986304943 34.22459893048128 40-41 10.734315899308726 26.74448937002739 26.053215077605323 36.46797965305856 42-43 7.088822225120647 24.06417112299465 28.850919525238034 39.99608712664667 44-45 8.060519107864875 24.905438893961133 31.68775270640407 35.34628929176992 46-47 8.301812964653712 29.78348767444894 27.083605060649536 34.83109430024782 48-49 8.295291509064823 32.37250554323725 21.977305334550675 37.35489761314725 50-51 8.24964131994261 32.144254597626194 19.46654493282901 40.13955914960219 52-53 6.508412677709664 33.585496282770315 20.803443328550934 39.10264771096909 54-55 7.088822225120647 30.36389722185992 20.816486239728707 41.73079431329072 56-57 11.895134994130691 30.983435502804223 19.596974044606757 37.52445545845833 58-59 10.714751532542063 29.613929829137863 18.7426633624625 40.92865527585757 60-61 7.851832529020478 27.69662188600496 20.353462892917698 44.09808269205687 62-63 10.695187165775401 28.720490413460286 18.683970262162514 41.9003521586018 64-65 10.225642363375504 29.894352419460024 15.51454284596322 44.365462371201254 66-67 8.112690752575975 36.35711490804748 13.88417894874136 41.646015390635185 68-69 9.690883005086736 36.63753749836964 13.225511934263729 40.4460675622799 70-71 7.4018520933872445 40.2895526281466 12.808138776574932 39.50045650189122 72-73 7.956175818442676 40.550410851702104 13.995043693752447 37.49836963610278 74-75 8.738750489109169 41.4308073562019 13.792878570496935 36.037563584191986 76 10.616929698708752 39.44176340159123 12.938567888352681 37.00273901134734 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.0 11 0.0 12 0.0 13 1.0 14 1.0 15 0.5 16 2.0 17 3.0 18 3.5 19 3.5 20 6.0 21 9.0 22 12.5 23 14.5 24 16.5 25 20.0 26 25.0 27 33.0 28 42.0 29 48.0 30 47.0 31 64.5 32 89.5 33 96.0 34 101.0 35 124.5 36 169.5 37 196.0 38 213.5 39 276.0 40 325.5 41 332.5 42 335.0 43 374.0 44 437.5 45 464.0 46 466.0 47 472.0 48 470.0 49 463.5 50 465.0 51 453.0 52 407.5 53 366.5 54 359.0 55 306.5 56 244.5 57 231.5 58 228.0 59 214.0 60 183.0 61 146.0 62 126.0 63 117.0 64 99.0 65 78.0 66 60.0 67 54.0 68 43.0 69 30.5 70 22.0 71 15.0 72 15.0 73 12.0 74 9.0 75 9.0 76 5.5 77 2.0 78 3.5 79 5.0 80 5.0 81 3.5 82 2.0 83 2.0 84 1.5 85 1.0 86 1.0 87 1.0 88 1.0 89 1.0 90 2.0 91 2.5 92 2.0 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 1.0 99 3.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7173601147776184 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 7667.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.23059866962306 #Duplication Level Percentage of deduplicated Percentage of total 1 97.98048251558687 94.2872049041346 2 1.0707508809975603 2.0607799660884307 3 0.5692599620493358 1.6434068083996347 4 0.1626457034426674 0.6260597365331942 5 0.10843046896177824 0.5217164471109952 6 0.02710761724044456 0.15651493413329856 7 0.05421523448088912 0.36520151297769665 8 0.0 0.0 9 0.01355380862022228 0.11738620059997393 >10 0.01355380862022228 0.22172949002217296 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG 17 0.22172949002217296 No Hit CTGTCTCTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATC 9 0.11738620059997393 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1304291117777488 0.0 2 0.0 0.0 0.0 0.1304291117777488 0.0 3 0.0 0.0 0.0 0.1304291117777488 0.0 4 0.0 0.0 0.0 0.1304291117777488 0.0 5 0.0 0.0 0.0 0.15651493413329856 0.0 6 0.0 0.0 0.0 0.15651493413329856 0.0 7 0.0 0.0 0.0 0.15651493413329856 0.0 8 0.0 0.0 0.0 0.15651493413329856 0.0 9 0.0 0.0 0.0 0.15651493413329856 0.0 10 0.0 0.0 0.0 0.16955784531107343 0.0 11 0.0 0.0 0.0 0.1826007564888483 0.0 12 0.0 0.0 0.0 0.22172949002217296 0.0 13 0.0 0.0 0.0 0.23477240119994783 0.0 14 0.0 0.0 0.0 0.2478153123777227 0.0 15 0.0 0.0 0.0 0.2999869570888222 0.0 16 0.0 0.0 0.0 0.32607277944437196 0.0 17 0.0 0.0 0.0 0.46954480239989566 0.0 18 0.0 0.0 0.0 0.6130168253554193 0.0 19 0.0 0.0 0.0 0.8738750489109169 0.0 20 0.0 0.0 0.0 0.9651754271553411 0.0 21 0.0 0.0 0.0 0.978218338333116 0.0 22 0.0 0.0 0.0 1.0303899830442154 0.0 23 0.0 0.0 0.0 1.082561627755315 0.0 24 0.0 0.0 0.0 1.1347332724664145 0.0 25 0.0 0.0 0.0 1.1347332724664145 0.0 26 0.0 0.0 0.0 1.1347332724664145 0.0 27 0.0 0.0 0.0 1.1347332724664145 0.0 28 0.0 0.0 0.0 1.1477761836441893 0.0 29 0.0 0.0 0.0 1.1477761836441893 0.0 30 0.0 0.0 0.0 1.173862005999739 0.0 31 0.0 0.0 0.0 1.2782052954219383 0.0 32 0.0 0.0 0.0 1.3303769401330376 0.0 33 0.0 0.0 0.0 1.4347202295552368 0.0 34 0.0 0.0 0.0 1.526020607799661 0.0 35 0.0 0.0 0.0 1.6955784531107343 0.0 36 0.0 0.0 0.0 1.8651362984218078 0.0 37 0.0 0.0 0.0 2.0868657884439807 0.0 38 0.0 0.0 0.0 2.399895656710578 0.0 39 0.0 0.0 0.0 2.6607538802660753 0.0 40 0.0 0.0 0.0 2.8303117255771486 0.0 41 0.0 0.0 0.0 3.0650841267770965 0.0 42 0.0 0.0 0.0 3.365071083865919 0.0 43 0.0 0.0 0.0 3.5476718403547673 0.0 44 0.0 0.0 0.0 3.8346158862658144 0.0 45 0.0 0.0 0.0 3.9520020868657886 0.0 46 0.0 0.0 0.0 4.004173731576888 0.0 47 0.0 0.0 0.0 4.1737315768879615 0.0 48 0.0 0.0 0.0 4.369375244554584 0.0 49 0.0 0.0 0.0 4.525890178687884 0.0 50 0.0 0.0 0.0 4.656319290465632 0.0 51 0.0 0.0 0.0 4.799791313421156 0.0 52 0.0 0.0 0.0 5.060649536976653 0.0 53 0.0 0.0 0.0 5.373679405243251 0.0 54 0.0 0.0 0.0 5.5432372505543235 0.0 55 0.0 0.0 0.0 5.5954088952654235 0.0 56 0.0 0.0 0.0 5.764966740576496 0.0 57 0.0 0.0 0.0 5.895395852354246 0.0 58 0.0 0.0 0.0 6.104082431198644 0.0 59 0.0 0.0 0.0 6.234511542976392 0.0 60 0.0 0.0 0.0 6.299726098865267 0.0 61 0.0 0.0 0.0 6.338854832398591 0.0 62 0.0 0.0 0.0 6.677970523020738 0.0 63 0.0 0.0 0.0 6.743185078909613 0.0 64 0.0 0.0 0.0 6.9388287465762355 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATCTC 15 0.0021411483 70.0 7 TCTCGGT 20 0.006692288 52.5 43 TATCACC 45 0.0040488667 31.111109 3 TCTGACG 70 0.001118723 25.0 11 CTGACGC 80 0.002434901 21.875 12 >>END_MODULE