FastQCFastQC Report
Wed 25 May 2016
SRR1780600_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780600_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7667
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT296338.64614581974697No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT6828.895265423242469RNA PCR Primer, Index 24 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG4565.947567497065346No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC2643.443328550932568No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT1161.512977696621886No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1121.4608060519107866RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT941.2260336507108387No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT941.2260336507108387No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT771.0043041606886656RNA PCR Primer, Index 24 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC560.7304030259553933No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC390.5086735359332203No Hit
ACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCT310.4043302465110213RNA PCR Primer, Index 24 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG310.4043302465110213No Hit
CTTATACACATCTCCGAGCCCACGAGCCGTGATAGCATCTCGTATGCCGT300.3912873353332464No Hit
CTTATACACATCTCCGAGCCCACGCGACGTGATAGCATCTCGTATGCCGT290.3782444241554715No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCG280.36520151297769665No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGTCTT260.33911569062214686No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTAGTATGCCGT220.2869440459110474No Hit
ATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTT210.2739011347332725RNA PCR Primer, Index 34 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC170.22172949002217296No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGATAGCATCTCGTATGCCGT170.22172949002217296No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCC170.22172949002217296No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCACGTATGCCGT160.20868657884439806No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT160.20868657884439806No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATCGCATCTCGTATGCCGT160.20868657884439806No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCG160.20868657884439806No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCCCGTATGCCGT160.20868657884439806No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGTCTT150.1956436676666232No Hit
CTTATACACATCTCCGAGCACACGAGACGTGATAGCATCTCGTATGCCGT150.1956436676666232No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT140.18260075648884833No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCC140.18260075648884833No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC130.16955784531107343No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATTTCGTATGCCGT130.16955784531107343No Hit
CTTATACACATATCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT130.16955784531107343No Hit
ATACACATCTCCGAGCCCACGAGCCGTGATAGCATCTCGTATGCCGTCTT120.15651493413329856RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCCTCTCGTATGCCGT120.15651493413329856No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTA120.15651493413329856No Hit
CTTATACACATCTCCGCGCCCACGAGACGTGATAGCATCTCGTATGCCGT110.1434720229555237No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCC110.1434720229555237No Hit
CTTATACACCTCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT110.1434720229555237No Hit
CTTAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC110.1434720229555237RNA PCR Primer, Index 24 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGTCTT110.1434720229555237No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAAATCGTATGCCGT110.1434720229555237No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG110.1434720229555237No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGC90.11738620059997393No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT90.11738620059997393No Hit
CTTATACACATCTCCGAGCCCAAGAGACGTGATAGCATCTCGTATGCCGT90.11738620059997393No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT90.11738620059997393No Hit
CTTATACACATCTCCGAGCCCACGTGACGTGATAGCATCTCGTATGCCGT90.11738620059997393No Hit
CTTATACACATCTCCGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC80.10434328942219903No Hit
CTTATCACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC80.10434328942219903No Hit
CTTATACACATCTCCGAGCCAACGAGACGTGATAGCATCTCGTATGCCGT80.10434328942219903No Hit
CTTATACACATCTACGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT80.10434328942219903No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAG80.10434328942219903No Hit
CTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTTGA80.10434328942219903RNA PCR Primer, Index 24 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTCTGCCGT80.10434328942219903No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG80.10434328942219903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGTA252.580391E-670.038
TAGCACC207.4367985E-570.033
CACCTCG252.580391E-670.036
ATAGCAC207.4367985E-570.032
ACCTCGT252.580391E-670.037
AGCACCT207.4367985E-570.034
GCACCTC207.4367985E-570.035
CTTATAC5650.055.1327441
TTATACA5600.054.3752
TATACAC5750.052.956523
TTGCAAA200.00669228852.558
AACAACC200.00669228852.566
TGCAAAA200.00669228852.559
TATCGTA200.00669228852.538
GAAAAAC200.00669228852.560
TAGCATA200.00669228852.533
AACCAAA200.00669228852.570
GCAAAAA200.00669228852.560
GCTTGCA200.00669228852.556
CTTGCAA200.00669228852.557
Produced by FastQC (version 0.11.5)