##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780600_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7667 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.880005217164474 34.0 34.0 34.0 31.0 34.0 2 33.253815051519496 34.0 34.0 34.0 32.0 34.0 3 33.4554584583279 34.0 34.0 34.0 34.0 34.0 4 33.49928263988522 34.0 34.0 34.0 34.0 34.0 5 33.50984739793922 34.0 34.0 34.0 34.0 34.0 6 37.35163688535281 38.0 37.0 38.0 36.0 38.0 7 36.840485196295816 38.0 37.0 38.0 34.0 38.0 8 36.92513368983957 38.0 37.0 38.0 35.0 38.0 9 36.74918481805139 38.0 37.0 38.0 34.0 38.0 10-11 36.45363245076301 38.0 37.0 38.0 34.0 38.0 12-13 36.17438372244685 38.0 37.0 38.0 34.0 38.0 14-15 36.28537889656971 38.0 37.0 38.0 33.5 38.0 16-17 36.03286813616799 38.0 36.0 38.0 32.0 38.0 18-19 35.92715534107212 38.0 36.5 38.0 32.0 38.0 20-21 35.738163558106166 38.0 36.0 38.0 31.0 38.0 22-23 36.136167992695974 38.0 36.5 38.0 32.5 38.0 24-25 34.79033520281727 37.0 34.5 38.0 28.0 38.0 26-27 34.77944437198383 37.0 34.0 38.0 29.0 38.0 28-29 34.899439154819355 37.0 34.5 38.0 28.5 38.0 30-31 34.56371462110343 37.0 34.0 38.0 26.5 38.0 32-33 35.3534628929177 37.0 35.5 38.0 29.5 38.0 34-35 34.79105256293205 37.0 35.0 38.0 28.5 38.0 36-37 34.927416199295685 37.0 35.5 38.0 27.0 38.0 38-39 30.251271683839832 34.0 26.0 37.0 11.0 38.0 40-41 32.709534368070955 36.5 30.5 37.0 18.5 38.0 42-43 34.79320464327638 37.0 34.5 38.0 28.5 38.0 44-45 35.34452849876092 37.0 35.5 38.0 31.0 38.0 46-47 35.27337941828616 38.0 36.0 38.0 30.5 38.0 48-49 34.938763532020346 37.5 35.0 38.0 28.0 38.0 50-51 34.67744880657363 37.0 34.0 38.0 28.0 38.0 52-53 34.37707056214947 37.0 34.0 38.0 26.0 38.0 54-55 34.853919394808926 37.0 35.5 38.0 27.0 38.0 56-57 34.793661145167604 37.0 35.0 38.0 27.0 38.0 58-59 34.24696752315117 37.0 34.0 38.0 24.5 38.0 60-61 34.137146211034306 37.0 34.0 38.0 25.0 38.0 62-63 34.98702230337811 37.0 35.0 38.0 29.0 38.0 64-65 35.12560323464197 37.0 36.0 38.0 29.0 38.0 66-67 33.144841528629186 37.0 32.5 38.0 21.5 38.0 68-69 30.357440980826922 35.0 24.0 37.5 11.0 38.0 70-71 26.830507369244813 29.5 17.0 35.5 10.5 37.5 72-73 21.50691274292422 22.0 11.0 29.5 10.0 36.5 74-75 20.947306638841788 21.0 11.0 28.0 11.0 36.0 76 21.106821442545975 21.0 11.0 29.0 11.0 36.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21486818833643895 1101 2 -0.19503447619362646 1101 3 0.3672867886931144 1101 4 -0.45720264878494277 1101 5 0.11420654819908549 1101 6 -0.541414492743904 1101 7 0.6448615815272589 1101 8 -0.43074125862191437 1101 9 0.9000617515645999 1101 10-11 1.2141221728591987 1101 12-13 0.6390368211233692 1101 14-15 1.1658172070822133 1101 16-17 0.8299419350083284 1101 18-19 0.08589641328814679 1101 20-21 -0.47944178995143005 1101 22-23 0.9868653340289981 1101 24-25 -3.1553293738891313 1101 26-27 -0.6706492693053789 1101 28-29 1.3199655360102085 1101 30-31 2.5400679961116452 1101 32-33 0.7917388545834783 1101 34-35 1.1034053606323724 1101 36-37 0.9886886136749808 1101 38-39 3.884183132542102 1101 40-41 2.395236526560197 1101 42-43 1.7593505958902043 1101 44-45 0.6108429851453323 1101 46-47 -0.20708320051603835 1101 48-49 -1.9690910083380757 1101 50-51 -2.2565619396237118 1101 52-53 -1.1974620627104002 1101 54-55 -0.7222106346014812 1101 56-57 -2.8896633310503574 1101 58-59 -5.2435582697042165 1101 60-61 -3.408789148382475 1101 62-63 -1.2492351128185533 1101 64-65 -0.529298849665345 1101 66-67 -2.4724275919030774 1101 68-69 -1.645116623305622 1101 70-71 0.9332323467710175 1101 72-73 -1.7028216339446622 1101 74-75 1.6837540520297551 1101 76 -1.7061649670525192 1102 1 -1.1594008175115178 1102 2 -1.6687186867199415 1102 3 0.26494760740656886 1102 4 0.4901657722676873 1102 5 -0.8126940365962412 1102 6 0.5229129926362148 1102 7 -1.4808694126247843 1102 8 -0.547700322949396 1102 9 0.7626348509798007 1102 10-11 -0.18792461076652955 1102 12-13 1.0747093357432504 1102 14-15 -1.6061126174791909 1102 16-17 -0.6744440299039454 1102 18-19 -0.9579632358346686 1102 20-21 1.4226049936742982 1102 22-23 0.6140583164851492 1102 24-25 -7.070534052251702 1102 26-27 -2.1881931289545022 1102 28-29 0.5421877582324299 1102 30-31 2.358781446404045 1102 32-33 0.9905692639401948 1102 34-35 1.5434638401645415 1102 36-37 -2.1633581699507474 1102 38-39 0.5786275769865448 1102 40-41 3.1847102107707173 1102 42-43 2.059058198229387 1102 44-45 2.302363452981588 1102 46-47 0.9756653374956556 1102 48-49 -5.15183954634977 1102 50-51 -1.3033455653547037 1102 52-53 -0.3948304837630303 1102 54-55 1.4356841022406215 1102 56-57 -0.4671487111673187 1102 58-59 0.14094465427239555 1102 60-61 -5.666099089902939 1102 62-63 -0.6059602590173867 1102 64-65 1.4663151854223813 1102 66-67 2.511490536751886 1102 68-69 -1.8234791964050387 1102 70-71 -0.44980858890149733 1102 72-73 -1.4250438561668837 1102 74-75 0.5317072684040234 1102 76 -0.8289719845963788 1103 1 0.28557525904350456 1103 2 0.6686018874427404 1103 3 -0.031703110296781745 1103 4 0.5427973512150572 1103 5 0.5030954370879712 1103 6 -0.22828317961258904 1103 7 1.0741545108201933 1103 8 0.7561274282467707 1103 9 0.9707688222716655 1103 10-11 0.09796055669757919 1103 12-13 -0.015003582917039182 1103 14-15 0.06733235859736908 1103 16-17 0.7920631471295465 1103 18-19 1.4116539890457176 1103 20-21 -1.2496438101534508 1103 22-23 0.7217138188774825 1103 24-25 -4.294218262778017 1103 26-27 -1.3676189662750673 1103 28-29 -0.38963042358574995 1103 30-31 1.3683508243944758 1103 32-33 0.774062086906703 1103 34-35 -0.2400289828019666 1103 36-37 -0.26383663885026465 1103 38-39 1.033173031531998 1103 40-41 -0.3547634734398031 1103 42-43 -0.6371140505744464 1103 44-45 0.9542773285796713 1103 46-47 2.56059356716073 1103 48-49 -3.804949594196664 1103 50-51 0.9681855351237587 1103 52-53 1.8252652100168731 1103 54-55 0.6868802744894253 1103 56-57 1.569932628545608 1103 58-59 0.7438154676695277 1103 60-61 -6.343132582725907 1103 62-63 -1.8224674360508786 1103 64-65 -0.9989958193623139 1103 66-67 -0.8234376929131813 1103 68-69 0.011449033260035435 1103 70-71 -0.48090906737039774 1103 72-73 -2.1068620379850636 1103 74-75 0.9766833449590493 1103 76 1.5059562450686954 1104 1 0.5620904105586604 1104 2 0.6799655238063735 1104 3 0.2978423442486715 1104 4 -0.9988693154516071 1104 5 0.3364287704213069 1104 6 -0.23585893718834683 1104 7 1.089306025971709 1104 8 0.9720365191558642 1104 9 1.1222839737868213 1104 10-11 -0.4456000493630228 1104 12-13 0.7709812655678121 1104 14-15 1.624150540415549 1104 16-17 0.7882752683416641 1104 18-19 0.1275630799548111 1104 20-21 -0.007219567729215726 1104 22-23 1.1118653340289981 1104 24-25 1.518281737221983 1104 26-27 2.037684064027964 1104 28-29 2.47968775823243 1104 30-31 2.0331235516672024 1104 32-33 0.9653499656945783 1104 34-35 -0.5771501949231848 1104 36-37 1.2039663914527665 1104 38-39 2.411960910319877 1104 40-41 2.145236526560197 1104 42-43 1.3912950403346471 1104 44-45 1.2913985407008823 1104 46-47 1.890139021706183 1104 48-49 0.49618676943970286 1104 50-51 1.354549171487399 1104 52-53 1.7608712706229284 1104 54-55 1.2152893653985188 1104 56-57 2.4297811133940854 1104 58-59 0.6661639525180121 1104 60-61 1.4939886293953109 1104 62-63 0.8271537760703396 1104 64-65 1.0123678170013264 1104 66-67 0.8470168525413655 1104 68-69 0.820161154472153 1104 70-71 3.0721212356599032 1104 72-73 -0.716710522833548 1104 74-75 -0.9759681701924698 1104 76 1.1271683662808165 1105 1 0.7994092511383712 1105 2 0.6745307411976782 1105 3 -0.05541852531654712 1105 4 0.36888430773679914 1105 5 -0.36646978030333344 1105 6 0.1590685990435361 1105 7 0.9516248665514198 1105 8 -0.7833982634528311 1105 9 0.7672115100187042 1105 10-11 -0.5552014986383824 1105 12-13 -0.6049245315336407 1105 14-15 1.3469766273720651 1105 16-17 1.3118259929793439 1105 18-19 1.6656065582156856 1105 20-21 1.5942297076331045 1105 22-23 0.2513580876521928 1105 24-25 1.4992599980915458 1105 26-27 0.8003652234482459 1105 28-29 0.7161008017106951 1105 30-31 2.2043192038411092 1105 32-33 1.0695166323612497 1105 34-35 -3.6441791804304273 1105 36-37 0.20849537696000908 1105 38-39 2.230801490030025 1105 40-41 0.6343669613428062 1105 42-43 -0.06794409010013425 1105 44-45 1.184514482729874 1105 46-47 1.2868781521409645 1105 48-49 0.12390416074404698 1105 50-51 2.5502013454004455 1105 52-53 2.182972719898295 1105 54-55 2.0386589306159095 1105 56-57 0.46420140324915593 1105 58-59 1.2576494597643872 1105 60-61 1.615365440989514 1105 62-63 -2.1357085427702387 1105 64-65 1.4308460778708891 1105 66-67 1.308973374280491 1105 68-69 0.6833857921533166 1105 70-71 2.0684980472541064 1105 72-73 0.1836517960070303 1105 74-75 1.8383434240104322 1105 76 0.395284308309801 1106 1 0.24661422008247058 1106 2 0.6144893333301837 1106 3 -0.05334813194180299 1106 4 -0.9810121725944683 1106 5 0.12214305613559162 1106 6 0.34747439614498177 1106 7 -0.02974159307591151 1106 8 0.7756079477272948 1106 9 0.6222839737868213 1106 10-11 0.3133285220655537 1106 12-13 1.2501479322344693 1106 14-15 0.45153149279649796 1106 16-17 0.8180371731035692 1106 18-19 0.3954202228119499 1106 20-21 0.8023042417946016 1106 22-23 0.8559129530766185 1106 24-25 -0.448980167539915 1106 26-27 0.5674459687898619 1106 28-29 0.8279020439467217 1106 30-31 0.5003854564291075 1106 32-33 -0.001911939067319679 1106 34-35 0.035945043172056046 1106 36-37 2.242656867643241 1106 38-39 0.5071990055579754 1106 40-41 2.9666650979887663 1106 42-43 1.7633188498584573 1106 44-45 1.4402080645104078 1106 46-47 1.9794247359918984 1106 48-49 1.963448674201608 1106 50-51 1.2116920286302602 1106 52-53 2.814442699194359 1106 54-55 1.4444560320651902 1106 56-57 1.9952573038702823 1106 58-59 1.6572353810894356 1106 60-61 -0.13101137060469625 1106 62-63 1.8539394903560478 1106 64-65 0.315939245572757 1106 66-67 3.6267787573032706 1106 68-69 1.632661154472153 1106 70-71 -1.1302597167210457 1106 72-73 -0.6155200466430735 1106 74-75 0.6758175440932455 1106 76 2.912882651995101 1107 1 0.9537570772253261 1107 2 0.30496552380637354 1107 3 0.27284234424867293 1107 4 0.39279735121505865 1107 5 -0.04690456291202594 1107 6 -0.019192270521685373 1107 7 0.8559726926383746 1107 8 0.5970365191558642 1107 9 -0.9110493595465172 1107 10-11 0.8002332839703143 1107 12-13 0.3918145989011421 1107 14-15 -0.35084945958445246 1107 16-17 1.3716086016749998 1107 18-19 0.5525630799548082 1107 20-21 -0.16555290106254716 1107 22-23 0.4535320006956667 1107 24-25 0.05578173722197732 1107 26-27 -0.7039826026387104 1107 28-29 0.01718775823243135 1107 30-31 1.495623551667208 1107 32-33 1.4945166323612469 1107 34-35 1.6645164717434895 1107 36-37 2.349799724786095 1107 38-39 1.7536275769865455 1107 40-41 0.5202365265601969 1107 42-43 0.8037950403346485 1107 44-45 0.6747318740342152 1107 46-47 0.7901390217061888 1107 48-49 1.6086867694397 1107 50-51 2.529549171487396 1107 52-53 -0.3474620627103988 1107 54-55 -0.19721063460148258 1107 56-57 2.088114446727424 1107 58-59 0.6619972858513421 1107 60-61 2.0773219627286394 1107 62-63 -0.40201289059633183 1107 64-65 1.6873678170013235 1107 66-67 -1.9029831474586345 1107 68-69 0.41599448780549153 1107 70-71 -0.44454543100676247 1107 72-73 1.1249561438331135 1107 74-75 -0.10513483685913272 1107 76 -0.8894983003858528 1108 1 -1.1362429227746738 1108 2 0.644965523806377 1108 3 -0.45715765575133105 1108 4 0.10279735121505951 1108 5 0.22309543708797008 1108 6 0.32080772947831804 1108 7 0.0959726926383766 1108 8 -0.9329634808441369 1108 9 -0.031049359546514665 1108 10-11 0.9752332839703115 1108 12-13 1.6368145989011396 1108 14-15 0.6391505404155495 1108 16-17 0.14660860167499834 1108 18-19 0.37256307995480853 1108 20-21 1.8194470989374523 1108 22-23 -0.17646799930432877 1108 24-25 0.955781737221983 1108 26-27 -0.5439826026387067 1108 28-29 -1.1378122417675698 1108 30-31 -0.34437644833279535 1108 32-33 1.4295166323612492 1108 34-35 0.7445164717434878 1108 36-37 2.059799724786089 1108 38-39 2.7186275769865453 1108 40-41 -0.024763473439804784 1108 42-43 -0.20620495966534946 1108 44-45 1.5297318740342192 1108 46-47 2.505139021706178 1108 48-49 2.2786867694397017 1108 50-51 2.1345491714874 1108 52-53 0.7125379372895964 1108 54-55 0.29778936539852197 1108 56-57 1.7581144467274257 1108 58-59 -1.0580027141486639 1108 60-61 1.3823219627286392 1108 62-63 0.172987109403671 1108 64-65 0.7473678170013258 1108 66-67 1.1420168525413672 1108 68-69 4.4259944878054895 1108 70-71 0.21545456899324122 1108 72-73 0.754956143833116 1108 74-75 -0.5051348368591313 1108 76 -3.43949830038585 1109 1 -1.3762429227746757 1109 2 0.6049655238063707 1109 3 0.30284234424867407 1109 4 0.4227973512150598 1109 5 0.4230954370879729 1109 6 8.077294783177535E-4 1109 7 -0.1440273073616254 1109 8 0.9070365191558665 1109 9 -0.07104935954651381 1109 10-11 -0.18476671602968509 1109 12-13 -0.9231854010988627 1109 14-15 0.6591505404155455 1109 16-17 1.546608601674997 1109 18-19 0.992563079954806 1109 20-21 1.8794470989374545 1109 22-23 0.7035320006956667 1109 24-25 -1.9642182627780187 1109 26-27 -0.16398260263870412 1109 28-29 -2.2578122417675672 1109 30-31 0.8556235516672075 1109 32-33 0.029516632361243467 1109 34-35 -0.6754835282565139 1109 36-37 -0.22020027521390517 1109 38-39 0.7986275769865436 1109 40-41 0.9752365265601952 1109 42-43 0.3537950403346528 1109 44-45 -0.9302681259657817 1109 46-47 0.4451390217061828 1109 48-49 0.6986867694397034 1109 50-51 -0.645450828512601 1109 52-53 -0.8074620627103997 1109 54-55 -2.3222106346014755 1109 56-57 -2.121885553272577 1109 58-59 -1.4580027141486624 1109 60-61 -1.3176780372713566 1109 62-63 -2.507012890596336 1109 64-65 -1.0526321829986713 1109 66-67 -2.097983147458635 1109 68-69 -1.6140055121945096 1109 70-71 1.0754545689932407 1109 72-73 -0.24504385616688396 1109 74-75 -2.0851348368591367 1109 76 -2.59949830038585 1110 1 -3.123515650047402 1110 2 -0.4223072034663531 1110 3 0.013751435157765002 1110 4 -0.3662935578758493 1110 5 0.4576408916334245 1110 6 -1.2737377250671358 1110 7 -0.19857276190708006 1110 8 0.4379456100649577 1110 9 -0.6201402686374209 1110 10-11 -0.3111303523933273 1110 12-13 1.189541871628414 1110 14-15 1.2718778131428223 1110 16-17 0.5193358744022731 1110 18-19 -0.6110732836815487 1110 20-21 1.3185380080283622 1110 22-23 -0.5282861811225175 1110 24-25 -2.2714909900507436 1110 26-27 1.5641992155431126 1110 28-29 0.3376423036869767 1110 30-31 -0.5634673574237041 1110 32-33 0.06951663236124972 1110 34-35 2.0326982899252997 1110 36-37 2.031617906604275 1110 38-39 1.3286275769865448 1110 40-41 -1.536581655257983 1110 42-43 0.2947041312437406 1110 44-45 1.9088227831251245 1110 46-47 1.8333208398880032 1110 48-49 2.5814140421669762 1110 50-51 0.2181855351237587 1110 52-53 1.8252652100168731 1110 54-55 0.9141530017621591 1110 56-57 0.7972053558183276 1110 58-59 -2.119820895966839 1110 60-61 -2.9113144009077274 1110 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1.6424650760407857 2116 70-71 3.7142780984050034 2116 72-73 -0.042690914990412665 2116 74-75 2.294865163140866 2116 76 -0.3336159474446738 2117 1 0.6662570772253247 2117 2 0.8049655238063735 2117 3 0.4228423442486715 2117 4 0.5427973512150572 2117 5 -0.12190456291202878 2117 6 -0.006692270521682531 2117 7 0.9434726926383732 2117 8 -0.2154634808441358 2117 9 -0.21104935954651438 2117 10-11 1.106483283970313 2117 12-13 -0.6456854010988593 2117 14-15 1.092900540415549 2117 16-17 -0.6596413983250002 2117 18-19 0.5650630799548111 2117 20-21 0.3469470989374557 2117 22-23 0.3097820006956695 2117 24-25 -2.544218262778017 2117 26-27 -2.2852326026387075 2117 28-29 -4.58281224176757 2117 30-31 -1.0606264483327976 2117 32-33 -2.1492333676387503 2117 34-35 0.49576647174348665 2117 36-37 -0.3064502752139049 2117 38-39 -2.2026224230134552 2117 40-41 -3.073513473439803 2117 42-43 -1.0774549596653529 2117 44-45 -0.750268125965782 2117 46-47 -1.703610978293817 2117 48-49 -0.9725632305602971 2117 50-51 0.22954917148739895 2117 52-53 2.3025379372895998 2117 54-55 0.6527893653985188 2117 56-57 0.7318644467274211 2117 58-59 -2.5942527141486593 2117 60-61 -1.7976780372713606 2117 62-63 -0.33951289059633183 2117 64-65 -5.206382182998674 2117 66-67 -4.0592331474586345 2117 68-69 -0.6902555121945113 2117 70-71 -4.782045431006761 2117 72-73 -1.3937938561668837 2117 74-75 -2.580134836859134 2117 76 -1.6019983003858513 2118 1 0.16258060663709273 2118 2 0.6873184649828445 2118 3 -1.3418635381042705 2118 4 0.3663267629797602 2118 5 -0.08513985702967375 2118 6 -0.4956628587569796 2118 7 0.7853844573442572 2118 8 0.40586004856763225 2118 9 -2.8286964183700434 2118 10-11 -1.0718255395591 2118 12-13 -0.7890677540400333 2118 14-15 -0.706731812525625 2118 16-17 -7.282803163030884 2118 18-19 -1.9533192729863629 2118 20-21 -1.1934940775331313 2118 22-23 -0.18470329342197545 2118 24-25 0.48519350192786703 2118 26-27 -1.6804531908739975 2118 28-29 -2.751929888826396 2118 30-31 -1.0514352718622106 2118 32-33 -1.0481304264622793 2118 34-35 0.11157529527289256 2118 36-37 -5.929612039919789 2118 38-39 -2.274313599484042 2118 40-41 -0.6635870028515711 2118 42-43 -0.13444025378300495 2118 44-45 -2.98556224361284 2118 46-47 -0.8819198018232228 2118 48-49 -0.17660734820735513 2118 50-51 -1.292509652042014 2118 52-53 -4.682756180357458 2118 54-55 -1.3398576934250102 2118 56-57 -0.3912973179784629 2118 58-59 -2.4380027141486593 2118 60-61 -2.6800309784478316 2118 62-63 -4.76230700824339 2118 64-65 -1.4655733594692677 2118 66-67 -0.866218441576283 2118 68-69 -4.269299629841569 2118 70-71 -1.344545431006761 2118 72-73 -0.48386738557864817 2118 74-75 -2.734546601565018 2118 76 0.01932522902591316 2119 1 0.5704237438919932 2119 2 -0.9950344761936236 2119 3 0.1561756775820058 2119 4 0.5427973512150572 2119 5 0.03642877042130266 2119 6 -0.05252560385501681 2119 7 -2.2106939740282954 2119 8 -1.0529634808441344 2119 9 -1.3110493595465158 2119 10-11 0.20856661730364578 2119 12-13 -0.9498520677655264 2119 14-15 -0.9675161262511196 2119 16-17 -0.8867247316583331 2119 18-19 -0.44743692004519175 2119 20-21 -0.9072195677292072 2119 22-23 -0.8964679993043276 2119 24-25 0.955781737221983 2119 26-27 -0.8706492693053747 2119 28-29 -0.12447890843423437 2119 30-31 -1.3377097816661347 2119 32-33 -1.330483367638756 2119 34-35 0.631183138410158 2119 36-37 0.5331330581194322 2119 38-39 -3.4880390896801217 2119 40-41 -1.304763473439806 2119 42-43 -1.7462049596653557 2119 44-45 -1.3836014592991148 2119 46-47 -1.668194311627154 2119 48-49 -0.5413132305602915 2119 50-51 1.5878825048207332 2119 52-53 -0.38079539604373025 2119 54-55 -1.855543967934814 2119 56-57 -7.095218886605913 2119 58-59 -2.23800271414866 2119 60-61 -1.6976780372713591 2119 62-63 -3.960346223929662 2119 64-65 -0.6792988496653365 2119 66-67 -0.31131648079196594 2119 68-69 -3.7006721788611756 2119 70-71 -0.8112120976734296 2119 72-73 3.208289477166449 2119 74-75 0.9615318298075302 2119 76 2.160501699614148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 4.0 24 24.0 25 40.0 26 56.0 27 109.0 28 154.0 29 278.0 30 425.0 31 574.0 32 793.0 33 1126.0 34 1566.0 35 1611.0 36 606.0 37 299.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 4.304160688665711 15.808008347463154 13.57767053606365 66.31016042780749 2 2.425981479066128 3.8346158862658144 79.61392982913786 14.125472805530196 3 2.556410590843876 17.007956175818443 71.16212338593975 9.27350984739794 4 2.556410590843876 58.04095474109822 22.759880005217166 16.642754662840744 5 2.2042519890439545 29.816094952393374 63.23203338985262 4.7476196687100565 6 3.978087909221338 65.51454284596322 12.260336507108386 18.247032737707055 7 4.27807486631016 28.746576235815834 8.438763532020346 58.536585365853654 8 2.6346680579105257 65.43628537889657 15.625407590974305 16.303638972218597 9 2.9868266597104474 15.455849745663233 3.912873353332464 77.64445024129387 10-11 3.430285639754793 37.58314855875832 38.835268031824704 20.15129776966219 12-13 9.749576105386721 6.1432111647319685 39.689578713968956 44.41763401591235 14-15 11.327768357897483 10.075648884831095 10.688665710186514 67.90791704708491 16-17 37.250554323725055 32.75727142298161 2.8498760923438113 27.142298160949522 18-19 37.680970392591625 17.184035476718403 3.1824703273770703 41.9525238033129 20-21 13.14073301160819 6.3910264771096905 2.967262292943785 77.50097821833833 22-23 11.784270249119604 42.12208164862397 3.091169949132646 43.00247815312378 24-25 37.230989956958396 40.90256945350202 4.088952654232425 17.77748793530716 26-27 44.580670405634535 39.676535802791186 9.227859658275728 6.514934133298553 28-29 41.065605843224205 17.992695969740446 6.508412677709664 34.433285509325685 30-31 39.34394156775792 12.012521194730665 41.29385678883527 7.349680448676145 32-33 17.894874135907134 37.51793400286944 34.961523412025564 9.62566844919786 34-35 31.459501760793007 44.61327768357897 17.470979522629452 6.456241032998565 36-37 10.982131211686449 34.518064431981216 12.416851441241686 42.08295291509065 38-39 9.253945480631277 13.421155601930352 35.033259423503324 42.291639493935044 40-41 6.162775531498631 10.336507108386591 44.280683448545716 39.22003391156906 42-43 40.09390896047998 5.14542845963219 42.291639493935044 12.469023085952784 44-45 8.445284987609234 33.50071736011478 40.57649667405765 17.477500978218337 46-47 40.824311986435376 6.084518064431981 18.025303247684885 35.06586670144776 48-49 34.074605451936876 3.5215860179992173 12.795095865397158 49.608712664666754 50-51 10.486500586931003 3.678100952132516 44.502412938567886 41.332985522368595 52-53 8.673535933220295 3.7759227859658275 73.19029607408373 14.360245206730143 54-55 5.367157949654363 3.7107082300769534 49.308725707577935 41.613408112690756 56-57 39.604799791313425 4.447632711621234 20.640406938828747 35.3071605582366 58-59 7.577931394287204 17.379679144385026 63.08856136689709 11.953828094430676 60-61 33.585496282770315 47.62619016564497 14.39285248467458 4.395461066910134 62-63 5.034563714621103 86.80057388809182 3.2215990609103953 4.943263336376679 64-65 2.315116734055041 89.80044345898004 2.9346550149993478 4.949784791965567 66-67 2.4977174905438897 87.77227077083604 3.032476848832659 6.6975348897874 68-69 2.6933611582105126 82.58119212208165 4.062866831876875 10.662579887830963 70-71 3.482457284465893 75.35541932959437 6.54754141124299 14.614581974696753 72-73 5.262814660232164 60.98213121168645 9.834355028042259 23.920699100039126 74-75 5.497587061432112 57.610538672231634 10.629972609886526 26.26190165644972 76 4.591104734576758 59.20177383592018 9.32568149210904 26.881439937394024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 2.5 24 2.0 25 0.0 26 5.0 27 9.0 28 8.0 29 8.0 30 11.0 31 11.5 32 8.5 33 8.0 34 10.5 35 14.5 36 18.0 37 20.0 38 37.5 39 124.0 40 663.0 41 1501.5 42 1870.0 43 1754.0 44 1364.0 45 826.5 46 563.0 47 430.0 48 229.5 49 137.0 50 112.0 51 92.5 52 58.5 53 33.5 54 23.0 55 26.5 56 25.5 57 29.0 58 37.0 59 39.0 60 43.0 61 41.5 62 38.0 63 31.0 64 21.5 65 20.5 66 20.0 67 18.0 68 11.0 69 3.5 70 2.0 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 7667.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.199165253684622 #Duplication Level Percentage of deduplicated Percentage of total 1 73.02989130434783 14.021129516107994 2 11.616847826086957 4.460675622799009 3 7.065217391304348 4.069388287465762 4 2.1059782608695654 1.6173209860440851 5 1.2228260869565217 1.173862005999739 6 0.6114130434782609 0.7043172035998435 7 0.4755434782608696 0.639102647710969 8 0.5434782608695652 0.8347463153775923 9 0.33967391304347827 0.5869310029998696 >10 2.309782608695652 7.799660884309379 >50 0.2717391304347826 4.186774488065737 >100 0.2717391304347826 12.364679796530584 >500 0.06793478260869565 8.895265423242469 >1k 0.06793478260869565 38.64614581974697 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 2963 38.64614581974697 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT 682 8.895265423242469 RNA PCR Primer, Index 24 (95% over 23bp) TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 456 5.947567497065346 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 264 3.443328550932568 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT 116 1.512977696621886 No Hit CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 112 1.4608060519107866 RNA PCR Primer, Index 24 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT 94 1.2260336507108387 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT 94 1.2260336507108387 No Hit TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT 77 1.0043041606886656 RNA PCR Primer, Index 24 (95% over 22bp) CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 56 0.7304030259553933 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 39 0.5086735359332203 No Hit ACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCT 31 0.4043302465110213 RNA PCR Primer, Index 24 (96% over 25bp) TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG 31 0.4043302465110213 No Hit CTTATACACATCTCCGAGCCCACGAGCCGTGATAGCATCTCGTATGCCGT 30 0.3912873353332464 No Hit CTTATACACATCTCCGAGCCCACGCGACGTGATAGCATCTCGTATGCCGT 29 0.3782444241554715 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCG 28 0.36520151297769665 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGTCTT 26 0.33911569062214686 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTAGTATGCCGT 22 0.2869440459110474 No Hit ATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTT 21 0.2739011347332725 RNA PCR Primer, Index 34 (96% over 28bp) TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 17 0.22172949002217296 No Hit CTTATACACATCTCCGAGCCCACGAGACGCGATAGCATCTCGTATGCCGT 17 0.22172949002217296 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCC 17 0.22172949002217296 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCACGTATGCCGT 16 0.20868657884439806 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT 16 0.20868657884439806 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATCGCATCTCGTATGCCGT 16 0.20868657884439806 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCG 16 0.20868657884439806 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCCCGTATGCCGT 16 0.20868657884439806 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGTCTT 15 0.1956436676666232 No Hit CTTATACACATCTCCGAGCACACGAGACGTGATAGCATCTCGTATGCCGT 15 0.1956436676666232 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT 14 0.18260075648884833 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCC 14 0.18260075648884833 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 13 0.16955784531107343 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATTTCGTATGCCGT 13 0.16955784531107343 No Hit CTTATACACATATCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 13 0.16955784531107343 No Hit ATACACATCTCCGAGCCCACGAGCCGTGATAGCATCTCGTATGCCGTCTT 12 0.15651493413329856 RNA PCR Primer, Index 24 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACGTGATAGCCTCTCGTATGCCGT 12 0.15651493413329856 No Hit CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTA 12 0.15651493413329856 No Hit CTTATACACATCTCCGCGCCCACGAGACGTGATAGCATCTCGTATGCCGT 11 0.1434720229555237 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCC 11 0.1434720229555237 No Hit CTTATACACCTCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 11 0.1434720229555237 No Hit CTTAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 11 0.1434720229555237 RNA PCR Primer, Index 24 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGTCTT 11 0.1434720229555237 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAAATCGTATGCCGT 11 0.1434720229555237 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 11 0.1434720229555237 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGC 9 0.11738620059997393 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT 9 0.11738620059997393 No Hit CTTATACACATCTCCGAGCCCAAGAGACGTGATAGCATCTCGTATGCCGT 9 0.11738620059997393 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT 9 0.11738620059997393 No Hit CTTATACACATCTCCGAGCCCACGTGACGTGATAGCATCTCGTATGCCGT 9 0.11738620059997393 No Hit CTTATACACATCTCCGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC 8 0.10434328942219903 No Hit CTTATCACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC 8 0.10434328942219903 No Hit CTTATACACATCTCCGAGCCAACGAGACGTGATAGCATCTCGTATGCCGT 8 0.10434328942219903 No Hit CTTATACACATCTACGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 8 0.10434328942219903 No Hit GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAG 8 0.10434328942219903 No Hit CTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTGCTTGA 8 0.10434328942219903 RNA PCR Primer, Index 24 (96% over 30bp) CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTCTGCCGT 8 0.10434328942219903 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 8 0.10434328942219903 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.15651493413329856 0.0 2 0.0 0.0 0.0 0.15651493413329856 0.0 3 0.0 0.0 0.0 0.15651493413329856 0.0 4 0.0 0.0 0.0 0.15651493413329856 0.0 5 0.0 0.0 0.0 0.1826007564888483 0.0 6 0.0 0.0 0.0 0.1826007564888483 0.0 7 0.0 0.0 0.0 0.1826007564888483 0.0 8 0.0 0.0 0.0 0.1826007564888483 0.0 9 0.0 0.0 0.0 0.1826007564888483 0.0 10 0.0 0.0 0.0 0.1956436676666232 0.0 11 0.0 0.0 0.0 0.22172949002217296 0.0 12 0.0 0.0 0.0 0.2608582235554976 0.0 13 0.0 0.0 0.0 0.2999869570888222 0.0 14 0.0 0.0 0.0 0.3130298682665971 0.0 15 0.0 0.0 0.0 0.3782444241554715 0.0 16 0.0 0.0 0.0 0.4043302465110213 0.0 17 0.0 0.0 0.0 0.5347593582887701 0.0 18 0.0 0.0 0.0 0.6912742924220686 0.0 19 0.0 0.0 0.0 0.9651754271553411 0.0 20 0.0 0.0 0.0 1.09560453893309 0.0 21 0.0 0.0 0.0 1.1216903612886395 0.0 22 0.0 0.0 0.0 1.173862005999739 0.0 23 0.0 0.0 0.0 1.2521194730663885 0.0 24 0.0 0.0 0.0 1.2651623842441633 0.0 25 0.0 0.0 0.0 1.2651623842441633 0.0 26 0.0 0.0 0.0 1.2651623842441633 0.0 27 0.0 0.0 0.0 1.2651623842441633 0.0 28 0.0 0.0 0.0 1.2651623842441633 0.0 29 0.0 0.0 0.0 1.2651623842441633 0.0 30 0.0 0.0 0.0 1.2782052954219383 0.0 31 0.0 0.0 0.0 1.3695056736663622 0.0 32 0.0 0.0 0.0 1.408634407199687 0.0 33 0.0 0.0 0.0 1.526020607799661 0.0 34 0.0 0.0 0.0 1.6173209860440851 0.0 35 0.0 0.0 0.0 1.7738359201773837 0.0 36 0.0 0.0 0.0 1.943393765488457 0.0 37 0.0 0.0 0.0 2.2303378113995045 0.0 38 0.0 0.0 0.0 2.5172818573105515 0.0 39 0.0 0.0 0.0 2.7390113473327244 0.0 40 0.0 0.0 0.0 2.947697926177123 0.0 41 0.0 0.0 0.0 3.1824703273770707 0.0 42 0.0 0.0 0.0 3.430285639754793 0.0 43 0.0 0.0 0.0 3.6128863962436415 0.0 44 0.0 0.0 0.0 3.9389591756880136 0.0 45 0.0 0.0 0.0 4.108517020999087 0.0 46 0.0 0.0 0.0 4.1476457545324115 0.0 47 0.0 0.0 0.0 4.35633233337681 0.0 48 0.0 0.0 0.0 4.538933089865658 0.0 49 0.0 0.0 0.0 4.708490935176731 0.0 50 0.0 0.0 0.0 4.878048780487805 0.0 51 0.0 0.0 0.0 5.008477892265554 0.0 52 0.0 0.0 0.0 5.269336115821051 0.0 53 0.0 0.0 0.0 5.5432372505543235 0.0 54 0.0 0.0 0.0 5.686709273509847 0.0 55 0.0 0.0 0.0 5.738880918220947 0.0 56 0.0 0.0 0.0 5.947567497065345 0.0 57 0.0 0.0 0.0 6.064953697665319 0.0 58 0.0 0.0 0.0 6.247554454154167 0.0 59 0.0 0.0 0.0 6.404069388287466 0.0 60 0.0 0.0 0.0 6.456241032998565 0.0 61 0.0 0.0 0.0 6.482326855354115 0.0 62 0.0 0.0 0.0 6.782313812442937 0.0 63 0.0 0.0 0.0 6.847528368331812 0.0 64 0.0 0.0 0.0 7.03012912482066 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTCGTA 25 2.580391E-6 70.0 38 TAGCACC 20 7.4367985E-5 70.0 33 CACCTCG 25 2.580391E-6 70.0 36 ATAGCAC 20 7.4367985E-5 70.0 32 ACCTCGT 25 2.580391E-6 70.0 37 AGCACCT 20 7.4367985E-5 70.0 34 GCACCTC 20 7.4367985E-5 70.0 35 CTTATAC 565 0.0 55.132744 1 TTATACA 560 0.0 54.375 2 TATACAC 575 0.0 52.95652 3 TTGCAAA 20 0.006692288 52.5 58 AACAACC 20 0.006692288 52.5 66 TGCAAAA 20 0.006692288 52.5 59 TATCGTA 20 0.006692288 52.5 38 GAAAAAC 20 0.006692288 52.5 60 TAGCATA 20 0.006692288 52.5 33 AACCAAA 20 0.006692288 52.5 70 GCAAAAA 20 0.006692288 52.5 60 GCTTGCA 20 0.006692288 52.5 56 CTTGCAA 20 0.006692288 52.5 57 >>END_MODULE