FastQCFastQC Report
Wed 25 May 2016
SRR1780599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75435
Sequences flagged as poor quality0
Sequence length50
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG990.13123881487373235No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATC950.1259362364949957No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG860.1140054351428382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTGA207.8515825E-443.9522556
ACGCGGC301.277703E-436.72429314
TCGGGCC250.002336085535.208512
CTCGTTG250.002351364435.16180441
ATATCGG250.002351364435.16180442
CAGATGT250.002351364435.1618045
ACATCTT250.002351364435.1618045
TTTACAC702.7284841E-1134.5339132
CTTTACA701.0077201E-931.4152981
GCACGAC300.0057045729.34041835
CTATACA454.0313196E-529.3209441
ATGGATC300.00574173729.3015067
ACATTTG300.00574173729.3015065
GATATCG300.00574173729.30150641
CATCGGA300.00574173729.3015069
TATCGGG300.00574173729.30150643
TTCTCGG300.00574173729.30150642
TCTCGGG1350.027.67364343
CCCATCT407.0151314E-427.470167
TACACGT407.0151314E-427.470162
Produced by FastQC (version 0.11.5)