##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780599_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 75435 Sequences flagged as poor quality 0 Sequence length 50 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.54627162457745 16.0 10.0 28.0 7.0 31.0 2 20.310572015642606 22.0 10.0 30.0 7.0 31.0 3 20.242367601246105 22.0 10.0 30.0 7.0 31.0 4 22.118459600980977 22.0 10.0 33.0 7.0 37.0 5 22.229986080731756 23.0 10.0 33.0 7.0 37.0 6 22.05124942003049 22.0 10.0 33.0 8.0 37.0 7 21.936236494995693 22.0 10.0 33.0 8.0 37.0 8 21.8065089149599 21.0 10.0 33.0 8.0 37.0 9 22.33011201696825 22.0 10.0 34.0 8.0 39.0 10 22.17281102936303 21.0 10.0 34.0 8.0 39.0 11 22.558096374362034 23.0 10.0 34.0 8.0 39.0 12 22.169801816133095 21.0 10.0 34.0 8.0 39.0 13 22.28789023662756 22.0 10.0 34.0 8.0 39.0 14 22.61393252469013 22.0 10.0 34.0 8.0 40.0 15 22.334287797441505 20.0 10.0 34.0 8.0 40.0 16 22.257996951017432 20.0 10.0 34.0 7.0 40.0 17 22.357592629416054 21.0 10.0 34.0 7.0 40.0 18 22.055624047192946 19.0 10.0 33.0 7.0 40.0 19 22.134009412076622 19.0 10.0 34.0 7.0 40.0 20 22.200941207662225 20.0 10.0 34.0 7.0 40.0 21 21.92183999469742 19.0 9.0 33.0 7.0 40.0 22 21.93582554517134 19.0 9.0 33.0 7.0 40.0 23 21.855875919665937 19.0 9.0 33.0 7.0 40.0 24 21.42846158944787 18.0 8.0 32.0 7.0 40.0 25 21.39711009478359 18.0 8.0 32.0 7.0 39.0 26 21.138582885928283 18.0 8.0 32.0 7.0 39.0 27 20.827096175515344 17.0 8.0 32.0 7.0 39.0 28 20.71853913965666 17.0 8.0 32.0 7.0 39.0 29 20.473719095910386 17.0 8.0 31.0 7.0 39.0 30 20.31941406508915 17.0 8.0 31.0 7.0 39.0 31 20.267475309869425 16.0 8.0 31.0 7.0 39.0 32 20.00751640485186 16.0 8.0 31.0 7.0 39.0 33 19.96831709418705 16.0 8.0 30.0 7.0 38.0 34 19.65016239146285 16.0 8.0 30.0 7.0 38.0 35 19.47979054815404 15.0 8.0 30.0 7.0 38.0 36 19.408245509378936 15.0 8.0 30.0 7.0 38.0 37 19.224829323258433 15.0 8.0 30.0 7.0 38.0 38 19.252522038841388 15.0 8.0 30.0 7.0 38.0 39 18.965387419632798 15.0 8.0 29.0 7.0 38.0 40 18.418996487041824 14.0 8.0 28.0 7.0 38.0 41 18.563491747862397 14.0 8.0 28.0 7.0 37.0 42 18.556850268443032 14.0 8.0 28.0 7.0 37.0 43 18.58905017564791 14.0 8.0 28.0 7.0 37.0 44 18.54534367336117 14.0 8.0 28.0 7.0 37.0 45 18.61287200901438 14.0 8.0 28.0 7.0 37.0 46 18.417127328163318 14.0 8.0 28.0 7.0 37.0 47 18.307006031682906 13.0 8.0 28.0 7.0 36.0 48 18.242221780340692 13.0 8.0 28.0 7.0 36.0 49 18.009226486379003 13.0 8.0 28.0 7.0 36.0 50 18.022085238947437 13.0 8.0 28.0 7.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -1.9065886654010669 1101 2 -2.0884865621472173 1101 3 -1.018732054916299 1101 4 -0.33074209203034854 1101 5 -1.2601402637206824 1101 6 -2.0487510265868814 1101 7 -2.768851771711901 1101 8 -1.4643731296560638 1101 9 -1.076633251341633 1101 10 -1.6312908608404264 1101 11 -2.1440146017468926 1101 12 -2.0307054304627314 1101 13 -1.735053733181239 1101 14 -2.2600494342675113 1101 15 -2.214691830075438 1101 16 -0.4442704213397235 1101 17 -1.791536527928038 1101 18 -2.260837664916167 1101 19 -2.2990882920211924 1101 20 -2.270615083929126 1101 21 -2.4274559103217364 1101 22 -2.17669266155724 1101 23 -3.112924013474686 1101 24 -2.6242475331684822 1101 25 -3.1322319778933974 1101 26 -3.1082317591492306 1101 27 -2.9719258135763127 1101 28 -2.6321923427756673 1101 29 -0.30184182354026134 1101 30 -2.4170098774004565 1101 31 -2.618764857878819 1101 32 -2.0732793940033076 1101 33 -2.117005411389453 1101 34 -1.8215852995936608 1101 35 -2.343269749466643 1101 36 -1.8149717765063045 1101 37 -1.8967038305208632 1101 38 -1.911027317597906 1101 39 -1.759832031098945 1101 40 -1.038395955896906 1101 41 -1.4122307540204915 1101 42 -1.4019921133332254 1101 43 -1.0208490289770253 1101 44 -0.9196679511985906 1101 45 -1.2915157313131616 1101 46 -1.6996799838060639 1101 47 -1.928519293848943 1101 48 -1.8055482143377866 1101 49 -2.3542662590281225 1101 50 -2.9151854079497976 1102 1 -2.150095158907561 1102 2 -0.9844281205887739 1102 3 -0.1510372497214938 1102 4 -2.39876157254983 1102 5 -0.5896857182661357 1102 6 -1.7119003772362333 1102 7 -2.040442680802812 1102 8 -1.9504120906950249 1102 9 -2.46510727731566 1102 10 -2.358401250450818 1102 11 -2.290930186162477 1102 12 -1.8454781577354602 1102 13 -0.9699563305838375 1102 14 -2.441705278423356 1102 15 -1.8580359859195923 1102 16 -0.9080691226384232 1102 17 -1.8628027616942724 1102 18 -0.9113246779031776 1102 19 -1.238374006306909 1102 20 -1.1183423566563988 1102 21 -1.2445013648671903 1102 22 -1.382212142076721 1102 23 -0.9106512862019578 1102 24 -2.4016825981035446 1102 25 -2.609017692179112 1102 26 -2.3960564344739055 1102 27 -2.117055683706184 1102 28 -2.35491961550294 1102 29 -2.2675885767870163 1102 30 -2.026100786491366 1102 31 -1.3155181046320656 1102 32 -2.427013160237074 1102 33 -1.6923300867141293 1102 34 -1.423533351541714 1102 35 -1.0231398793367745 1102 36 -1.4415951531296791 1102 37 -1.4101778564948901 1102 38 -2.420605239675826 1102 39 -2.1651891739560867 1102 40 -2.6028440078449577 1102 41 -2.098269715059452 1102 42 -1.2622193860604973 1102 43 -1.4370827952107916 1102 44 -2.2797328862635275 1102 45 -2.220736510533939 1102 46 -0.959907256533338 1102 47 -1.322350462680113 1102 48 -1.2263274351170068 1102 49 -1.1346234018852641 1102 50 -1.5033347585991486 1103 1 -1.0093067437828616 1103 2 0.4405115415190295 1103 3 -0.3746897034624439 1103 4 -0.07197154841467324 1103 5 -1.5422199097383817 1103 6 -0.8454482452973764 1103 7 -1.2649800902959711 1103 8 -1.1924072636636502 1103 9 -1.6795883714427724 1103 10 -0.626945095985814 1103 11 -1.3601176358809006 1103 12 -1.5633381738255636 1103 13 -1.3331099910826296 1103 14 -1.1583585366695353 1103 15 -0.2068694533371307 1103 16 -0.4972887525660177 1103 17 -1.0621591575108447 1103 18 -0.7451138975331055 1103 19 -0.5808518824131035 1103 20 -0.4288009246370912 1103 21 -0.9014603351004986 1103 22 0.35696726258940714 1103 23 0.22667775643681765 1103 24 -1.7104517051027415 1103 25 0.2812477945465517 1103 26 -0.82654718266377 1103 27 -0.34376525731840957 1103 28 -0.33351977640398545 1103 29 -0.29638986399538325 1103 30 -1.2577178420022257 1103 31 -1.2973362864502462 1103 32 -0.6488957024735988 1103 33 -0.7440597729570904 1103 34 -0.652495539795936 1103 35 -0.8486426951050774 1103 36 -0.005553318857757006 1103 37 -0.03173859663970191 1103 38 -0.5966309838431627 1103 39 -0.1864281120091853 1103 40 0.1434150428388712 1103 41 0.07212449250289765 1103 42 -0.34170450271375685 1103 43 -0.3890538330225368 1103 44 -2.3506097969634467 1103 45 -0.10030207771012556 1103 46 -0.32523308115119676 1103 47 -0.20585810547979122 1103 48 -1.1361423989142736 1103 49 -1.3505526054250865 1103 50 -0.9696259894921475 1104 1 1.0035098567664207 1104 2 0.688299152138498 1104 3 0.06839848695562267 1104 4 0.1775707158514237 1104 5 1.6062390466241467 1104 6 0.24342877636878413 1104 7 1.6169240903570632 1104 8 0.8712180033488615 1104 9 0.33254162550565525 1104 10 0.506332291868933 1104 11 0.7639287480068013 1104 12 0.15248422470968137 1104 13 -0.36713501396941695 1104 14 0.1534357874073642 1104 15 -1.0893839483020358 1104 16 -0.6163763326697698 1104 17 -0.5270660845782835 1104 18 -0.16508643338907092 1104 19 -0.6814774545827689 1104 20 -0.7232012908256777 1104 21 0.03590615864377966 1104 22 -0.5282936467789163 1104 23 -0.5568895307160666 1104 24 -0.01187068587784168 1104 25 1.6340857560967486 1104 26 0.127297797501015 1104 27 0.010217043566544959 1104 28 -0.06322682553428649 1104 29 -0.2288738432446955 1104 30 -0.31356160153838886 1104 31 -0.668025941622659 1104 32 -0.8248188028703041 1104 33 -0.9569068892219654 1104 34 -0.9816838217611483 1104 35 -1.4245505376439844 1104 36 -0.7472377863585216 1104 37 -0.7677013674666782 1104 38 -1.177345051588052 1104 39 -1.2841546911974682 1104 40 0.04072965986664201 1104 41 -0.4042258279121178 1104 42 -0.9343196995401222 1104 43 -0.740060851637125 1104 44 -0.9178206605581991 1104 45 -0.7705797707220263 1104 46 -0.1975248113922703 1104 47 -0.47470155985879714 1104 48 0.2855847905883202 1104 49 -0.9863475398163004 1104 50 -0.8342125015458564 1105 1 0.6828496740372714 1105 2 0.12419658803593236 1105 3 -0.04536514404938785 1105 4 -0.7648843386725979 1105 5 -0.2547983833788017 1105 6 0.2577965924607355 1105 7 0.1771952368351073 1105 8 -0.629666169949104 1105 9 -0.20201481422319745 1105 10 -0.5932108852604507 1105 11 -0.3585292537615459 1105 12 -0.4062396184969188 1105 13 -0.2983168589443643 1105 14 0.1882869515688732 1105 15 -0.4162333441169501 1105 16 0.09457268000338104 1105 17 -0.9445432434347545 1105 18 1.3065463399678379 1105 19 -1.0699979379308395 1105 20 -1.0686920070060495 1105 21 0.42884750848168096 1105 22 -0.42012977999436174 1105 23 -0.7806590562097284 1105 24 0.4295528331318863 1105 25 -0.19534247850389974 1105 26 0.0810997426822695 1105 27 0.10423413758363864 1105 28 -0.18133764192096535 1105 29 0.6902979810995404 1105 30 -0.04948850987909026 1105 31 -1.425541414655374 1105 32 -0.04954618277009715 1105 33 0.2395269451429023 1105 34 0.4564977284893672 1105 35 -0.07434423054112571 1105 36 -0.6327373442718702 1105 37 -0.18982043613747024 1105 38 0.33370711463652825 1105 39 -0.20450541327232585 1105 40 0.010520405519457654 1105 41 -0.1330793498690852 1105 42 -0.06812459196570231 1105 43 -0.20064145876945716 1105 44 0.4351855286548876 1105 45 0.3070801172826876 1105 46 0.578631982005902 1105 47 0.048434308104656765 1105 48 0.008249099459526121 1105 49 0.492727025465161 1105 50 0.18943913417474434 1106 1 -1.7637315225439245 1106 2 -0.42003418119483626 1106 3 -0.8836130072972495 1106 4 -1.3480039967922544 1106 5 -1.9601402637206817 1106 6 -1.0088700742059302 1106 7 -1.214685105045234 1106 8 -2.765563605846541 1106 9 -1.835561822770206 1106 10 -2.281886098935665 1106 11 -2.496990792223084 1106 12 -2.143205430462732 1106 13 -2.21541087603838 1106 14 -1.0106446723627514 1106 15 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1107 1 -2.0840440225439245 1107 2 -2.124200847861502 1107 3 -2.1716338406305837 1107 4 -2.0818581634589215 1107 5 -2.4867027637206824 1107 6 -2.0520992408725967 1107 7 -2.753226771711901 1107 8 -2.380667772513206 1107 9 -2.038686822770206 1107 10 -2.1558444322689994 1107 11 -2.1808449588897503 1107 12 -2.4057054304627314 1107 13 -2.3779108760383814 1107 14 -2.511165505696084 1107 15 -2.479200758646865 1107 16 -1.9040918499111505 1107 17 -2.0437686707851803 1107 18 -1.7184269506304517 1107 19 -2.2789990063069077 1107 20 -2.833115083929126 1107 21 -3.2243309103217364 1107 22 -2.935621232985813 1107 23 -2.269174013474686 1107 24 -2.6309439617399093 1107 25 -2.6199551921791127 1107 26 -1.7856871162920882 1107 27 -2.747595456433455 1107 28 -2.4212548427756673 1107 29 -1.7638953949688343 1107 30 -1.4482598774004565 1107 31 -1.0629612864502462 1107 32 -1.3991722511461653 1107 33 -2.5913357685323106 1107 34 -1.9175674424508031 1107 35 -2.2629126066095004 1107 36 -1.1397485622205892 1107 37 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1.6666810382600907 1108 25 2.358982307820888 1108 26 -0.3018746162920891 1108 27 0.1542170435665433 1108 28 0.8458076572243343 1108 29 1.602229605031166 1108 30 1.7189901225995428 1108 31 0.9346637135497531 1108 32 0.6260777488538345 1108 33 0.32185173146768875 1108 34 1.317557557549197 1108 35 0.9895873933905008 1108 36 1.3853139377794115 1108 37 0.8923675980505621 1108 38 0.9677583966878096 1108 39 0.602810826043914 1108 40 1.3782469012459515 1108 41 0.9919121031223668 1108 42 1.4505078866667738 1108 43 1.276008113880117 1108 44 1.3599034773728356 1108 45 0.7236271258296973 1108 46 1.3168200161939367 1108 47 1.028194991865341 1108 48 0.32530892851935533 1108 49 0.11337659811473344 1108 50 0.7810288777644878 1109 1 0.18626847745607478 1109 2 0.021632485471830165 1109 3 -0.3586130072972509 1109 4 -0.27300399679225507 1109 5 0.05652640294598399 1109 6 1.191129925794069 1109 7 0.6769815616214316 1109 8 -0.34056360584654044 1109 9 1.3311048438964619 1109 10 1.2681139010643356 1109 11 1.1613425411102511 1109 12 -0.6432054304627322 1109 13 1.751255790628285 1109 14 2.389355327637251 1109 15 1.9359034080198008 1109 16 0.9813248167555173 1109 17 0.1947729958814861 1109 18 0.7159480493695476 1109 19 1.2449593270264252 1109 20 0.9960515827375396 1109 21 0.8433774230115958 1109 22 0.15187876701418546 1109 23 -0.4045906801413537 1109 24 0.4180143715934257 1109 25 0.15764897448755377 1109 26 0.2221253837079118 1109 27 0.3268837102332114 1109 28 0.6261409905576656 1109 29 0.3142296050311657 1109 30 1.0663234559328778 1109 31 -0.31400295311691195 1109 32 2.3820777488538347 1109 33 0.8758517314676908 1109 34 -0.17277577578413528 1109 35 -0.29207927327616545 1109 36 0.3993139377794108 1109 37 -0.41396573528276903 1109 38 -0.12590826997885785 1109 39 0.4431441593772476 1109 40 0.8812469012459516 1109 41 0.8949121031223655 1109 42 0.727174553333441 1109 43 0.7386747805467841 1109 44 0.7339034773728379 1109 45 0.5822937924963618 1109 46 0.779486682860604 1109 47 0.6738616585320081 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2211 20 1.9405918126225963 2211 21 1.958319951747228 2211 22 1.368258077359016 2211 23 0.7362139175597981 2211 24 1.151922417570436 2211 25 0.34098230782088734 2211 26 1.6100564181906698 2211 27 0.7343549746010289 2211 28 1.6953938641208843 2211 29 0.3314709843415109 2211 30 0.8071280536340275 2211 31 0.8147326790669958 2211 32 1.261388093681422 2211 33 0.603437938364241 2211 34 1.2855575575491969 2211 35 1.2564839451146383 2211 36 1.5372449722621688 2211 37 1.775402080809183 2211 38 1.6588618449636705 2211 39 -0.04277538085263899 2211 40 -0.02823585737473877 2211 41 0.302670723812021 2211 42 -0.11535418229874139 2211 43 0.2513184587077042 2211 44 0.3494207187521461 2211 45 0.9276960913469381 2211 46 1.569716567918075 2211 47 1.1373674056584449 2211 48 0.5442054802434946 2211 49 1.2205490119078384 2211 50 0.5278564639713856 2212 1 -2.1187735393506486 2212 2 -0.5553983268530978 2212 3 -0.3679967607986505 2212 4 -1.6442925121984189 2212 5 -0.3200142133005137 2212 6 -1.1847804383515879 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2.137759016795872 2213 30 2.7426539881457614 2213 31 2.383335982457318 2213 32 1.9577080009546748 2213 33 2.2527424877702096 2213 34 1.8317760449441565 2213 35 2.3142932757434416 2213 36 2.027465198283611 2213 37 1.7939474299833371 2213 38 2.0779432706373875 2213 39 1.6818696495733256 2213 40 2.329776313010658 2213 41 1.8829373132064013 2213 42 2.3432809959104723 2213 43 2.380411475224655 2213 44 2.442727006784601 2213 45 2.592938050199443 2213 46 1.7472737977065407 2213 47 1.152363059092231 2213 48 2.36672069322524 2213 49 2.2166371023164153 2213 50 1.7061381214619686 2214 1 0.6515577336544212 2214 2 -0.20426283133257783 2214 3 -0.5642603902173597 2214 4 -0.6351252089134682 2214 5 -0.14340472653060132 2214 6 -1.3399995507899511 2214 7 -0.30242615187719224 2214 8 -0.31900713201733666 2214 9 -1.1113882690511971 2214 10 -0.3220376140871828 2214 11 -0.6049797729393376 2214 12 -0.9785360089751265 2214 13 -0.32367533884830024 2214 14 -0.23178792305145635 2214 15 -0.6943996222832283 2214 16 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2215 2 2.158887387432614 2215 3 0.4471292896215182 2215 4 1.0531864793982209 2215 5 0.2514143581280557 2215 6 1.7647993935811854 2215 7 0.2694885644225522 2215 8 1.1669293913523404 2215 9 1.402883555381056 2215 10 1.6749066181511694 2215 11 1.2094517848077295 2215 12 1.3448197796213037 2215 13 1.1215639138775835 2215 14 0.7016102295980318 2215 15 0.20705186740355686 2215 16 0.07726319210565791 2215 17 0.93062733761818 2215 18 1.3268724191174464 2215 19 1.3742310357099008 2215 20 1.4678302942221357 2215 21 0.9932373669891881 2215 22 0.7823409518881377 2215 23 0.6150171629959011 2215 24 1.1724961643105125 2215 25 0.0699739044595411 2215 26 0.9238060559768186 2215 27 0.02702376625562053 2215 28 0.15982446394702166 2215 29 0.6419606974681393 2215 30 1.2468556688180321 2215 31 0.7866973269951316 2215 32 0.8652710261647591 2215 33 0.7065240003752535 2215 34 0.9410197424231477 2215 35 -0.2403285729960558 2215 36 -0.3842995075987403 2215 37 0.49982978292451463 2215 38 -0.6027289982701731 2215 39 -1.2761135437039854 2215 40 -1.2164421743843015 2215 41 -0.38597024981881134 2215 42 -1.0684837099718791 2215 43 -0.8296725583887898 2215 44 -1.2631553461565765 2215 45 -1.566725815346775 2215 46 -0.3787766224615261 2215 47 -1.007300806453987 2215 48 -0.8013465336655194 2215 49 0.2250404636609531 2215 50 -0.7896601978657642 2216 1 0.427444948044311 2216 2 -0.1856504276934352 2216 3 -0.21673625659697038 2216 4 -0.9972336886690059 2216 5 0.07494376989276219 2216 6 -0.453688001376797 2216 7 0.47116923669145905 2216 8 -0.0011378355384152883 2216 9 0.3104465805911403 2216 10 0.4984360299158759 2216 11 0.4531492637993253 2216 12 -0.470306270798865 2216 13 -0.525494909651826 2216 14 0.6848035069089562 2216 15 0.08940480858002431 2216 16 -1.0739973120960258 2216 17 -0.41391047750786925 2216 18 -0.5638838834035624 2216 19 -1.4913151827774946 2216 20 -0.4481360923324864 2216 21 -0.1412164145234165 2216 22 -0.6546338380278307 2216 23 0.6906474150967412 2216 24 0.10526927355420668 2216 25 0.7086293666444163 2216 26 1.4196043753045515 2216 27 -0.9981863177779928 2216 28 -1.4284108301706269 2216 29 0.003305235283267649 2216 30 -1.4590266841231454 2216 31 -0.21330267300486838 2216 32 -1.3196029234150721 2216 33 -1.4363331424818888 2216 34 -0.6220054676608875 2216 35 0.6000075614577263 2216 36 -0.9305179949936999 2216 37 -2.037985343125907 2216 38 -0.03130042684160372 2216 39 -1.0408194260569275 2216 40 -0.7626606617792575 2216 41 -1.1506761321717498 2216 42 -0.5474753066105364 2216 43 -2.014546507968621 2216 44 -2.0026511444759016 2216 45 -0.9196669918173619 2216 46 -1.034238807335477 2216 47 -1.4862924030926408 2216 48 -1.1794977941697198 2216 49 -1.892606595162576 2216 50 -0.8652904499666043 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 2.0 5 4.0 6 19.0 7 638.0 8 2589.0 9 5220.0 10 4761.0 11 3714.0 12 3585.0 13 3565.0 14 3626.0 15 3535.0 16 3294.0 17 3123.0 18 2747.0 19 2550.0 20 2240.0 21 2102.0 22 1905.0 23 1805.0 24 1690.0 25 1669.0 26 1537.0 27 1474.0 28 1358.0 29 1278.0 30 1249.0 31 1215.0 32 1143.0 33 1054.0 34 1027.0 35 1080.0 36 1272.0 37 1771.0 38 1910.0 39 3684.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.025879230258795 18.23623092236231 20.176509621765096 28.561380225613803 2 21.189462938657545 15.757865012130612 42.543318882657864 20.50935316655398 3 21.307650497792743 26.26569273394933 30.273222594885528 22.153434173372396 4 18.417180353947106 21.256711075760588 32.65327765626036 27.672830914031948 5 20.020150064957445 27.21982130073972 35.941352705676486 16.818675928626348 6 28.037383177570092 26.25439119771989 24.401140054351426 21.307085570358588 7 31.598064558891757 24.309670577318222 20.686683900046397 23.40558096374362 8 32.559156890037784 24.163849671902963 21.843971631205676 21.433021806853585 9 30.121695787045628 16.50051700824562 18.7594453429488 34.61834186175995 10 27.490620815823313 22.48617978868665 28.585632282953082 21.437567112536954 11 37.10096510764662 17.802789267154523 23.354809629865308 21.741435995333546 12 27.534733269699863 21.05605048255382 25.19487750556793 26.214338742178384 13 31.19663785331707 15.968870976295275 25.114016015272846 27.72047515511481 14 35.57877152743713 18.246781656436024 20.38925053362854 25.78519628249831 15 28.75760669256367 22.44156601747385 20.85990427830883 27.940923011653652 16 32.19251763018447 19.11629945416152 21.760495106046722 26.93068780960729 17 38.817150129925224 18.89881741528345 18.886885506708385 23.397146948082938 18 33.47718701864344 16.68481436235194 20.040367557231637 29.797631061772982 19 32.0213599713075 17.41873779572535 22.87562598796509 27.68427624500206 20 36.24571383004173 17.773318093617927 21.9398740065389 24.041094069801442 21 29.95705986360192 20.120710971670146 19.69130960768934 30.230919557038593 22 32.931423265685105 16.922668788964053 18.767741079718796 31.378166865632046 23 39.473614425881735 18.971095200212147 18.611774065234684 22.94351630867144 24 30.994889493595274 25.8153580672994 18.095174885511383 25.09457755359395 25 35.918930390492356 20.01405984719864 17.505836162988114 26.56117359932088 26 34.908762995968594 23.713664332696798 17.74877997029493 23.62879270103968 27 31.701721348433807 24.24210275044426 20.19998408614699 23.856191814974935 28 35.976555766247195 22.356888069696208 18.780581597337328 22.885974566719266 29 32.82980331825838 22.06999907163035 21.24109759817509 23.85910001193618 30 35.740723543692546 20.51897743403335 19.984345772695313 23.755953249578795 31 33.75242036020264 19.442455107291583 21.970239516193203 24.834885016312562 32 32.19941115620275 20.506087371687755 21.959629718044617 25.33487175406488 33 35.55788384769433 19.262344000742697 22.592538560496543 22.58723359106643 34 31.888594164456237 21.255968169761275 23.20159151193634 23.653846153846153 35 32.98233515463371 22.424539812211552 21.39409049917776 23.199034533976977 36 35.9505557176583 19.07742910952545 23.060293376482136 21.91172179633412 37 32.24728487886382 20.352468472769225 23.432920926655264 23.967325721711685 38 33.543758205906926 18.256568041059374 25.066641910799305 23.133031842234395 39 32.83995808962983 18.83446730062733 25.719173994350058 22.606400615392776 40 27.545761270954543 29.625939799686513 23.777264153449696 19.051034775909248 41 31.74361082744261 15.847269930107027 26.217158923621703 26.19196031882866 42 26.613790999641896 19.128082019178482 31.17232781145138 23.08579916972824 43 27.08899704271487 15.738591907913058 29.233360298115556 27.939050751256516 44 27.410629044234646 15.610745730348999 31.608677203776388 25.36994802163997 45 26.515945103759197 15.300669628058078 34.9943645163429 23.18902075183982 46 26.3796441167891 19.155904425999097 28.41629319260654 26.048158264605263 47 29.99893921714225 18.098281531770446 30.59032566033733 21.312453590749975 48 30.755257365615634 20.526929910631413 26.294783738630034 22.423028985122915 49 28.43808008485813 20.722619994696366 27.073720498541505 23.765579421904004 50 29.96446377426541 22.07091333404052 23.35180863477246 24.61281425692161 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.0 6 1.0 7 2.0 8 3.0 9 3.0 10 3.0 11 4.0 12 5.0 13 10.5 14 16.0 15 24.5 16 33.0 17 29.5 18 26.0 19 36.0 20 46.0 21 81.5 22 117.0 23 145.5 24 174.0 25 207.0 26 240.0 27 299.5 28 359.0 29 465.5 30 572.0 31 637.5 32 703.0 33 957.0 34 1211.0 35 1274.5 36 1338.0 37 1528.5 38 1719.0 39 1881.0 40 2043.0 41 2315.0 42 2587.0 43 2877.0 44 3167.0 45 3416.5 46 3666.0 47 4048.5 48 4431.0 49 4625.0 50 4819.0 51 4965.5 52 5112.0 53 5183.5 54 5255.0 55 5257.0 56 5259.0 57 5013.0 58 4767.0 59 4462.0 60 4157.0 61 3939.0 62 3721.0 63 3537.5 64 3354.0 65 3107.0 66 2860.0 67 2710.0 68 2560.0 69 2441.5 70 2323.0 71 2120.5 72 1918.0 73 1804.5 74 1691.0 75 1580.0 76 1469.0 77 1383.5 78 1298.0 79 1067.5 80 837.0 81 724.5 82 612.0 83 497.5 84 383.0 85 319.0 86 255.0 87 199.0 88 143.0 89 116.5 90 90.0 91 66.5 92 43.0 93 32.5 94 22.0 95 16.5 96 11.0 97 10.5 98 10.0 99 7.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11267979054815404 2 0.007953867568104992 3 0.0026512891893683303 4 0.0 5 0.0013256445946841651 6 0.0 7 0.0 8 0.0 9 0.0013256445946841651 10 0.0026512891893683303 11 0.003976933784052496 12 0.003976933784052496 13 0.009279512162789156 14 0.010605156757473321 15 0.010605156757473321 16 0.18293895406641478 17 0.009279512162789156 18 0.168356863524889 19 0.20414926758136145 20 0.2558494067740439 21 0.28368794326241137 22 0.059654006760787436 23 0.019884668920262475 24 0.13256445946841652 25 0.0570027175714191 26 0.03579240405647246 27 0.038443693245840786 28 0.029164181083051632 29 0.04507191621926161 30 0.07423609730231324 31 0.04374627162457745 32 0.04374627162457745 33 0.04507191621926161 34 0.04639756081394578 35 0.04109498243520912 36 0.04904885000331412 37 0.03181547027241997 38 0.042420627029893285 39 0.04772320540862995 40 0.20282362298667728 41 0.04507191621926161 42 0.05037449459799828 43 0.03711804865115662 44 0.02518724729899914 45 0.026512891893683305 46 0.022535958109630806 47 0.02518724729899914 48 0.022535958109630806 49 0.019884668920262475 50 0.02518724729899914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 75435.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.81467488566315 #Duplication Level Percentage of deduplicated Percentage of total 1 98.16584393822744 86.2040167031219 2 0.6038373865917909 1.0605156757473322 3 0.19926633757529097 0.5249552594949294 4 0.061893332125658555 0.2174057135282031 5 0.08000845372341228 0.3512958175913038 6 0.08302764065637123 0.4374627162457745 7 0.05736455172622013 0.35262146218598794 8 0.05132617786030222 0.3605753297540929 9 0.07095089292453542 0.5607476635514018 >10 0.6159141343236266 9.270232650626367 >50 0.01056715426535634 0.6601710081527143 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 99 0.13123881487373235 No Hit CTGTCTCTTATACACATCTGACGCTGCCGACGAATGACCATGTGTAGATC 95 0.1259362364949957 No Hit GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG 86 0.1140054351428382 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.16437992974083648 0.0 2 0.0 0.0 0.0 0.16437992974083648 0.0 3 0.0 0.0 0.0 0.16437992974083648 0.0 4 0.0 0.0 0.0 0.16437992974083648 0.0 5 0.0 0.0 0.0 0.16968250811957314 0.0 6 0.0 0.0 0.0 0.16968250811957314 0.0 7 0.0 0.0 0.0 0.17100815271425732 0.0 8 0.0 0.0 0.0 0.17365944190362564 0.0 9 0.0 0.0 0.0 0.17763637568767812 0.0 10 0.0 0.0 0.0 0.19354411082388812 0.0 11 0.0 0.0 0.0 0.22668522569099225 0.0 12 0.0 0.0 0.0 0.2704314973155697 0.0 13 0.0 0.0 0.0 0.3353880824550938 0.0 14 0.0 0.0 0.0 0.39769337840524954 0.0 15 0.0 0.0 0.0 0.4706038311128786 0.0 16 0.0 0.0 0.0 0.49579107841187775 0.0 17 0.0 0.0 0.0 0.5859349108504011 0.0 18 0.0 0.0 0.0 0.67475309869424 0.0 19 0.0 0.0 0.0 0.9359050838470206 0.0 20 0.0 0.0 0.0 1.0737721216941738 0.0 21 0.0 0.0 0.0 1.0843772784516472 0.0 22 0.0 0.0 0.0 1.1281235500762246 0.0 23 0.0 0.0 0.0 1.1904288460263803 0.0 24 0.0 0.0 0.0 1.228872539272221 0.0 25 0.0 0.0 0.0 1.228872539272221 0.0 26 0.0 0.0 0.0 1.2328494730562736 0.0 27 0.0 0.0 0.0 1.2421289852190627 0.0 28 0.0 0.0 0.0 1.2580367203552727 0.0 29 0.0 0.0 0.0 1.2779213892755352 0.0 30 0.0 0.0 0.0 1.332272817657586 0.0 31 0.0 0.0 0.0 1.435673096042951 0.0 32 0.0 0.0 0.0 1.541724663617684 0.0 33 0.0 0.0 0.0 1.6278915622721548 0.0 34 0.0 0.0 0.0 1.7525021541724664 0.0 35 0.0 0.0 0.0 1.9964207595943528 0.0 36 0.0 0.0 0.0 2.1342877974415058 0.0 37 0.0 0.0 0.0 2.4763041028700203 0.0 38 0.0 0.0 0.0 3.0118645191224234 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTTGA 20 7.8515825E-4 43.952255 6 ACGCGGC 30 1.277703E-4 36.724293 14 TCGGGCC 25 0.0023360855 35.2085 12 CTCGTTG 25 0.0023513644 35.161804 41 ATATCGG 25 0.0023513644 35.161804 42 CAGATGT 25 0.0023513644 35.161804 5 ACATCTT 25 0.0023513644 35.161804 5 TTTACAC 70 2.7284841E-11 34.533913 2 CTTTACA 70 1.0077201E-9 31.415298 1 GCACGAC 30 0.00570457 29.340418 35 CTATACA 45 4.0313196E-5 29.320944 1 ATGGATC 30 0.005741737 29.301506 7 ACATTTG 30 0.005741737 29.301506 5 GATATCG 30 0.005741737 29.301506 41 CATCGGA 30 0.005741737 29.301506 9 TATCGGG 30 0.005741737 29.301506 43 TTCTCGG 30 0.005741737 29.301506 42 TCTCGGG 135 0.0 27.673643 43 CCCATCT 40 7.0151314E-4 27.47016 7 TACACGT 40 7.0151314E-4 27.47016 2 >>END_MODULE