FastQCFastQC Report
Wed 25 May 2016
SRR1780599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75435
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4006353.10929939683171No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT73639.760721150659508No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG64788.587525684364023No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC43135.717505136872805No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC11561.532445151454895No Hit
TATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCT7601.0074898919599655No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC7100.9412076622257572No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC4730.6270298932856101No Hit
ACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCT3120.41360111354145956Illumina PCR Primer Index 8 (95% over 22bp)
ATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCTGCTT2710.35924968515940875RNA PCR Primer, Index 39 (96% over 27bp)
CATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCTGCT2380.3155034135348313Illumina PCR Primer Index 8 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC2350.3115264797507788No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATATCGTATGCCGT1820.24126731623251807No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAAATAATCTCGTATGCCGTC1790.23729038244846554No Hit
CTTAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC1460.19354411082388812No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG1240.16437992974083648No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG1180.15642606217273147No Hit
TCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTTCTGCTTG1170.15510041757804732Illumina PCR Primer Index 8 (96% over 27bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTA1130.14979783919931067No Hit
CTTATCACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC1030.136541393252469No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG950.1259362364949957No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG900.11930801352157487No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC880.11665672433220653No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG840.11135414595346987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATAT254.4032633E-544.034
CTTTACA1250.044.01
TTCCGAG207.8097143E-444.012
TATCGTA254.4032633E-544.038
AAATATC254.4032633E-544.035
TTATCAC302.4962665E-644.02
GAGCTGG207.8097143E-444.01
ATAAATA254.4032633E-544.033
CTTATCA302.4962665E-644.01
TATCACA302.4962665E-644.03
AATATCG254.4032633E-544.036
ATCGTAT254.4032633E-544.039
TCTTTAT454.674803E-1044.01
TTTACAC1250.044.02
ATATCGT254.4032633E-544.037
AGCTGGA207.8097143E-444.02
TTACACA1400.039.2857133
TCACATC357.2001094E-637.7142875
CCGTCTT8350.037.41317444
CTTTATA553.3342076E-936.02
Produced by FastQC (version 0.11.5)