FastQCFastQC Report
Wed 25 May 2016
SRR1780598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90334
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTC150.002218949669.994463
GGAATGA200.006933583452.49584640
GCCCACG200.006933583452.49584665
GAATGTG200.006933583452.4958465
CGTGCGA200.006933583452.49584610
TATGGGT200.006933583452.4958466
ATAGACA200.006933583452.4958464
AGTGGCG200.006933583452.49584651
ATCCCTT200.006933583452.4958462
TACAGCG200.006933583452.49584666
CCCGCTA200.006933583452.49584617
TAGCCCG350.001250121339.9968345
CCGCTAA400.002408621834.9972318
GATGGCG400.002408621834.997237
CCCTAAG552.8777827E-431.9928111
TGCGGTT450.00428933131.1086541
GTAGAAA450.00428933131.1086568
TGTTCTG450.00428933131.1086525
CTATTGC450.00428933131.1086520
CGATCAA450.00428933131.1086546
Produced by FastQC (version 0.11.5)