FastQCFastQC Report
Wed 25 May 2016
SRR1780598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90334
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT10261.1357849757566365No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT5250.5811765226824894TruSeq Adapter, Index 12 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1750.19372550756082982No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1400.15498040604866384No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG930.10295126973232671No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCAAC150.002218253470.012
CGGGACG150.002218253470.054
CGAGAAT207.889184E-570.023
GTCTAAG252.379217E-455.9999961
GATCGTT200.006931415352.556
GATGTAG200.006931415352.51
TCGGGAC200.006931415352.553
CGGCTGC200.006931415352.567
TTCGGCA200.006931415352.567
CCGGTGG200.006931415352.563
CCTGGCG200.006931415352.530
ATACGGG200.006931415352.56
GGATAGC305.8505125E-446.66666470
AGAATAC305.8505125E-446.66666425
CTATGTG350.001249635140.024
GAATACT350.001249635140.026
CTAGGGA350.001249635140.04
GCCGTCT3350.035.5223946
GAAAGGG400.002407687335.042
ATCCAAT400.002407687335.044
Produced by FastQC (version 0.11.5)