FastQCFastQC Report
Wed 25 May 2016
SRR1780597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630244
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATAAG250.002330800335.26482815
CGCGGTT250.002356401435.186425
TCGCTTA250.002362836435.1668542
TCCGTAT502.337034E-630.83708813
TTCGGCA454.0157436E-529.38735815
TGACGGT300.00570926629.36865613
GTAGGTC602.9208968E-729.3569834
TAGATTC300.00572037429.3569835
TATTCCG454.077216E-529.32225
TTCCGAA454.0904924E-529.30803327
CGTGCGA852.6193447E-1028.4822110
CGCTATT703.5213816E-828.3378116
CCTGGTA554.9251157E-628.0225735
CCGTACG406.9790875E-427.53311513
TATACCG407.090167E-427.4588436
CGGATAC656.001628E-727.03643
GCGAGTA508.289624E-526.4212837
ATGGTGC757.103699E-826.3604876
GCGAACT959.713403E-1025.45171428
TTCAACG801.2975761E-724.7699536
Produced by FastQC (version 0.11.5)