FastQCFastQC Report
Wed 25 May 2016
SRR1780597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630244
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT113201.796129752921091No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT47060.7466949308521779TruSeq Adapter, Index 7 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC18050.28639701448962623No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC12440.19738387037401386No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG9980.15835136867625874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT6200.036.9008344
TCGTTGT301.3009248E-436.66428834
AAGCGGC301.3009248E-436.6642888
TACGACG250.00234994435.20609713
ACGACGT250.00234994435.20609714
TCTTCGC250.002352690335.19771629
GCCGTCT6950.032.91872843
ATAAAGA2200.032.010621
TGGCGTG2200.029.9980523
GCGTGAA2200.029.9980525
ATTGGCG2350.029.95550321
GACGATT300.00573809529.33841314
TATGGAA300.005740321229.3360863
CTTATGC300.005740321229.3360863
GTGCAGA454.0660885E-529.33375716
ATGATTC2100.029.3314340
CGTAGTG454.0682906E-529.3314343
GCTAGTC1050.029.331437
GGCTCGT454.0682906E-529.3314339
TTCAGCC1950.029.33142944
Produced by FastQC (version 0.11.5)