FastQCFastQC Report
Wed 25 May 2016
SRR1780596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87592
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGC207.891029E-569.994292
CCAAGTC150.00221874569.99428662
AAGGCCA150.00221874569.99428625
GTACATG252.3132998E-456.317061
GCGAACT252.3797738E-455.9954328
TGGGTCG252.3797738E-455.9954314
ACCAGTC252.3797738E-455.9954318
TAAGACT252.3797738E-455.995439
AAAGCGA252.3797738E-455.9954325
CGTTGCA200.006932948352.4957253
ATTGTCT200.006932948352.495728
TACTGTA200.006932948352.4957211
CAAGGCC200.006932948352.4957224
CCAAGGC200.006932948352.4957223
ACCAAGG200.006932948352.4957222
GCGTCAT200.006932948352.495723
CGTCATA200.006932948352.495724
CCGTTGC200.006932948352.4957252
AGACTTC305.851882E-446.66285711
GGGTCGC305.851882E-446.66285715
Produced by FastQC (version 0.11.5)