##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780596_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87592 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.905664900904192 34.0 31.0 34.0 12.0 34.0 2 31.481927573294364 34.0 32.0 34.0 23.0 34.0 3 31.521874143757422 34.0 33.0 34.0 23.0 34.0 4 31.929000365330168 34.0 34.0 34.0 27.0 34.0 5 31.944903644168416 34.0 34.0 34.0 27.0 34.0 6 34.52864416841721 38.0 35.0 38.0 25.0 38.0 7 34.14383733674308 37.0 34.0 38.0 24.0 38.0 8 32.88018312174628 37.0 32.0 38.0 21.0 38.0 9 33.75069641063111 37.0 34.0 38.0 21.0 38.0 10-11 34.148118549639236 37.0 34.0 38.0 23.5 38.0 12-13 34.52803338204402 38.0 35.5 38.0 25.0 38.0 14-15 34.155796191433005 38.0 34.0 38.0 23.5 38.0 16-17 34.068276783267876 38.0 34.0 38.0 23.0 38.0 18-19 34.020133117179654 37.5 34.0 38.0 22.5 38.0 20-21 34.07232395652571 38.0 34.0 38.0 23.0 38.0 22-23 34.102183989405425 37.5 34.0 38.0 23.5 38.0 24-25 34.53055073522697 38.0 35.5 38.0 25.0 38.0 26-27 34.58250182665084 38.0 35.5 38.0 25.0 38.0 28-29 34.70999634669833 38.0 36.0 38.0 26.0 38.0 30-31 34.554143072426704 38.0 36.0 38.0 25.0 38.0 32-33 34.28642455932049 38.0 34.5 38.0 24.0 38.0 34-35 34.16925061649465 37.0 34.0 38.0 24.0 38.0 36-37 34.14375742076902 37.5 34.5 38.0 23.5 38.0 38-39 34.68083843273358 38.0 36.0 38.0 26.0 38.0 40-41 34.17854370262124 37.5 34.5 38.0 23.5 38.0 42-43 34.52360375376747 38.0 35.5 38.0 25.0 38.0 44-45 33.538724997716685 37.0 34.0 38.0 22.0 38.0 46-47 34.30138597132158 37.0 34.5 38.0 24.0 38.0 48-49 34.013956754041466 37.0 34.0 38.0 23.5 38.0 50-51 33.9920597771486 37.0 34.0 38.0 23.5 38.0 52-53 33.98917709379852 37.0 34.0 38.0 23.5 38.0 54-55 33.68679788108503 37.0 34.0 38.0 22.5 38.0 56-57 33.802305005023285 37.0 34.0 38.0 23.0 38.0 58-59 33.663416750388166 37.0 33.5 38.0 22.5 38.0 60-61 33.67568385240661 37.0 34.0 38.0 22.5 38.0 62-63 33.81871061284136 37.0 34.0 38.0 23.0 38.0 64-65 33.84173212165494 37.0 34.0 38.0 23.0 38.0 66-67 33.76900858525893 37.0 34.0 38.0 23.0 38.0 68-69 33.324236231619324 37.0 32.5 38.0 21.5 38.0 70-71 33.187933829573474 37.0 32.5 38.0 21.0 38.0 72-73 33.32186158553293 37.0 33.0 38.0 21.5 38.0 74-75 32.90541373641429 37.0 31.5 38.0 20.0 38.0 76 32.148997625353914 36.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.828838917624605 1101 2 -0.3615256129374167 1101 3 0.29176336189638974 1101 4 -1.6122306410516138 1101 5 -0.7119073204804955 1101 6 -1.9890722717937237 1101 7 -0.4012464041846968 1101 8 0.38763813353978804 1101 9 -0.2533358959583296 1101 10-11 -0.46939994462034207 1101 12-13 -0.965412910893626 1101 14-15 -0.19854828662803925 1101 16-17 -1.5451610751830813 1101 18-19 0.5958725409269121 1101 20-21 -0.658867324176164 1101 22-23 0.4319425888660149 1101 24-25 -1.050896074063914 1101 26-27 -0.6262202595121593 1101 28-29 0.6767720130623189 1101 30-31 -0.6156915932040903 1101 32-33 0.5045151368469547 1101 34-35 -0.9503196376922034 1101 36-37 0.8269901366066108 1101 38-39 -0.2409175605461158 1101 40-41 0.4871763956129769 1101 42-43 0.15700612785192902 1101 44-45 0.9761936180907895 1101 46-47 1.1467890630187938 1101 48-49 -0.2962341561017112 1101 50-51 -0.7605137098638153 1101 52-53 0.3894613794912871 1101 54-55 -0.6468933345469523 1101 56-57 0.27607483915299014 1101 58-59 0.10944046608619828 1101 60-61 0.49539131044930684 1101 62-63 -1.5678420097395431 1101 64-65 0.16672118191680596 1101 66-67 -1.31988748592034 1101 68-69 0.6251868647818029 1101 70-71 0.8990369932997311 1101 72-73 -0.08405207449654739 1101 74-75 -1.691345395601381 1101 76 -1.1532600299927545 1102 1 0.5316661328804457 1102 2 -1.1624347038465075 1102 3 -0.061064920931894306 1102 4 -0.1971801360011085 1102 5 0.2689007603275897 1102 6 0.2763822736608219 1102 7 1.1413798584415673 1102 8 1.0813755072771585 1102 9 0.2977752151527824 1102 10-11 -0.6758140860344852 1102 12-13 -4.027231092711816 1102 14-15 -1.382841215920969 1102 16-17 -2.9004136004356056 1102 18-19 -1.0069052368508693 1102 20-21 -0.42715015245898513 1102 22-23 -1.0116432697198476 1102 24-25 0.14708372391588398 1102 26-27 -6.331220259512165 1102 28-29 -0.6705007142104051 1102 30-31 0.2740558815433829 1102 32-33 -7.564828297496479 1102 34-35 -1.0686529710255428 1102 36-37 -0.3391209745045032 1102 38-39 0.2476177929892387 1102 40-41 -0.061358957922379886 1102 42-43 -0.03395346810766853 1102 44-45 -6.20345284655567 1102 46-47 -0.5289180076882758 1102 48-49 -0.5932038530714081 1102 50-51 -6.4649076492577535 1102 52-53 -2.1566497316198223 1102 54-55 0.7484096957560809 1102 56-57 -1.526399908321757 1102 58-59 0.5922182438639751 1102 60-61 -0.1404672754092786 1102 62-63 -4.819609686507221 1102 64-65 -1.4505515453559212 1102 66-67 -0.14862485965771555 1102 68-69 -7.678045458450519 1102 70-71 -0.6609125016497686 1102 72-73 -0.05364803409250385 1102 74-75 -6.394274688530679 1102 76 -2.6187145754472994 1103 1 0.6235179847322989 1103 2 -0.12918367503992556 1103 3 0.7149021572574057 1103 4 0.7687457899248145 1103 5 0.4935921183522751 1103 6 -1.3185148456807454 1103 7 0.8868942617337439 1103 8 1.2403878529561752 1103 9 0.8791743921075152 1103 10-11 0.8222106053235407 1103 12-13 -0.8982598992961783 1103 14-15 -0.7143638496658227 1103 16-17 -0.8228827362380713 1103 18-19 -0.2320081175093094 1103 20-21 -0.23447525533964608 1103 22-23 -0.20966796107786934 1103 24-25 0.6742442177430519 1103 26-27 -0.6347593541623695 1103 28-29 0.6466186273533836 1103 30-31 0.5875538239302145 1103 32-33 -5.095774799554093 1103 34-35 -0.9502167570337718 1103 36-37 -0.07105513088311 1103 38-39 0.7555190275571348 1103 40-41 0.9039085317895541 1103 42-43 0.9234950915631117 1103 44-45 -5.122300583181186 1103 46-47 -0.15221018875823233 1103 48-49 0.49980026215492757 1103 50-51 0.7277672478615926 1103 52-53 0.14203339595218978 1103 54-55 0.5536771854680183 1103 56-57 1.508332601966309 1103 58-59 0.3632470504483436 1103 60-61 0.9834010373479245 1103 62-63 -0.04553561243314874 1103 64-65 0.8322468085535419 1103 66-67 -0.23693761685936465 1103 68-69 -3.1507203555698666 1103 70-71 0.6190051938234831 1103 72-73 1.594417809528899 1103 74-75 -1.3038220136335568 1103 76 0.6848245192029054 1104 1 -2.184683073468758 1104 2 -0.29291089432269857 1104 3 0.2319509520839773 1104 4 0.1682166893957131 1104 5 -0.45173416030733193 1104 6 -0.9848875676090216 1104 7 1.0589989060606158 1104 8 1.2320104279120798 1104 9 0.039521246898814866 1104 10-11 -0.23819503841543366 1104 12-13 -0.31191363239435077 1104 14-15 -0.02307931115905859 1104 16-17 -0.3100961401181479 1104 18-19 0.6049995250538984 1104 20-21 0.22951651420768115 1104 22-23 -0.3119607300373062 1104 24-25 0.1308138826460521 1104 26-27 0.48766862937672073 1104 28-29 -0.37748484119452996 1104 30-31 -0.8151504676629671 1104 32-33 -0.6433203609885396 1104 34-35 -0.3427799551525226 1104 36-37 -0.2162638316473604 1104 38-39 -0.284604429232985 1104 40-41 0.594752153188729 1104 42-43 0.5007290715748667 1104 44-45 -3.4094845925874147 1104 46-47 0.2193359605656866 1104 48-49 0.45798662311906213 1104 50-51 0.8405685412184383 1104 52-53 -0.3149036998737884 1104 54-55 -0.44397125662487014 1104 56-57 0.5496318377099882 1104 58-59 0.5467420533877885 1104 60-61 0.08334224840024973 1104 62-63 0.4399141230165853 1104 64-65 0.006115121310749316 1104 66-67 0.002406886374032524 1104 68-69 -3.8746327600378265 1104 70-71 -0.5387696445069139 1104 72-73 0.6198440293995588 1104 74-75 -0.7426080218640081 1104 76 0.34414256740984683 1105 1 -0.663369709413459 1105 2 0.42982336066961935 1105 3 -0.21050936537633902 1105 4 0.012622731382403884 1105 5 0.6978434126573347 1105 6 -0.04281127472627588 1105 7 1.3975805752874422 1105 8 1.0138844678506373 1105 9 1.4248899104932846 1105 10-11 1.0726088530336142 1105 12-13 -1.0645698023892294 1105 14-15 0.08044731454497622 1105 16-17 1.0297656110339233 1105 18-19 0.3910338312494872 1105 20-21 0.015582822451406741 1105 22-23 0.5721918557281853 1105 24-25 0.629565802768937 1105 26-27 0.7620861921007318 1105 28-29 -0.7585652303394355 1105 30-31 0.09834799623872215 1105 32-33 0.8695444623601531 1105 34-35 0.36177713650134535 1105 36-37 0.8951352978969283 1105 38-39 0.1766410904802811 1105 40-41 0.4240532284575522 1105 42-43 0.6460823741862356 1105 44-45 1.440489805774078 1105 46-47 1.2145676553941627 1105 48-49 0.4139198028425639 1105 50-51 -0.08243453097818332 1105 52-53 -0.2738450721216168 1105 54-55 -0.37801503542671355 1105 56-57 0.26753378343452283 1105 58-59 0.0037953047958794173 1105 60-61 -0.03074505318705434 1105 62-63 0.26793511636016376 1105 64-65 0.06122264819246226 1105 66-67 0.4974145668655865 1105 68-69 1.126103287069192 1105 70-71 1.6932452044434214 1105 72-73 0.46517813078204284 1105 74-75 -0.9614700290324691 1105 76 -0.770488768995687 1106 1 -0.5743935832233227 1106 2 -0.22358007829584636 1106 3 -1.409297911631846 1106 4 -0.8953103366863786 1106 5 -0.2795084418261098 1106 6 -0.46300098625107466 1106 7 0.2310323302966779 1106 8 0.12035739665552825 1106 9 0.9440209322355031 1106 10-11 -0.603851286230281 1106 12-13 -0.323442546456306 1106 14-15 -0.47366843080104815 1106 16-17 0.2781669184092266 1106 18-19 -0.5696560934343182 1106 20-21 -0.6051628788417602 1106 22-23 -0.5038703867046692 1106 24-25 -0.35458049536947556 1106 26-27 -0.41553744012890803 1106 28-29 -1.7482980710385974 1106 30-31 -1.0153812207571562 1106 32-33 0.47040420372721314 1106 34-35 0.05287419490691292 1106 36-37 0.0405363921132178 1106 38-39 0.08238039700294308 1106 40-41 -0.042729491451503065 1106 42-43 0.5444410497582197 1106 44-45 0.21148596891178073 1106 46-47 0.32499290763232835 1106 48-49 0.18902815867601674 1106 50-51 0.034270030624767855 1106 52-53 -0.04060469980386472 1106 54-55 0.07522320530087256 1106 56-57 0.7373984274589631 1106 58-59 0.03708363789237268 1106 60-61 0.6628122155354106 1106 62-63 0.40657729342425597 1106 64-65 0.4661004370229307 1106 66-67 0.49970602629431937 1106 68-69 1.0334768127193215 1106 70-71 0.273996945242061 1106 72-73 0.4651870124077391 1106 74-75 -1.3372604937191248 1106 76 -1.0388026811741717 1107 1 -0.033779629087053564 1107 2 0.5319526479321475 1107 3 0.9915657334378878 1107 4 0.8674136277230886 1107 5 0.5303851696380804 1107 6 1.0599395859137815 1107 7 0.6193069554990984 1107 8 1.1797329951998634 1107 9 -0.4580789789622841 1107 10-11 0.7241771304784734 1107 12-13 1.1254961800154604 1107 14-15 0.5098509228581278 1107 16-17 1.4949575018920171 1107 18-19 1.6001215527846142 1107 20-21 1.4178125177210674 1107 22-23 -0.2835712451260832 1107 24-25 0.2862580761337199 1107 26-27 0.9989773689463348 1107 28-29 -0.39279320432898146 1107 30-31 0.42492105501725774 1107 32-33 1.5437443858588153 1107 34-35 1.1693443939283483 1107 36-37 0.04052768601371781 1107 38-39 0.7451496331297776 1107 40-41 1.11533845094894 1107 42-43 0.7689626495910602 1107 44-45 0.6964505350868393 1107 46-47 0.8909590234931031 1107 48-49 1.0488251324358373 1107 50-51 1.469427001598632 1107 52-53 1.2555680988588804 1107 54-55 0.825932752409571 1107 56-57 1.1291380802597146 1107 58-59 1.356179596520981 1107 60-61 0.4023083064967423 1107 62-63 -0.03375110064863662 1107 64-65 0.4234405495057345 1107 66-67 1.5584721978741243 1107 68-69 1.7477165090505764 1107 70-71 0.9147484557503134 1107 72-73 0.912192984791993 1107 74-75 0.5274293079559271 1107 76 -0.33824026714690447 1108 1 -2.3943469390149765 1108 2 -0.5357680371798423 1108 3 -0.820803483023397 1108 4 -0.8878990902494763 1108 5 -0.795151527254113 1108 6 -1.15734321653526 1108 7 0.338504041448104 1108 8 0.6685650497608222 1108 9 -0.4141202096838228 1108 10-11 -0.1423500337469079 1108 12-13 0.13120027983720917 1108 14-15 -0.10545559500592816 1108 16-17 -0.9530933389977037 1108 18-19 -0.2879509884848517 1108 20-21 -0.21394753807989986 1108 22-23 -1.0470681063211558 1108 24-25 -1.6280796747769202 1108 26-27 -1.4041614359827506 1108 28-29 -0.5799124789162846 1108 30-31 -0.2731336609402817 1108 32-33 -0.34339038899975094 1108 34-35 -0.8419863043588691 1108 36-37 -1.1068333928051572 1108 38-39 0.060820407368318286 1108 40-41 -0.5165223566151909 1108 42-43 0.24421646653284768 1108 44-45 0.3058281991959646 1108 46-47 0.6674218615927714 1108 48-49 0.11660006849721327 1108 50-51 -0.21314294337540218 1108 52-53 -0.4208327381557737 1108 54-55 -0.360217755224312 1108 56-57 0.3078484576913141 1108 58-59 -1.594971298619683 1108 60-61 0.4641078879894138 1108 62-63 -0.3503286407555848 1108 64-65 -0.2634927218265162 1108 66-67 -0.5032653825335274 1108 68-69 0.37777153501352956 1108 70-71 1.3346430539057863 1108 72-73 0.2919728809401718 1108 74-75 0.8670325010118063 1108 76 0.5875599343566194 1109 1 -0.5452062951030925 1109 2 -0.4625170083732577 1109 3 0.6943260255701631 1109 4 0.2749186294309851 1109 5 0.6705468508625643 1109 6 0.8131723971176115 1109 7 -1.1336818699534987 1109 8 0.7918281821742781 1109 9 1.3359645155642994 1109 10-11 1.2995768604675746 1109 12-13 0.3363080019383915 1109 14-15 0.6992163972477599 1109 16-17 -0.6953107197771686 1109 18-19 0.9181976438075736 1109 20-21 1.4836317405451283 1109 22-23 1.004323808469458 1109 24-25 1.3388532712409926 1109 26-27 1.503100728142158 1109 28-29 0.5766597796167616 1109 30-31 0.8077184329837124 1109 32-33 1.3795338424212176 1109 34-35 0.43249929234895035 1109 36-37 0.5626897250839704 1109 38-39 0.8275354884624946 1109 40-41 0.6940319885796811 1109 42-43 1.2835773960898607 1109 44-45 0.18428377895872927 1109 46-47 -0.20982335748252012 1109 48-49 0.2014463526899064 1109 50-51 1.487026507120845 1109 52-53 1.0185766058287342 1109 54-55 0.3191092842334484 1109 56-57 0.7881679929128111 1109 58-59 0.30769149489278647 1109 60-61 1.0348413665660345 1109 62-63 -0.0229018675771826 1109 64-65 0.038008125425967876 1109 66-67 0.3824039469266509 1109 68-69 1.5488681217963887 1109 70-71 -0.07441044403660158 1109 72-73 -0.26566449499785705 1109 74-75 1.9657253114693205 1109 76 -0.2164100486983287 1110 1 -0.10234595452076078 1110 2 -0.13799955740299552 1110 3 0.1634446095190576 1110 4 0.19474919919182554 1110 5 0.5474688681843958 1110 6 0.5530349933260936 1110 7 0.8127513135787083 1110 8 2.5455689056964985 1110 9 -1.3721643478411778 1110 10-11 -0.7747727099303461 1110 12-13 0.3381245558237538 1110 14-15 0.3082698951901435 1110 16-17 0.3768434892901027 1110 18-19 0.44409429824908386 1110 20-21 -0.1483309985770731 1110 22-23 0.34129024957351106 1110 24-25 0.37242077644959437 1110 26-27 -0.6951533139054717 1110 28-29 0.6846457293042363 1110 30-31 0.15097824323562037 1110 32-33 1.5669057796769295 1110 34-35 0.8606636259061204 1110 36-37 -0.0074240893348118675 1110 38-39 0.21179259540672035 1110 40-41 0.6210771183212245 1110 42-43 0.4579665690843342 1110 44-45 1.7456591943555395 1110 46-47 0.17971983517550427 1110 48-49 0.7769495639439299 1110 50-51 0.6656083897938458 1110 52-53 0.4265952707046736 1110 54-55 -0.036918058776691964 1110 56-57 0.0914894454671824 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46-47 0.9086933481609663 2116 48-49 0.6802175859887925 2116 50-51 0.7545343478053894 2116 52-53 1.6157829653943736 2116 54-55 0.11487078239338189 2116 56-57 0.40920459485983685 2116 58-59 -0.2228282563807582 2116 60-61 -1.654368401204671 2116 62-63 0.6973261921026719 2116 64-65 1.206188542749807 2116 66-67 0.2992654976599596 2116 68-69 0.3792917906928963 2116 70-71 -2.3207167111456073 2116 72-73 0.9255394353152298 2116 74-75 -0.7954102734548059 2116 76 -0.6158951921873879 2117 1 0.29205349271734704 2117 2 0.33793226863055636 2117 3 0.7034814603117354 2117 4 -0.06798543671466817 2117 5 -0.6452684547590586 2117 6 -0.6390305703758798 2117 7 -2.0821471548102153 2117 8 -2.379501149195825 2117 9 0.4173063058765294 2117 10-11 0.21902791192677995 2117 12-13 0.4422184485725893 2117 14-15 -0.4921888204061844 2117 16-17 0.37306244441658976 2117 18-19 0.40527621065167807 2117 20-21 0.30418521961032496 2117 22-23 0.6572762002087984 2117 24-25 0.6508553854857198 2117 26-27 0.8877705661759094 2117 28-29 0.8779396527620733 2117 30-31 0.31744018327631096 2117 32-33 1.3423174721263536 2117 34-35 0.21779962836284028 2117 36-37 -0.8833309643108223 2117 38-39 0.7179439907466261 2117 40-41 -1.194620935496289 2117 42-43 -0.9458087178528274 2117 44-45 0.3599926172567649 2117 46-47 -0.3852584765975564 2117 48-49 -0.2502374922151418 2117 50-51 0.7215755311703873 2117 52-53 0.7644613794912871 2117 54-55 -0.3600918332959111 2117 56-57 -0.039417237577616504 2117 58-59 -0.47289898345508163 2117 60-61 1.1234980660790015 2117 62-63 -0.5694058129088475 2117 64-65 -0.20412952700730358 2117 66-67 -1.3933445334599526 2117 68-69 0.1691818606116584 2117 70-71 1.157731738921072 2117 72-73 1.0405517620338998 2117 74-75 -0.39793421962139774 2117 76 -0.4959622818693177 2118 1 -0.4444095172377551 2118 2 -0.1769027889528907 2118 3 -0.5046583015465522 2118 4 -0.2583621690980351 2118 5 -0.830862833053029 2118 6 0.47978652897997165 2118 7 0.05097796718860792 2118 8 -4.368648133384777 2118 9 -1.9341869597881178 2118 10-11 -0.738887372086495 2118 12-13 0.19744975835201473 2118 14-15 -1.43641095587369 2118 16-17 -0.49109917963182426 2118 18-19 -1.0674253314135171 2118 20-21 -3.046488213924711 2118 22-23 -0.9804848772848587 2118 24-25 -0.31627325007938367 2118 26-27 0.061545697934640486 2118 28-29 -1.173904969529552 2118 30-31 -0.9983318253124125 2118 32-33 -0.8755138766926933 2118 34-35 0.5182973835843896 2118 36-37 -0.7331162463721128 2118 38-39 -0.9121458003913787 2118 40-41 -1.162871960286445 2118 42-43 -0.9297927116064884 2118 44-45 -0.18449303568096553 2118 46-47 -1.2089652890121556 2118 48-49 -1.4151896686742518 2118 50-51 -0.9816452379102358 2118 52-53 -1.7983045779555198 2118 54-55 -0.41995909857016045 2118 56-57 1.0093920538531833 2118 58-59 0.3429511043840705 2118 60-61 -2.422500372335989 2118 62-63 -0.12717469832754347 2118 64-65 -0.8531047368452889 2118 66-67 -3.1088139849532226 2118 68-69 0.513774872518745 2118 70-71 -1.4625200666615896 2118 72-73 -2.271189405250894 2118 74-75 0.23002791194213756 2118 76 -0.5370124477877241 2119 1 -0.40986751344193095 2119 2 0.7355934651675788 2119 3 0.7384342965954929 2119 4 0.1448542927938803 2119 5 0.5972262689347829 2119 6 1.3610771093415721 2119 7 -1.2018439287258857 2119 8 1.0433786371676135 2119 9 0.5363354655440133 2119 10-11 0.5799918607573744 2119 12-13 0.5828158556449949 2119 14-15 -0.18703526912910462 2119 16-17 -1.0024167303260612 2119 18-19 -0.9695030459275529 2119 20-21 -1.5776039865747933 2119 22-23 0.7884193280892333 2119 24-25 0.1248615016936725 2119 26-27 0.6351647170136587 2119 28-29 1.0546636050384208 2119 30-31 0.7680151929674892 2119 32-33 -0.14927274194091922 2119 34-35 0.9758540712279853 2119 36-37 -0.2292305206703844 2119 38-39 0.454440954178601 2119 40-41 -0.6640506637126791 2119 42-43 -0.8450958781233169 2119 44-45 -1.7370052722207703 2119 46-47 -0.17693052725009295 2119 48-49 -0.44484704555967625 2119 50-51 0.8431674681131369 2119 52-53 -1.5958672590063614 2119 54-55 -1.7779752807697378 2119 56-57 0.2398285736813719 2119 58-59 2.015191639795127 2119 60-61 0.6080460266251535 2119 62-63 0.10026511787462766 2119 64-65 0.8946127738929093 2119 66-67 0.4615785832217867 2119 68-69 -0.051723079733783095 2119 70-71 -1.1719297160472593 2119 72-73 -2.176433636596414 2119 74-75 -0.4841964412693258 2119 76 0.9125530301865012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 9.0 13 70.0 14 215.0 15 476.0 16 657.0 17 708.0 18 722.0 19 675.0 20 622.0 21 609.0 22 583.0 23 682.0 24 750.0 25 885.0 26 1046.0 27 1267.0 28 1577.0 29 1933.0 30 2468.0 31 2992.0 32 3997.0 33 5352.0 34 7216.0 35 11058.0 36 18507.0 37 22514.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.19700157509284 22.09038963427954 11.838489750399523 30.8741190402281 2 17.163667914878072 22.00429262946388 36.043245958535024 24.788793497123024 3 17.088318567905745 24.962325326513838 28.076765001369985 29.87259110421043 4 13.213535482692485 18.04845191341675 39.04009498584345 29.69791761804731 5 12.68038177002466 36.08777057265504 36.05922915334734 15.172618503972965 6 26.90314183943739 36.89263859713216 19.61937163211252 16.584847931317928 7 27.090373550095897 29.363412183761074 23.53068773403964 20.01552653210339 8 22.383322677870122 34.50315097269157 21.90839346059001 21.205132888848297 9 22.84683532742716 17.396565896428896 22.96100100465796 36.79559777148598 10-11 24.757397935884555 25.28769750662161 27.73369714129144 22.221207416202393 12-13 23.668828203488903 23.803543702621244 26.40994611380035 26.117681980089507 14-15 21.330715133802176 25.272855968581602 24.390926111973695 29.005502785642523 16-17 21.930655767650016 27.821604712759157 26.326605169421864 23.921134350168966 18-19 21.355260754406796 27.281601059457483 26.828363320851217 24.5347748652845 20-21 20.925426979632846 27.642935427892958 26.75015983194812 24.681477760526075 22-23 22.809160653941 26.735318293908115 26.963649648369714 23.49187140378117 24-25 21.770823819526896 27.953466069960726 26.566353091606537 23.709357018905834 26-27 20.41796054434195 28.124143757420768 27.78678418120376 23.67111151703352 28-29 22.04196730295004 26.947095625171247 28.620193624988584 22.390743446890127 30-31 21.771965476299204 27.12747739519591 27.555027856425244 23.545529272079644 32-33 20.516143026760435 28.137843638688466 27.46141200109599 23.88460133345511 34-35 21.588729564343776 27.447712119828292 27.56016531190063 23.4033930039273 36-37 22.392455932048588 26.873458763357384 27.06354461594666 23.670540688647364 38-39 21.640674947483788 27.71885560325144 27.749680336103754 22.89078911316102 40-41 21.591012877888392 26.569778061923465 28.07448168782537 23.764727372362774 42-43 21.10124212256827 25.99609553383871 28.730363503516305 24.17229884007672 44-45 20.60119645629738 26.058886656315643 28.700680427436296 24.63923645995068 46-47 20.672550004566627 25.574824184857064 29.308041830304138 24.44458398027217 48-49 19.713558315827928 25.934446068134076 30.425723810393645 23.92627180564435 50-51 19.466960453009406 26.216435290894147 29.671088683898073 24.645515572198374 52-53 19.15186318385241 26.185039729655674 29.76185039729656 24.90124668919536 54-55 18.619280299570736 26.91570006393278 29.309183487076446 25.15583614942004 56-57 18.602726276372273 26.723330897798885 29.278929582610285 25.395013243218557 58-59 18.787674673486162 26.272947301123388 28.960978171522513 25.978399853867934 60-61 19.000022833135446 26.437345876335737 28.96554479861175 25.59708649191707 62-63 19.035414193076996 25.9093296191433 28.6795597771486 26.375696410631104 64-65 20.066330258471094 25.865375833409445 27.68746004201297 26.380833866106496 66-67 19.923052333546444 26.381975522878804 26.82151338021737 26.873458763357384 68-69 19.712987487441776 25.747214357475567 26.810667640880446 27.72913051420221 70-71 20.389989953420404 26.25582244953877 26.35571741711572 26.998470179925103 72-73 20.583500776326606 26.746164033245044 25.544570280390904 27.125764910037446 74-75 19.93675221481414 28.000844826011505 25.17467348616312 26.887729473011234 76 20.06119280299571 27.594985843456023 25.51146223399397 26.8323591195543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 2.5 15 4.5 16 4.0 17 3.0 18 6.0 19 10.5 20 20.0 21 28.0 22 33.0 23 57.0 24 87.5 25 99.0 26 161.5 27 265.5 28 368.0 29 429.0 30 553.0 31 736.5 32 1001.5 33 1207.0 34 1386.0 35 1905.5 36 2443.0 37 2640.0 38 3000.0 39 3818.5 40 4702.5 41 5413.0 42 5698.0 43 5860.5 44 6261.5 45 6666.0 46 6832.0 47 6813.0 48 6508.0 49 5852.5 50 5483.0 51 5121.5 52 4274.0 53 3435.5 54 3083.0 55 2894.0 56 2297.5 57 1631.0 58 1372.0 59 1178.0 60 861.0 61 619.0 62 500.0 63 400.0 64 282.5 65 212.5 66 127.5 67 95.0 68 82.5 69 62.5 70 45.0 71 35.0 72 29.5 73 19.5 74 12.5 75 10.0 76 10.5 77 9.5 78 8.5 79 9.0 80 6.5 81 4.0 82 3.5 83 3.0 84 2.5 85 2.5 86 2.5 87 2.0 88 2.0 89 1.0 90 0.0 91 0.5 92 1.0 93 1.0 94 1.0 95 1.5 96 2.0 97 1.0 98 1.0 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6998356014247876 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 87592.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.258288428166956 #Duplication Level Percentage of deduplicated Percentage of total 1 72.67952840300107 38.707872865101834 2 10.842443729903536 11.548999908667458 3 5.717041800643087 9.134395835236095 4 3.2090032154340835 6.836240752580144 5 2.064308681672026 5.497077358662891 6 1.4040728831725615 4.486711115170335 7 0.9260450160771705 3.4523700794593113 8 0.6945337620578778 2.959174353822267 9 0.5466237942122186 2.620102292446799 >10 1.9121114683815648 14.632614850671294 >50 0.004287245444801715 0.12444058818156908 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00342497031692392 0.0 2 0.0 0.0 0.0 0.00342497031692392 0.0 3 0.0 0.0 0.0 0.00342497031692392 0.0 4 0.0 0.0 0.0 0.007991597406155813 0.0 5 0.0 0.0 0.0 0.007991597406155813 0.0 6 0.0 0.0 0.0 0.007991597406155813 0.0 7 0.0 0.0 0.0 0.007991597406155813 0.0 8 0.0 0.0 0.0 0.007991597406155813 0.0 9 0.0 0.0 0.0 0.0171248515846196 0.0 10 0.0 0.0 0.0 0.02054982190154352 0.0 11 0.0 0.0 0.0 0.026258105763083385 0.0 12 0.0 0.0 0.0 0.02739976253539136 0.0 13 0.0 0.0 0.0 0.029683076080007306 0.0 14 0.0 0.0 0.0 0.04109964380308704 0.0 15 0.0 0.0 0.0 0.043382957347702984 0.0 16 0.0 0.0 0.0 0.04909124120924285 0.0 17 0.0 0.0 0.0 0.05479952507078272 0.0 18 0.0 0.0 0.0 0.05822449538770664 0.0 19 0.0 0.0 0.0 0.06393277924924651 0.0 20 0.0 0.0 0.0 0.06964106311078637 0.0 21 0.0 0.0 0.0 0.07078271988309434 0.0 22 0.0 0.0 0.0 0.07649100374463422 0.0 23 0.0 0.0 0.0 0.07763266051694219 0.0 24 0.0 0.0 0.0 0.085624257923098 0.0 25 0.0 0.0 0.0 0.09589916887386976 0.0 26 0.0 0.0 0.0 0.10046579596310165 0.0 27 0.0 0.0 0.0 0.13014887204310896 0.0 28 0.0 0.0 0.0 0.15754863457850032 0.0 29 0.0 0.0 0.0 0.1883733674308156 0.0 30 0.0 0.0 0.0 0.22490638414467073 0.0 31 0.0 0.0 0.0 0.27970590921545346 0.0 32 0.0 0.0 0.0 0.3253721801077724 0.0 33 0.0 0.0 0.0 0.3733217645447073 0.0 34 0.0 0.0 0.0 0.426979632843182 0.0 35 0.0 0.0 0.0 0.5320120558955156 0.0 36 0.0 0.0 0.0 0.6884190337017079 0.0 37 0.0 0.0 0.0 1.0057996164033245 0.0 38 0.0 0.0 0.0 1.414512740889579 0.0 39 0.0 0.0 0.0 1.8997168691204676 0.0 40 0.0 0.0 0.0 2.4968033610375375 0.0 41 0.0 0.0 0.0 3.5311443967485614 0.0 42 0.0 0.0 0.0 4.678509452918075 0.0 43 0.0 0.0 0.0 5.812174627819892 0.0 44 0.0 0.0 0.0 6.694675312813955 0.0 45 0.0 0.0 0.0 7.730158005297287 0.0 46 0.0 0.0 0.0 8.743949219106767 0.0 47 0.0 0.0 0.0 9.764590373550096 0.0 48 0.0 0.0 0.0 10.599141474107224 0.0 49 0.0 0.0 0.0 11.391451274088958 0.0 50 0.0 0.0 0.0 12.168919536030687 0.0 51 0.0 0.0 0.0 13.251210156178647 0.0 52 0.0 0.0 0.0 14.255868115809664 0.0 53 0.0 0.0 0.0 15.40094985843456 0.0 54 0.0 0.0 0.0 16.378208055530184 0.0 55 0.0 0.0 0.0 17.250433829573478 0.0 56 0.0 0.0 0.0 18.03018540505982 0.0 57 0.0 0.0 0.0 18.711754498127682 0.0 58 0.0 0.0 0.0 19.449264773038635 0.0 59 0.0 0.0 0.0 20.179925107315736 0.0 60 0.0 0.0 0.0 20.812402959174353 0.0 61 0.0 0.0 0.0 21.465430632934513 0.0 62 0.0 0.0 0.0 22.260023746460863 0.0 63 0.0 0.0 0.0 23.157365969494933 0.0 64 0.0 0.0 0.0 23.95766736688282 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGC 20 7.891029E-5 69.99429 2 CCAAGTC 15 0.002218745 69.994286 62 AAGGCCA 15 0.002218745 69.994286 25 GTACATG 25 2.3132998E-4 56.31706 1 GCGAACT 25 2.3797738E-4 55.99543 28 TGGGTCG 25 2.3797738E-4 55.99543 14 ACCAGTC 25 2.3797738E-4 55.99543 18 TAAGACT 25 2.3797738E-4 55.99543 9 AAAGCGA 25 2.3797738E-4 55.99543 25 CGTTGCA 20 0.0069329483 52.49572 53 ATTGTCT 20 0.0069329483 52.49572 8 TACTGTA 20 0.0069329483 52.49572 11 CAAGGCC 20 0.0069329483 52.49572 24 CCAAGGC 20 0.0069329483 52.49572 23 ACCAAGG 20 0.0069329483 52.49572 22 GCGTCAT 20 0.0069329483 52.49572 3 CGTCATA 20 0.0069329483 52.49572 4 CCGTTGC 20 0.0069329483 52.49572 52 AGACTTC 30 5.851882E-4 46.662857 11 GGGTCGC 30 5.851882E-4 46.662857 15 >>END_MODULE