FastQCFastQC Report
Wed 25 May 2016
SRR1780596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87592
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT8360.9544250616494656TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT4050.4623709927847292TruSeq Adapter, Index 5 (96% over 26bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1250.14270709653849667No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC940.10731573659694948RNA PCR Primer, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACC150.00221802770.024
CTAAAAG150.00221802770.013
TGCTCAG150.00221802770.055
TTTCGCC308.3158775E-658.33333229
CACTAAG308.3158775E-658.33333236
TCCAATA308.3158775E-658.33333250
AGACTTC252.3788145E-455.99999611
AAGACTT252.3788145E-455.99999610
TATCACG200.006930711752.520
AAGAGCA200.006930711752.517
AGGAGCC200.006930711752.57
CTCTTGC200.006930711752.551
ATACTGT200.006930711752.551
TAATTCA200.006930711752.568
TATAGGT200.006930711752.539
ATCGATA200.006930711752.523
GGGATCA200.006930711752.529
AATCGAT200.006930711752.522
TATCCAG200.006930711752.55
TAATCGA200.006930711752.521
Produced by FastQC (version 0.11.5)