FastQCFastQC Report
Wed 25 May 2016
SRR1780595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911698
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAT207.90296E-443.946352
CAGTCGT207.909367E-443.939129
GCAACGC250.002335566635.25183518
CATACGA250.00235263335.1995621
CCAGTCG250.00236852335.1512958
CTCAATG502.3862394E-630.7573837
ATGCTCC300.00574462929.3329681
ACGTCGT300.005755430629.32169528
TCGGACG300.0057832829.2927478
TACGATC300.0057832829.2927473
CGTGCGA703.5775884E-828.29468310
ATTAGAG1500.027.828113
GGGATAC407.0879224E-427.4619525
CGAGAAT756.938717E-826.42724814
GTACAAG2000.026.399671
CATCGTT858.289135E-925.86498532
TAACGGC1450.025.80979236
TCGTTCG1200.025.67480523
GGTATAC609.854839E-625.6311533
CGCCAAC701.119477E-625.17434315
Produced by FastQC (version 0.11.5)