FastQCFastQC Report
Wed 25 May 2016
SRR1780595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911698
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT129691.4225105243183598No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT56920.6243295477230398RNA PCR Primer, Index 19 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC18570.2036858696629805No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC14580.159921377473681No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG12750.13984894120640826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAT207.854563E-444.0012054
CGTGCAT207.8566844E-443.99879516
GAGGTAC207.8566844E-443.99879537
CCGTCTT6350.037.07391444
GACACGC301.3006742E-436.667672
ACGCATG301.301094E-436.665665
TTCCTCG250.002351540635.20289641
CGTGCGA1000.035.20096610
GTGCATC250.002353439835.19710517
CGAACGC250.002353439835.19710526
GCCGTCT6900.034.11874843
CGGTAAG1250.031.67739128
CCGTGCG1050.031.4277089
CCCGTGC1100.029.9975328
GTCACTA300.005741972529.33574742
CAACGCA300.00574351229.33413714
AACGCAC300.00574351229.33413715
GTTCACG300.00574351229.33413711
TCGTTCG1350.029.3309223
GGAGACG300.005746592329.330927
Produced by FastQC (version 0.11.5)