FastQCFastQC Report
Wed 25 May 2016
SRR1780594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65013
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCAC150.002216123369.9946164
CGATGGG150.002216123369.9946158
CTTGTAC252.3751383E-455.9956933
TGCCTAC252.3751383E-455.99569345
CCTTGTA252.3751383E-455.9956932
GGCACCC200.006924820652.4959618
TATGCTG200.006924820652.4959632
CCCAAGT200.006924820652.4959662
TCGCTCA200.006924820652.4959669
CTTGAGA200.006924820652.4959648
GGTATGC200.006924820652.4959630
AGACATC200.006924820652.4959621
TTTTGCT200.006924820652.495962
GAGGCAT200.006924820652.4959619
TCCTTGT305.6869345E-446.9157031
TACGACA305.8405503E-446.66307449
GCCTACG305.8405503E-446.66307446
AGCTCCA305.8405503E-446.66307436
GAGGGAT305.8405503E-446.66307466
GCTCCAT305.8405503E-446.66307437
Produced by FastQC (version 0.11.5)