##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780594_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65013 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.237521726423946 34.0 31.0 34.0 12.0 34.0 2 31.620260563271962 34.0 33.0 34.0 23.0 34.0 3 31.830741543998894 34.0 34.0 34.0 23.0 34.0 4 32.04792887576331 34.0 34.0 34.0 27.0 34.0 5 31.935274483564825 34.0 34.0 34.0 27.0 34.0 6 35.0604648301109 38.0 37.0 38.0 27.0 38.0 7 34.40739544398812 38.0 35.0 38.0 24.0 38.0 8 33.610877824435114 37.0 34.0 38.0 21.0 38.0 9 34.13217356528694 37.0 34.0 38.0 24.0 38.0 10-11 34.49796963684186 38.0 35.5 38.0 25.0 38.0 12-13 34.76416255210496 38.0 36.0 38.0 26.0 38.0 14-15 34.46752957100888 38.0 35.5 38.0 25.0 38.0 16-17 34.31007644624921 38.0 35.0 38.0 24.0 38.0 18-19 34.33544060418686 38.0 35.0 38.0 24.0 38.0 20-21 34.42039284450802 38.0 35.0 38.0 24.0 38.0 22-23 34.2964560933967 38.0 35.0 38.0 23.5 38.0 24-25 34.717964099487794 38.0 36.0 38.0 25.5 38.0 26-27 34.70325934813037 38.0 36.0 38.0 25.5 38.0 28-29 34.86566532847277 38.0 36.0 38.0 26.0 38.0 30-31 34.77441434789965 38.0 36.0 38.0 26.0 38.0 32-33 34.370810453293956 38.0 35.5 38.0 24.0 38.0 34-35 34.437743220586654 38.0 35.0 38.0 24.0 38.0 36-37 34.45574731207604 38.0 35.5 38.0 24.5 38.0 38-39 34.858751326657746 38.0 36.0 38.0 26.0 38.0 40-41 34.405426606986296 38.0 35.5 38.0 24.0 38.0 42-43 34.70470521280359 38.0 36.0 38.0 26.0 38.0 44-45 33.81272976173996 37.0 34.0 38.0 22.5 38.0 46-47 34.51867318843924 37.5 35.5 38.0 25.0 38.0 48-49 34.33111070093673 38.0 35.0 38.0 24.0 38.0 50-51 34.17319613000477 37.5 34.5 38.0 23.5 38.0 52-53 34.1945918508606 37.0 34.5 38.0 23.5 38.0 54-55 33.98361866088321 37.0 34.0 38.0 23.0 38.0 56-57 34.00332241244059 37.0 34.5 38.0 23.5 38.0 58-59 33.95937735529817 37.0 34.5 38.0 23.0 38.0 60-61 33.962222940027374 37.0 34.0 38.0 23.5 38.0 62-63 34.0203882300463 37.0 34.0 38.0 23.5 38.0 64-65 34.09661144694138 37.0 34.5 38.0 24.0 38.0 66-67 34.01971144232692 37.0 34.0 38.0 23.0 38.0 68-69 33.485633642502265 37.0 33.5 38.0 21.0 38.0 70-71 33.53976896928307 37.0 34.0 38.0 22.0 38.0 72-73 33.67161183147986 37.0 34.0 38.0 22.5 38.0 74-75 33.22705458908219 37.0 32.5 38.0 21.0 38.0 76 32.52870964268685 37.0 31.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.427968651919503 1101 2 -1.3260160394530907 1101 3 -0.35227870813568885 1101 4 -3.2220494426444652 1101 5 -1.031605986023969 1101 6 -3.078810679018744 1101 7 -1.6923928071472858 1101 8 -0.8675030006861206 1101 9 -1.1276193285915497 1101 10-11 -1.9251525047818703 1101 12-13 -2.0250146114288654 1101 14-15 -0.6842194333905525 1101 16-17 -2.998665497046545 1101 18-19 -0.5506436000080797 1101 20-21 -1.22311724652846 1101 22-23 -0.058075207975441856 1101 24-25 -1.1376906215315472 1101 26-27 -2.7863759703646735 1101 28-29 -0.714614253519521 1101 30-31 -1.8133226414096413 1101 32-33 -0.14332217159545024 1101 34-35 -1.7118868112500678 1101 36-37 -0.1992629972807194 1101 38-39 -0.761234460779022 1101 40-41 -0.456412342124743 1101 42-43 -1.4804045820935485 1101 44-45 -0.12729519254174448 1101 46-47 -0.10490846724547964 1101 48-49 -0.588480477903083 1101 50-51 -1.2885120708035132 1101 52-53 -0.7080233990759837 1101 54-55 -1.7744013554069866 1101 56-57 -1.3273973143325293 1101 58-59 -1.1498983817987636 1101 60-61 -0.32431651096702296 1101 62-63 -1.8558226211707165 1101 64-65 -0.6338183015172163 1101 66-67 -1.635178484625861 1101 68-69 -0.05008288875364286 1101 70-71 -0.3282504531624326 1101 72-73 -1.1440476519980436 1101 74-75 -2.4104830501399235 1101 76 -2.0472735624796243 1102 1 0.5802827142007132 1102 2 -0.5051690449175723 1102 3 -0.0985082163324087 1102 4 -0.34661228417451895 1102 5 0.0936125932110059 1102 6 -0.4092751598930633 1102 7 1.0464050070603648 1102 8 1.3082620266362852 1102 9 0.3483096331570863 1102 10-11 -0.6191005922135702 1102 12-13 -4.20963209776766 1102 14-15 -1.7240145153577657 1102 16-17 -2.795427792128514 1102 18-19 -1.215138135527198 1102 20-21 -0.6735544049984057 1102 22-23 -0.699099798139379 1102 24-25 -0.4708190368320899 1102 26-27 -6.420105478561393 1102 28-29 -0.8344229966889216 1102 30-31 -0.8537324774752122 1102 32-33 -7.887639111486163 1102 34-35 -1.241804844036956 1102 36-37 -0.3967903196850955 1102 38-39 -0.25820167389377247 1102 40-41 -0.42885769731599765 1102 42-43 -0.40048654930666316 1102 44-45 -6.610642187077261 1102 46-47 -1.031834696753684 1102 48-49 -1.2130433194331403 1102 50-51 -7.377583109054882 1102 52-53 -2.5813840548136895 1102 54-55 0.10335820743454605 1102 56-57 -2.632670538376246 1102 58-59 -0.22939291731789524 1102 60-61 -0.3354094071418814 1102 62-63 -5.4303308178920275 1102 64-65 -1.5471926184571032 1102 66-67 -0.4741538944619279 1102 68-69 -7.999550101868394 1102 70-71 -1.0687559176433012 1102 72-73 -0.6738154115608879 1102 74-75 -6.919854634839378 1102 76 -2.5972189176708795 1103 1 -0.19156060377163442 1103 2 -0.17698932141527024 1103 3 0.055593166156072016 1103 4 0.02760430568723038 1103 5 -0.26161782153554114 1103 6 -1.5036299986027402 1103 7 0.6003220577516117 1103 8 0.9772251602768378 1103 9 0.15519903407874125 1103 10-11 0.609044569076751 1103 12-13 -1.2224661991501549 1103 14-15 -0.5409845614407089 1103 16-17 -0.7254162713911896 1103 18-19 -0.5865782276930958 1103 20-21 -0.346042884261081 1103 22-23 -0.5300099363882254 1103 24-25 -0.1549780230072102 1103 26-27 -0.7172137734922686 1103 28-29 0.11062308626038941 1103 30-31 -0.2610827078899618 1103 32-33 -4.665104549274183 1103 34-35 -1.3707218947281987 1103 36-37 -0.031110596182791994 1103 38-39 -0.15195743426244235 1103 40-41 0.19576211835219937 1103 42-43 0.07013557050900232 1103 44-45 -5.009040804588782 1103 46-47 -0.37343607924215405 1103 48-49 -0.27431060053913114 1103 50-51 -0.6116844915433646 1103 52-53 -0.32942552946806813 1103 54-55 -0.4997293501691402 1103 56-57 0.19744466899702218 1103 58-59 -0.5867546684699647 1103 60-61 0.484728841706044 1103 62-63 0.2188166475457578 1103 64-65 0.3300078423723889 1103 66-67 -0.5595571202683729 1103 68-69 -3.892418765462864 1103 70-71 0.039481409545636836 1103 72-73 0.750389657563538 1103 74-75 -2.1166979528117267 1103 76 -0.6554677655971481 1104 1 -1.682293705013258 1104 2 -0.38309480910971416 1104 3 -0.333093369170836 1104 4 -0.24362101779897571 1104 5 -0.35243544172349317 1104 6 -0.98383847867035 1104 7 0.5590687625188835 1104 8 1.273786044103531 1104 9 -0.14289124020536548 1104 10-11 -0.08513771011750038 1104 12-13 -0.4041517484226844 1104 14-15 -0.34389442802151393 1104 16-17 -0.5328841240062374 1104 18-19 -2.582228634508965E-4 1104 20-21 0.6543495251326021 1104 22-23 0.3603871014239388 1104 24-25 0.46100410727270713 1104 26-27 0.42494910659144836 1104 28-29 -0.33322212332647894 1104 30-31 -0.6882957962525111 1104 32-33 -0.8276827796084305 1104 34-35 0.05262747037352966 1104 36-37 -0.4337226340955809 1104 38-39 -0.7165641193086216 1104 40-41 0.695913045042083 1104 42-43 0.5192732760208472 1104 44-45 -3.7339718814004073 1104 46-47 -0.013133823391235921 1104 48-49 0.6978082089511375 1104 50-51 1.0368273712944642 1104 52-53 -0.7386984652940427 1104 54-55 -0.3407029279366327 1104 56-57 0.2777879769075966 1104 58-59 0.3502686547345064 1104 60-61 -0.3271561320327123 1104 62-63 -0.39755789212783554 1104 64-65 -0.26098082806409906 1104 66-67 -0.16717791192917986 1104 68-69 -3.841657088767956 1104 70-71 -1.0904262233201578 1104 72-73 -0.12821497487966838 1104 74-75 -1.8039376042716881 1104 76 -0.6787909700726296 1105 1 -0.2185461146281149 1105 2 -0.060619495368023024 1105 3 -0.2334631712873616 1105 4 0.32919852663629356 1105 5 0.5390630436614572 1105 6 0.41122033560243665 1105 7 1.1328914935468504 1105 8 0.6073160806903388 1105 9 0.27069702054446765 1105 10-11 0.8347057140927348 1105 12-13 -1.422740205875769 1105 14-15 0.14357557473232418 1105 16-17 0.9138289646282445 1105 18-19 0.5640735761845121 1105 20-21 0.09049964905564423 1105 22-23 0.19934614780656545 1105 24-25 0.6923566388435844 1105 26-27 0.27388100792509107 1105 28-29 -0.5215851588510816 1105 30-31 -0.03422797297070446 1105 32-33 0.6287572849102361 1105 34-35 0.5426546154225065 1105 36-37 0.6393132839185043 1105 38-39 0.6770685963765004 1105 40-41 -0.11104238200068295 1105 42-43 0.4227116488915357 1105 44-45 1.2244253804903025 1105 46-47 0.9223770149580375 1105 48-49 -0.014709986099802563 1105 50-51 0.07151599004421882 1105 52-53 -0.484311982741616 1105 54-55 -0.6958309817546464 1105 56-57 -0.004697565403269266 1105 58-59 0.08418816376318716 1105 60-61 0.4056716739391959 1105 62-63 0.7187232361620275 1105 64-65 0.3492713455068568 1105 66-67 0.711003763195734 1105 68-69 0.9670489972307053 1105 70-71 0.9556810192936354 1105 72-73 0.07264855240307355 1105 74-75 -0.6639537339384773 1105 76 -0.7244261248780894 1106 1 -0.6461275422095412 1106 2 0.16931813456960754 1106 3 -0.6503030881272807 1106 4 -0.10981741237964826 1106 5 0.03220233680074713 1106 6 0.054186378568481075 1106 7 0.6412768019321575 1106 8 0.758390231764487 1106 9 1.0730532229006755 1106 10-11 0.07198915137617945 1106 12-13 -0.014632097767666608 1106 14-15 0.2714342025909531 1106 16-17 0.8288670796663595 1106 18-19 0.31518237729331133 1106 20-21 -0.5686826101266149 1106 22-23 0.02263097109138812 1106 24-25 0.19232198880893492 1106 26-27 0.3591893932334784 1106 28-29 -0.6312178684837875 1106 30-31 -0.39129658003931667 1106 32-33 0.7746685808215332 1106 34-35 0.724220796988682 1106 36-37 0.5998122444174641 1106 38-39 0.87731114661905 1106 40-41 0.46082178986348765 1106 42-43 0.6860519122317967 1106 44-45 1.1312167872817156 1106 46-47 0.7170755596565783 1106 48-49 0.7340079626181435 1106 50-51 1.055750224278448 1106 52-53 -0.33715328558292157 1106 54-55 0.5292556433319859 1106 56-57 1.0274576667519568 1106 58-59 0.3825942621692846 1106 60-61 1.0438213620888845 1106 62-63 0.9223614898002808 1106 64-65 0.782743278978792 1106 66-67 0.9302691824611529 1106 68-69 0.9910268212085285 1106 70-71 0.4213081849207967 1106 72-73 0.7817615115160308 1106 74-75 -1.8712648912496306 1106 76 -0.8454240458760083 1107 1 0.48585875106707377 1107 2 0.29305676153403937 1107 3 0.49338118458924995 1107 4 0.3455766559176432 1107 5 0.36511028906353715 1107 6 0.4502870520885054 1107 7 0.5680639932354836 1107 8 0.7514187086639339 1107 9 -1.1305152516355434 1107 10-11 -0.8310475968218682 1107 12-13 -0.2247703466155926 1107 14-15 -0.1677126720397908 1107 16-17 0.6017726687009812 1107 18-19 1.206048500417495 1107 20-21 0.9143257793333959 1107 22-23 -0.9839269870794709 1107 24-25 -0.5029042902883205 1107 26-27 -0.10200640022038243 1107 28-29 -1.285690277794913 1107 30-31 -0.33711957424940664 1107 32-33 1.36945766270739 1107 34-35 0.6177573679446127 1107 36-37 -0.25922119526113363 1107 38-39 0.6406692938481626 1107 40-41 0.7856238695042705 1107 42-43 0.3420249714306607 1107 44-45 0.1269038958720472 1107 46-47 -0.24901211610851703 1107 48-49 0.05518248701847739 1107 50-51 0.7500666605303721 1107 52-53 1.1129707838959888 1107 54-55 0.009487239692610672 1107 56-57 0.4140345307481823 1107 58-59 0.7865172209309605 1107 60-61 -0.16734489101285277 1107 62-63 -0.023118836325210168 1107 64-65 0.31618295757976256 1107 66-67 0.9634843543860043 1107 68-69 0.6928346907583318 1107 70-71 -0.5158181296248685 1107 72-73 -0.042237070547066935 1107 74-75 0.13214997345555446 1107 76 -1.6139931112192691 1108 1 -0.7897172857992842 1108 2 -0.19112142586995517 1108 3 -0.5308891687133617 1108 4 -0.6149456175078534 1108 5 -0.5399588353604265 1108 6 0.030010554392653432 1108 7 0.7892621499175121 1108 8 1.238976312350566 1108 9 -0.09764274779529813 1108 10-11 0.023637503024524165 1108 12-13 0.15441552127995095 1108 14-15 0.432056913213664 1108 16-17 -0.5953087445094667 1108 18-19 0.007123769234702593 1108 20-21 0.005493214049209882 1108 22-23 -0.3156474171870016 1108 24-25 -0.16189046540352336 1108 26-27 -0.4864150023709186 1108 28-29 0.23343414616822145 1108 30-31 0.030315141572408777 1108 32-33 0.25855136470431717 1108 34-35 -0.1406143678464744 1108 36-37 -0.016671272066055565 1108 38-39 0.6687030880109859 1108 40-41 0.25459468363637683 1108 42-43 0.2316563078361895 1108 44-45 0.7163816224465478 1108 46-47 0.5797129222939432 1108 48-49 0.056718585328766835 1108 50-51 -0.35853549000726304 1108 52-53 -0.41828881671845153 1108 54-55 0.20764392172026191 1108 56-57 0.9080437473380414 1108 58-59 -1.1802262506512307 1108 60-61 0.4912572595247866 1108 62-63 -0.06390224646345644 1108 64-65 -0.20040690417139473 1108 66-67 -0.8490348468428763 1108 68-69 1.0155689457506512 1108 70-71 1.111601225213839 1108 72-73 0.1801131598676804 1108 74-75 0.7160977461130074 1108 76 0.8637334632814984 1109 1 0.46281727641269654 1109 2 0.0718586048520109 1109 3 0.6131968527920186 1109 4 -0.0415201182759013 1109 5 0.24068632592990014 1109 6 0.8696418907981851 1109 7 -0.5471433800364096 1109 8 0.14773207271922928 1109 9 0.6114202322354245 1109 10-11 1.1966021727633915 1109 12-13 0.6116351833383291 1109 14-15 -0.1700168195052214 1109 16-17 -0.7807158105616949 1109 18-19 -0.08888237515853348 1109 20-21 0.6954317701168051 1109 22-23 1.1105430590034828 1109 24-25 0.6837316544121492 1109 26-27 0.017809267982386245 1109 28-29 -0.11748751281795222 1109 30-31 0.25274217690266454 1109 32-33 0.15747609588710532 1109 34-35 0.16844861218424256 1109 36-37 0.21543318261904432 1109 38-39 -0.006796143939858723 1109 40-41 -0.25354663740816363 1109 42-43 -0.05198424515920408 1109 44-45 -0.5574279013629777 1109 46-47 -0.5715927612698053 1109 48-49 -0.4540341028432735 1109 50-51 0.25697910292668524 1109 52-53 0.5461505073982948 1109 54-55 0.23298954384007686 1109 56-57 0.32647692706154174 1109 58-59 0.2934296633272737 1109 60-61 0.47781639930973085 1109 62-63 -0.8664368086754379 1109 64-65 -0.7068585170746147 1109 66-67 -0.40287509261860777 1109 68-69 0.4946780087306806 1109 70-71 0.42888233120462615 1109 72-73 -0.6090573470447609 1109 74-75 0.8602605725338961 1109 76 -0.32367053057410544 1110 1 0.31708899692322845 1110 2 0.4474225781190775 1110 3 0.3419106318351304 1110 4 0.10739295142757399 1110 5 0.10955500158796383 1110 6 0.7324787668085122 1110 7 1.2066144311441391 1110 8 1.8202777334425662 1110 9 -0.7763762307172613 1110 10-11 -0.05013462362718002 1110 12-13 0.7403417242218637 1110 14-15 0.32835459458988225 1110 16-17 0.33246487802855285 1110 18-19 0.631157675991119 1110 20-21 0.4160267468340564 1110 22-23 0.6643949662585271 1110 24-25 0.1724401254715744 1110 26-27 -0.6393070492420279 1110 28-29 0.1405770033110798 1110 30-31 0.33692197278656266 1110 32-33 1.079219527257294 1110 34-35 0.488954318266714 1110 36-37 0.1953955441892461 1110 38-39 0.586117697833977 1110 40-41 0.3851606272913344 1110 42-43 0.7140422465048601 1110 44-45 1.5285855616138377 1110 46-47 0.6700893870159561 1110 48-49 1.107454062765818 1110 50-51 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2116 46-47 0.054161238205672646 2116 48-49 0.261712778127837 2116 50-51 -0.7003473366971562 2116 52-53 0.6123151321781819 2116 54-55 -0.41314585760610356 2116 56-57 0.16867092503839132 2116 58-59 0.12912334284470717 2116 60-61 -1.1164663177109873 2116 62-63 -0.1788267528513785 2116 64-65 0.23490087747784827 2116 66-67 0.024890820985227435 2116 68-69 0.2703889225218532 2116 70-71 -2.985320958293709 2116 72-73 -0.259201590422677 2116 74-75 -0.8839603258962896 2116 76 -0.9725034705164113 2117 1 0.10151078437615269 2117 2 0.4424625340297972 2117 3 0.37938652050969424 2117 4 0.6236508737202193 2117 5 -1.0868260032802262 2117 6 -0.7509418265597247 2117 7 -2.51148972978174 2117 8 -2.402878324240909 2117 9 -0.12546229666747877 2117 10-11 -0.41019708344163774 2117 12-13 0.7072977267937404 2117 14-15 -0.3587952171121458 2117 16-17 0.35444062892411665 2117 18-19 0.08043203991139336 2117 20-21 0.13293682307177335 2117 22-23 -0.03607348234990582 2117 24-25 -0.3692839491127913 2117 26-27 0.9014296091579084 2117 28-29 1.008998055942655 2117 30-31 0.09284646989321033 2117 32-33 0.7718345727243587 2117 34-35 -0.272068001931693 2117 36-37 -1.3590271617903582 2117 38-39 -0.0010964107358759634 2117 40-41 -0.6851296271405616 2117 42-43 -1.1658374264996496 2117 44-45 -0.0010369239193721569 2117 46-47 -0.24547504763086891 2117 48-49 -0.5585696352226179 2117 50-51 1.0776800488398592 2117 52-53 0.8778264715020967 2117 54-55 -0.5066417925654534 2117 56-57 0.0618031458342827 2117 58-59 -0.19864730328280444 2117 60-61 0.6491519963668893 2117 62-63 -0.015155379295535454 2117 64-65 -0.16732419740447568 2117 66-67 -1.2010398593742053 2117 68-69 -0.46538343520172987 2117 70-71 0.4178668893742383 2117 72-73 1.1174565182636726 2117 74-75 0.3912857160378138 2117 76 0.6055880998729819 2118 1 0.7961234221653157 2118 2 0.690715910834637 2118 3 -0.04399494199612519 2118 4 0.770600105206011 2118 5 0.04436480560038447 2118 6 0.8449283799299465 2118 7 0.39861739644089766 2118 8 -3.7210742565495565 2118 9 -0.42642488011725277 2118 10-11 0.2407887883174027 2118 12-13 1.0216510880730425 2118 14-15 -0.49076230296837764 2118 16-17 -0.15266230540284909 2118 18-19 -0.09675317977499276 2118 20-21 -1.4901030775647754 2118 22-23 -0.7112015680508819 2118 24-25 -0.08730133771705084 2118 26-27 0.5971290347129425 2118 28-29 0.17155045463851337 2118 30-31 -0.33740504384689274 2118 32-33 0.7876706230271111 2118 34-35 1.0920447134851727 2118 36-37 -0.18552925773819595 2118 38-39 -0.2591308774335914 2118 40-41 -0.9130833610328111 2118 42-43 -0.5586723900146282 2118 44-45 -0.3431421870772624 2118 46-47 0.2703998165206585 2118 48-49 -0.4675123459818096 2118 50-51 0.3507354750159166 2118 52-53 -0.3152336123358168 2118 54-55 0.9225617472575536 2118 56-57 1.177196718260923 2118 58-59 1.4898327463989176 2118 60-61 -0.09797577882329733 2118 62-63 0.6314833414000063 2118 64-65 0.2304401249057264 2118 66-67 -2.043999027205288 2118 68-69 1.2263569777776198 2118 70-71 -0.15824706808577815 2118 72-73 -0.9333508097909728 2118 74-75 0.9373577545411536 2118 76 0.9606571885238111 2119 1 0.34654645046445154 2119 2 0.5436771089285806 2119 3 1.1006126627884676 2119 4 0.3590470564848225 2119 5 0.5831180877164996 2119 6 1.0915490159311148 2119 7 0.1738775345328918 2119 8 0.7290862024604579 2119 9 -0.5232837734363258 2119 10-11 0.5613664407534671 2119 12-13 0.46009317695760643 2119 14-15 0.4930459242026757 2119 16-17 0.48124803204731137 2119 18-19 0.3551091172200529 2119 20-21 0.42106097961698197 2119 22-23 0.7588947073551253 2119 24-25 0.28316447965142544 2119 26-27 0.6720099060539937 2119 28-29 0.7339835967176711 2119 30-31 1.2057730170302818 2119 32-33 1.4274158335687872 2119 34-35 1.2040742768421708 2119 36-37 0.15109429569951516 2119 38-39 0.8436115129194093 2119 40-41 0.8099060389477373 2119 42-43 0.44429367047355584 2119 44-45 0.7172973733622996 2119 46-47 1.015610358191374 2119 48-49 0.27833030694048233 2119 50-51 1.4037355722637983 2119 52-53 -0.08660383503347191 2119 54-55 -0.374773660697322 2119 56-57 1.1981536374479305 2119 58-59 1.3602499398249677 2119 60-61 0.8767883950559181 2119 62-63 1.1069768744156647 2119 64-65 1.3138788101143248 2119 66-67 1.02587357806555 2119 68-69 0.32399385417556203 2119 70-71 0.35390891752152953 2119 72-73 -0.5698868401323196 2119 74-75 1.2126178926331477 2119 76 -0.13260353305549444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 8.0 13 73.0 14 213.0 15 420.0 16 635.0 17 652.0 18 681.0 19 621.0 20 558.0 21 428.0 22 384.0 23 378.0 24 397.0 25 406.0 26 539.0 27 726.0 28 887.0 29 1063.0 30 1346.0 31 1819.0 32 2245.0 33 3273.0 34 4799.0 35 7859.0 36 14431.0 37 20171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.256580067154594 23.128104352669936 13.862627075370975 28.752688504804492 2 18.43477458354483 20.42822204789811 37.18025625644102 23.95674711211604 3 16.995062525956346 26.697737375601804 28.998815621491087 27.308384476950764 4 12.97894267300386 18.50091520157507 37.59555781151462 30.92458431390645 5 14.126405488133143 35.87128728100534 34.814575546429175 15.187731684432345 6 26.888468460154126 39.05680402381062 17.690308092227706 16.364419423807547 7 26.008644424961165 31.242982172796207 22.950794456493316 19.79757894574931 8 22.980019380739236 36.34196237675542 19.291526310122592 21.386491932382754 9 22.764677833664038 18.45169427652931 22.310922430898437 36.472705458908216 10-11 24.64660913971052 26.040176580068604 26.294741051789643 23.018473228431237 12-13 23.16690508052236 24.14517096580684 26.300124590466524 26.387799363204284 14-15 21.382646547613554 25.644871025794842 23.88060849368588 29.091873932905727 16-17 21.660283327949795 28.106686355036686 26.11939150631412 24.1136388106994 18-19 21.028102071893315 27.490655715010842 26.814637072585484 24.66660514051036 20-21 21.009644225001153 27.493732022826205 26.419331518311722 25.07729223386092 22-23 22.475504899020198 26.76541614753972 27.01613523449156 23.742943718948517 24-25 21.592604556011874 28.042852967867965 26.693122913878764 23.671419562241397 26-27 20.569732207404673 28.123606048021166 27.40221186531924 23.90444987925492 28-29 21.766415947579716 27.17148877916724 28.299724670450527 22.762370602802516 30-31 21.503391629366433 27.646009259686526 27.299924630458523 23.550674480488517 32-33 20.26210142586867 28.28511220832757 27.430667712611324 24.02211865319244 34-35 21.424945780074754 27.779828649654686 27.529878639656687 23.265346930613877 36-37 22.210942426899237 27.462199867718763 26.533154907480043 23.793702797901958 38-39 21.252672542414594 27.496808330641564 27.877501422792367 23.37301770415148 40-41 21.283435620568195 26.398566440558042 27.799055573500688 24.51894236537308 42-43 20.712780520818914 26.611600756771725 28.132065894513403 24.543552827895958 44-45 20.422838509221233 26.2824358205282 28.210511743805082 25.084213926445482 46-47 20.806607909187395 26.00787534800732 28.731176841554767 24.454339901250517 48-49 19.89294448802547 26.33704028425084 29.39104486794949 24.3789703597742 50-51 19.926783873994435 26.623905988033165 28.881915924507407 24.567394213465 52-53 19.216156768646268 26.59852644855644 29.423346100010768 24.76197068278652 54-55 18.91314044883331 27.182255856521003 28.483534062418286 25.4210696322274 56-57 19.450725239567472 26.730807684616924 28.730407764600923 25.08805931121468 58-59 19.41611677664467 26.686970298248042 28.448925599495485 25.4479873256118 60-61 19.57377755218187 26.08401396643748 28.46123083075692 25.88097765062372 62-63 19.484564625536432 26.487779367203483 28.255887284081645 25.77176872317844 64-65 20.115207727685235 26.63467306538692 27.420669712211403 25.82944949471644 66-67 20.367464968544752 26.658514450955963 26.86155076676972 26.11246981372956 68-69 19.869872179410272 26.612369833725563 26.661590758771325 26.856167228092843 70-71 20.942734530017074 26.091704735975878 26.582375832525802 26.383184901481243 72-73 21.13731099933859 26.20091366342116 25.63948748711796 27.022287850122282 74-75 20.937350991340193 26.62775137280236 25.46029255687324 26.974605078984204 76 21.452632550412996 25.960961653823084 25.12728223586052 27.459123559903404 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 1.0 14 4.5 15 5.5 16 4.5 17 5.0 18 5.0 19 8.5 20 14.5 21 17.0 22 23.0 23 43.5 24 65.0 25 72.0 26 98.5 27 174.0 28 282.0 29 341.0 30 406.0 31 534.5 32 782.0 33 966.0 34 1106.0 35 1504.5 36 1822.0 37 1881.0 38 2218.5 39 2916.5 40 3588.5 41 3972.0 42 4044.0 43 4290.5 44 4660.0 45 4785.5 46 4788.0 47 4875.0 48 4689.5 49 4203.5 50 3990.0 51 3710.0 52 3106.5 53 2555.0 54 2327.0 55 2178.5 56 1728.0 57 1247.0 58 1068.0 59 913.0 60 672.0 61 499.5 62 413.0 63 361.5 64 255.0 65 197.0 66 154.5 67 115.0 68 104.5 69 73.0 70 45.5 71 39.0 72 32.5 73 21.0 74 15.5 75 15.0 76 12.0 77 6.5 78 4.0 79 4.0 80 5.0 81 5.0 82 2.0 83 0.0 84 0.5 85 2.0 86 2.5 87 2.0 88 2.5 89 2.5 90 2.0 91 2.5 92 3.0 93 1.5 94 0.5 95 0.5 96 0.0 97 0.5 98 1.0 99 3.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5937274083644809 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 65013.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.456800947502806 #Duplication Level Percentage of deduplicated Percentage of total 1 71.71506044514744 39.053727715995265 2 12.029714156592474 13.101994985618262 3 5.965427635295447 9.745743159060495 4 3.3499039656535987 7.297002138033931 5 1.9715286408315447 5.368157137803209 6 1.2879900576206078 4.208389091412487 7 0.923624449214778 3.520834294679526 8 0.6496441080103943 2.8302031901312046 9 0.47734719240763757 2.339532093581284 >10 1.6297593492260762 12.53441619368434 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003076307815360005 0.0 2 0.0 0.0 0.0 0.003076307815360005 0.0 3 0.0 0.0 0.0 0.003076307815360005 0.0 4 0.0 0.0 0.0 0.003076307815360005 0.0 5 0.0 0.0 0.0 0.007690769538400012 0.0 6 0.0 0.0 0.0 0.007690769538400012 0.0 7 0.0 0.0 0.0 0.009228923446080015 0.0 8 0.0 0.0 0.0 0.009228923446080015 0.0 9 0.0 0.0 0.0 0.015381539076800024 0.0 10 0.0 0.0 0.0 0.026148616430560042 0.0 11 0.0 0.0 0.0 0.03076307815360005 0.0 12 0.0 0.0 0.0 0.03537753987664006 0.0 13 0.0 0.0 0.0 0.03691569378432006 0.0 14 0.0 0.0 0.0 0.03999200159968006 0.0 15 0.0 0.0 0.0 0.04306830941504007 0.0 16 0.0 0.0 0.0 0.04306830941504007 0.0 17 0.0 0.0 0.0 0.04460646332272007 0.0 18 0.0 0.0 0.0 0.05075907895344008 0.0 19 0.0 0.0 0.0 0.058449848491840095 0.0 20 0.0 0.0 0.0 0.058449848491840095 0.0 21 0.0 0.0 0.0 0.0630643102148801 0.0 22 0.0 0.0 0.0 0.0676787719379201 0.0 23 0.0 0.0 0.0 0.06921692584560012 0.0 24 0.0 0.0 0.0 0.06921692584560012 0.0 25 0.0 0.0 0.0 0.07536954147632012 0.0 26 0.0 0.0 0.0 0.07690769538400012 0.0 27 0.0 0.0 0.0 0.09998000399920016 0.0 28 0.0 0.0 0.0 0.1245904665220802 0.0 29 0.0 0.0 0.0 0.14304831341424024 0.0 30 0.0 0.0 0.0 0.1845784689216003 0.0 31 0.0 0.0 0.0 0.21841785489056034 0.0 32 0.0 0.0 0.0 0.2445664713211204 0.0 33 0.0 0.0 0.0 0.2922492424592005 0.0 34 0.0 0.0 0.0 0.34300832141264054 0.0 35 0.0 0.0 0.0 0.40453447771984063 0.0 36 0.0 0.0 0.0 0.5429683294110409 0.0 37 0.0 0.0 0.0 0.8229123406088014 0.0 38 0.0 0.0 0.0 1.1197760447910419 0.0 39 0.0 0.0 0.0 1.5566117545721625 0.0 40 0.0 0.0 0.0 2.070355159737283 0.0 41 0.0 0.0 0.0 2.7209942626859243 0.0 42 0.0 0.0 0.0 3.5085290634180857 0.0 43 0.0 0.0 0.0 4.4037346376878475 0.0 44 0.0 0.0 0.0 5.191269438420008 0.0 45 0.0 0.0 0.0 5.988033162598249 0.0 46 0.0 0.0 0.0 6.707889191392491 0.0 47 0.0 0.0 0.0 7.530801532001292 0.0 48 0.0 0.0 0.0 8.406011105471213 0.0 49 0.0 0.0 0.0 9.128943442080814 0.0 50 0.0 0.0 0.0 9.754972082506576 0.0 51 0.0 0.0 0.0 10.536354267608017 0.0 52 0.0 0.0 0.0 11.374648147293618 0.0 53 0.0 0.0 0.0 12.17910264101026 0.0 54 0.0 0.0 0.0 12.91741651669666 0.0 55 0.0 0.0 0.0 13.740328857305462 0.0 56 0.0 0.0 0.0 14.443265193115224 0.0 57 0.0 0.0 0.0 15.133896297663544 0.0 58 0.0 0.0 0.0 15.821451094396505 0.0 59 0.0 0.0 0.0 16.442865273099226 0.0 60 0.0 0.0 0.0 17.024287450202266 0.0 61 0.0 0.0 0.0 17.645701628904988 0.0 62 0.0 0.0 0.0 18.31941304046883 0.0 63 0.0 0.0 0.0 19.01465860674019 0.0 64 0.0 0.0 0.0 19.78527371448787 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCCAC 15 0.0022161233 69.99461 64 CGATGGG 15 0.0022161233 69.99461 58 CTTGTAC 25 2.3751383E-4 55.995693 3 TGCCTAC 25 2.3751383E-4 55.995693 45 CCTTGTA 25 2.3751383E-4 55.995693 2 GGCACCC 20 0.0069248206 52.49596 18 TATGCTG 20 0.0069248206 52.49596 32 CCCAAGT 20 0.0069248206 52.49596 62 TCGCTCA 20 0.0069248206 52.49596 69 CTTGAGA 20 0.0069248206 52.49596 48 GGTATGC 20 0.0069248206 52.49596 30 AGACATC 20 0.0069248206 52.49596 21 TTTTGCT 20 0.0069248206 52.49596 2 GAGGCAT 20 0.0069248206 52.49596 19 TCCTTGT 30 5.6869345E-4 46.915703 1 TACGACA 30 5.8405503E-4 46.663074 49 GCCTACG 30 5.8405503E-4 46.663074 46 AGCTCCA 30 5.8405503E-4 46.663074 36 GAGGGAT 30 5.8405503E-4 46.663074 66 GCTCCAT 30 5.8405503E-4 46.663074 37 >>END_MODULE