FastQCFastQC Report
Wed 25 May 2016
SRR1780594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65013
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT5790.8905911125467214No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT5210.8013781859012813RNA PCR Primer, Index 48 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1170.1799640071985603No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC660.10151815790688015RNA PCR Primer, Index 48 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGG150.002215447470.04
TAACGAG150.002215447470.065
GGTATGA150.002215447470.041
TTAACGA150.002215447470.064
TTCGGTC150.002215447470.028
ATTGGCG150.002215447470.021
GGTTAAC150.002215447470.062
TCGGTCA150.002215447470.029
CGCCATT150.002215447470.017
GAACCCT252.3742356E-455.9999963
GGAATGA200.00692271352.50000440
GAGGACT200.00692271352.50000433
CTCGTGG200.00692271352.5000048
TATGGGT200.00692271352.5000046
GCTATAA200.00692271352.5000041
CGTCTTT200.00692271352.50000425
TACGGTG200.00692271352.50000455
TTCAAAT200.00692271352.50000428
CCTCGTG200.00692271352.5000047
CGACAAT200.00692271352.50000417
Produced by FastQC (version 0.11.5)