##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780594_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65013 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30630796917539 34.0 34.0 34.0 27.0 34.0 2 33.05891129466414 34.0 34.0 34.0 32.0 34.0 3 33.345346315352316 34.0 34.0 34.0 33.0 34.0 4 33.3794318059465 34.0 34.0 34.0 33.0 34.0 5 33.376601602756374 34.0 34.0 34.0 34.0 34.0 6 37.055881131466016 38.0 37.0 38.0 36.0 38.0 7 36.617922569332286 38.0 37.0 38.0 34.0 38.0 8 36.59908018396321 38.0 37.0 38.0 34.0 38.0 9 36.41871625674865 38.0 37.0 38.0 34.0 38.0 10-11 36.06780182425054 38.0 36.5 38.0 32.5 38.0 12-13 35.95520895820836 38.0 37.0 38.0 32.0 38.0 14-15 36.22196329964776 38.0 37.0 38.0 33.0 38.0 16-17 36.38326949994617 38.0 37.0 38.0 34.0 38.0 18-19 36.3832771907157 38.0 37.0 38.0 34.0 38.0 20-21 36.08330641563995 38.0 37.0 38.0 33.0 38.0 22-23 36.097903496223836 38.0 36.5 38.0 32.5 38.0 24-25 35.33561749188624 37.5 35.5 38.0 29.0 38.0 26-27 35.60515589189855 38.0 36.0 38.0 31.0 38.0 28-29 35.42323842923723 38.0 36.0 38.0 31.0 38.0 30-31 35.10262562872041 37.0 35.5 38.0 29.0 38.0 32-33 35.653576976912305 38.0 36.0 38.0 31.0 38.0 34-35 35.669489179087265 38.0 36.5 38.0 32.0 38.0 36-37 35.87530186270438 38.0 37.0 38.0 32.0 38.0 38-39 35.532962638241585 38.0 36.5 38.0 29.5 38.0 40-41 34.90895667020442 37.0 35.5 38.0 27.0 38.0 42-43 35.34789965083907 37.5 35.5 38.0 29.0 38.0 44-45 35.60496362266008 38.0 36.0 38.0 31.0 38.0 46-47 35.88779936320428 38.0 37.0 38.0 32.0 38.0 48-49 35.62550566809715 38.0 36.5 38.0 31.0 38.0 50-51 35.865726854629074 38.0 36.5 38.0 32.0 38.0 52-53 35.753118607047824 38.0 36.0 38.0 31.5 38.0 54-55 35.9487487117961 38.0 37.0 38.0 33.0 38.0 56-57 36.030224724285915 38.0 37.0 38.0 33.0 38.0 58-59 36.11133927060742 38.0 37.0 38.0 34.0 38.0 60-61 35.70890437297156 38.0 36.5 38.0 32.5 38.0 62-63 36.00118437850891 38.0 37.0 38.0 33.0 38.0 64-65 36.019519173088455 38.0 37.0 38.0 33.0 38.0 66-67 35.29428729638688 38.0 36.0 38.0 29.0 38.0 68-69 34.660860135665175 37.0 34.5 38.0 26.0 38.0 70-71 34.640095057911495 37.0 35.0 38.0 26.0 38.0 72-73 34.3163213511144 37.0 34.0 38.0 24.5 38.0 74-75 35.107586175072676 37.5 35.5 38.0 29.0 38.0 76 34.52644855644256 37.0 34.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7917131678468365 1101 2 0.8384400995548376 1101 3 0.40695915807457084 1101 4 0.6163717167256664 1101 5 0.49576685522801966 1101 6 -0.17841848518995818 1101 7 0.6426452174481341 1101 8 0.9022041712914586 1101 9 0.7392745092494053 1101 10-11 0.5622801518448242 1101 12-13 0.7961400591943288 1101 14-15 0.7309442361978427 1101 16-17 -0.035789072887489226 1101 18-19 -0.2558206415387758 1101 20-21 0.41052230287364466 1101 22-23 0.78847134654999 1101 24-25 -1.1661651986707966 1101 26-27 0.1863758917260938 1101 28-29 1.3493603101775662 1101 30-31 1.3414447074977645 1101 32-33 0.7515212478371893 1101 34-35 0.9505379205877986 1101 36-37 0.23056411802502907 1101 38-39 0.15532158010187658 1101 40-41 1.6420475940278507 1101 42-43 0.9508290929048471 1101 44-45 0.9088945583941168 1101 46-47 1.1812765651800845 1101 48-49 -1.3532123539484715 1101 50-51 -1.4494028683319158 1101 52-53 -1.3814878120962675 1101 54-55 -0.6486023005667079 1101 56-57 -1.4856186962025788 1101 58-59 -0.9549508957574275 1101 60-61 -0.8926680567515319 1101 62-63 -0.3271205276635172 1101 64-65 -0.934388729684045 1101 66-67 -1.3563149314565166 1101 68-69 -0.8613471413018203 1101 70-71 -0.9893617136209087 1101 72-73 -1.0570268854968177 1101 74-75 -0.13580994733552387 1101 76 -0.439640759861085 1102 1 -0.4479316408963321 1102 2 0.4419100449100313 1102 3 0.36518320179042263 1102 4 0.5977651593486186 1102 5 0.13737887708594343 1102 6 0.16819353666796388 1102 7 -0.37710888091252315 1102 8 0.6691167395974702 1102 9 0.883126968265799 1102 10-11 0.7673484578557535 1102 12-13 0.4545826821451442 1102 14-15 -0.26081805888412646 1102 16-17 -0.0728519144175479 1102 18-19 -0.40191353771363936 1102 20-21 0.5741151990485065 1102 22-23 0.5084576853478069 1102 24-25 -4.5029548161571356 1102 26-27 -1.029703343246588 1102 28-29 0.4111226052595356 1102 30-31 1.6546960736179912 1102 32-33 0.5151004828098706 1102 34-35 0.7494996692216844 1102 36-37 -2.4536845158547465 1102 38-39 0.21364125223302466 1102 40-41 1.2043699983994358 1102 42-43 0.7946269071124945 1102 44-45 1.432651388995204 1102 46-47 1.1808120843057708 1102 48-49 -3.732775195478524 1102 50-51 -0.42262691204776104 1102 52-53 0.02825262506219417 1102 54-55 0.5321217431491334 1102 56-57 0.9166354021580787 1102 58-59 0.34162014249394446 1102 60-61 -5.927107947461913 1102 62-63 -2.5826942981553174 1102 64-65 0.18031072386786917 1102 66-67 1.1731522816582398 1102 68-69 -1.164694135837344 1102 70-71 -1.466793407609984 1102 72-73 -6.190114317190808 1102 74-75 -2.568842734220773 1102 76 0.11500404888208493 1103 1 1.3776213544953748 1103 2 0.8402280172602588 1103 3 0.40368550593788655 1103 4 0.5544241455237326 1103 5 0.4849134392979195 1103 6 0.5111889283730306 1103 7 1.136186049963051 1103 8 1.0301536059569116 1103 9 0.5297859544409178 1103 10-11 0.5624060615423829 1103 12-13 -0.2205555667027852 1103 14-15 0.33138240194536905 1103 16-17 0.6055697445686334 1103 18-19 1.1269574300282983 1103 20-21 0.49548616679044244 1103 22-23 0.9658655194491885 1103 24-25 -2.1325976732999976 1103 26-27 0.08300403002530032 1103 28-29 0.4747285960429437 1103 30-31 1.6469771796087684 1103 32-33 1.8963677639158618 1103 34-35 0.9394075033230607 1103 36-37 0.05913806479041028 1103 38-39 0.1936988559196564 1103 40-41 0.6601534085376883 1103 42-43 0.10529510987747415 1103 44-45 1.8190453982117916 1103 46-47 1.4246600105730494 1103 48-49 -1.495263674741203 1103 50-51 0.2750689404868041 1103 52-53 0.6226420259838576 1103 54-55 0.09466782609844415 1103 56-57 0.6130063699000132 1103 58-59 0.28296806876122105 1103 60-61 -3.575967394466524 1103 62-63 -0.8074408419341239 1103 64-65 0.46828307409828795 1103 66-67 0.31320988534486816 1103 68-69 1.061054712088925 1103 70-71 1.0291973758001518 1103 72-73 -4.482072842536432 1103 74-75 -1.6045339784604025 1103 76 -3.126931434988883 1104 1 0.36152250757528037 1104 2 0.41612401870915505 1104 3 0.5055107127074479 1104 4 -0.08775885811863304 1104 5 -0.009542520293969403 1104 6 0.5608136240042043 1104 7 0.9252710317512367 1104 8 0.637522853134584 1104 9 1.2194806800561935 1104 10-11 -0.2653262146333333 1104 12-13 0.4057398873853231 1104 14-15 0.712009452032909 1104 16-17 0.5146917537047244 1104 18-19 0.693304802897714 1104 20-21 0.1382527536336653 1104 22-23 0.4845603054351457 1104 24-25 1.0717067558952635 1104 26-27 1.5289320279324556 1104 28-29 1.8399108148665277 1104 30-31 1.1176873399935303 1104 32-33 0.31944546097580684 1104 34-35 0.3489319836321627 1104 36-37 1.5548416850186157 1104 38-39 1.411818108128223 1104 40-41 2.1519027494911427 1104 42-43 0.8311116232696989 1104 44-45 1.0630334850650769 1104 46-47 1.1539780231704029 1104 48-49 -0.2765306539938095 1104 50-51 0.6769364067295385 1104 52-53 0.8267351578132889 1104 54-55 0.28837720166441727 1104 56-57 0.6116681532497807 1104 58-59 -0.6165348793401151 1104 60-61 0.6484706551581709 1104 62-63 0.620406138525901 1104 64-65 0.8533674924268198 1104 66-67 -0.010155578603772142 1104 68-69 1.678264379446503 1104 70-71 1.6240799548354374 1104 72-73 1.3529642854292732 1104 74-75 0.5749782264779171 1104 76 -0.4461313222969565 1105 1 1.3226539446892502 1105 2 0.627540675540665 1105 3 0.3783813999886192 1105 4 -0.4483159217324655 1105 5 -0.38563914093207785 1105 6 0.30504038351480744 1105 7 0.9378010289973844 1105 8 -0.48732470184397414 1105 9 0.9729918331306635 1105 10-11 0.9410646740719599 1105 12-13 -0.28339029082782474 1105 14-15 0.4677630221969551 1105 16-17 0.39608952702389644 1105 18-19 0.49218556138546177 1105 20-21 0.7345881720214749 1105 22-23 0.23253426192438553 1105 24-25 0.8394100487077338 1105 26-27 0.6930218819786376 1105 28-29 1.1283072899442175 1105 30-31 1.6499663438882592 1105 32-33 1.2966770593864467 1105 34-35 -0.9650949253729166 1105 36-37 0.7440407093704806 1105 38-39 0.6196547657465388 1105 40-41 0.6265772056066439 1105 42-43 0.5328701503557411 1105 44-45 1.1902414790852944 1105 46-47 1.2659021743958547 1105 48-49 0.1867743540710194 1105 50-51 0.9350307456098932 1105 52-53 0.9994012737108449 1105 54-55 1.1516487701761662 1105 56-57 0.8678741408968165 1105 58-59 0.8246606830344803 1105 60-61 1.3579596201056532 1105 62-63 0.07126966580864291 1105 64-65 1.3079683815255265 1105 66-67 1.0116883176942721 1105 68-69 0.7561391974959903 1105 70-71 1.6822156013990224 1105 72-73 1.208849646773153 1105 74-75 0.027238346860308127 1105 76 0.9708599047379423 1106 1 -1.016777794742488 1106 2 0.1610126090125945 1106 3 0.3930037146109342 1106 4 0.07379080037425467 1106 5 -0.1522365075294445 1106 6 0.29152687000129873 1106 7 -0.8259550347586782 1106 8 0.7874500729308025 1106 9 -0.6585396984008653 1106 10-11 0.41074589375318027 1106 12-13 1.4665057590682196 1106 14-15 -0.02613857170464229 1106 16-17 0.4508019317363008 1106 18-19 0.845650564850466 1106 20-21 0.18597417340748024 1106 22-23 0.559803839193961 1106 24-25 -0.3164804571827773 1106 26-27 1.1883094772662375 1106 28-29 0.8013149129518382 1106 30-31 0.925849919771828 1106 32-33 0.6034338161432018 1106 34-35 1.2436022333242462 1106 36-37 1.4918924072221742 1106 38-39 0.4830643291561003 1106 40-41 1.2591135881430304 1106 42-43 1.0383448558304522 1106 44-45 1.2257924146362313 1106 46-47 1.101453109946796 1106 48-49 1.5667119840086556 1106 50-51 0.59019360077275 1106 52-53 0.8225474968570694 1106 54-55 0.4246217431491317 1106 56-57 0.8599046329273037 1106 58-59 1.2315560399298349 1106 60-61 1.0929561551021933 1106 62-63 0.9816646762036569 1106 64-65 -0.5035354299782853 1106 66-67 0.1359086919146435 1106 68-69 1.5151135564703537 1106 70-71 0.6565399257233508 1106 72-73 -0.24613995821645318 1106 74-75 -0.6333299137079536 1106 76 0.1432091770872148 1107 1 0.8384508475829264 1107 2 -0.20124663711762025 1107 3 0.21013711884111075 1107 4 0.31940110404907784 1107 5 -0.22476398005691323 1107 6 0.40058985003201997 1107 7 0.9795040223132858 1107 8 0.19605222346843476 1107 9 -0.14763340039779393 1107 10-11 0.7582585961045964 1107 12-13 0.15962876509445323 1107 14-15 0.40281097337393845 1107 16-17 0.8820674404211601 1107 18-19 0.8827177986918926 1107 20-21 0.790417042366478 1107 22-23 0.2815337222141707 1107 24-25 0.8650981792345647 1107 26-27 -0.14741071651847193 1107 28-29 1.4240373518033138 1107 30-31 1.0455946911295015 1107 32-33 1.1175659205978832 1107 34-35 0.3311125724474877 1107 36-37 1.4393223965876416 1107 38-39 0.6499200540763397 1107 40-41 0.9066971873395318 1107 42-43 1.2619771375272464 1107 44-45 1.1462343383039624 1107 46-47 1.127424987531576 1107 48-49 1.0692524542910533 1107 50-51 1.0653915211319642 1107 52-53 0.7816282010990676 1107 54-55 0.7467415588173338 1107 56-57 1.048490240867757 1107 58-59 1.0456408798211285 1107 60-61 1.4516823751187289 1107 62-63 1.1003932594483672 1107 64-65 1.3899420602733983 1107 66-67 0.37542186691168666 1107 68-69 1.738474066927644 1107 70-71 0.66975037119186 1107 72-73 1.317466327970486 1107 74-75 -0.2704325959719185 1107 76 1.2601653392046686 1108 1 0.022782644817951336 1108 2 0.5683386163386004 1108 3 0.05637367798089343 1108 4 0.361336587920043 1108 5 0.2752360199430868 1108 6 -0.07990170142727493 1108 7 -0.14353745234109283 1108 8 -0.17445468897395955 1108 9 -0.2394920793532478 1108 10-11 0.6748484578557523 1108 12-13 1.4895826821451408 1108 14-15 0.6985867030206308 1108 16-17 0.01893379986817223 1108 18-19 0.5930864622863652 1108 20-21 1.2568532942866 1108 22-23 0.3250053043954253 1108 24-25 0.8221642314619118 1108 26-27 -0.11791762896087477 1108 28-29 -0.6730440614071327 1108 30-31 0.1844579783798892 1108 32-33 1.13438619709558 1108 34-35 0.9342615739835836 1108 36-37 1.7545297698595306 1108 38-39 1.5823317284235046 1108 40-41 1.6336557126851545 1108 42-43 0.3420078594934495 1108 44-45 1.4311037699475904 1108 46-47 1.2472406557343376 1108 48-49 1.4872248045214675 1108 50-51 0.8916588022379557 1108 52-53 0.4269431012526752 1108 54-55 0.003193171720567989 1108 56-57 0.7886592116818818 1108 58-59 0.996024904398702 1108 60-61 1.4380111001571336 1108 62-63 1.3382580827970614 1108 64-65 0.8050726286297731 1108 66-67 1.411366567372518 1108 68-69 2.232329673686472 1108 70-71 0.6327304019138253 1108 72-73 -0.20273336480985193 1108 74-75 0.44615726577922743 1108 76 1.3197659536439872 1109 1 -1.1246827929700665 1109 2 0.4853893075828424 1109 3 0.3990772110070111 1109 4 0.37009234828870774 1109 5 -0.05425706761452176 1109 6 -0.27222120987581633 1109 7 -0.6241826136314188 1109 8 0.4218586750813387 1109 9 0.6219518530584267 1109 10-11 0.7052632043995288 1109 12-13 0.8808269217764817 1109 14-15 0.7046542913462943 1109 16-17 0.925846242264484 1109 18-19 0.8527638816412022 1109 20-21 1.2881128949010403 1109 22-23 0.7446673627671672 1109 24-25 -0.07499398666404744 1109 26-27 0.37102246320502985 1109 28-29 -0.7119073486575189 1109 30-31 0.4603412349083058 1109 32-33 1.304201865298353 1109 34-35 0.22281764157191475 1109 36-37 1.402456037140638 1109 38-39 1.3572933259657418 1109 40-41 0.8767432702888414 1109 42-43 1.3034517919051254 1109 44-45 1.0725016194099553 1109 46-47 1.2956277525085298 1109 48-49 0.9517409335537295 1109 50-51 0.6944237791964838 1109 52-53 0.0972964038640427 1109 54-55 -0.02514784210431742 1109 56-57 0.4862782593009385 1109 58-59 0.2806639212957833 1109 60-61 0.993157029496615 1109 62-63 -0.28209521981430896 1109 64-65 1.0051494335452915 1109 66-67 0.5620578116121493 1109 68-69 0.1831745277571315 1109 70-71 0.5568471453854045 1109 72-73 0.21838798695665673 1109 74-75 -0.24739112131754126 1109 76 0.5781376894350814 1110 1 -1.3571724785926698 1110 2 -0.7413098503779239 1110 3 0.015261735821830769 1110 4 -0.03215630661997437 1110 5 0.21876631164091265 1110 6 0.09431919111822396 1110 7 0.595953946312612 1110 8 0.6740120275555839 1110 9 0.8699070729778455 1110 10-11 -0.5644185578510559 1110 12-13 0.8257083365954045 1110 14-15 1.161551051063519 1110 16-17 0.8486271431740775 1110 18-19 -0.3726203439963598 1110 20-21 0.8885785498338521 1110 22-23 -0.6411627335003587 1110 24-25 -1.3670778528063536 1110 26-27 1.0358594839785482 1110 28-29 0.48158072044277844 1110 30-31 0.29592644011012936 1110 32-33 0.8287392262653697 1110 34-35 0.9842117111064965 1110 36-37 1.3761453270771895 1110 38-39 1.1349370637513445 1110 40-41 -0.3227765984591997 1110 42-43 -0.05068722901315681 1110 44-45 1.312193273811964 1110 46-47 0.8040843356146681 1110 48-49 1.591413286196861 1110 50-51 -0.018438430372363257 1110 52-53 0.6125327297742444 1110 54-55 -0.2471060055419656 1110 56-57 0.5375123655088672 1110 58-59 -0.9135238365636482 1110 60-61 -0.02636187416347724 1110 62-63 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-1.2778172812411128 2116 56-57 -0.9359052482484245 2116 58-59 -3.148806686774357 2116 60-61 -2.1242420938033746 2116 62-63 -0.4252146233585705 2116 64-65 -3.1017217964573334 2116 66-67 -1.871908693951518 2116 68-69 -3.3576819407153877 2116 70-71 0.3175155354794441 2116 72-73 0.10870682102056861 2116 74-75 -1.4115663114565464 2116 76 -3.4012561137195405 2117 1 0.4475946748931392 2117 2 0.6372609221030103 2117 3 0.039832324597441016 2117 4 -0.24442782310752165 2117 5 0.0709753683140093 2117 6 -0.1470696212267768 2117 7 -0.19140712652655623 2117 8 -0.5283394007534099 2117 9 -1.5760835580499872 2117 10-11 -1.161116454424949 2117 12-13 -1.9814699494338015 2117 14-15 -0.3984057781823722 2117 16-17 -1.8611413881017498 2117 18-19 -1.72533459034522 2117 20-21 -0.5278584851620138 2117 22-23 0.02876470289167088 2117 24-25 -1.735981131946616 2117 26-27 -2.6418524660535994 2117 28-29 -2.2625177456176573 2117 30-31 -1.234339014101316 2117 32-33 -2.481653903155049 2117 34-35 0.1338856341339607 2117 36-37 -1.9987283755038732 2117 38-39 -0.8236833091704838 2117 40-41 -1.2385247384426705 2117 42-43 -1.3945836192032957 2117 44-45 -1.3121293127591755 2117 46-47 -1.666293178852122 2117 48-49 -1.358389230566246 2117 50-51 -0.9375391927495116 2117 52-53 0.17969999348325416 2117 54-55 -1.0771326428157764 2117 56-57 -0.5506891592454295 2117 58-59 -2.16324827855869 2117 60-61 -1.3236430351812132 2117 62-63 -1.3400627192079497 2117 64-65 -1.6567068199917827 2117 66-67 -1.7217161393943954 2117 68-69 -0.7175450130303247 2117 70-71 -1.7083723549784011 2117 72-73 0.4247102442126973 2117 74-75 0.783876564024844 2117 76 -0.5428906879600177 2118 1 -1.6965599594804033 2118 2 -0.427868716151913 2118 3 -1.8065424619263943 2118 4 -0.1902436902089093 2118 5 0.24299834611248627 2118 6 -0.6966737199692048 2118 7 0.598687579264471 2118 8 0.15323178384525704 2118 9 -3.737005775097032 2118 10-11 -1.710992250108852 2118 12-13 -1.7838686452884787 2118 14-15 -0.1885392978221816 2118 16-17 -4.056413861320202 2118 18-19 -1.5958515908109803 2118 20-21 -1.1874998451992838 2118 22-23 -0.0719184208468775 2118 24-25 -0.3506760550951924 2118 26-27 -4.226893608733313 2118 28-29 -2.120536686775864 2118 30-31 -1.5981800325766997 2118 32-33 -0.8042358003759702 2118 34-35 -0.9700578529022081 2118 36-37 -5.133839383111386 2118 38-39 -2.0752746769705155 2118 40-41 -2.7908069927510013 2118 42-43 -0.8298421194361723 2118 44-45 -3.8069282570224914 2118 46-47 -0.9436569422429031 2118 48-49 -1.0729521866289744 2118 50-51 -2.7093525757645693 2118 52-53 -3.7465925076811644 2118 54-55 -2.623165867470334 2118 56-57 -0.3443159252755521 2118 58-59 -2.2313665831697804 2118 60-61 -1.908722991709702 2118 62-63 -2.9944642096597462 2118 64-65 -1.1638485681675306 2118 66-67 -0.24337426701433174 2118 68-69 -2.4267516579612334 2118 70-71 -2.1343155315037876 2118 72-73 0.04355824918086881 2118 74-75 -0.5582675129818355 2118 76 -0.7389782520028731 2119 1 0.28651890855421414 2119 2 -2.1019910539910676 2119 3 -0.22091569930847754 2119 4 -1.4111908846074286 2119 5 -0.7412474965404314 2119 6 -2.217630639156212 2119 7 -1.0655154743191133 2119 8 -0.3590700735893435 2119 9 -0.26000489986606823 2119 10-11 -1.2064702234629365 2119 12-13 -1.1675601749977176 2119 14-15 -2.388043333609403 2119 16-17 -1.427220046285676 2119 18-19 -1.6975728783729807 2119 20-21 -2.356516669083362 2119 22-23 -1.0610752816851559 2119 24-25 0.03773199702967389 2119 26-27 -0.6256099366531842 2119 28-29 -0.9602235485866188 2119 30-31 0.4676081615300731 2119 32-33 -0.8211083083989195 2119 34-35 -0.6369838472618383 2119 36-37 -0.37953616420639946 2119 38-39 -2.9707818246900572 2119 40-41 -1.665794836765393 2119 42-43 -0.6774060599204645 2119 44-45 -1.2569914681476533 2119 46-47 -0.8956164871228012 2119 48-49 -1.568819151522483 2119 50-51 -0.3050444944653421 2119 52-53 -0.6107858364762606 2119 54-55 -0.29296067443328155 2119 56-57 -3.304930531907857 2119 58-59 -1.345000736626936 2119 60-61 -0.7645530024069629 2119 62-63 -1.585001990463013 2119 64-65 -1.6738651003079568 2119 66-67 0.1926852486911983 2119 68-69 0.39533333669013615 2119 70-71 -1.0163538471704214 2119 72-73 -0.25236706444355406 2119 74-75 -0.21867789905594037 2119 76 -0.74763331375528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 4.0 22 8.0 23 26.0 24 41.0 25 68.0 26 138.0 27 264.0 28 462.0 29 754.0 30 1233.0 31 2078.0 32 3041.0 33 4746.0 34 7002.0 35 10199.0 36 15701.0 37 19247.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.90820297478965 24.59969544552628 12.300616799716979 27.191484779967084 2 17.80566963530371 19.497638933751713 38.44923323027702 24.24745820066756 3 17.138110839370587 26.0747850429914 29.80942273083845 26.977681386799564 4 13.203513143525141 19.55455062833587 37.96163844154246 29.280297786596527 5 13.378862689000663 37.300232261240055 36.31581376032486 13.00509128943442 6 27.851352806361806 38.273883684801504 18.31172227093043 15.563041237906264 7 27.356067248088845 30.229338747635087 22.896959069724517 19.517634934551552 8 23.319951394336517 35.9974159014351 21.120391306354115 19.56224139787427 9 23.29226462399828 16.129081875932506 22.904649839262916 37.6740036608063 10-11 24.731207604632917 25.94942549951548 27.366065248488763 21.953301647362835 12-13 24.39665912971252 23.33456385645948 26.858474458954362 25.410302554873642 14-15 21.899466260594036 25.55488902219556 23.40531893621276 29.140325780997646 16-17 22.096349960777076 28.19436112777445 25.976343192899883 23.7329457185486 18-19 22.266315967575714 27.295310168735483 26.53161675357236 23.906757110116438 20-21 21.355728854229152 27.292233860920124 26.242443818928525 25.1095934659222 22-23 22.363988740713395 27.94594927168412 27.000753695414765 22.689308292187714 24-25 22.260163351944996 28.655807300078447 26.655438143140604 22.428591204835957 26-27 21.445710857828434 28.052081891314046 27.915955270484368 22.586251980373156 28-29 21.624136711119316 26.437789365203884 29.141863934905327 22.796209988771476 30-31 21.679510251795794 26.570070601264362 29.330287788596127 22.420131358343713 32-33 21.787181025333393 27.55295094827188 26.976912309845723 23.682955716549 34-35 21.704889791272514 27.269930629258766 28.479688677649083 22.545490901819637 36-37 21.945610877824436 27.972097888114682 27.31222986171996 22.770061372340916 38-39 21.597988094688755 27.36452709458108 27.856736345038684 23.18074846569148 40-41 21.256517927183793 26.795410148739485 28.715795302477964 23.232276621598757 42-43 22.028671188839155 26.193222893882762 29.18647039822805 22.591635519050037 44-45 20.750465291557074 26.361650746773723 28.727331456785564 24.16055250488364 46-47 21.964068724716597 25.476443172903878 28.460461753803084 24.099026348576437 48-49 20.64279451801947 25.60718625505668 29.35336009721133 24.39665912971252 50-51 20.21211142386907 25.825604109947243 29.74866565148509 24.2136188146986 52-53 19.94754895174811 25.97403596203836 30.86075092673773 23.2176641594758 54-55 18.72933105686555 26.440096596065406 30.14012582098965 24.6904465260794 56-57 19.96062325996339 26.24859643455924 29.391813944903326 24.39896636057404 58-59 19.120022149416272 26.854629074185166 29.495639333671726 24.529709442726837 60-61 19.686062787442513 27.722916955070527 28.224355128974203 24.36666512851276 62-63 18.080999184778427 28.523526064017968 27.916724347438205 25.4787504037654 64-65 18.92083121837171 29.247227477581404 26.661590758771325 25.17035054527556 66-67 18.97774291295587 28.874994231922845 26.2939719748358 25.853290880285478 68-69 18.641656284127787 28.851921923307643 25.9940319628382 26.512389829726363 70-71 19.22769292295387 28.256656361035486 26.097088274652762 26.418562441357885 72-73 19.12694384200083 27.554489102179563 26.269361512312923 27.049205543506684 74-75 19.05080522357067 27.65754541399412 26.521618753172444 26.77003060926276 76 19.64222540107363 27.105348161137005 26.419331518311722 26.83309491947764 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 2.0 19 8.0 20 16.5 21 20.0 22 21.5 23 37.0 24 57.0 25 63.0 26 117.5 27 201.0 28 274.0 29 318.0 30 403.0 31 579.5 32 826.0 33 981.0 34 1173.5 35 1484.5 36 1785.0 37 1967.0 38 2290.5 39 3099.5 40 4011.0 41 4521.5 42 4606.0 43 4714.0 44 4948.0 45 5078.5 46 5083.0 47 5027.0 48 4729.5 49 4192.0 50 3896.0 51 3549.0 52 2844.0 53 2245.5 54 2005.0 55 1847.0 56 1491.5 57 1069.5 58 845.0 59 707.5 60 496.0 61 356.5 62 291.0 63 258.5 64 188.0 65 134.5 66 99.0 67 79.0 68 60.0 69 33.5 70 19.0 71 12.0 72 8.0 73 3.0 74 1.0 75 0.0 76 0.5 77 1.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 65013.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.336686508852075 #Duplication Level Percentage of deduplicated Percentage of total 1 65.61169780666125 31.05840370387461 2 14.157595450852964 13.403473151523542 3 7.181153533712428 10.197960407918416 4 4.097481722177092 7.758448310337933 5 2.420796100731113 5.729623306108009 6 1.718927701056052 4.882100502976328 7 1.075548334687246 3.563902604094565 8 0.9065800162469537 3.4331595219417657 9 0.6043866774979691 2.574869641456324 >10 2.2095857026807475 15.333856305661945 >50 0.006498781478472787 0.1922692384600003 >100 0.0032493907392363935 0.1799640071985603 >500 0.006498781478472787 1.6919692984480026 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 579 0.8905911125467214 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 521 0.8013781859012813 RNA PCR Primer, Index 48 (95% over 23bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 117 0.1799640071985603 No Hit CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC 66 0.10151815790688015 RNA PCR Primer, Index 48 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003076307815360005 0.0 2 0.0 0.0 0.0 0.003076307815360005 0.0 3 0.0 0.0 0.0 0.003076307815360005 0.0 4 0.0 0.0 0.0 0.003076307815360005 0.0 5 0.0 0.0 0.0 0.00615261563072001 0.0 6 0.0 0.0 0.0 0.00615261563072001 0.0 7 0.0 0.0 0.0 0.007690769538400012 0.0 8 0.0 0.0 0.0 0.007690769538400012 0.0 9 0.0 0.0 0.0 0.013843385169120022 0.0 10 0.0 0.0 0.0 0.026148616430560042 0.0 11 0.0 0.0 0.0 0.03230123206128005 0.0 12 0.0 0.0 0.0 0.03691569378432006 0.0 13 0.0 0.0 0.0 0.03845384769200006 0.0 14 0.0 0.0 0.0 0.04153015550736007 0.0 15 0.0 0.0 0.0 0.04460646332272007 0.0 16 0.0 0.0 0.0 0.04460646332272007 0.0 17 0.0 0.0 0.0 0.04614461723040007 0.0 18 0.0 0.0 0.0 0.052297232861120084 0.0 19 0.0 0.0 0.0 0.058449848491840095 0.0 20 0.0 0.0 0.0 0.058449848491840095 0.0 21 0.0 0.0 0.0 0.0615261563072001 0.0 22 0.0 0.0 0.0 0.0646024641225601 0.0 23 0.0 0.0 0.0 0.0676787719379201 0.0 24 0.0 0.0 0.0 0.0676787719379201 0.0 25 0.0 0.0 0.0 0.06921692584560012 0.0 26 0.0 0.0 0.0 0.06921692584560012 0.0 27 0.0 0.0 0.0 0.09075108055312014 0.0 28 0.0 0.0 0.0 0.11536154307600019 0.0 29 0.0 0.0 0.0 0.1261286204297602 0.0 30 0.0 0.0 0.0 0.16458246812176025 0.0 31 0.0 0.0 0.0 0.20303631581376033 0.0 32 0.0 0.0 0.0 0.23072308615200038 0.0 33 0.0 0.0 0.0 0.27532954947472044 0.0 34 0.0 0.0 0.0 0.3122452432590405 0.0 35 0.0 0.0 0.0 0.3676187839355206 0.0 36 0.0 0.0 0.0 0.5014381739036808 0.0 37 0.0 0.0 0.0 0.7890729546398413 0.0 38 0.0 0.0 0.0 1.0767077353760017 0.0 39 0.0 0.0 0.0 1.5012382138956823 0.0 40 0.0 0.0 0.0 2.0057526956147234 0.0 41 0.0 0.0 0.0 2.650239182932644 0.0 42 0.0 0.0 0.0 3.4423884453878455 0.0 43 0.0 0.0 0.0 4.340670327472967 0.0 44 0.0 0.0 0.0 5.120514358666728 0.0 45 0.0 0.0 0.0 5.90804915939889 0.0 46 0.0 0.0 0.0 6.612523649116331 0.0 47 0.0 0.0 0.0 7.450817528801932 0.0 48 0.0 0.0 0.0 8.327565256179533 0.0 49 0.0 0.0 0.0 9.047421284973774 0.0 50 0.0 0.0 0.0 9.679602541030256 0.0 51 0.0 0.0 0.0 10.465599187854737 0.0 52 0.0 0.0 0.0 11.302354913632659 0.0 53 0.0 0.0 0.0 12.125267254241459 0.0 54 0.0 0.0 0.0 12.85896666820482 0.0 55 0.0 0.0 0.0 13.675726393182902 0.0 56 0.0 0.0 0.0 14.363281189915863 0.0 57 0.0 0.0 0.0 15.053912294464183 0.0 58 0.0 0.0 0.0 15.733776321658745 0.0 59 0.0 0.0 0.0 16.373648347253628 0.0 60 0.0 0.0 0.0 16.948917908725946 0.0 61 0.0 0.0 0.0 17.545721624905788 0.0 62 0.0 0.0 0.0 18.207127805208188 0.0 63 0.0 0.0 0.0 18.88237737067971 0.0 64 0.0 0.0 0.0 19.62530570808915 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGAGG 15 0.0022154474 70.0 4 TAACGAG 15 0.0022154474 70.0 65 GGTATGA 15 0.0022154474 70.0 41 TTAACGA 15 0.0022154474 70.0 64 TTCGGTC 15 0.0022154474 70.0 28 ATTGGCG 15 0.0022154474 70.0 21 GGTTAAC 15 0.0022154474 70.0 62 TCGGTCA 15 0.0022154474 70.0 29 CGCCATT 15 0.0022154474 70.0 17 GAACCCT 25 2.3742356E-4 55.999996 3 GGAATGA 20 0.006922713 52.500004 40 GAGGACT 20 0.006922713 52.500004 33 CTCGTGG 20 0.006922713 52.500004 8 TATGGGT 20 0.006922713 52.500004 6 GCTATAA 20 0.006922713 52.500004 1 CGTCTTT 20 0.006922713 52.500004 25 TACGGTG 20 0.006922713 52.500004 55 TTCAAAT 20 0.006922713 52.500004 28 CCTCGTG 20 0.006922713 52.500004 7 CGACAAT 20 0.006922713 52.500004 17 >>END_MODULE