FastQCFastQC Report
Wed 25 May 2016
SRR1780593_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780593_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857837
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAGT207.900977E-443.94836
CTGGTAC250.002345119135.22228234
GGCTACC300.00575418829.32279430
CGTTGTT655.8951264E-727.09406334
TTTTACG655.949478E-727.06561329
TCGGTGG1006.184564E-1126.3812842
CGGTGGT701.139224E-625.12356443
GTCTAGC450.001395582224.4513531
ACGTGCT450.001403670324.42711342
GCTATAC551.605846E-423.9717983
TTCGGTA752.0706066E-623.45686726
GACGGAG602.843791E-422.0319419
ATAAACG500.002562532422.02550316
CTCGCTA1105.2204996E-922.0100713
TTACGTT803.632138E-621.98696331
GGACACT904.1253952E-721.974156
CGTGCGA1158.774805E-921.04082910
GTATAGA2200.021.0059341
TAAGACT1057.836206E-820.927769
CCGATGC956.766495E-720.87236418
Produced by FastQC (version 0.11.5)