FastQCFastQC Report
Wed 25 May 2016
SRR1780593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857837
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT149151.73867529612269No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT59790.6969855578623911Illumina PCR Primer Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC22270.2596064287271358No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC14430.168213774877978No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG11530.1344078187347946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTATA207.847626E-444.0088042
CGTGTAC207.861157E-443.99341239
CCGTCTT6600.037.99652544
GCGCAAA250.00235414135.19473334
GCGTGTA250.00235414135.19473338
GCCGTCT7250.034.58994343
ACCGTGG300.00573520929.34262813
TGCCACG300.005748297629.32894529
TGCGTGT300.005748297629.32894537
CGCTTGG300.005748297629.32894535
GTTGCGT300.005748297629.32894535
TTCGGTA1250.028.15742726
ATGCCGT24850.027.79625344
TATGCCG25000.027.62947743
AAGACGA407.0366106E-427.4958828
TGCCGTC9150.027.40573142
ATTGGCG2450.026.93631421
GGCTATA1250.026.3960530
TCGTTCG1350.026.07169223
TTCAGCC2150.025.57905644
Produced by FastQC (version 0.11.5)