FastQCFastQC Report
Wed 25 May 2016
SRR1780592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3710
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATC50.13477088948787064No Hit
CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG50.13477088948787064No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTGACGCTGCCGACG40.1078167115902965No Hit
CTCTTCCTTTGGTCCTATTTCTGTTATATAAGACAATATAATCATTTATT40.1078167115902965No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG40.1078167115902965No Hit
CATCAAAGGCCTTTTAAAATTAGAAGAGGATAAGGGAAATTCTAAGACTC40.1078167115902965No Hit
ATAATATAATCATTGGGCATGAAATAGATGCAACTTAAGGCTTTTCAATA40.1078167115902965No Hit
CATCCTATTCATTCTTTATCTCTGGGCAACCATGACTAGCTAATACAGTC40.1078167115902965No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGC200.00641956652.563
Produced by FastQC (version 0.11.5)