##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780592_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3710 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.750943396226415 23.0 12.0 32.0 12.0 34.0 2 21.26118598382749 21.0 11.0 32.0 11.0 34.0 3 19.921832884097036 21.0 11.0 31.0 11.0 34.0 4 20.71832884097035 21.0 11.0 32.0 11.0 34.0 5 19.685175202156334 21.0 11.0 31.0 11.0 34.0 6 22.86199460916442 23.0 11.0 34.0 11.0 37.0 7 21.980323450134772 21.0 11.0 34.0 11.0 37.0 8 23.22156334231806 26.0 11.0 34.0 11.0 37.0 9 23.326415094339623 23.0 12.0 34.0 12.0 37.0 10-11 22.123719676549864 21.0 11.0 34.0 11.0 37.0 12-13 21.979380053908358 21.0 11.0 34.0 11.0 37.0 14-15 21.658086253369273 21.0 11.0 33.5 11.0 37.0 16-17 21.351347708894878 21.0 11.0 32.0 11.0 37.0 18-19 21.4588948787062 21.0 11.0 32.0 11.0 37.0 20-21 21.519407008086255 21.0 11.0 32.0 11.0 37.0 22-23 21.19851752021563 21.0 11.0 32.0 11.0 37.0 24-25 21.040026954177897 21.0 11.0 31.5 10.5 37.0 26-27 20.986927223719675 21.0 11.0 31.0 10.5 37.0 28-29 20.487735849056605 16.0 11.0 31.0 11.0 37.0 30-31 20.375606469002697 21.0 11.0 31.0 11.0 37.0 32-33 20.734905660377358 21.0 11.0 31.0 11.0 37.0 34-35 21.13800539083558 21.0 11.0 31.0 11.0 37.0 36-37 20.73463611859838 21.0 11.0 31.0 11.0 37.0 38-39 20.216711590296498 16.0 11.0 27.0 11.0 37.0 40-41 20.535175202156335 21.0 11.0 29.0 11.0 36.5 42-43 20.735040431266846 21.0 11.0 31.0 11.0 37.0 44-45 20.59568733153639 21.0 11.0 31.0 11.0 37.0 46-47 20.766711590296495 21.0 11.0 31.0 11.0 37.0 48-49 20.935579514824795 21.0 11.0 31.0 11.0 37.0 50-51 21.159433962264153 21.0 11.0 31.0 11.0 37.0 52-53 21.036927223719676 21.0 11.0 31.0 11.0 37.0 54-55 21.060916442048516 21.0 11.0 31.0 11.0 37.0 56-57 20.397169811320754 20.0 11.0 29.0 11.0 36.5 58-59 20.32412398921833 20.0 11.0 29.0 11.0 36.0 60-61 20.6588948787062 21.0 11.0 31.0 11.0 36.0 62-63 20.164420485175203 19.5 11.0 28.5 11.0 36.0 64-65 20.267520215633425 20.0 11.0 29.0 11.0 36.0 66-67 20.833153638814018 20.0 11.0 31.0 11.0 36.5 68-69 20.21522911051213 19.0 11.0 28.5 11.0 36.0 70-71 19.956603773584906 19.0 11.0 27.0 10.0 36.0 72-73 20.44636118598383 19.5 11.0 28.0 10.5 36.0 74-75 20.513746630727763 20.0 11.0 29.5 11.0 36.0 76 20.112938005390834 19.0 11.0 27.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -2.364160291462923 1101 2 1.8352499109078053 1101 3 7.098329923000978 1101 4 0.4652327204958695 1101 5 -2.0035200545068967 1101 6 0.8765899670504993 1101 7 -1.4662810508205233 1101 8 -0.058318143844456216 1101 9 5.3465358106805425 1101 10-11 3.1303230577901644 1101 12-13 1.7591415711481524 1101 14-15 6.845020202750465 1101 16-17 3.7761754327543784 1101 18-19 0.2014324126166187 1101 20-21 5.791247732530625 1101 22-23 3.0111633797489006 1101 24-25 4.470156123116642 1101 26-27 3.805952143616615 1101 28-29 1.2743312243312168 1101 30-31 5.618419719899986 1101 32-33 3.842152474718265 1101 34-35 5.201389071454859 1101 36-37 3.8784079590000644 1101 38-39 5.1595906651827725 1101 40-41 4.071947533460694 1101 42-43 2.2349762080025286 1101 44-45 7.427125140940934 1101 46-47 7.662223065677015 1101 48-49 0.10352259217390625 1101 50-51 4.960411116661113 1101 52-53 2.2924095093503034 1101 54-55 4.0794854268538465 1101 56-57 3.4474417760601987 1101 58-59 2.4636451633162153 1101 60-61 1.5149396290843633 1101 62-63 2.737227282950972 1101 64-65 0.5886750493658361 1101 66-67 4.870166237271508 1101 68-69 0.824136901110581 1101 70-71 1.9349477459674844 1101 72-73 0.45380346992189047 1101 74-75 0.3681397732384575 1101 76 1.8838985940959638 1102 1 -0.21730714460977651 1102 2 0.18490026055815534 1102 3 0.07035789502894829 1102 4 -1.9753267200635705 1102 5 -4.13638718737403 1102 6 -4.214319123858591 1102 7 -4.997749582289053 1102 8 -6.4009754865018 1102 9 -0.7443732802285474 1102 10-11 4.081372008839111 1102 12-13 0.19620450821108903 1102 14-15 -1.403231545501285 1102 16-17 -0.7797686231896819 1102 18-19 2.3098240210082253 1102 20-21 -5.558402617119725 1102 22-23 0.6789955475810707 1102 24-25 -5.131242478281958 1102 26-27 -2.774467436802965 1102 28-29 0.5785270285270236 1102 30-31 -0.3151467136664472 1102 32-33 -1.273232140666348 1102 34-35 -3.0713382012724146 1102 36-37 -3.1705430899509857 1102 38-39 -3.2599897543976475 1102 40-41 -7.0364440749309125 1102 42-43 -0.9992895262632047 1102 44-45 -4.11483290101711 1102 46-47 -3.6105042070502584 1102 48-49 -2.1901837015323906 1102 50-51 -5.382246225996228 1102 52-53 -2.9558422389014503 1102 54-55 -4.133801286432867 1102 56-57 -2.5455652169467946 1102 58-59 -2.8160751164040647 1102 60-61 -3.3067386925939637 1102 62-63 -1.4026328569091682 1102 64-65 -0.09314313245234374 1102 66-67 -3.1088547417494716 1102 68-69 -4.508030931057252 1102 70-71 -0.43917812815838886 1102 72-73 -0.3294133132948929 1102 74-75 -2.673818268719586 1102 76 -5.815402105204736 1103 1 0.22371849641586294 1103 2 2.5746438503017437 1103 3 2.1165117411827943 1103 4 -0.0984036431404931 1103 5 -0.8338230848099251 1103 6 4.6523475428080765 1103 7 0.8125068279673577 1103 8 3.5477424622161493 1103 9 4.255626719771453 1103 10-11 -0.0878587603916543 1103 12-13 4.416717328723909 1103 14-15 2.148050505780766 1103 16-17 4.479205735784678 1103 18-19 2.2226445338287384 1103 20-21 2.7518537931366858 1103 22-23 0.5687391373246626 1103 24-25 0.29439854735906934 1103 26-27 2.3332248708893424 1103 28-29 -2.9650627150627216 1103 30-31 0.7335712350514996 1103 32-33 -3.0578475252817334 1103 34-35 -0.7713382012724175 1103 36-37 0.5602261408182443 1103 38-39 0.8323179379100445 1103 40-41 0.7840687455819086 1103 42-43 2.228915601941921 1103 44-45 0.32106453488032827 1103 46-47 0.989495792949743 1103 48-49 -2.305568316917004 1103 50-51 -2.466861610611616 1103 52-53 0.7621064790472722 1103 54-55 -1.1235448761764566 1103 56-57 1.892896321514744 1103 58-59 -0.7878699881989384 1103 60-61 -0.009302795158063759 1103 62-63 0.4948030405267261 1103 64-65 -0.6931431324523452 1103 66-67 0.2065298736351444 1103 68-69 0.015045992019672383 1103 70-71 0.4864628974826388 1103 72-73 -0.10377228765386803 1103 74-75 -2.2045874994888166 1103 76 -0.5948892846919165 1104 1 0.47500054769791333 1104 2 3.1079771836350787 1104 3 -3.6988728742018218 1104 4 -0.7445574892943405 1104 5 -2.2902333412201834 1104 6 -4.445088354627821 1104 7 -4.61313419767367 1104 8 1.3682552827289705 1104 9 -2.5136040494593175 1104 10-11 0.6198335473006509 1104 12-13 -1.9961031840966044 1104 14-15 -3.1724623147320514 1104 16-17 -3.2028455462666017 1104 18-19 0.7713624825466887 1104 20-21 -5.096864155581265 1104 22-23 -0.5902352216496993 1104 24-25 -2.400473247512725 1104 26-27 -1.1206212829568116 1104 28-29 -0.9983960483960566 1104 30-31 -0.9305313290510675 1104 32-33 -2.9655398329740414 1104 34-35 -0.494415124349338 1104 36-37 -1.324389243797139 1104 38-39 -4.106143600551494 1104 40-41 -5.497982536469376 1104 42-43 -0.30698183395551126 1104 44-45 -2.1917559779401863 1104 46-47 -2.3412734378194884 1104 48-49 -2.074799086147774 1104 50-51 -1.88224622599623 1104 52-53 1.5056962226370132 1104 54-55 -3.172262824894407 1104 56-57 -2.1224882938698713 1104 58-59 -2.969921270250218 1104 60-61 -0.6528925387478068 1104 62-63 -2.7872482415245514 1104 64-65 -0.09314313245234374 1104 66-67 0.698837565942835 1104 68-69 -4.123415546441869 1104 70-71 -1.8622550512353087 1104 72-73 -0.29095177483335277 1104 74-75 -1.519972114873429 1104 76 -3.815402105204736 1105 1 -0.09711483691746992 1105 2 0.9829771836350787 1105 3 0.479011741182795 1105 4 0.8515963568595062 1105 5 -3.80465641814326 1105 6 2.5981808761414094 1105 7 -8.108326505365977 1105 8 -4.035590871117183 1105 9 0.8181267197714526 1105 10-11 -2.5316087603916557 1105 12-13 -2.1811993379427577 1105 14-15 0.012633839114101875 1105 16-17 -2.7749609308819885 1105 18-19 -3.844022132837928 1105 20-21 -1.1377295401966485 1105 22-23 -2.991677529342006 1105 24-25 0.06106521402573506 1105 26-27 -2.9980251291106583 1105 28-29 -3.0608960483960566 1105 30-31 -1.356012098281834 1105 32-33 -1.2203475252817348 1105 34-35 -3.3213382012724146 1105 36-37 -1.1897738591817557 1105 38-39 -1.567682062089954 1105 40-41 -3.945097921084759 1105 42-43 -1.7252510647247448 1105 44-45 -5.09560213178634 1105 46-47 -2.8292542070502584 1105 48-49 -3.899318316917004 1105 50-51 -4.079361610611613 1105 52-53 -3.2899768542860635 1105 54-55 -1.3693782095097902 1105 56-57 -0.9446036784852545 1105 58-59 -2.292036654865605 1105 60-61 -1.5759694618247302 1105 62-63 -0.6093636261399382 1105 64-65 0.25060686754765626 1105 66-67 -2.9934701263648584 1105 68-69 -4.272454007980329 1105 70-71 -1.2156204358506955 1105 72-73 -2.2621056209871995 1105 74-75 1.001662500511184 1105 76 -4.849055951358583 1106 1 -0.9096148369174699 1106 2 -1.738176662518768 1106 3 0.8395886642597183 1106 4 3.8708271260902762 1106 5 -0.59792564891249 1106 6 3.0164501069106393 1106 7 4.540711956172483 1106 8 1.7528706673443573 1106 9 2.4863959505406825 1106 10-11 5.812141239608344 1106 12-13 1.8885122005187824 1106 14-15 6.519845377575642 1106 16-17 1.4125390691180115 1106 18-19 0.11751632870053186 1106 20-21 4.441597382880275 1106 22-23 5.909764778350301 1106 24-25 4.791834444794965 1106 26-27 1.687071024735495 1106 28-29 2.3862193362193302 1106 30-31 3.838699440179706 1106 32-33 5.0344601670259586 1106 34-35 7.544046414112202 1106 36-37 4.560226140818244 1106 38-39 4.086164091756199 1106 40-41 4.925094386607547 1106 42-43 4.577633550659868 1106 44-45 5.769782483598274 1106 46-47 2.081803485257435 1106 48-49 2.655970144621456 1106 50-51 2.38698454323454 1106 52-53 3.4672346841754766 1106 54-55 1.4431217904902098 1106 56-57 0.8390501676685922 1106 58-59 0.7223864220574718 1106 60-61 1.8471074612521932 1106 62-63 2.520444066167755 1106 64-65 0.9453184060091964 1106 66-67 2.5065298736351416 1106 68-69 0.030430607404287713 1106 70-71 3.2915911026108446 1106 72-73 2.3244328405512604 1106 74-75 3.9031048082034907 1106 76 0.10767481787218713 1107 1 1.257051829749198 1107 2 -1.0586894830315892 1107 3 -3.416821592150537 1107 4 -2.0650703098071617 1107 5 -1.4227119736988172 1107 6 -0.3809857905252585 1107 7 -0.8652709498104194 1107 8 1.9366313511050386 1107 9 2.3667378308825633 1107 10-11 -2.7156365381694343 1107 12-13 0.18338399539057804 1107 14-15 2.0091616168918804 1107 16-17 0.634761291340233 1107 18-19 -1.4829110217268138 1107 20-21 -1.2314795401966485 1107 22-23 0.6576280262135512 1107 24-25 0.31106521402573506 1107 26-27 0.7554470931115631 1107 28-29 -1.6928404928404994 1107 30-31 -2.5886509871707233 1107 32-33 0.9810413636071544 1107 34-35 -1.3213382012724146 1107 36-37 0.5324483630404657 1107 38-39 -1.1510153954232898 1107 40-41 -0.3270423655292021 1107 42-43 1.2122489352752552 1107 44-45 0.18217564599143898 1107 46-47 0.16727357072752014 1107 48-49 1.0555427941941105 1107 50-51 -0.8224171661671704 1107 52-53 -1.1545601876193992 1107 54-55 -1.6124337650653473 1107 56-57 1.020674099292524 1107 58-59 -4.521203321532273 1107 60-61 0.34069720484193766 1107 62-63 1.2448030405267296 1107 64-65 1.2957457564365455 1107 66-67 0.3120854291906987 1107 68-69 -1.501620674646997 1107 70-71 0.14201845303819383 1107 72-73 -1.9982167320983137 1107 74-75 -3.737920832822148 1107 76 -5.383778173580804 1108 1 6.55192362462099 1108 2 0.261823337481232 1108 3 1.147280971952025 1108 4 0.5631348183979661 1108 5 1.0943820433951998 1108 6 4.631834722295256 1108 7 0.5407119561724834 1108 8 3.5990245134982004 1108 9 4.101780565925299 1108 10-11 3.4659873934544976 1108 12-13 6.119281431288012 1108 14-15 1.019845377575642 1108 16-17 4.181769838348782 1108 18-19 3.925208636392842 1108 20-21 3.672366613649505 1108 22-23 -0.936389067803546 1108 24-25 0.02260367556419496 1108 26-27 0.8024556401201117 1108 28-29 1.5016039516039434 1108 30-31 2.800237901718166 1108 32-33 2.803690936256725 1108 34-35 2.8902002602660453 1108 36-37 1.906379986972091 1108 38-39 4.7400102456023525 1108 40-41 1.0020174635306276 1108 42-43 1.0391720121983319 1108 44-45 3.269782483598277 1108 46-47 0.38949579294974157 1108 48-49 1.079047067698383 1108 50-51 0.5408306970806933 1108 52-53 1.3903116072523964 1108 54-55 1.7123525597209799 1108 56-57 3.762127090745512 1108 58-59 4.376232575903625 1108 60-61 1.039415153559883 1108 62-63 3.443520989244675 1108 64-65 4.63762609831689 1108 66-67 3.929606796712065 1108 68-69 1.1842767612504375 1108 70-71 -0.1699473589276188 1108 72-73 0.7475097636281873 1108 74-75 0.9800278851265709 1108 76 9.107674817872187 1109 1 0.8596159323133001 1109 2 2.261823337481232 1109 3 -2.083488258817205 1109 4 2.8708271260902762 1109 5 1.40207435108751 1109 6 0.4010654915260261 1109 7 -0.7669803515198232 1109 8 -5.4009754865018 1109 9 2.640242104386836 1109 10-11 0.2736797011468042 1109 12-13 -0.9576416456350643 1109 14-15 -1.8263084685782047 1109 16-17 -0.2797686231896819 1109 18-19 -3.1901759789917747 1109 20-21 -2.173787232504342 1109 22-23 0.6789955475810707 1109 24-25 -1.938934785974265 1109 26-27 0.7255325631970351 1109 28-29 -3.306088356088363 1109 30-31 -3.0074544059741406 1109 32-33 0.9190755516413418 1109 34-35 1.1209694910352788 1109 36-37 -2.593620013027909 1109 38-39 -0.5676820620899541 1109 40-41 0.34817130968447074 1109 42-43 -3.153135680109358 1109 44-45 -0.15329443947864618 1109 46-47 1.0048804083343548 1109 48-49 0.8867393753906896 1109 50-51 -0.4591693029193067 1109 52-53 1.15954237648317 1109 54-55 -0.2876474402790201 1109 56-57 -2.5455652169467946 1109 58-59 -1.5853058856348383 1109 60-61 0.8855689997137297 1109 62-63 -0.13340208767839812 1109 64-65 0.21454917523996286 1109 66-67 -0.6857778186725518 1109 68-69 -0.08495400798032904 1109 70-71 -3.9007165896968488 1109 72-73 0.6321251482435706 1109 74-75 0.4031048082034907 1109 76 -0.7384790282816596 1110 1 -2.8262815035841378 1110 2 -0.6420228163649213 1110 3 -0.41682159215053716 1110 4 2.1849296901928383 1110 5 1.7995102485234078 1110 6 1.7023475428080772 1110 7 2.2458401613006913 1110 8 1.7977424622161493 1110 9 -1.5777066135618796 1110 10-11 3.39547457294168 1110 12-13 0.10005066205724233 1110 14-15 2.273050505780766 1110 16-17 -2.9624609308819885 1110 18-19 -3.094022132837928 1110 20-21 0.7268537931366836 1110 22-23 1.602072470657994 1110 24-25 -0.6056014526409292 1110 26-27 1.1165582042226738 1110 28-29 -2.7067293817293923 1110 30-31 0.2585712350515017 1110 32-33 1.5921524747182652 1110 34-35 2.636995132060921 1110 36-37 -0.023107192515091413 1110 38-39 1.2239846045767102 1110 40-41 1.7424020789152408 1110 42-43 -0.3710843980580769 1110 44-45 -1.4706021317863396 1110 46-47 1.5978291262830773 1110 48-49 -4.680568316917004 1110 50-51 1.5664717227217224 1110 52-53 -0.6545601876193956 1110 54-55 1.8597884571568741 1110 56-57 0.2428963215147455 1110 58-59 -1.8962033215322727 1110 60-61 -0.867636128491398 1110 62-63 -0.5051969594732704 1110 64-65 0.07352353421432056 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-1.7208265053659773 2117 8 2.014409128882818 2117 9 -4.944373280228547 2117 10-11 -3.4878587603916564 2117 12-13 -1.1499493379427577 2117 14-15 -2.9186161608858967 2117 16-17 -0.08746093088198847 2117 18-19 -1.844022132837928 2117 20-21 -5.58147954019665 2117 22-23 -3.7979275293420045 2117 24-25 -1.5389347859742664 2117 26-27 -2.366775129110657 2117 28-29 -2.2983960483960573 2117 30-31 -2.0997620982818326 2117 32-33 -5.857847525281734 2117 34-35 -5.571338201272415 2117 36-37 -1.2397738591817564 2117 38-39 -4.667682062089954 2117 40-41 -2.882597921084759 2117 42-43 0.9622489352752552 2117 44-45 -2.545602131786339 2117 46-47 -1.1105042070502584 2117 48-49 0.8944316830829955 2117 50-51 -4.166861610611612 2117 52-53 -2.171226854286065 2117 54-55 -0.5568782095097902 2117 56-57 -0.7071036784852573 2117 58-59 -4.6545366548656055 2117 60-61 -7.07596946182473 2117 62-63 -1.3718636261399375 2117 64-65 -2.5931431324523437 2117 66-67 -5.993470126364858 2117 68-69 -0.6849540079803305 2117 70-71 0.5531295641493017 2117 72-73 -5.6371056209871995 2117 74-75 -1.3045874994888145 2117 76 -2.0615559513585815 2118 1 3.09038516308253 2118 2 -2.0920228163649206 2118 3 -0.8834882588172057 2118 4 -4.6984036431404945 2118 5 -8.36715641814326 2118 6 -9.614319123858591 2118 7 -8.120826505365976 2118 8 -0.9855908711171821 2118 9 0.6556267197714511 2118 10-11 1.2121412396083429 2118 12-13 -1.4499493379427584 2118 14-15 -0.5186161608858981 2118 16-17 -4.787460930881988 2118 18-19 1.3559778671620712 2118 20-21 -0.4814795401966485 2118 22-23 -1.4979275293420073 2118 24-25 -4.538934785974266 2118 26-27 0.1332248708893431 2118 28-29 -2.398396048396055 2118 30-31 -6.799762098281834 2118 32-33 -3.0578475252817334 2118 34-35 -0.4713382012724132 2118 36-37 3.0602261408182443 2118 38-39 -3.6676820620899537 2118 40-41 -5.0825979210847585 2118 42-43 -6.437751064724745 2118 44-45 -4.045602131786339 2118 46-47 -1.6105042070502584 2118 48-49 2.8944316830829955 2118 50-51 0.03313838938838387 2118 52-53 2.528773145713938 2118 54-55 -0.756878209509793 2118 56-57 -2.707103678485254 2118 58-59 -3.2545366548656034 2118 60-61 2.6240305381752727 2118 62-63 -3.471863626139939 2118 64-65 3.306856867547655 2118 66-67 5.806529873635139 2118 68-69 -3.084954007980329 2118 70-71 -1.4468704358506947 2118 72-73 -2.737105620987201 2118 74-75 1.1954125005111855 2118 76 4.738444048641416 2119 1 -8.90961483691747 2119 2 -5.892022816364921 2119 3 5.249845074516127 2119 4 6.434929690192838 2119 5 -0.36715641814326005 2119 6 4.452347542808077 2119 7 11.079173494634023 2119 8 4.547742462216149 2119 9 1.5889600531047847 2119 10-11 -8.521192093724988 2119 12-13 -5.983282671276092 2119 14-15 -4.185282827552566 2119 16-17 -3.2541275975486563 2119 18-19 2.155977867162072 2119 20-21 -2.314812873529984 2119 22-23 -10.897927529342006 2119 24-25 -4.605601452640933 2119 26-27 -8.466775129110658 2119 28-29 0.8349372849372791 2119 30-31 -9.533095431615168 2119 32-33 -9.991180858615069 2119 34-35 -7.2380048679390825 2119 36-37 -0.60644052584842 2119 38-39 -4.73434872875662 2119 40-41 3.117402078915241 2119 42-43 0.29558226860859094 2119 44-45 9.821064534880325 2119 46-47 -5.943837540383591 2119 48-49 -2.805568316917004 2119 50-51 -6.100194943944945 2119 52-53 2.9287731457139365 2119 54-55 -2.2235448761764545 2119 56-57 -2.5071036784852545 2119 58-59 0.14546334513439518 2119 60-61 6.2573638715086055 2119 62-63 -2.171863626139938 2119 64-65 -3.2598097991190116 2119 66-67 -1.3268034596981906 2119 68-69 -0.08495400798032904 2119 70-71 5.7531295641493045 2119 72-73 2.5295610456794684 2119 74-75 0.7620791671778484 2119 76 5.671777381974749 >>END_MODULE >>Per sequence quality scores fail #Quality Count 12 10.0 13 67.0 14 175.0 15 290.0 16 341.0 17 389.0 18 446.0 19 407.0 20 354.0 21 263.0 22 179.0 23 108.0 24 76.0 25 67.0 26 48.0 27 28.0 28 30.0 29 24.0 30 30.0 31 25.0 32 19.0 33 43.0 34 40.0 35 48.0 36 99.0 37 104.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.071505958829903 49.485373781148425 8.42361863488624 34.019501625135426 2 3.9622641509433962 27.38544474393531 24.98652291105121 43.66576819407008 3 16.28032345013477 29.946091644204852 23.099730458221025 30.673854447439354 4 5.579514824797844 30.026954177897576 31.347708894878707 33.04582210242587 5 15.929919137466308 30.40431266846361 24.285714285714285 29.380053908355798 6 9.245283018867925 30.808625336927225 10.673854447439354 49.2722371967655 7 7.412398921832884 34.15094339622641 20.997304582210244 37.43935309973046 8 6.4959568733153645 34.285714285714285 14.90566037735849 44.31266846361186 9 10.889487870619947 26.657681940700805 13.53099730458221 48.921832884097036 10-11 8.342318059299192 29.514824797843666 24.353099730458222 37.78975741239892 12-13 7.870619946091645 26.078167115902968 24.460916442048518 41.59029649595688 14-15 12.129380053908356 30.296495956873315 19.514824797843666 38.05929919137466 16-17 16.88679245283019 29.770889487870623 19.11051212938005 34.23180592991914 18-19 13.315363881401618 28.517520215633425 18.08625336927224 40.080862533692724 20-21 11.159029649595688 28.342318059299192 19.043126684636118 41.455525606469 22-23 17.02156334231806 29.8921832884097 15.849056603773585 37.23719676549865 24-25 14.784366576819409 33.463611859838274 14.514824797843664 37.23719676549865 26-27 13.11320754716981 34.83827493261455 19.2722371967655 32.77628032345014 28-29 15.525606469002696 30.026954177897576 23.477088948787063 30.97035040431267 30-31 14.824797843665769 28.84097035040431 25.78167115902965 30.552560646900268 32-33 10.148247978436657 35.60646900269542 22.66846361185984 31.576819407008088 34-35 10.646900269541778 35.4177897574124 19.757412398921833 34.17789757412399 36-37 12.641509433962264 29.514824797843666 23.49056603773585 34.35309973045822 38-39 13.49056603773585 24.82479784366577 27.008086253369274 34.67654986522911 40-41 10.700808625336927 26.8733153638814 25.45822102425876 36.967654986522916 42-43 7.021563342318059 24.002695417789756 29.420485175202156 39.55525606469003 44-45 7.722371967654987 26.442048517520217 31.73854447439353 34.097035040431265 46-47 8.153638814016173 30.70080862533693 26.37466307277628 34.77088948787062 48-49 8.315363881401618 33.34231805929919 21.388140161725065 36.95417789757413 50-51 8.018867924528301 31.75202156334232 20.350404312668463 39.878706199460915 52-53 7.3854447439353095 32.2911051212938 21.212938005390836 39.110512129380055 54-55 6.940700808625337 30.49865229110512 20.59299191374663 41.96765498652291 56-57 11.347708894878707 30.943396226415093 19.19137466307278 38.517520215633425 58-59 10.592991913746632 29.973045822102424 18.234501347708896 41.19946091644205 60-61 8.059299191374663 27.66846361185984 19.541778975741238 44.730458221024264 62-63 10.943396226415095 28.140161725067387 18.62533692722372 42.291105121293796 64-65 11.01078167115903 29.90566037735849 14.743935309973047 44.339622641509436 66-67 9.460916442048518 36.46900269541779 12.668463611859837 41.40161725067386 68-69 12.129380053908356 35.21563342318059 12.843665768194072 39.81132075471698 70-71 13.814016172506738 36.57681940700809 12.318059299191376 37.291105121293796 72-73 9.420485175202156 35.471698113207545 11.940700808625337 43.16711590296496 74-75 8.315363881401618 37.924528301886795 12.776280323450134 40.98382749326145 76 7.061994609164421 31.266846361185983 13.07277628032345 48.59838274932615 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 0.5 15 0.5 16 0.5 17 0.0 18 1.0 19 2.0 20 4.5 21 7.0 22 6.5 23 8.5 24 9.5 25 8.0 26 9.0 27 11.0 28 18.5 29 25.0 30 23.0 31 30.0 32 40.0 33 41.0 34 41.5 35 58.5 36 77.5 37 80.0 38 88.5 39 104.5 40 121.5 41 144.0 42 157.0 43 164.0 44 188.0 45 211.0 46 217.0 47 217.0 48 221.0 49 228.0 50 231.0 51 224.5 52 205.5 53 198.5 54 204.0 55 184.5 56 156.0 57 136.0 58 125.0 59 116.0 60 100.5 61 86.5 62 79.0 63 62.5 64 48.5 65 43.0 66 25.5 67 16.0 68 18.0 69 16.0 70 11.0 71 10.0 72 8.5 73 5.0 74 4.0 75 5.0 76 5.5 77 3.5 78 1.5 79 2.0 80 2.5 81 3.0 82 1.5 83 0.0 84 0.0 85 0.0 86 0.5 87 1.0 88 1.0 89 1.0 90 1.5 91 2.0 92 2.0 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48517520215633425 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 3710.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.1967654986523 #Duplication Level Percentage of deduplicated Percentage of total 1 98.0865224625624 95.33692722371968 2 1.2201885745978924 2.371967654986523 3 0.4714364947310039 1.3746630727762803 4 0.16638935108153077 0.646900269541779 5 0.055463117027176934 0.2695417789757413 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATC 5 0.13477088948787064 No Hit CTTATACACATCTGACGCTGCCGACGAGGCAACCTGTGTAGATCTCGGTG 5 0.13477088948787064 No Hit CTTATACACATCTGACGCCTGTCTCTTATACACATCTGACGCTGCCGACG 4 0.1078167115902965 No Hit CTCTTCCTTTGGTCCTATTTCTGTTATATAAGACAATATAATCATTTATT 4 0.1078167115902965 No Hit GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG 4 0.1078167115902965 No Hit CATCAAAGGCCTTTTAAAATTAGAAGAGGATAAGGGAAATTCTAAGACTC 4 0.1078167115902965 No Hit ATAATATAATCATTGGGCATGAAATAGATGCAACTTAAGGCTTTTCAATA 4 0.1078167115902965 No Hit CATCCTATTCATTCTTTATCTCTGGGCAACCATGACTAGCTAATACAGTC 4 0.1078167115902965 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.16172506738544473 0.0 2 0.0 0.0 0.0 0.16172506738544473 0.0 3 0.0 0.0 0.0 0.16172506738544473 0.0 4 0.0 0.0 0.0 0.16172506738544473 0.0 5 0.0 0.0 0.0 0.18867924528301888 0.0 6 0.0 0.0 0.0 0.18867924528301888 0.0 7 0.0 0.0 0.0 0.18867924528301888 0.0 8 0.0 0.0 0.0 0.18867924528301888 0.0 9 0.0 0.0 0.0 0.18867924528301888 0.0 10 0.0 0.0 0.0 0.18867924528301888 0.0 11 0.0 0.0 0.0 0.24258760107816713 0.0 12 0.0 0.0 0.0 0.24258760107816713 0.0 13 0.0 0.0 0.0 0.24258760107816713 0.0 14 0.0 0.0 0.0 0.2695417789757412 0.0 15 0.0 0.0 0.0 0.2695417789757412 0.0 16 0.0 0.0 0.0 0.29649595687331537 0.0 17 0.0 0.0 0.0 0.29649595687331537 0.0 18 0.0 0.0 0.0 0.40431266846361186 0.0 19 0.0 0.0 0.0 0.7008086253369272 0.0 20 0.0 0.0 0.0 0.7277628032345014 0.0 21 0.0 0.0 0.0 0.7547169811320755 0.0 22 0.0 0.0 0.0 0.8355795148247979 0.0 23 0.0 0.0 0.0 0.8355795148247979 0.0 24 0.0 0.0 0.0 0.8355795148247979 0.0 25 0.0 0.0 0.0 0.862533692722372 0.0 26 0.0 0.0 0.0 0.862533692722372 0.0 27 0.0 0.0 0.0 0.9433962264150944 0.0 28 0.0 0.0 0.0 0.9433962264150944 0.0 29 0.0 0.0 0.0 0.9703504043126685 0.0 30 0.0 0.0 0.0 0.9973045822102425 0.0 31 0.0 0.0 0.0 1.0242587601078168 0.0 32 0.0 0.0 0.0 1.105121293800539 0.0 33 0.0 0.0 0.0 1.1590296495956873 0.0 34 0.0 0.0 0.0 1.1859838274932615 0.0 35 0.0 0.0 0.0 1.266846361185984 0.0 36 0.0 0.0 0.0 1.5902964959568733 0.0 37 0.0 0.0 0.0 1.8059299191374663 0.0 38 0.0 0.0 0.0 2.1024258760107815 0.0 39 0.0 0.0 0.0 2.533692722371968 0.0 40 0.0 0.0 0.0 2.9110512129380055 0.0 41 0.0 0.0 0.0 2.9919137466307277 0.0 42 0.0 0.0 0.0 3.288409703504043 0.0 43 0.0 0.0 0.0 3.477088948787062 0.0 44 0.0 0.0 0.0 3.8005390835579513 0.0 45 0.0 0.0 0.0 4.123989218328841 0.0 46 0.0 0.0 0.0 4.474393530997305 0.0 47 0.0 0.0 0.0 4.636118598382749 0.0 48 0.0 0.0 0.0 4.8517520215633425 0.0 49 0.0 0.0 0.0 4.959568733153639 0.0 50 0.0 0.0 0.0 5.1482479784366575 0.0 51 0.0 0.0 0.0 5.283018867924528 0.0 52 0.0 0.0 0.0 5.336927223719677 0.0 53 0.0 0.0 0.0 5.471698113207547 0.0 54 0.0 0.0 0.0 5.55256064690027 0.0 55 0.0 0.0 0.0 5.741239892183288 0.0 56 0.0 0.0 0.0 5.9568733153638815 0.0 57 0.0 0.0 0.0 6.1455525606469 0.0 58 0.0 0.0 0.0 6.172506738544475 0.0 59 0.0 0.0 0.0 6.2533692722371965 0.0 60 0.0 0.0 0.0 6.388140161725067 0.0 61 0.0 0.0 0.0 6.415094339622642 0.0 62 0.0 0.0 0.0 6.549865229110512 0.0 63 0.0 0.0 0.0 6.657681940700809 0.0 64 0.0 0.0 0.0 6.846361185983827 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCACGC 20 0.006419566 52.5 63 >>END_MODULE