FastQCFastQC Report
Wed 25 May 2016
SRR1780592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3710
Sequences flagged as poor quality0
Sequence length76
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT91224.582210242587603No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT56115.121293800539085TruSeq Adapter, Index 2 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG952.560646900269542No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC641.725067385444744No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC641.725067385444744No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATATCGTATGCCGT551.482479784366577No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT481.293800539083558No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC421.1320754716981132No Hit
TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT391.051212938005391No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT290.7816711590296497No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT210.5660377358490566No Hit
CTTATACACATCTCCGAGCCCACGCGACGAGCACGTATCTCGTATGCCGT180.48517520215633425No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATATCGTATGCCGTCTT170.4582210242587601No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC170.4582210242587601No Hit
ACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCT160.431266846361186RNA PCR Primer, Index 2 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGCCGAGCACGTATCTCGTATGCCGT150.40431266846361186No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGTCTT140.37735849056603776No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATATCGTATGCCG130.3504043126684636No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGTCTT130.3504043126684636No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGT120.3234501347708895No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCG120.3234501347708895No Hit
ATACACATCTCCGAGCCCACGAGAAGAGCACGTATCTCGTATGCCGTCTT110.29649595687331537Illumina PCR Primer Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT110.29649595687331537No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT110.29649595687331537No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTCTCTCGTATGCCGT110.29649595687331537No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTAGTATGCCGT100.2695417789757413No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCG100.2695417789757413No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT90.24258760107816713No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC90.24258760107816713No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCC90.24258760107816713No Hit
ATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGTCTT80.215633423180593TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCACGTATGCCGT80.215633423180593No Hit
ATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTGCTT80.215633423180593TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCCCGTATGCCGT80.215633423180593No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT70.18867924528301888No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAGCTCGTATGCCGT70.18867924528301888No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCCCGTATCTCGTATGCCGT70.18867924528301888No Hit
ATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGTCTT70.18867924528301888Illumina PCR Primer Index 2 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG60.16172506738544476No Hit
CATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTGCT60.16172506738544476TruSeq Adapter, Index 2 (96% over 26bp)
CTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTTCTGCTTGA60.16172506738544476RNA PCR Primer, Index 2 (96% over 28bp)
CTTATACACATCTCCGAGCCCAAGAGACGAGCACGTATCTCGTATGCCGT60.16172506738544476No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGCATCTCGTATGCCGT60.16172506738544476No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGA50.13477088948787064No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATATCGTATGCC50.13477088948787064No Hit
CTTATACACATATCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT50.13477088948787064No Hit
ATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGTCTT50.13477088948787064TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTCTGCCGT50.13477088948787064No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTAGCTCGTATGCCGTCTT50.13477088948787064No Hit
CTTATACACATCTCCGAGCACACGAGACGAGCACGTATCTCGTATGCCGT50.13477088948787064No Hit
CTTATAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG50.13477088948787064No Hit
TGAGAGAACAGCAAATAAATGATTATATTGTCTTATATAACAGAAATAGG50.13477088948787064No Hit
CTTATCACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC50.13477088948787064No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCC50.13477088948787064No Hit
CTTATACACATCTACGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT50.13477088948787064No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTTTCTCGTATGCCGT50.13477088948787064No Hit
TCTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT50.13477088948787064No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAAATCGTATGCCGT50.13477088948787064No Hit
TTGTATTAAGTTCAAAATCAGTGATGCATGTTTAATATTATATGCCTGTC40.1078167115902965No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG40.1078167115902965No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT40.1078167115902965No Hit
CTTAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC40.1078167115902965No Hit
GAATGGAATCGAAGAGAATCTTCGAACGGACCCGAATGGAATCATCTAAT40.1078167115902965No Hit
TATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGTCT40.1078167115902965No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTA40.1078167115902965No Hit
GATCTGAGAAAAATATGCAGAAATTTCAACCCCTGATTCATGTTGTGAAA40.1078167115902965No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATTTCGTATGCCGT40.1078167115902965No Hit
CCTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT40.1078167115902965No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAG40.1078167115902965No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGTACGTATCTCGTATGCCGT40.1078167115902965No Hit
CTTATACACATCTCCGAGCCCACGTGACGAGCACGTATCTCGTATGCCGT40.1078167115902965No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGTC40.1078167115902965No Hit
TAAATCACTAGCAACTCAATCGCCACACCCTTCATGAAAGTTATTTTGTG40.1078167115902965No Hit
CTTATTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG40.1078167115902965No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTCTGCCGTCTT40.1078167115902965No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG40.1078167115902965No Hit
CTTATACACATCTCCGCGCCCACGAGACGAGCACGTATCTCGTATGCCGT40.1078167115902965No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGTCTT40.1078167115902965No Hit
ATTCTTGGGTTATCCCCTAACAAAGCTTTATTGAAAAGCCTTAAGTTGCA40.1078167115902965No Hit
GAGTGGGATGATTGTGACCCTGAATCCCTACAGAGAAAGAGAGTCTGTCT40.1078167115902965No Hit
CTTATACACATCTCCGAGCCCCCGAGACGAGCACGTATCTCGTATGCCGT40.1078167115902965No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTA252.3205557E-670.0000138
GTATGAC150.002053246170.0000142
CTATACA252.3205557E-670.000011
ACGTCTT150.002053246170.0000147
ATGACGT150.002053246170.0000144
ACGTAAC252.3205557E-670.0000133
TATGACG150.002053246170.0000143
AACTCGT252.3205557E-670.0000137
TAACTCG252.3205557E-670.0000136
GACGTCT150.002053246170.0000146
CGTATGA150.002053246170.0000141
CGTAACT252.3205557E-670.0000134
AAAATAA150.002053246170.0000169
GTAACTC252.3205557E-670.0000135
TGACGTC150.002053246170.0000145
CACGTAA252.0921287E-456.00000432
TTATACA2550.054.9019622
CTTATAC2550.054.9019621
AAAAATA200.00641956652.568
TATACAC2800.050.03
Produced by FastQC (version 0.11.5)