FastQCFastQC Report
Wed 25 May 2016
SRR1780591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50836
Sequences flagged as poor quality0
Sequence length50
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATC850.16720434337870801No Hit
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG600.11802659532614683No Hit
GCGTCAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTGCCGACG570.1121252655598395No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC560.11015815563773704No Hit
GATGCTGTCTCTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTA530.10425682587142969No Hit
CTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACGA510.1003226060272248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA404.0209488E-738.5008621
GACCTAA250.002320127635.2355423
GGTGGTC250.002354097835.13149644
AAAATGA250.002354097835.13149632
TTTTATA250.002354097835.13149642
CCTCTGA250.002354097835.1314966
CCCGAGG250.002354097835.1314963
TTTACAC451.0270142E-634.155622
AGGTGAC353.1561352E-431.46029935
CGCTGTC300.00563851529.39196417
GAGGATG300.005665851329.36294722
ATTATTG300.005665851329.36294740
TCTCGGG300.00574848829.27624743
TCCACAT300.00574848829.2762472
GTGTAAA300.00574848829.27624730
CAAGCTT300.00574848829.2762476
CCAAGAG300.00574848829.2762472
AGAGGGC300.00574848829.2762475
GCAGTGT554.9133323E-627.94550930
ACGAAGT655.5481905E-727.18476921