FastQCFastQC Report
Wed 25 May 2016
SRR1780591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50836
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT2515249.47674876072075No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT602611.853804390589346RNA PCR Primer, Index 34 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG33176.524903611613817No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC28435.592493508537257No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC7531.4812337713431427RNA PCR Primer, Index 34 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT6301.2392792509245416RNA PCR Primer, Index 34 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC5431.0681406877016286No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC2970.5842316468644267No Hit
CATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTTCTGCT2280.448501062239358RNA PCR Primer, Index 34 (96% over 28bp)
ATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTTCTGCTT2130.41899441340782123Illumina PCR Primer Index 3 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT1870.3678495554331576No Hit
ACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTTCT1650.3245731371469038RNA PCR Primer, Index 34 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC1400.2753953890943426No Hit
TCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTTCTGCTTG1240.24392163034070344RNA PCR Primer, Index 34 (96% over 30bp)
CTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG1110.21834920135337163No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG1010.19867810213234716No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTA1000.1967109922102447No Hit
CTTATAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG800.15736879376819576No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGATCTCGTATGCCGTC760.14950035407978599No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGC730.14359902431347865No Hit
CTTATTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG620.12196081517035172No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG610.11999370524824926No Hit
CTTAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC610.11999370524824926RNA PCR Primer, Index 34 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGTCTT570.1121252655598395No Hit
CTTATCACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC560.11015815563773704No Hit
CATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCT550.10819104571563459No Hit
CGTCAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA540.10622393579353213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG207.7855506E-444.00000444
TCTTTAT302.4800866E-644.0000041
CTTTACA850.043.9999961
TTTACAC850.043.9999962
CTATACA850.041.4117621
TTACACA950.039.368423
CCGTCTT6900.039.21739244
CTTTATA357.1537543E-637.7142872
TTTATAC357.1537543E-637.7142873
GCCGTCT7750.034.9161343
CTTATAC34550.033.8118671
TTATACA35000.033.3771442
TATACAC36450.032.1097373
ATGCCGT36300.032.044
TATGCCG40050.028.89388343
CTCTTAT4700.028.0851081
ATACACA42450.027.4676074
TGCCGTC9950.027.19598242
TACACAT43200.027.0416665
ACACATC43400.026.9677416
Produced by FastQC (version 0.11.5)