FastQCFastQC Report
Wed 25 May 2016
SRR1780590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28172
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGAGA150.002202808969.99644550
TCTGGTG150.002202808969.99644547
GGTCTGG150.002202808969.99644545
GTCTGGT150.002202808969.99644546
GAATGGC150.002202808969.99644564
CTGGTGA150.002202808969.99644548
TGGGGCT150.002202808969.99644521
ACGGTTG150.002202808969.99644544
TAAGGTG150.002202808969.9964455
TATGGCT150.002202808969.9964454
CGCCTAG252.351645E-455.99715836
GGAGTTT252.351645E-455.99715859
TTGTCTG200.006883529552.49733426
GTTTGGG200.006883529552.49733418
GTCATTA200.006883529552.49733468
GGGTCGC200.006883529552.49733432
TGTCTGG200.006883529552.49733427
AGGGTAT200.006883529552.49733417
TAATGTC200.006883529552.49733464
ATAATTG200.006883529552.49733422
Produced by FastQC (version 0.11.5)