##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780590_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28172 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.068436745704954 34.0 27.0 34.0 12.0 34.0 2 30.86923186142269 34.0 32.0 34.0 21.0 34.0 3 31.082599744427093 34.0 33.0 34.0 23.0 34.0 4 31.29635808604288 34.0 33.0 34.0 23.0 34.0 5 30.812579866534147 34.0 32.0 34.0 21.0 34.0 6 33.81822376828056 37.0 34.0 38.0 21.0 38.0 7 33.084374556297035 37.0 34.0 38.0 21.0 38.0 8 31.973626295612664 37.0 31.0 38.0 11.0 38.0 9 33.28865540252733 37.0 34.0 38.0 21.0 38.0 10-11 33.46613658952151 37.0 34.0 38.0 21.0 38.0 12-13 33.70701405651001 37.0 34.0 38.0 21.5 38.0 14-15 33.60359576884851 37.0 34.0 38.0 21.0 38.0 16-17 33.46879880732642 37.0 34.0 38.0 21.0 38.0 18-19 33.4848076103933 37.0 34.0 38.0 21.0 38.0 20-21 33.50937100667329 37.0 34.0 38.0 21.0 38.0 22-23 33.42842183728525 37.0 34.0 38.0 21.0 38.0 24-25 33.911720857589096 38.0 34.5 38.0 21.5 38.0 26-27 33.8961735056084 38.0 34.0 38.0 21.0 38.0 28-29 34.06639571205452 38.0 35.0 38.0 22.5 38.0 30-31 34.00285744711061 38.0 34.5 38.0 21.5 38.0 32-33 33.534040891665484 37.5 34.0 38.0 21.0 38.0 34-35 33.72825855459321 37.0 34.0 38.0 21.0 38.0 36-37 33.7437881584552 37.0 34.0 38.0 21.0 38.0 38-39 34.11646315490558 38.0 35.0 38.0 22.5 38.0 40-41 33.65737256850774 37.5 34.0 38.0 21.0 38.0 42-43 33.82605068862701 38.0 34.0 38.0 21.0 38.0 44-45 32.860819253159164 37.0 32.5 38.0 16.0 38.0 46-47 33.65274030952719 37.0 34.0 38.0 21.5 38.0 48-49 33.491267925599885 37.0 34.0 38.0 21.0 38.0 50-51 33.20353542524492 37.0 34.0 38.0 21.0 38.0 52-53 33.17760542382507 37.0 33.5 38.0 21.0 38.0 54-55 32.9880910123527 37.0 33.0 38.0 21.0 38.0 56-57 32.99282976004544 37.0 32.5 38.0 16.0 38.0 58-59 32.85764233991197 37.0 32.5 38.0 16.0 38.0 60-61 32.96517819111174 37.0 32.5 38.0 16.0 38.0 62-63 32.94902740309527 37.0 32.5 38.0 19.0 38.0 64-65 33.00933551043589 37.0 32.5 38.0 19.5 38.0 66-67 33.022717591935255 37.0 32.5 38.0 19.5 38.0 68-69 32.35329405083061 37.0 31.0 38.0 15.0 38.0 70-71 32.540270481328974 37.0 31.0 38.0 15.0 38.0 72-73 32.67428652562828 37.0 31.5 38.0 15.0 38.0 74-75 32.183906005963365 37.0 30.5 38.0 15.0 38.0 76 31.548913815135595 36.0 28.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.1262103506201058 1101 2 -0.4513728686122569 1101 3 0.28811004905923454 1101 4 -2.187949514900005 1101 5 0.015964530265677013 1101 6 -0.02112796825284846 1101 7 -0.5629399265230859 1101 8 0.29403490353135453 1101 9 0.4992953625774703 1101 10-11 0.15227215760869228 1101 12-13 0.05670331646395965 1101 14-15 0.09605682222845502 1101 16-17 -0.9435810176744326 1101 18-19 0.7254804698649551 1101 20-21 0.029265897634246585 1101 22-23 0.47463881028252786 1101 24-25 0.0814219128806144 1101 26-27 -0.780888526302931 1101 28-29 0.8002928247712333 1101 30-31 -0.11211883685433577 1101 32-33 1.3916651185384694 1101 34-35 0.13342929478076115 1101 36-37 1.2450957156713827 1101 38-39 0.9296631433128866 1101 40-41 1.2658797429660993 1101 42-43 1.15550609894197 1101 44-45 1.5437857052679647 1101 46-47 1.1999032838924677 1101 48-49 0.768547327637755 1101 50-51 0.4586379114851269 1101 52-53 0.6294191980403312 1101 54-55 0.6205440262890605 1101 56-57 0.4445425711045914 1101 58-59 0.16142992666885192 1101 60-61 0.4117024698119067 1101 62-63 -0.5757607637890274 1101 64-65 0.20558978159918695 1101 66-67 0.7614368525749597 1101 68-69 0.902088107241628 1101 70-71 1.361501741047629 1101 72-73 0.8460061071462128 1101 74-75 -0.451650403795572 1101 76 -1.512279913048424 1102 1 0.7813165311003267 1102 2 -0.12363093312838558 1102 3 0.7026261780914957 1102 4 0.14710424854085602 1102 5 0.9366096915560007 1102 6 0.9985494511019937 1102 7 1.1970600734769121 1102 8 2.2958628605206 1102 9 0.9989727819323093 1102 10-11 -0.10159881013323968 1102 12-13 -2.7713611996650727 1102 14-15 -1.7262012422876722 1102 16-17 -1.8301939209002427 1102 18-19 0.30962025481120037 1102 20-21 1.4315239621503792 1102 22-23 -0.014178394018543372 1102 24-25 0.6958842784720076 1102 26-27 -4.930404655335188 1102 28-29 -0.712395347271773 1102 30-31 0.6944940663714689 1102 32-33 -6.031184343827121 1102 34-35 0.006762628114096003 1102 36-37 0.3582677586821319 1102 38-39 0.3342330357860064 1102 40-41 0.9302345816757835 1102 42-43 1.2874415828129386 1102 44-45 -5.03094547752773 1102 46-47 -0.26439779137634645 1102 48-49 0.8624182953796904 1102 50-51 -3.277491120772936 1102 52-53 -0.7874087589489136 1102 54-55 2.003070908009498 1102 56-57 -1.0660488267448684 1102 58-59 1.5917525073140126 1102 60-61 0.7276702117473945 1102 62-63 -3.792803774541717 1102 64-65 0.5356973084809056 1102 66-67 0.45670566977926796 1102 68-69 -7.681083935769124 1102 70-71 0.609566257176656 1102 72-73 0.9909523437053487 1102 74-75 -6.168585887666541 1102 76 -1.2916347517580995 1103 1 1.9896498644336589 1103 2 1.2080357335382814 1103 3 1.035959511424828 1103 4 1.0354375818741879 1103 5 2.204943024889335 1103 6 -0.46978388223134004 1103 7 1.7120600734769127 1103 8 2.4808628605206025 1103 9 1.2589727819323073 1103 10-11 2.6684011898667563 1103 12-13 0.7444721336682605 1103 14-15 1.1054654243789983 1103 16-17 1.6964727457664281 1103 18-19 1.3762869214778632 1103 20-21 0.9948572954837118 1103 22-23 1.309988272648127 1103 24-25 1.9100509451386714 1103 26-27 1.5629286779981442 1103 28-29 1.5226046527282264 1103 30-31 1.410327399704805 1103 32-33 -3.5728510104937854 1103 34-35 -0.166570705219236 1103 36-37 0.9582677586821404 1103 38-39 0.9383997024526707 1103 40-41 2.2935679150091133 1103 42-43 2.0016082494796095 1103 44-45 -4.15594547752773 1103 46-47 1.0164355419569873 1103 48-49 0.9357516287130281 1103 50-51 1.36750887922706 1103 52-53 0.650924574384419 1103 54-55 0.869737574676158 1103 56-57 2.249784506588462 1103 58-59 1.8225858406473492 1103 60-61 2.0243368784140614 1103 62-63 1.356362892124956 1103 64-65 0.9815306418142384 1103 66-67 1.7158723364459263 1103 68-69 -1.0402506024357905 1103 70-71 1.7153995905099926 1103 72-73 1.6851190103720164 1103 74-75 0.6939141123334629 1103 76 1.38503191490857 1104 1 -0.8957326492275435 1104 2 0.7950575914617772 1104 3 0.9263966698947748 1104 4 0.9943173632949538 1104 5 1.2851342817199338 1104 6 0.7213363363478962 1104 7 3.076404335771997 1104 8 2.2206169588812585 1104 9 0.3893006507847687 1104 10-11 0.582335616096259 1104 12-13 0.14290109541688878 1104 14-15 1.0801922003352757 1104 16-17 0.4613634561489377 1104 18-19 2.2928169761226727 1104 20-21 2.5943108473962724 1104 22-23 2.123854392866704 1104 24-25 1.7505564096195485 1104 26-27 1.8267264922057933 1104 28-29 1.051703013383964 1104 30-31 0.08129734505999409 1104 32-33 0.44463532830402386 1104 34-35 1.5610249231960651 1104 36-37 1.2529398898296762 1104 38-39 1.627265822671255 1104 40-41 2.4680214669216767 1104 42-43 1.8441628942883455 1104 44-45 -3.2129126906424865 1104 46-47 0.823471061082671 1104 48-49 1.871270754396086 1104 50-51 2.409721993981165 1104 52-53 1.1331650115428857 1104 54-55 1.0436446785012947 1104 56-57 1.6117380585010253 1104 58-59 2.2755229991172925 1104 60-61 1.4752931625670627 1104 62-63 2.096458520540253 1104 64-65 1.5619268166776266 1104 66-67 2.115394194369429 1104 68-69 -3.2573954111789583 1104 70-71 0.4146482243897758 1104 72-73 2.2334113600987884 1104 74-75 -0.03784818274850821 1104 76 1.2486931170943585 1105 1 -1.921909275351286 1105 2 -0.233630933128385 1105 3 -0.08285769287624589 1105 4 0.6238784420892429 1105 5 0.6208032399430969 1105 6 0.9627429994890875 1105 7 0.8080278154123945 1105 8 0.35586286052060245 1105 9 1.5638114916097265 1105 10-11 1.1845302221248204 1105 12-13 -0.47555474805217557 1105 14-15 0.7170245641639426 1105 16-17 1.4798060790997596 1105 18-19 2.038652512875707 1105 20-21 0.5816852524729512 1105 22-23 1.1614667672717758 1105 24-25 1.5437875042784555 1105 26-27 0.9045953446648127 1105 28-29 0.15244336240564849 1105 30-31 0.8394940663714721 1105 32-33 1.3083317852051337 1105 34-35 1.3457948861786093 1105 36-37 0.9453645328756863 1105 38-39 1.6608459390118142 1105 40-41 1.1731378074822274 1105 42-43 1.9619577118451943 1105 44-45 2.780344845052909 1105 46-47 1.7818925312042921 1105 48-49 1.5427408760248582 1105 50-51 0.8054121050335112 1105 52-53 0.4627525313736598 1105 54-55 0.905490262848204 1105 56-57 1.0950802055131916 1105 58-59 0.94368799118498 1105 60-61 1.218154082715138 1105 62-63 1.8933252577163486 1105 64-65 1.6477940826744586 1105 66-67 1.7036411536502314 1105 68-69 2.4840773545534525 1105 70-71 2.385695289434725 1105 72-73 0.6188555695117977 1105 74-75 -0.24197298444073212 1105 76 -0.32679604208068014 1106 1 0.07935574678660018 1106 2 0.4477416158912213 1106 3 0.5290967663267878 1106 4 -0.18858202596894813 1106 5 0.5671979268501168 1106 6 0.6003141569843464 1106 7 0.9272561519082885 1106 8 1.0470393311088344 1106 9 1.3462276838930975 1106 10-11 0.9674207977098916 1106 12-13 0.5682466434721789 1106 14-15 1.2549752283005589 1106 16-17 1.447943334001721 1106 18-19 1.0003065293210014 1106 20-21 0.889955334699394 1106 22-23 1.5244490569618492 1106 24-25 2.6122568274916134 1106 26-27 2.3163600505471607 1106 28-29 0.07211445664979266 1106 30-31 0.9865528899008851 1106 32-33 2.150678401270916 1106 34-35 1.4128410594866452 1106 36-37 1.8009148175056637 1106 38-39 2.219036957354632 1106 40-41 2.1607247777542113 1106 42-43 2.0690102102639187 1106 44-45 2.1043486401193263 1106 46-47 2.873053189015806 1106 48-49 2.370065354203227 1106 50-51 1.8493716243251015 1106 52-53 1.4646500645804892 1106 54-55 0.9976787511467435 1106 56-57 2.6818923497257146 1106 58-59 1.0105760367257801 1106 60-61 1.6111015842964136 1106 62-63 2.30121583330142 1106 64-65 2.2778541712260036 1106 66-67 2.287686061936128 1106 68-69 2.9148964563877406 1106 70-71 1.9872133160001901 1106 72-73 1.9642856770386778 1106 74-75 0.7230807790001244 1106 76 0.4801299541242514 1107 1 0.5488840986678944 1107 2 0.5343870848896337 1107 3 1.0102838357491528 1107 4 1.000077221513827 1107 5 0.8308439257902336 1107 6 0.48134224389478675 1107 7 1.1667898032066404 1107 8 2.4887457434034843 1107 9 1.6323962053557324 1107 10-11 1.8981309195964826 1107 12-13 2.0644045661006913 1107 14-15 2.117852811766376 1107 16-17 2.1678691421628216 1107 18-19 2.762097732288673 1107 20-21 2.111073511699928 1107 22-23 1.1427585429183864 1107 24-25 0.5930464406341684 1107 26-27 1.8077484978179683 1107 28-29 1.7232803284039022 1107 30-31 1.3349895618669692 1107 32-33 2.6289507913080143 1107 34-35 2.2865824479339167 1107 36-37 2.3206551460695266 1107 38-39 2.7542780808310496 1107 40-41 2.5320814285226234 1107 42-43 2.451495636866987 1107 44-45 1.884820288238032 1107 46-47 1.9190256320470738 1107 48-49 2.0452110881724863 1107 50-51 3.0100764467946277 1107 52-53 2.4256993491591956 1107 54-55 1.7235664035049822 1107 56-57 2.056428650732606 1107 58-59 2.4157164712779746 1107 60-61 2.6295170585942387 1107 62-63 1.7206646939267571 1107 64-65 1.3384000111836087 1107 66-67 2.359228192301785 1107 68-69 3.276979127293945 1107 70-71 1.8492959869063874 1107 72-73 1.8787000914531014 1107 74-75 2.1442519501712987 1107 76 1.6629598428364964 1108 1 0.6871988840415035 1108 2 1.3497024002049471 1108 3 1.1369399035816912 1108 4 0.8898493465800712 1108 5 1.0868057699873717 1108 6 1.8552161177686557 1108 7 2.1331385048494624 1108 8 2.1548824683637378 1108 9 2.238384546638194 1108 10-11 1.3938913859451887 1108 12-13 1.465305467001599 1108 14-15 2.034386993006443 1108 16-17 0.9724531379232886 1108 18-19 1.054228097948446 1108 20-21 1.948778864111162 1108 22-23 0.9215078804912622 1108 24-25 2.0289234941582777 1108 26-27 0.9340071093706968 1108 28-29 1.9348595546890124 1108 30-31 1.854199948724414 1108 32-33 1.5134234993101288 1108 34-35 1.6481351771337032 1108 36-37 1.4871893273095864 1108 38-39 2.263154604413458 1108 40-41 1.7391561503032236 1108 42-43 1.618029818107054 1108 44-45 2.1680741303154036 1108 46-47 2.005406130192277 1108 48-49 2.149477118909097 1108 50-51 0.6042735851094108 1108 52-53 0.5675912410510833 1108 54-55 1.7721885550683183 1108 56-57 1.980911957568857 1108 58-59 0.42234074260812804 1108 60-61 2.042474133316027 1108 62-63 2.6492550489876976 1108 64-65 2.253344367304436 1108 66-67 1.7484703756616113 1108 68-69 3.2776415544269533 1108 70-71 3.163683904235487 1108 72-73 2.2535013633131946 1108 74-75 2.4632768574314987 1108 76 2.127188777653668 1109 1 -1.2964612466774526 1109 2 -0.07251982201727358 1109 3 0.6970706225359393 1109 4 0.3493264707630779 1109 5 0.3854985804448887 1109 6 -0.6003394377869 1109 7 -0.4407177043008659 1109 8 0.41697397163171246 1109 9 0.008972781932307328 1109 10-11 1.0739567454223078 1109 12-13 0.19308324477937333 1109 14-15 0.24713209104566403 1109 16-17 -0.22297169867801614 1109 18-19 0.8568424770334175 1109 20-21 1.422635073261489 1109 22-23 1.2113771615370155 1109 24-25 1.4086620562497814 1109 26-27 0.826817566887037 1109 28-29 -0.10795090282732644 1109 30-31 -0.3105059336285265 1109 32-33 1.2382601006173175 1109 34-35 1.1667626281140997 1109 36-37 0.477712203126579 1109 38-39 1.0981219246748921 1109 40-41 1.5296790261202275 1109 42-43 1.8807749161462723 1109 44-45 0.9023878558055998 1109 46-47 0.31226887529031444 1109 48-49 -0.9531372601758648 1109 50-51 1.2202866570048343 1109 52-53 1.2175912410510819 1109 54-55 0.9502931302317137 1109 56-57 0.984506728810679 1109 58-59 0.7017525073140121 1109 60-61 0.7604479895251686 1109 62-63 0.5133073365693974 1109 64-65 0.5356973084809127 1109 66-67 0.5755945586681577 1109 68-69 0.7694716197864331 1109 70-71 0.5417884793988783 1109 72-73 -0.13571432296131647 1109 74-75 1.2230807790001244 1109 76 0.27947635935301207 1110 1 -0.6734453736615791 1110 2 -0.5193452188426697 1110 3 0.07881665428196882 1110 4 0.3366280580646652 1110 5 0.37041921536552636 1110 6 -0.2638315012789576 1110 7 -0.5391304027135604 1110 8 0.8677676224253617 1110 9 -1.9338843609248322 1110 10-11 -1.5994559529903896 1110 12-13 -0.3442183425222112 1110 14-15 -0.2278679089543445 1110 16-17 0.18813941243308818 1110 18-19 1.0703345405254865 1110 20-21 -0.1587141330877202 1110 22-23 -0.2167974416375955 1110 24-25 -0.009591912004182745 1110 26-27 -0.8692141791447128 1110 28-29 0.1904617955853638 1110 30-31 -1.112886886009477 1110 32-33 0.6783394656966877 1110 34-35 0.11557215192361525 1110 36-37 0.22493442534880614 1110 38-39 0.14970922626219618 1110 40-41 -0.3088130373718414 1110 42-43 0.46887015424150746 1110 44-45 1.5714354748532173 1110 46-47 0.01941173243316996 1110 48-49 0.07384686680826036 1110 50-51 0.5615564982746832 1110 52-53 -0.48002780656796773 1110 54-55 -0.7719290919905077 1110 56-57 0.822998792302748 1110 58-59 -2.6791998736383675 1110 60-61 -0.22328216920498534 1110 62-63 0.35338670164875907 1110 64-65 0.5761734989570968 1110 66-67 0.390277098350694 1110 68-69 1.2972493975642045 1110 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-0.08070715524183925 2117 4 -2.339562418125812 2117 5 -4.570056975110667 2117 6 -2.744783882231342 2117 7 -3.4629399265230845 2117 8 -3.494137139479399 2117 9 -0.5576938847343555 2117 10-11 -0.9815988101332422 2117 12-13 -1.1346945329984095 2117 14-15 -4.30286790895434 2117 16-17 -3.3118605875669047 2117 18-19 -4.282046411855472 2117 20-21 -2.705142704516291 2117 22-23 -1.4941783940185438 2117 24-25 -2.569115721527993 2117 26-27 -2.0787379886685216 2117 28-29 -2.335728680605108 2117 30-31 -3.1271726002951965 2117 32-33 -1.106184343827124 2117 34-35 -3.033237371885903 2117 36-37 -3.833398907984531 2117 38-39 -2.690766964213992 2117 40-41 -2.6647654183242224 2117 42-43 -2.9525584171870634 2117 44-45 -1.9309454775277288 2117 46-47 -1.687731124709682 2117 48-49 -3.2142483712869776 2117 50-51 -2.6574911207729386 2117 52-53 -2.6824087589489167 2117 54-55 -3.621929091990509 2117 56-57 -3.504382160078208 2117 58-59 -2.348247492685985 2117 60-61 -3.1506631215859393 2117 62-63 -3.7978037745417126 2117 64-65 -2.814302691519096 2117 66-67 -3.9132943302207366 2117 68-69 -2.869417269102456 2117 70-71 -2.797100409490003 2117 72-73 -2.3690476562946507 2117 74-75 -1.5935858876665385 2117 76 -1.898301418424765 2118 1 -0.3263757765919806 2118 2 -1.1374770869745383 2118 3 0.8910877165530344 2118 4 0.4501811716177784 2118 5 -0.8264672315209225 2118 6 -1.8883736258210853 2118 7 -0.06293992652308589 2118 8 -7.432598677940938 2118 9 -6.098719525759996 2118 10-11 -2.2162141947486305 2118 12-13 -0.02828427658815258 2118 14-15 -4.20543201151844 2118 16-17 -1.7413477670540871 2118 18-19 -2.1576874374964987 2118 20-21 -2.515399114772702 2118 22-23 -1.601870701710851 2118 24-25 -1.7998849522972264 2118 26-27 -1.4607892707198005 2118 28-29 -1.1254722703486948 2118 30-31 -0.7374290105516081 2118 32-33 -1.698492036134816 2118 34-35 -1.3409296795782097 2118 36-37 -1.4705783951640186 2118 38-39 -1.5407669642139936 2118 40-41 -1.2378423414011444 2118 42-43 -2.7948661094947553 2118 44-45 -3.3809454775277317 2118 46-47 -3.350551637530195 2118 48-49 -3.786043243081849 2118 50-51 -2.403644966926784 2118 52-53 0.6060527795126163 2118 54-55 -0.6565444766058945 2118 56-57 1.0789511732551276 2118 58-59 -2.932862877301371 2118 60-61 -3.7750220959449123 2118 62-63 -1.6516499283878687 2118 64-65 -1.0181488453652463 2118 66-67 -4.201755868682277 2118 68-69 -0.9591608588460474 2118 70-71 -3.649664512054109 2118 72-73 -2.5998168870638807 2118 74-75 -3.0397397338203866 2118 76 -1.4700962902196402 2119 1 0.5646498644336582 2119 2 -1.1919642664617172 2119 3 -1.6057071552418378 2119 4 -4.181229084792477 2119 5 -1.2367236417773313 2119 6 -2.9781172155646765 2119 7 -5.687939926523086 2119 8 -1.9358038061460654 2119 9 -2.4576938847343577 2119 10-11 -6.935765476799908 2119 12-13 -4.930527866331737 2119 14-15 -4.165367908954337 2119 16-17 -4.58269392090024 2119 18-19 -7.694546411855473 2119 20-21 -5.896809371182961 2119 22-23 -4.273345060685209 2119 24-25 -5.173282388194657 2119 26-27 -4.095404655335187 2119 28-29 -4.619062013938439 2119 30-31 -5.743839266961864 2119 32-33 -3.8603510104937904 2119 34-35 -3.5124040385525674 2119 36-37 -5.004232241317865 2119 38-39 -5.978266964213994 2119 40-41 -5.377265418324221 2119 42-43 -6.623391750520398 2119 44-45 -4.360112144194396 2119 46-47 -5.233564458043016 2119 48-49 -4.580915037953641 2119 50-51 -4.640824454106273 2119 52-53 -4.744908758948917 2119 54-55 -6.501095758657172 2119 56-57 -6.650215493411537 2119 58-59 -5.319080826019324 2119 60-61 -3.550663121585938 2119 62-63 -4.518637107875051 2119 64-65 -5.051802691519093 2119 66-67 -4.496627663554069 2119 68-69 -6.536083935769124 2119 70-71 -4.317933742823339 2119 72-73 -5.5148809896279865 2119 74-75 -7.214419220999872 2119 76 -5.514968085091432 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 6.0 13 23.0 14 102.0 15 249.0 16 381.0 17 443.0 18 424.0 19 328.0 20 304.0 21 252.0 22 239.0 23 236.0 24 226.0 25 249.0 26 313.0 27 382.0 28 445.0 29 559.0 30 697.0 31 931.0 32 1280.0 33 1667.0 34 2542.0 35 4029.0 36 6654.0 37 5210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.219842969307635 21.991434689507493 11.381156316916488 34.407566024268384 2 16.477353400539542 18.990487008377112 39.539258838563114 24.992900752520235 3 16.502200766718726 27.537980974016758 28.425386908987647 27.534431350276872 4 12.444980832031804 18.15632542950447 36.71730796535567 32.681385773108055 5 11.944483884708221 34.534289365327275 34.81470964077808 18.70651710918643 6 24.953854891381514 39.34402953286952 17.81556155047565 17.88655402527332 7 24.893511287803495 30.356382223484314 23.95641062047423 20.793695868237965 8 21.088314638648303 34.21482322873776 21.961522078659662 22.735340053954282 9 22.60755359931847 16.477353400539542 22.61465284679824 38.30044015334374 10-11 22.987363339486013 25.461451086184866 28.026054238250747 23.525131336078374 12-13 22.353755501916797 23.313928723555303 26.824506602300158 27.50780917222774 14-15 19.916938804486726 25.6158597188698 24.675209427800652 29.791992048842825 16-17 21.595910833451654 27.445690756779783 25.985020587817694 24.973377821950873 18-19 20.470680107908564 27.25046145108618 26.28141417009797 25.997444270907284 20-21 20.245279000425956 27.527332102797104 26.12168110180321 26.105707794973732 22-23 21.606559704671305 26.650575039045858 26.85822802782905 24.884637228453784 24-25 21.368734914099104 28.100596336788303 26.1128070424535 24.417861706659096 26-27 19.4572625301718 28.602868095981826 27.49183586539827 24.448033508448106 28-29 21.23384921198353 27.55040465710635 28.25145534573335 22.96429078517677 30-31 21.068791708078944 27.720786596620755 27.319679114013915 23.890742581286382 32-33 19.368521936674714 28.76615078801647 27.27530881726537 24.590018458043446 34-35 20.791921056368025 28.384566235978987 27.573477211415593 23.2500354962374 36-37 20.980051114581855 27.781130200198778 27.316129490274033 23.922689194945335 38-39 20.80611955132756 27.933764021013772 28.061550475649582 23.198565952009087 40-41 21.06346727246912 27.316129490274033 28.020729802640922 23.59967343461593 42-43 20.783046997018317 27.514908419707513 27.80065313076814 23.901391452506036 44-45 20.158313218798806 27.656893369302853 27.71368734914099 24.471106062757347 46-47 20.9055090160443 27.33565242084339 27.729660655970466 24.029177907141843 48-49 20.076671872781485 27.797103507028254 28.453783898906714 23.672440721283543 50-51 20.589592503194662 26.895499077097828 27.94263808036348 24.57227033934403 52-53 20.373065455061763 26.950518245066025 28.105920772398125 24.570495527474087 54-55 20.133465852619622 27.605423825074542 27.931989209143833 24.329121113162007 56-57 20.52214965213687 27.13864830327985 27.972809882152493 24.366392162430785 58-59 20.243504188556013 27.046358086042883 27.97103507028255 24.739102655118558 60-61 20.064248189691895 26.87952577026835 27.955061763453077 25.10116427658668 62-63 19.27623171943774 27.07830469970183 28.517677126224623 25.12778645463581 64-65 19.96485872497515 28.088172653698706 27.7012636660514 24.245704955274743 66-67 20.18316058497799 27.88051966491552 27.388896776941646 24.547422973164846 68-69 19.19636518529036 27.55217946897629 27.55217946897629 25.699275876757067 70-71 20.273675990345026 27.113800937100667 27.644469686213263 24.968053386341047 72-73 19.7927019735908 27.193667471248045 27.47408774669885 25.5395428084623 74-75 19.854820389038764 27.87164560556581 26.899048700837714 25.374485304557716 76 20.133465852619622 26.4908419707511 27.17236972880875 26.203322447820533 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.5 15 2.5 16 2.5 17 3.0 18 2.0 19 3.5 20 8.5 21 11.0 22 11.0 23 18.0 24 25.5 25 26.0 26 50.0 27 91.5 28 146.5 29 184.0 30 219.0 31 279.5 32 412.5 33 520.0 34 577.5 35 727.5 36 872.0 37 924.0 38 1042.0 39 1312.0 40 1649.5 41 1829.5 42 1824.0 43 1868.5 44 2011.5 45 2062.0 46 2014.0 47 2029.5 48 1938.5 49 1706.0 50 1580.0 51 1467.0 52 1230.5 53 1049.0 54 991.0 55 903.0 56 692.0 57 508.5 58 448.0 59 366.5 60 270.5 61 211.5 62 167.0 63 139.0 64 100.0 65 75.5 66 53.5 67 45.0 68 43.5 69 42.5 70 32.5 71 22.0 72 21.5 73 15.5 74 9.0 75 8.0 76 8.5 77 6.5 78 5.0 79 6.0 80 6.0 81 3.5 82 1.0 83 1.0 84 0.5 85 1.0 86 2.0 87 2.0 88 1.0 89 0.5 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.539542808462303 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 28172.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.83075394008236 #Duplication Level Percentage of deduplicated Percentage of total 1 79.25326182249496 54.55061763453074 2 10.510030426486514 14.468266363765443 3 4.631014388118199 9.562686355246344 4 2.2433087514826466 6.1763453073974155 5 1.4078696302408333 4.845236404941076 6 0.7580836470527564 3.1307681385773103 7 0.4641328451343406 2.2362629561266507 8 0.22690939095456655 1.2494675564390176 9 0.216595327729359 1.3417577736759903 >10 0.288793770305812 2.4385915093000143 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.007099247479767145 0.0 21 0.0 0.0 0.0 0.007099247479767145 0.0 22 0.0 0.0 0.0 0.021297742439301433 0.0 23 0.0 0.0 0.0 0.021297742439301433 0.0 24 0.0 0.0 0.0 0.024847366179185006 0.0 25 0.0 0.0 0.0 0.024847366179185006 0.0 26 0.0 0.0 0.0 0.02839698991906858 0.0 27 0.0 0.0 0.0 0.03194661365895215 0.0 28 0.0 0.0 0.0 0.03549623739883572 0.0 29 0.0 0.0 0.0 0.039045861138719294 0.0 30 0.0 0.0 0.0 0.04259548487860287 0.0 31 0.0 0.0 0.0 0.04614510861848644 0.0 32 0.0 0.0 0.0 0.053244356098253585 0.0 33 0.0 0.0 0.0 0.06034360357802073 0.0 34 0.0 0.0 0.0 0.0638932273179043 0.0 35 0.0 0.0 0.0 0.07454209853755502 0.0 36 0.0 0.0 0.0 0.10648871219650717 0.0 37 0.0 0.0 0.0 0.1526338208149936 0.0 38 0.0 0.0 0.0 0.19522930569359648 0.0 39 0.0 0.0 0.0 0.2733210279710351 0.0 40 0.0 0.0 0.0 0.3833593639074258 0.0 41 0.0 0.0 0.0 0.5537413034218372 0.0 42 0.0 0.0 0.0 0.7738179752946188 0.0 43 0.0 0.0 0.0 0.9903450234275167 0.0 44 0.0 0.0 0.0 1.1926735766008802 0.0 45 0.0 0.0 0.0 1.4375976146528469 0.0 46 0.0 0.0 0.0 1.693170523924464 0.0 47 0.0 0.0 0.0 2.009087036774102 0.0 48 0.0 0.0 0.0 2.2788584410052533 0.0 49 0.0 0.0 0.0 2.5592787164560558 0.0 50 0.0 0.0 0.0 2.7687065171091865 0.0 51 0.0 0.0 0.0 3.0349282976004543 0.0 52 0.0 0.0 0.0 3.4715320176061337 0.0 53 0.0 0.0 0.0 3.8158455203748405 0.0 54 0.0 0.0 0.0 4.195655260542383 0.0 55 0.0 0.0 0.0 4.632258980548062 0.0 56 0.0 0.0 0.0 4.95882436461735 0.0 57 0.0 0.0 0.0 5.281840124946756 0.0 58 0.0 0.0 0.0 5.5551611529177904 0.0 59 0.0 0.0 0.0 5.878176913247196 0.0 60 0.0 0.0 0.0 6.22958966349567 0.0 61 0.0 0.0 0.0 6.502910691466704 0.0 62 0.0 0.0 0.0 6.953712906431918 0.0 63 0.0 0.0 0.0 7.3938662501774814 0.0 64 0.0 0.0 0.0 7.865966207581996 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTGAGA 15 0.0022028089 69.996445 50 TCTGGTG 15 0.0022028089 69.996445 47 GGTCTGG 15 0.0022028089 69.996445 45 GTCTGGT 15 0.0022028089 69.996445 46 GAATGGC 15 0.0022028089 69.996445 64 CTGGTGA 15 0.0022028089 69.996445 48 TGGGGCT 15 0.0022028089 69.996445 21 ACGGTTG 15 0.0022028089 69.996445 44 TAAGGTG 15 0.0022028089 69.996445 5 TATGGCT 15 0.0022028089 69.996445 4 CGCCTAG 25 2.351645E-4 55.997158 36 GGAGTTT 25 2.351645E-4 55.997158 59 TTGTCTG 20 0.0068835295 52.497334 26 GTTTGGG 20 0.0068835295 52.497334 18 GTCATTA 20 0.0068835295 52.497334 68 GGGTCGC 20 0.0068835295 52.497334 32 TGTCTGG 20 0.0068835295 52.497334 27 AGGGTAT 20 0.0068835295 52.497334 17 TAATGTC 20 0.0068835295 52.497334 64 ATAATTG 20 0.0068835295 52.497334 22 >>END_MODULE