FastQCFastQC Report
Wed 25 May 2016
SRR1780590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28172
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT1750.6211841544796252RNA PCR Primer, Index 47 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC720.2555729092716172No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC300.10648871219650717No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCAT150.002202365570.035
ACGTCTC200.006882148352.558
TAAAAGG200.006882148352.54
TACGTCT200.006882148352.557
TCTCGAG200.006882148352.561
GTCTCGA200.006882148352.560
CGAGTGA200.006882148352.564
TTCAGCC200.006882148352.544
TTTGTAG200.006882148352.535
CTCGAGT200.006882148352.562
CTGCTTG552.9204317E-431.81818451
TTTTTTC450.00423812831.11111324
AGGAGTA450.00423812831.11111321
CTTCTGC604.863305E-429.16666648
TTAGGGT500.007093374627.9999984
TCTGCTT657.767088E-426.92307750
CTTGAAA657.767088E-426.92307754
TCTTCTG657.767088E-426.92307747
TGCTTGA700.001197075925.052
GCTTGAA700.001197075925.053
Produced by FastQC (version 0.11.5)